결정성 Dihydroquerceting 의 새로운 분취방법 (分取方法)

송홍근 한국목재공학회 1991 목재공학 Vol.19 No.3

The new preparative separation technique of dihydroquercetin (2, 3-trans-dihdroxy-3, 3', 4', 5, 7-pentahydroxydihydroflavonol) was investigated by liquid chromatography. Also some typical coniferous wood bark were examined for the sources of dihydroquercetin. The good sources of dihydroquercetin were Douglas-fir 〔Pseudotsuga menziesii〕 bark and pitch pine 〔Pinus rigida〕 bark. There is no dependance the produced place and species dihyroquercetin which is separated by silica-gel column chromatography was taken with white needle-like crystals. This crystals were very stable in the humidity and on the light. It also can stored very long without derivatives.

한국산 참나무류에 함유된 cis-, trans- MO-lactone의 성분분석 연구

송홍근 건국대학교 생명과학연구원 1995 생명과학지 Vol.2 No.-

The MO-lactone(3-methyl-4-hydroxy octanoic acid γ-lactones) which is decisive compounds in flavor improvement during aging of whisky and/or brandy were extracted from 6 species of Quercus in Korea. These compounds were identified by GC and GC/MS and compared with these from the heartwood of mizunara(Q. mongohra Turez var. grosseserrata Rehd. et. Wils) in Japan. The amount of MO-lactones in 6 oak species were determined comparatively. The highest amounts of MO-lactone were showed in extract from Q. acutissima and others are in order of Q. serrata, Q. alliena, Q. dentata, Q. variabilis and Q. mongolica. Therefore these 6 oak species in Korea can be used possibly as raw materials of liquor aging cask. Q. acutissima was best possible species among them because it contained relatively hightest value of trans-MO-lactones.

옻칠의 정제기술에 관한 연구 (1) : 생산지 , 생산시기에 따른 생칠과 옻칠의 특성 및 도막 특성 Properties of Raw Lacquer , Refined Lacquer and Film according as Their Collecting Places and Seasons

송홍근,한창훈 한국목재공학회 2001 목재공학 Vol.29 No.1

In this study, we obtained fundamental data about Korean raw lacquer' s physical and chemical properties to produce high quality lacquer. The tested raw lacquers were obtained from Won-ju in Korea, Shanxishang, Guizhoushang, Anhuishang in China. The drying time of refining lacquers, tensile strengths of dried films and uniformity of films are measured. The refined lacquers were prepared by experimentally scaled refining equipment. Films of lacquer were applied on glasses by film applicator. This films were tested by universal strength test machine. The films were pictured by scanning electron microscopy and confocal microscopy to define the uniformity of them. The refining method were not different among three different kind of raw lacquers which were different their collecting time and places. But the viscosity of them were quite different. When black refined lacquer is made with iron powder, the adding time of iron powder is critical to control the viscosity of it. The refining times, viscosity and tensile strength of refined lacquers were not depended the method of refining condition but the place of collecting of raw lacquer.

한국산 참나무류에 함유된 cis-, trans- MO-lactone의 성분분석 연구

송홍근 건국대학교 생명과학연구원 1995 생명과학지 Vol.2 No.1995

잣나무 수피의 Stilbene glycosides 의 분리 및 동정

송홍근 한국목재공학회 2001 목재공학 Vol.29 No.4

EtOAc extract from the bark of Pinus koraiensis Sieb. et Zucc was isolated by column chromatography which was packed with Sephadex LH-20 or TSK-gel HW-40F. Several stilbene glycosides were identified by ¹H·(13)^C-NMR, HMQC, HMBC and FAB^+ MS. Three stilbene glycosides, Z-pinostilbenoside, E-desoxyrhaponticin, and E-resveratroloside, were identified.

소나무와 잣나무잎 정유성분의 생체학적 차이

송홍근 한국목재공학회 1995 목재공학 Vol.23 No.1

The terpenoids are one of the secondary metabolities in Pines species and functioned the protection them from insects or/and fungus etc. Since Pinus koraiensis has not any damage by Pine needle gall midge but Pinus densiflora has great injury by them in South Korea. It may great helf to find these reasons to analize their monoterpenenoids and to compare the seasonal variation of their amounts. Terpenoids were analized by GC, GC/MS. Some biogenetic analysis of terpenoids also was elucidated. The seasonal variation of terpenoid was distinguished at March and August and/or September. Most of major component of monoterpenes were increased or decreased in these periods in Pinus koraiensis. Especially 3-carene was showed significant variation between the period of physiological activity and the period of domain. The effect of infection by Pine needle gall midge was significant in Pinus densiflora for amount of monoterpene during the period of infection. The components of p-menthane series between Pinus densiflora and Pinus koraiensis was recogniz ed a significant difference during the experiment. Biogenetic cooccurrance of pmenthane series also was recognized between 3-careen and terpinolene in Pinus koraiensis.

소나무속 수목의 부위별 추출물의 항균활성

김종진,송홍근,한창훈 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4

This study was carried out to investigate the antifungal activities of the extracts from various parts of three Pious species, P. densiflora, P. rigida and P. koraiensis to pathogenic fungus Colletotrichum gloeosporioides. The EtOAc fraction from the bark of P. koraiensis stem and root showed 98.8 and 100% of activity, respectively to the fungus. Median effective doses (ED_(50)) of above two fractions were 469 and 588 ㎍/㎖, respectively in the bioassay with the fungus. ED_(50) of the EtOAc fraction from the bark of P. koraiensis stem against Alternaria brassicicola and Fusarium oxysporum was 533 and 2,277 ㎍/㎖, respectively. This means that the fraction was more sensitive to the C. gloeosporioides and A. brassicicola than the fungus F. oxysporum. The EtOAc fraction from the leaves of P. densiflora showed 39.6% of activity to C. gloeosporioides, but all the fractions from the leaves of two species showed no activity. The active compounds in the bark of P. koraiensis stem and root are being identified.

소나무와 잣나무의 잎과 수지에 함유된 정유 성분

김재광,송홍근 한국목재공학회 1994 목재공학 Vol.22 No.3

The essential oils of leaves and resins from P. densiflora and P. koraiensis were analyzed to identify their components. After each retention times of 45 known terpenoids were dertermined with a fixed analytical condition by GC the essential oil compounds of leaves and resins were identified by comparing their retention times with the retention times of known standards. To confirm these results the essential oil components of leaves from P. koraiensis were analized by 2 different GC/MS. According to these results, 36 terpenoids in essential oils of leaves from P. densiflora and P. koraiensis were identified and 25 terpenoids and 22 terpenoids were identified from P. koraiensis resin and P. densiflora resin, respectively. The major components which are more than 2% of total amaunt of volatile components were as followes: 1. The major terpenoids of leaves from red pine. α-pinene, camphene, β-pinene, D-limonene, β-phellandrene, myrcene, terpinolene, α-terpineol. 2. The major terpenoids of leaves from korean pine. α-pinene, camphene, myrcene, D-limonene, 3-carene, terpinolene, bornyl acetate, β-caryophyllene, α-terpineol, borneol, δ-cardinene. 3. The major terpenoids of resin from red pine. α-pinene, β-pinene, myrcene, β-phellandrene, linalool, linalyl acetate. 4. The major terpenoids of resin from korean pine. α-pinene, β-pinene, D-limonene, β-caryophyllene, phytol.

소나무수피 프로안토시아니딘 ((Proanthocyanidin) 의 분리 및 구조분석

오성진,송홍근 한국목재공학회 1996 목재공학 Vol.24 No.2

To elucidate the structure of procyanidin in Korean led pine(Pinus densiflora S. et Z.), bark, the extractives were extracted with acetone-water mixture(7 : 3. v/v) from inner bark of Korean red pine. The extracts separated three fractions which were extracted by liquid-liquid extraction. The extracting solvents were chloroform and ethyl acetate and water. The part of ethylacetate soluble was chromatographed by liquid chromatography. The ethylacetate soluble portion yielded four natural procyanidin dimers, two known epicatechin-(4β→6)-catechin, catechin-(4α→8)-catechin and two unknown catechin-(4β→6)-catechin and conformational isomer of epicatechin-(4α→6)-catechin. The additional catechins was also isolated. The structures of these procyanidins were elucidated by their ^(13)C-NMR spectra.

Assessment of Antioxidative Capacity in Relation to Seed Traits of Rice Varieties

정일민,송홍근,김광호,Joung-kuk Ahn,Sun-Joo Lee,Jin-Yeong Back 한국작물학회 2006 Korean journal of crop science Vol.51 No.7

In order to assess antioxidant capacity in relation to seed traits of rice (Oryza sativa L.), ninety-six varieties were examined for antioxidative activity of brown rice grain using superoxide dismutase (SOD), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and thiobarbituric acid (TBA) assays. Overall, average total activities measured by the three methods were of very wide range between 64% and 13%. Significant differences were noted depending on the variety and evaluation method. Rice varieties with foreign origin, middle maturity, colored hulls, and colorless awn exhibited statistically significant higher total activity. As for the measurements, total activity was significantly correlated with SOD (r = 0.29***), DPPH (r = 0.82***) and TBA (r = 0.76***). Between the three activities, SOD was not positively correlated with DPPH (r = -0.15*), while TBA was significantly correlated with DPPH value (r = 0.51***). DPPH (55.20%) and TBA (50.36%) were significantly higher in foreign rice, while SOD activity (44.29%) was significantly higher in domestic rice. However, an average total activity was significantly higher in foreign rice (47.31%) than in domestic rice (35.92%). SOD, DPPH and TBA activities of middle maturity in maturity time were the highest total activity (44.96%) and significantly differed from the other two groups. Total activity was significantly higher in rice with a colorless awn (42.18%) than with a colored awn (35.87%).