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Estradiol-17β를 처리한 흰쥐 자궁에 있어서 Clomiphene이 세포내 수용체, DNA 및 cAMP 농도에 미치는 영향
김곤섭,고복현 동국대학교 경주대학 1989 論文集 Vol.8 No.-
These experiments were carried out to investigate the study on the interaction of estradiol receptor and cAMP level of clomiphene in immature rat uterus. The results obtained were summarized as follow : 1. The binding affinity of clomiphene to estradiol receptor in uterine cytosol was lower than that of estradiol-17β. clomiphene inhibited the replenishment of the receptor to cystol in uterus(P<0.01). 2. DNA level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01) 3. cAMP level in uterus was increased at 48 hours after estradiol-17β or clomiphene injection(P<0.01)
A Study on Ocular and Skin Irritation of a New Disinfectant, Clean Natural<SUP>®</SUP>
Suk Kim,Myung Sun Chun,Hee Sik Chung,Won Chul Jung,Dong Hyeok Kim,Jeong Ju Lim,Ho Yeong Shon,Gon Sup Kim,Hu-Jang Lee 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.1
This test was performed to evaluate the ocular and skin irritation of Clean Natural<SUP>®</SUP>", a new disinfectant of which main components are propolis and wood vinegar from Quercus mongolica. The results of eye irritation test were as followings; in the single application test, 0.1 ㎖ Clean Natural<SUP>®</SUP>" eyedrops and 0.9% saline as vehicle control were applied to one eye of rabbit, respectively. The reactions were examined for 7 days after treatment. Transient, Up to 2 days, eye irritation was observed in the rabbit eyes treated with the test solution. The results of skin irritation test were as following; there were no observed clinical signs, body weight changes and gross pathologic findings during experimental period. There were no observed erythema, eschar, and edema formation on intact and abraded skin. The primary irritation index (P.LL) of Clean Natural<SUP>®</SUP>" was 0 and was evaluated none irritating product. In the present study, 10% Clean Natural<SUP>®</SUP>" was evaluated as minimally irritating and none-irritating product for eye and skin, respectively.
Munki Kim, Chong-Sup Kim, Gon-Sup Kim, Chung-Kil Won 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.3
The morphology of the lingual papillae in a female Bengal tiger (Panthera tigris tigris) was examined by scanning electron microscopy (SEM). The tongue was 22.3 cm in length and 7.1 cm in width. Numerous filiform papillae were distributed over the entire dorsal surface of the tongue. SEM examination of the tongue revealed two types of mechanical papillae, i.e. filiform and conical papilla, and two types of gustatory papillae, i.e. fungiform and vallate papilla, on the dorsal surface of the tongue. Each filiform papilla consisted of one primary papilla and several secondary papillae. The filiform papillae on the anterior part of the tongue were divided into one primary and 6~14 secondary papillae. Unlike other mammalians, however, secondary papillae in the mid-part of the tongue showed pineal-like papillae. In the posterior part of the tongue, secondary papillae were rare or absent. Fungiform papillae were surrounded by filiform papillae and densely distributed on the lingual surface. There were two vallate papillae on the borderline between the lingual body and root of the tongue. A vallate papilla contained two secondary papillae inside the grooves. Conical papillae were located in the area of the vallate papillae and covered the posterior part of the tongue root. No foliate papillae were seen on both margins of the posterior part of the tongue. Our results indicate that the structure on the lingual papillae of the Bengal tiger is somewhat different from that of other mammals.
Young-Suk Kim,Boh-Hyun Kim,Gon-Sup Kim,Joung-Soon Jang,So-Young Kim,Byeong-Dae Choi,Jeong-Ok Kim,Yeong-Lae Ha 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.4
Glycoproteins isolated from fruit bodies and mycelial cultures of mushrooms exhibit anti-carcinogenic actions in human cancer cells and animal tumor cells by induction of apoptosis. Here, we report that isoflavone-conjugated glycoproteins (designate Gluvone), exhibit strong anti-carcinogenic effects on human breast cancer MCF-7 cells by induction of apoptosis. Gluvone with 9.4 kDa of molecular weight was isolated from submerged-liquid culture of Agaricus blazei mycelia (ABM) in soy flake-containing liquid medium. MCF-7 cells were incubated with various amounts of Gluvone (0~250 μM) for a period of 6 days. Gluvone exhibited anti-proliferative actions in a dose-dependent manner and 62% growth inhibition at 200 μM for 4 days relative to control. Hoechst 33258 staining analysis revealed that Gluvone induced formation of apoptotic bodies. Gluvone was associated with down-regulation of anti-apoptotic Bcl-2 protein expression as well as up-regulation of pro-apoptotic Bax protein expression. Gluvone treatment induced proteolytic activation of caspase-9 and caspase-3 through cytochrome c release from mitochondria to cytosol as well as concomitant degradation of poly (ADP-ribose) polymerase (PARP). In addition, Gluvone induced activation of caspase-8. Taken all together, these results indicate that the anti-proliferative effect of Gluvone is associated with induction of apoptotic cell death through the mitochondrial dysfunction pathway mediated by enhancement of Bax protein expression and suppression of Bcl-2 protein expression.
Gon-Sup Kim , Arulkumar Nagappan, Hyeon-Soo Park, Kwang-Il Park, Jin-A Kim, Gyeong-Eun Hong, Silvia Yumnam, Eun-Hee Kim, Won-Sup Lee, Wang-Jae Lee, Myung-Je Cho, Woo-Kon Lee, Chung-Kil Won 충북대학교 동물의학연구소 2013 Journal of Biomedical and Translational Research Vol.14 No.2
Vitamin C (ascorbic acid) is an essential nutrient of most living tissues. We established a strain of Gulo-/- mice with known deficiency, in which vitamin C intake can be controlled by diet, like humans, and investigated the differentially expressed proteins following treatments with Helicobacter pylori and diethylnitrosamine (DENA) in the liver of Gulo-/- mice using a proteomic approach. Expression of p53, 14-3-3ε and 14-3-3δ in Gulo-/- mice liver tissue was analyzed by immunohistochemistry. 2-DE maps constructed from Gulo-/- mice liver and differentially expressed proteins in liver tissue were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS). In Gulo-/- mice after H. Pylori infection, followed by treatment with DENA, no differences in p53, 14-3-3ε and 14-3-3δ were observed by immunohistochemistry. Proteome analyses using MALDI-TOF/MS resulted in successful identification of 12 proteins (nine proteins were up-regulated and three were down-regulated). Specifically, peroxiredoxin-6 and Alpha-1-antitrypsin 1-4 were up-regulated in liver after H. Pylori infection followed by treatment with DENA. These results indicated that oral supplementation with vitamin C led to rescue of Gulo-/- mice from vitamin deficiency, and protected the liver from H.pylori infection and/or DENA effect, and vitamin C also protected the liver against oxidative stress
Acute and chronic effects of repeated 1 Hz rTMS on the temporal cortex
Kim, Won Sup,Lee, Mina,Han, Joo Man,Kim, Song E.,Kim, Hee-Jin,Kim, Bom Sahn,Kim, Byung Gon,Lee, Hyang Woon Lippincott Williams Wilkins, Inc. 2012 NEUROREPORT - Vol.23 No.9
Electroencephalography amplitude, phase synchronization, and directionality of phase coupling within and between hemispheres were compared for different frequency components in 27 healthy individuals before and after 5 days of daily 1 Hz repetitive transcranial magnetic stimulation (rTMS), and at 2 weeks after the last session. Instantaneous amplitudes of α (8–13 Hz) and &bgr; (13–30 Hz) frequency components were increased after daily rTMS, the effects of which were declining over time, suggesting an adapting response with repeated rTMS sessions. The phase synchronization of electroencephalography increased significantly in the α frequency, especially the upper-α band (11–13 Hz), in both the frontal and the temporal areas, predominantly in the ipsilateral hemisphere. Asymmetric directional interactions of the upper-α band were stronger from the stimulated area to the contralateral hemisphere. No significant differences were found at 2 weeks after rTMS in any of these values. Focal 1 Hz rTMS induces an enhancement in the ipsilateral dominant corticocortical interaction drastically by interhemispheric asymmetric coupling from the stimulated cortical area with an adapting response with repeated sessions. This kind of method can be valuable for possible clinical applications in various neuropsychiatric conditions to study the therapeutic mechanisms of 1 Hz rTMS.
Gyu-Hyen Cho, Munki Kim, Chong-Sup Kim, Gon-Sup Kim, Chung-Kil Won 충북대학교 동물의학연구소 2014 Journal of Biomedical and Translational Research Vol.15 No.3
Cytokeratin (CK) comprises the intermediate filament cytoskeleton of epithelial cells. Patterns of CK expression can be regarded as a specific marker for epithelial differentiation status. The aim of this study was to identify CK expression on tongues of Korean native goats ranging from 60-day-old fetuses to newborns during prenatal development using immunohistochemistry. The tongues of fetuses were removed from 2- to 4-year-old female Korean native goats by caesarean section performed under general anesthesia. Immunohistochemistry was performed to assess CK expression patterns on developing goat tongues using serial paraffin-embedded sections. Light zones signifying CK immunoreactivity in dorsal lingual epithelia were weakly positive in 60-day-old fetuses. In 90-day-old fetuses, deep areas in dorsal lingual epithelia were strongly positive for CK expression and superficial areas were moderately positive. In 120-day-old fetuses, light zones of lingual epithelia in the vallate papilla were strongly positive for CK expression, whereas ducts of von Ebner’s glands were moderately positive. In neonates, taste buds were positive for CK expression, whereas non-taste epithelial cells and von Ebner’s glands were negative. These findings indicate that goat tongues have different patterns of CK expression during development and provide a morphological basis for studies on the biological mechanism of epithelial differentiation.
Chungkil Won, Gyuhyen Cho, Munki Kim, Gon-Sup Kim, Chongsup Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.4
The aim of this study was to investigate the morphological development pattern of conical papilla (CP) during prenatal and postnatal periods in native Korean goats by scanning electron microscopy. Tongues were removed from fetuses on days 90, 120, and neonates and from juveniles on days 30, 50, 60, 75, 90, 120, 150, and 180 after birth. The lengths of CP were 194~240 μm in neonates, 335~485 μm in the weaning period of 60-day-old goats, and 526~662 μm in the maturing period of 180-day-old goats. The primordia of CP in 90-day-old fetuses, shaped like mountain berry, were sprouted. The CP of 120-day-old fetuses was a cylinder shape with an apex whose center was slightly concave and the margin was sticking up like a low fence. The CP of neonates was empty inside and the secondary papillae were irregularly arranged fence-like structures on it. In 60-day-old goats, CP was an obliquely sectioned cylinder shape compacted in inner surface; the shapes of CP varied in 120-day-old goats, and had a smooth surface. A low elevation of CP was observed in 150- and 180-day-old goats. The microridges, microplicae, and micropits were well developed on the epithelial surface of lingual papillae in 60- to 120-day-old goats. These findings indicate that CP of goats has a variety of sizes and shapes during development.
한방사료첨가제를 이용한 육계의 Salmonella 방제효과 - 항균성 및 장관정착 억제 -
강호조,김용환,이후장,김종수,김종섭,김도경,김은희,박미림,김곤섭,Kang, Ho-Jo,Kim, Young-Hwan,Lee, Hu-Jang,Kim, Jong-Su,Kim, Chong-Sup,Kim, Toh-Gyong,Kim, Eun-Hee,Park, Mi-Rim,Kim, Gon-Sup 대한수의학회 2003 大韓獸醫學會誌 Vol.43 No.1
In this study, antibacterial activity on the chicken feed supplemented with different concentrations of oriental herbal medicine feed additives (OHMFA) extract was tested for some organisms and their preventive effects on the colonization of Salmonella enteritidis (SE) in broiler chickens were examined. The growth of Bacillus cereus, listeria monocytogenes and Staphylococcus aureus was inhibited on the feed of 0.25% OHMFA and Salmonella spp. and Escherichia coli O157:H7 were inhibited on the feed containing 2.0% level. The broiler chickens fed a forage additived 1.0% level of OHMFA after inoculation of a $10^7CFU/ml$ of SE were increased in weight than the chicken fed forage without OHMFA. The incidence of SE in liver, spleen and cecum of the chickens fed forage with OHMFA was decreased on the 7th days of postinfection and SE was not isolated from the organs of the chickens after the 14th days of postinfection. Serum antibody titers of the chickens were lowered than the control group.