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이예람 ( Ye Ram Lee ),손용준 ( Young Jun Son ),박수연 ( Soo Yun Park ),장은영 ( Eun Young Jang ),유지연 ( Ji Yeon Yoo ),손홍주 ( Hong Joo Son ) 한국환경과학회 2016 한국환경과학회지 Vol.25 No.6
Lactic acid bacteria (LAB) are industrially important microorganisms for probiotics. The recent widespread application of LAB for preparation of functional food is attributable to the accumulating scientific evidence showing their beneficial effects on human health. In this study, we isolated and characterized plant-derived LAB that show angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities. The selected strain K2 was isolated from Kimchi, and identified as Lactobacillus plantarum by 16S rRNA gene analysis. The strain grew under static and shaking culture systems. They were also able to grow in different culture conditions like 25℃∼37℃ temperature, 4∼10 pH range and ∼6% NaCl concentration. L. plantarum K2 was highly resistant to acid stress; survival rate of the strain at pH 2.5 and 3 were 80% and 91.6%, respectively. The strain K2 also showed high bile resistance to 0.3% bile bovine and 0.3% bile extract with more than 74% of survival rate. The cell grown on MRS agar plate containing bile extract formed opaque precipitate zones around the colonies, indicating they have bile salt hydrolase activity. The strain showed an inhibitory activity against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; antibacterial activity was probably due to the lactic acid. The K2 strain showed relatively higher autoaggregation values, antihypertensive and antioxidant activities. These results suggest that L. plantarum K2 could be not only applied as a pharmabiotic for human health but also is also starter culture applicable to fermentative products.
환경친화적 미생물농약으로서의 잠재성을 가진 세균의 분리 및 특성
이예람(Ye-Ram Lee),이상미(Sang-Mee Lee),장은영(Eun-Young Jang),홍창오(Chang-Oh Hong),김근기(Keun-Ki Kim),박현철(Hyean-Cheal Park),이상몽(Sang-Mong Lee),김용균(Young-Gyun Kim),손홍주(Hong-Joo Son) 한국생명과학회 2015 생명과학회지 Vol.25 No.12
화학농약의 오남용은 환경오염을 유발하고, 사람의 건강에 악영향을 미치므로 그 대안으로 미생물을 이용한 생물학적 방제에 대한 관심이 높아지고 있다. 본 연구에서는 환경에서 안정성을 유지할 수 있는 생물방제균을 확보하기 위하여 잿빛곰팡이병균인 Botrytis cinerea의 생육을 억제할 수 있는 Bacillus 속 균주를 탐색한 후, 선정된 균주의 항진균 물질 생산을 위한 조건, 항진균 기작 및 물리화학적 안정성에 대하여 조사하였다. 가장 높은 항진균 활성을 가진 균주는 고추의 근권 토양에서 분리된 AF4 균주였으며, 표현형적 특성 및 16S rRNA 유전자 염기서열에 근거하여 동정한 결과, Bacillus subtilis로 확인되었다. AF4 균주는 최적 탄소원으로 glycerol을, 최적 질소원으로 casein을 요구하였으며, 25-40℃ 및 pH 7-10에서 항진균 활성을 나타내었다. 최적조건에서 항진균 활성은 140±3 AU/ml로, 기본배지보다 활성이 증가했음을 알 수 있었다. AF4 균주는 B. cinereas 균사를 형태적으로 변화시킴으로써 항진균 효과를 나타내는 것으로 추정되었으며, 다양한 식물병원성 곰팡이의 생육을 억제할 수 있었다. 또한 AF4 균주가 생성하는 항진균 물질은 열, pH, 유기용매 및 단백질 분해효소 처리에도 항진균 활성을 유지하였으므로 대단히 안정한 물질인 것으로 판단되었다. 따라서 Bacillus subtilis AF4는 다양한 곰팡이유래 식물질병방제에 적용가능성이 높은 균주임을 알 수 있었다. In this study, to retain a stable bacterial inoculant, Bacillus strains showing antifungal activity were screened. The improved production, antifungal mechanism, and stability of the antifungal metabolite by a selected strain, AF4, a potent antagonist against phytopathogenic Botrytis cinerea, were also investigated. The AF4 strain was isolated from rhizospheric soil of hot pepper and identified as Bacillus subtilis by phenotypic characters and 16S rRNA gene analysis. Strain AF4 did not produce antifungal activity in the absence of a nitrogen source and produced antifungal activity at a broad range of temperatures (25-40°C) and pH (7-10). Optimal carbon and nitrogen sources for the production of antifungal activity were glycerol and casein, respectively. Under improved conditions, the maximum-antifungal activity was 140±3 AU/ml, which was higher than in the basal medium. Photomicrographs of strain AF4-treated B. cinerea showed morphological abnormalities of fungal mycelia, demonstrating the role of the antifungal metabolite. The B. subtilis AF4 culture exhibited broad antifungal activity against several phytopathogenic fungi. The antifungal activity was heat-, pH-, solvent-, and protease- stable, indicating its nonproteinous nature. These results suggest that B. subtilis AF4 is a potential candidate for the control of phytopathogenic fungi-derived plant diseases.
황병선,김정호,이예람,경찬욱,선준호,선영규,김진영,Hwang, Byungsun,Kim, Jeongho,Lee, Ye-Ram,Kyeong, Chanuk,Seon, Joonho,Sun, Young-Ghyu,Kim, Jin-Young 한국인터넷방송통신학회 2022 한국인터넷방송통신학회 논문지 Vol.22 No.5
Recently, interesting of home training is getting bigger due to COVID-19. Accordingly, research on applying HAR(human activity recognition) technology to home training has been conducted. However, existing paper of HAR proposed static activity instead of dynamic activity. In this paper, the deep learning model where dynamic exercise posture can be analyzed and the accuracy of the user's exercise posture can be shown is proposed. Fitness images of AI-hub are analyzed by blaze pose. The experiment is compared with three types of deep learning model: RNN(recurrent neural network), LSTM(long short-term memory), CNN(convolution neural network). In simulation results, it was shown that the f1-score of RNN, LSTM and CNN is 0.49, 0.87 and 0.98, respectively. It was confirmed that CNN is more suitable for human activity recognition than other models from simulation results. More exercise postures can be analyzed using a variety learning data.
연구논문 : 수산폐기물인 미더덕껍질 용매 추출물의 항산화 및 항고혈압 활성
이상미 ( Sang Mee Lee ),이예람 ( Ye Ram Lee ),조광식 ( Kwang Sik Cho ),박수연 ( Soo Yun Park ),장은영 ( Eun Young Jang ),황대연 ( Dae Youn Hwang ),정영진 ( Young Jin Jung ),손홍주 ( Hong Joo Son ) 한국환경과학회 2015 한국환경과학회지 Vol.24 No.7
Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and leads to environmental problems. It destroys the beach scenery and causes a bad smell and pollution by trashing on the seashore. Therefore, purpose of this study was to investigate antioxidant and antihypertensive activities of different solvent extracts from S. clava tunic for recycling of fishery waste. Antioxidant and antihypertensive activities of all extracts were concentration-dependent. Of extracts, hot water extract showed the highest DPPH radical scavenging activity with the lowest effective concentration (EC50) value (0.733 mg/ml). Chloroform extract exhibited the highest metal chelation activity with the lowest EC50 value (2.696 mg/ml). Autoclaved water extract showed the highest NO radical scavenging activity with the lowest EC50 value (0.491 mg/ml) and n-hexane extract showed the highest reducing power (A700=1.897 at 100 mg/ml). And n-butanol extract showed the highest SOD-like activity with the lowest EC50 value (19.116 mg/ml) and ACE inhibition activity with the lowest inhibitory concentration(IC50) value (0.149 mg/ml). These results indicate that extracts obtained from S. clava tunic may potential candidate to reduce diseases caused by various oxidative stresses and hypertension.
가금폐기물 처리를 위한 세균유래 케라틴 분해효소의 특성
고태훈 ( Tae Hun Go ),이상미 ( Sang Mee Lee ),조광식 ( Kwang Sik Cho ),이예람 ( Ye Ram Lee ),박수연 ( Soo Yun Park ),장은영 ( Eun Young Jang ),정성윤 ( Seong Yun Jeong ),손홍주 ( Hong Joo Son ) 한국환경과학회 2014 한국환경과학회지 Vol.23 No.6
Keratin wastes are generated in excess of million tons per year worldwide and biodegradation of keratin by microorganisms possessing keratinase activity can be used as an alternative tool to prevent environmental pollution. For practical use of keratinase, its physicochemical properties should be investigated in detail. In this study, we investigated characteristics of keratinase produced by Xanthomonas sp. P5 which is isolated from rhizospheric soil of soybean. The level of keratinase produced by the strain P5 increased with time and reached its maximum (10.6 U/ml) at 3 days. The production of soluble protein had the same tendency as the production of keratinase. Optimal temperature and pH of keratinase were 40℃-45℃ and pH 9, respectively. The enzyme showed broad temperature and pH stabilities. Thermostability profile showed that the enzyme retained 94.6%-100% of the original activity after 1 h treatment at 10℃-40℃. After treatment for 1 h at pH 6-10, 89.2%-100% of the activity was remained. At pH 11, 71.6% of the original activity was retained after 1 h treatment. Although the strain P5 did not degrade human hair, it degraded duck feather and chicken feather. These results indicate that keratinase from Xanthomonas sp. P5 could be not only used to upgrade the nutritional value of feather hydrolysate but also useful in situ biodegradation of feather.
페놀분해세균 Rhodococcus pyridinovorans P21의 분리 및 페놀분해 특성
조광식(Kwang-Sik Cho),이상미(Sang-Mee Lee),신명재(Myung-Jae Shin),박수연(Soo-Yun Park),이예람(Ye-Ram Lee),장은영(Eun-Young Jang),손홍주(Hong-Joo Son) 한국생명과학회 2014 생명과학회지 Vol.24 No.9
페놀과 각종 난분해성 화합물이 함유된 폐수를 미생물학적으로 처리하기 위하여 폭넓은 연구가 진행되고 있으나 이들 균주들은 200 ppm 이상의 고농도 페놀이 존재할 경우, 기질저해 현상에 따른 생육이 일어나지 않는 단점이 있다. 본 연구에서는 유류로 오염된 토양에서 고농도 페놀을 분해할 수 있는 P21 균주를 분리하였으며, 표현형 및 계통분류에 근거하여 동정한 결과, Rhodococcus pyridinovorans로 동정되었다. 본 균주에 의한 페놀분해 최적조건은 0.09% KNO₃, 0.1% K₂HPO₄, 0.3% NaH₂PO₄, 0.015% MgSO₄?7H₂O, 0.001% FeSO₄?7H₂O, 초기 pH 9 및 20-30℃이었으며, 이 조건에서 1000 ppm의 페놀을 2일 만에 완전히 분해하였다. 1,500 ppm의 페놀은 3일 만에 완전히 분해할 수 있었으나 그 이상의 페놀은 분해할 수 없었다. 또한 본 균주는 toluene, xylene 및 hexane과 같은 독성화합물을 이용하여 생육할 수 있었으며, chloroform에서는 생육할 수 없었다. The effluents of chemical and petroleum industries often contain non-biodegradable aromatic compounds, with phenol being one of the major organic pollutants present among a wide variety of highly toxic organic chemicals. Phenol is toxic upon ingestion, contact, or inhalation, and it is lethal to fish even at concentrations as low as 0.005 ppm. Phenol biodegradation has been studied in detail using bacterial strains. However, these microorganisms suffer from substrate inhibition at high concentrations of phenol, whereby growth is inhibited. A phenol-degrading bacterium, P21, was isolated from oil-contaminated soil. The phenotypic characteristics and a phylogenetic analysis indicated the close relationship of strain P21 to Rhodococcus pyridinovorans. Phenol biodegradation by strain P21 was studied under shaking condition. The optimal conditions for phenol biodegradation by strain P21 were 0.09% KNO₃, 0.1% K₂HPO₄, 0.3% NaH₂PO₄, 0.015% MgSO₄?7H₂O, 0.001% FeSO₄?7H₂O, initial pH 9, and 20-30℃, respectively. When 1,000 ppm of phenol was added to the optimal medium, the strain P21 completely degraded it within two days. Rhodococcus pyridinovorans P21 could grow in up to 1,500 ppm of phenol as the sole carbon source in a batch culture, but it could not grow in a medium containing above 2,000 ppm. Moreover, strain P21 could utilize toxic compounds, such as toluene, xylene, and hexane, as a sole carbon source. However, no growth was detected on chloroform.