PPARγ 배위자에 의해 유도된 종양성장 억제와 비장세포의세포 살해능 증가

장규윤,유기훈,이학용,김경열,최하나,차은정,박호성,문우성,강명재,이동근 대한병리학회 2007 Journal of Pathology and Translational Medicine Vol.41 No.1

Background : Recent studies have proposed the use of peroxisome proliferator activated receptor-γ (PPARγ) ligands as new chemotherapeutic agents for human malignant tumors. However the in vivo mechanism of PPARγ ligands on cellular toxicity is not clear. Therefore we examined the anti-tumor effects of the PPARγ ligand, rosiglitazone (ROS), in animal models. Methods : To evaluate the effect of RSO on splenocytes, an in vitro and in vivo study was performed. Cytolytic activity was measured by use of a 51Cr release assay. The splenic natural killer (NK) cell population and effector-target conjugation were measured by flow cytometric analysis. Results : In 9L glioma bearing rats, 30 mg/kg/d of ROS treatment induced a significant decrease of subcutaneous tumor growth accompanied by an increased cytolytic activity of splenocytes and of the splenic NKR-P1bright/CD3- NK cell population. In normal rats, systemic administration of ROS also increased the cytolytic activity of splenocytes, the splenic NK cell population, and effector-target conjugation. Moreover, we found that a concentration of 20 μM ROS caused an increase in the cytolytic activity of splenocytes, and a concentration of 50 μM ROS increased effector-target conjugation in vitro. Conclusion : These results suggest that increased splenic cytolytic activity and NK cell population may contribute to the anti-tumor effects of PPARγ ligands in vivo. However, the roles of NK cells in the PPARγ ligand- induced anti-tumor activity should be further investigated.

직장의 선암과 동반된 항문주위 파제트 병 : 1예 보고 A case report

장규윤,김경선,윤한상 의과학연구소 1999 全北醫大論文集 Vol.23 No.1

Extramammary Paget's disease most commonly occurs on the female external genitalia and rarely occurs in the perianal region. Recently we experienced a case of perianal Paget's disease associated with rectal adenocarcinoma. The patient was 59-year-old man. Barium enema and abdominal CT scan showed an ulcerative lesion in the rectum. Lower anterior resection was performed. Histologically, the tumor was composed of an invasive, moderately differentiated adenocarinoma extending through the muscularis propria. There were prominent pagetoid spread of signet ring tumor cells within the squamous epithelium of the anal canal and perianal skin, in direct continuity with the adjacent rectal tumor. Intraepithelial gastrointes-tinal-type gland was also recognized. In both the rectum and the skin specimen, the neoplastic cells were positive for PAS, mucicarmine, alcian blue, cytokeratin, and carcinoembryonic antigen. The presence of intraepithelial glands with staining characteristics suggestive of gastrointestinal adenocarcinoma probably represents pagetoid spread of underlying rectal adenocarcinoma.

흰쥐 초산 유도 십이지장 궤양 치유과정에서 TGF-beta 및 그 수용체의 발현

장규윤,윤한상,유기훈,정명자,이동근,강명재 대한병리학회 2003 Journal of Pathology and Translational Medicine Vol.37 No.2

Background : Many works have been written about the transforming growth factor-beta 1 (TGF-β1) which is closely associated with fibrosis in the inflammatory conditions of different organs. TGF-β1 exerts its biological effects by interacting with specific cell surface receptors, namely, transforming growth factor-beta receptor type I and II (TGF βRI and TGF βRII). Methods : To investigate the temporal expressions and localizations of TGF-β1, TGF βRI, and TGF βRII in acetic acid-induced duodenal ulcerated tissues, we performed in situ hybridization and immunohistochemical techniques. Results : Under in situ hybridization, TGF-β1, TGF βRI, and TGF βRII mRNA signals increased in the experimental groups (1, 3, 7, and 14 day groups) compared to those of the control group. The signals on day 14 decreased slightly compared to those of days 1, 3, and 7, but they were higher than those of the control group. Under immunohistochemical study, TGF-β1, TGF βRI, and TGF βRII were localized in the mucosal epithelial cells and in the macrophages, vascular endothelial cells, and fibroblasts of the lamina propria and granulation tissue. As in the case of the in situ hybridization, it revealed that the expression of three proteins increased in the experimental groups compared to that of the control group. The expression on day 14 decreased compared to those of days 1, 3, and 7, but it was more intense than that of the control group. Conclusions : This study suggests that TGF-β1, TGF βRI, and TGF βRII contribute to the early stage healing of duodenal ulcer.

Intraosseous Neurilemmoma of the Mandible - A Case Report -

장규윤,문우성,박호성 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.1

Neurilemmoma (Schwannoma) is a benign nerve sheath tumor that’s composed entirely of welldifferentiated Schwann cells. Intraosseous neurilemmomas are rare and they represent less than 1% of all benign primary bone tumors. We report here on an additional case of intraosseous neurilemmoma that was located in the mandible of a 77-year-old woman. CT revealed an expansile, well-defined lesion on the right side of the mandibular body with thinning of the cortex. The lesion was surgically removed and it was found to be a 2×1.7 ㎝-sized, bright yellowish, hard mass with hemorrhage and cyst formation. Histologically, the mass was a moderately cellular neoplasm and it showed distinct nuclear palisading, numerous Verocay bodies and tumor cells that were positively immunohistostained for S-100 protein. Two months after the operation, the patient has remained in a good condition with no signs or symptoms of tumor recurrence. Neurilemmoma (Schwannoma) is a benign nerve sheath tumor that’s composed entirely of welldifferentiated Schwann cells. Intraosseous neurilemmomas are rare and they represent less than 1% of all benign primary bone tumors. We report here on an additional case of intraosseous neurilemmoma that was located in the mandible of a 77-year-old woman. CT revealed an expansile, well-defined lesion on the right side of the mandibular body with thinning of the cortex. The lesion was surgically removed and it was found to be a 2×1.7 ㎝-sized, bright yellowish, hard mass with hemorrhage and cyst formation. Histologically, the mass was a moderately cellular neoplasm and it showed distinct nuclear palisading, numerous Verocay bodies and tumor cells that were positively immunohistostained for S-100 protein. Two months after the operation, the patient has remained in a good condition with no signs or symptoms of tumor recurrence.

복막에 발생한 거대 고립성 섬유성 종양 : 1예 보고

장규윤,이미숙,김상호 의과학연구소 1998 全北醫大論文集 Vol.22 No.1

Solitary fibrous tumor (SFT) is a rare spindle cell neoplasm of adults that usually arises in the pleura recently reported in other locations. We report a giant SFT of the peritoneum in a 27-year-old woman. The tumor measured as 50×40×40cm in size and 24kg in weight. Histologically, the tumor demonstrated the calssic features of a benign SFT. The patient is under regular follow-ups till now(a period of 15 months after the operation) without any evidence of recurrence, or metastasis. To our knowledge, this lesion is the largest SFT that has been reported. Despite the large size, this lesion was easily resected without any complication and did not show any malignant feature.

자궁 경부 상피내 종양과 침윤성 암증에서 인체 유두종바이러스 감염과 p 53 단백 발현의 상관성에 관한 연구

장규윤,박승창,이호,이동근,정명자,최호열 의과학연구소 1998 全北醫大論文集 Vol.22 No.1

In order to identify the correlation between the incidence of HPVs infection and the expression of mutant gene of p53 protein in carcinogenesis of uterine cervical carcinomas, immunohistochemistry for mutant gene of p53 protein and in situ hybridization for HPV 16/18 along with each histologic subtype of uterine cervical carcinomas were performed on 51 cervical carcinomas : including 40 cases of squamous cell carcinomas, 8 cases of adenocarcinomas, 2 cases of small cell carcinomas, and 1 case of adenosquamous carcinoma. The results are summarized as follows : 1. The detection rate of HPV 16/18 by in situ hybridization was 19 of 51 cases(37.3%). 2. In respect to relationship between HPV 16/18 gemone and the histologic subypes, the positive rates of in situ hybridization for HPV16/18 were 18 of 40 squamous cell carcinomas(45.0%) and 1 of 8 adenocarcinomas(12.5%), while all the other subtypes of cervical cancer were negative. 3. The positive rates of immunohistochemistry for p53 protein were only 2.0%(1 of 51 cases). The positive case was the invasive squamous cell carcinoma, while all adenocarcinomas were negative 4. In aspect to the relationship between HPVs infection and the expression of mutant gene of p53 protein, the one case of positive for p53 protein was the invasive squamous cell carcinoma with positive for HPV 16/18. All negative samples for HPV 16/18 showed the negative for p53 protein. These results revealed that mutation of p53 gene and HPV 16/18 is associated with the carcinogenesis of uterine cervical carcinoma. However, determination of underlying basis of tumor development in large number of HPV negative cervical cancer requires further works for other carcinogenic mechanism.

단방낭성 과립막세포종 -1예 보고-

장규윤,정명자,문우성,강명재,이동근 대한병리학회 2004 Journal of Pathology and Translational Medicine Vol.38 No.4

Unilocular cystic granulosa cell tumors (UCG) are extremely rare. Due to the relatively small mass of the tumor available for histologic examination, diagnosis of UCG is not easy. Here we present a case of UCG in a 54-year old female. A 12×10 cm unilocular cystic mass was identified in the right ovary. The tumor was thin-walled and consisted of a single large cavity with a smooth internal surface. In most areas there were no cells lining the cyst, however, in focal areas the unilocular cyst was lined by one or more layers of uniform granulosa cells, forming Call-Exner bodies. A diagnosis of UCG was made after multiple sections were examined. A careful histologic examination is required for the correct diagnosis of UCG.

症例(증례) : 난소의 성숙 낭기형종에서 발생한 편평상피세포암 1예 보고

장규윤 ( Kyu Yun Jang ),정명자 ( Myoung Ja Chung ),문우성 ( Woo Sung Moon ),강명재 ( Myoung Jae Kang ),이동근 ( Dong Geun Lee ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.1

성숙 낭기형종은 대부분 양성종양이지만 드물게 구성조직 중 하나가 악성종양으로 전환하며 외배엽에서 기원한 편평상피세포암이 가장 흔하다. 양성 난소 기형종의 악성 변화는 주로 60세 이후 폐경여성에서 발생하며 예후는 매우 나쁜 것으로 알려져 있다. 그러나 수술 이외의 특별히 정립된 치료법은 없는 상태이다. 최근 저자들은 87세 여성의 좌측 난소에서 발생한 성숙 낭기형종에서 기원한 편평상피세포암 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Mature cystic teratoma (MCT) is a common ovarian tumor. But malignant transformation of an ovarian MCT is rare, and it is reported to be less than 2%. A variety of malignant tumors have been reported, including adenocarcinomas, malignant melanomas, sarcomas, thyroid papillary carcinomas, and carcinoid tumor. The most common cancer is a squamous cell carcinoma(SCC), but it is rare. We experienced a case of squamous cell carcinoma arising in mature cystic teratoma of the left ovary in a 87 year old woman. A total hysterectomy and bilateral adnexectomy with omentectomy were performed. Grossly, left ovarian tumor was cystic tumor containing solid lesion. Histologically, the cyst wall lined by SCC in situ. Areas of invasion were noted. In solid area, the tumor was predominantly composed of keratinized malignant squamous cells. We reported this case with brief review of literatures.

증례(症例) : 자궁의 지방평활근종 1예 보고

장규윤 ( Kyu Yun Jang ),최근혜 ( Geun Hae Choi ),양선호 ( Sun Ho Yang ) 전북대학교 의과학연구소 2004 全北醫大論文集 Vol.28 No.2

자궁의 지방평활근종은 평활근과 함께 성숙한 지방세포가 섞여있는 매우 드문 중 간엽 종양으로 대부분 50-70 세의 폐경 후여성에서 발생하는 것으로 알려져 있다. 지방평활근종은 자궁 평활근종의 변형으로 알려져 있으며 치료에 있어서는 평활근종과 다르지 않다. 최근 저자들은 자궁에서 발생한 지방평활근종 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. 환자는54세 여자로 전자궁 적출술을 시행하였다. 육안소견상 자궁 체부의 근층에서 6.2×5cm 크기의 주변조직과 분명한 경계를 이루며 회백색 부위와 지방성의 황색 부위가 불규칙하게 섞여 있는 종괴가 관찰되었다. 조직학적 소견 상 종괴는 방추상의 평활근 세포와 지방 조직이 불규칙하게 섞여 있었다. 면역조직화학염색 소견상 방추상의 평 활근 세포는 smooth muscle actin에 양성이었고 지방 조직은 S-100 단백과 smooth muscle actin에는 양성이었다. Uterine lipoleiomyoma is a rare mesenchymal neoplasm consists of mixed smooth muscle and adipose tissue and tend to occur in middle-aged or elderly women. Lipoleiomyma mostly described as a variant of leiomyoma. We report a case of uterine lipoleiomyoma in a 54-year-old woman with a brief review of the literatures. The tumor measured 6.2×5 cm and was located in the corpus uteri, Grossly, the tumor was well circumscribed and showed white to bright yellow color and lobated with nodular whirling architecture. Histologically, mature adipose tissue intermingled with fibrous and smooth muscle components in an irregularly reticular pattern. Immunohistochemically, spindle shaped smooth muscle cells showed immunoreactivity for smooth muscle actin and adipose tissue showed immunoreactivity for S-100 protein and smooth muscle actin.

증예(症例) : 난소의 세르톨리 세포 종양 -1예 보고-

장규윤 ( Kyu Yun Jang ),정재신 ( Jae Sin Jeong ),최호열 ( Ho Yeul Choi ) 전북대학교 의과학연구소 2003 全北醫大論文集 Vol.27 No.2

Sertoli cell tumors of the ovary are rare. Here we presented a such case in 46-years old female. In left ovary. 7x6 cm sized solid tumor was resected. The tumor was yellow and solid. Histologically, the tumor showed tubular formation of columnar and cuboidal cells. The lumen were empty. Tumor cells had moderate to abundant cytoplasm and oval nuclei without nuclear atypia. A few mitotic figures were present. No Leydig cells or other types of neoplastic cells were seen in this tumor. The tumor cells were immunoreactive for cytokeratins.