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Park, Byong-Gon,Chun, Joo-Mi,Lee, Chang-Jin,Chun, Gie-Taek,Kim, Ik-Hwan,Jeong, Yeon-Ho The Korean Society for Biotechnology and Bioengine 2000 Biotechnology and Bioprocess Engineering Vol.5 No.2
A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis menium ion. The semi-fed-batch operation was employer to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.
김지운 ( Ji Un Kim ),이용승 ( Yong Seung Lee ),문성오 ( Seong O Moon ),배덕수 ( Duk Soo Bae ),이제호 ( Je Ho Lee ),김병기 ( Byong Gie Kim ),박지영 ( Ji Young Park ),박창수 ( Chang Soo Park ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.4
Primary squamous cell carcinoma of the endometrium is very rare. In 1928 Flumann suggested three criteria for diagnosis: (1) no coexisting endometrial adenocarcinoma, (2) no connection between the endometrial squamous cell carcinoma and squamous epitheliu