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Cyclosporine A 투여시 사람 치은섬유모세포에서 IL-6 발현 증가를 유도하는 신호전달경로에 관한 연구
성진효,김동기,하미숙 朝鮮大學校 口腔生物學硏究所 2002 口腔生物學硏究 Vol.26 No.1
Cyclosporine A(CsA) is a widely used immunosuppressant, with clinical applications ranging from organ transplants to chronic inflammatory diseases. One of the side effects associated with CsA treatment is the development of gingival overgrowth. Alghough CsA has been shown to stimulate IL-6 production in human gingiva fibroblast cells in culture, the signaling mechanism that regulate IL-6 production is not fully understood. The aim of this study was to examine the role of the p38 and extracelluar signal-regulated kinase(ERK) mitogen-activated protein kinase (MAPK) pathway in regulating CsA mediated IL-6 production from human gingiva fibroblast.Human gigiva fibroblast cultures were established from systemically healthy gingival tissue donors. Cells were treated for various time with CsA in the presence and absence of the specific p38 and ERK1,2 MAPK inhibitors SB203580 and PD98059. respectively, either untreated or treated. IL-6 levels in the cell culture media were measured b enzyme-linked immnosorbent assays. MAPK activation was demonstrated by immunoblot for the active kinase (tyrosing/threonine phosphorylated) in whole cell extracts using phospho-specific antibodies. p38 MAPK activity in human gingival fibroblast cells was measured using an in vitro immunokinase assay using ATP as production in human gingival fibroblast. The activation of the p38 and the ERK1.2MAPKs occured following CsA stimulation. Pretreatment with various inhibitors had effect on CsA stimulated p38 MAPK activity. PD98059 decreased CsA stimulated phosphorylation of ERK1.2. Additionally, Wortmannin and LY290042, a potent inhibitor of the PI3K-kinase were able to inhibit IL-6 activation, but they had no effect on IL-6 activation when stimulated by CsA. This study provides evidence that both the p38 and ERK MAPK pathways are important for the regulation of the production of IL-6 from the human gingival fibroblast in response to CsA.
성진효,서은주,정학균 朝鮮大學校 口腔生物學硏究所 2001 口腔生物學硏究 Vol.25 No.2
To investigate the periodontal status over 15-year-old residents, this study was conducted with a total of 1,510 subjects living in Gwangju and Chunnam Province using the community periodontal index of treatment need(CPITN) necessary for the supply of treatment for the whole citizens of a community or a certain human groups. CPINTN based upon the clinical assessment of 10 index teeth with a specially designed periodontal probe. The results were as follows; In urban area, the mean number of sextants with the healthy periodontal condition was 2.0 as highest score, while in rural area the mean number of sextants with caculus was 1.93 as highest score. 2. In urban area, the mean number of sextants with the healthy periodontal condition was highest score in subjects aged less than 29 had the mean number of sextants with calculus was highest score in subjects aged over 30, while in rural area, the subjects aged less than 19 had healthy periodontal condition as highest score, the subjects whose aged from 20 to 29 had the bleeding periodontal tissues as highest score and the subjects aged over 30 had calculus forming periodonal tissue as highest score. 3. The percentage of subjects with calculus was 47.24% in urban and 43.28% as highest score, respectively. 4. In the subjects aged less than 15, the percentage of subjects with the bleeding was 38.03% in urban area and the percentage of subjects with healthy periodontal condition was 48.6% in rural area as highest score. While in the subjects form 15 to 64, the percentage of subjects with calculus was highest score and the subjects the subjects aged more than 65 proved to be the highest in terms of shallow periodontal pockets.
Actinobacillus actinomycetemcomitans의 Lipopolysaccharide에 대한 단클론항체의 제작 및 특성
성진효,이영종,김동기,장기완 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.4
Actinobacillus actinomycetemcomitans(A.actinomyceremcomitans), a nonmotile, gram negative facultative anaerobic coccobacillus, is supposed to be an important etiological agent in localized juvenile periodontitis and associated with adult type periodontitis. Lipopolysaccharide(LPS) is one of the various virulent factors of A. actinomycetemcomitans and can directly or indirectly cause destruction of periodontal tissue. The purpose of this study was to produce and characterize the monoclonal antibody to LPS of A.actinomycetemcomitans. The results were as follows; 1. The LPS yield of A. actinomycetemcomitans was 0.7% based on dry cell weight. 2. The antibody titers of immuninzed serum with 5.2 × 10^(8) bacteria were 1:64,000 to whole cells and 1:8,000 to LPS. 3. From three times of cloning the antibody producing hybridomas using limitation dilution method, ten monoclones producing monoclonal antibody to LPS of A.actinomycetemcomitans were obtained. 4. Ten monoclonal antibodies to LPS of A. actinomycetemcomitans were not found to cross-react with LPS of Escherichia coli(E. coli). These results indicate that the monoclonal antibody to LPS of A. actinomycetemcomitans was specific for A. actinomycetemcomitans.
성진효,박선화,김동기 대한구강보건학회 2001 大韓口腔保健學會誌 Vol.25 No.4
The purposes of this study were to obtain basic data for dental caries experience and to utilize these data for motivating oral health promotion and activating oral health care programme in preschool children of metropolitan. The data of dental caries experience of pre-school children aged from 3 to 5 years in Gwangju city were obtained from the chart for dental health assessment surveyed from 1993 to 1996. The subjects were 2,787 children in 1993, 2,625 in 1994, 3,523 in 1995 and 1,633 in 1996, respectively. The items of survey were inputted and analysed and then, calculated df, dt, ft, and dft rates and dft index in deciduous dentition, and DF, DT, Fr, MT, and DMFT rates and DMFT index in permanant dentition. The results were as follows; 1. Average percentages were 83.4% in df rates, 51.1% in dt rates, 49.0% in ft rates and 29.0% in dft rates. 2. An average score of dft index was 5.60. 3. Average percentages were 10.5% in DF rates, 62.8% in DT rates, 37.3% in FT rates, 0.0% in MT rates and 11.8% in DMFT rates. 4. An average score of DMFT index was 0.18.
성진효,손현아,윤은경 朝鮮大學校 口腔生物學硏究所 2001 Oral Biology Research (Oral Biol Res) Vol.25 No.2
In order to provide a guide for developing appropriate countermeasure against illegal dental treatment, a study was conducted to collect dat about oral health status of people with dental care by those who has no licence of dentist in Gwangju & Chunnam province. Among 942 subjects surveyed in Gwangju & Chunnam province, an 264 experienced people with dental care by illegal dentist were selected by questionnaire and dental surveyed. The results were as follows: 1. The percentage of people treated by illegal dentist in Gwangju & Chunnam province was higher than that of the whole korea. 2. The satisfaction of the prosthese by illegal dentist was uncomfortable approximately a half(50.3%). 3. The status of prostheses made by illegal dentist was mostly bad(77.7%). 4. Dental caries prevalence was higher in groups treated by illegal dentist, expecially, missing tooth caused by dental caries(p<0.05). 5. The percentage of people having prothesis was some higher in groups treated by illegal dentist, especially rural area(p<0.05). 6. The percentage of people needing prothesis was lower in groups treated by illegal dentist(p<0.05).
치학전문교육이 광주보건대학 보건계열 여학생의 구강건강과 구강환경관리에 미치는 영향에 대한 연구
성진효,김동섭,송요환,장기완 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.2
This study was designed to evaluate the effect of professional education in dentistry on the oral health and oral hygiene performance. The subjects were 320 female students who were collected from three health departments in Gwangju Health College. Oral Examination was conducted by one dentist during two months from April to May in 2000. The items of oral examination were dental caries prevalence, periodontal status measured by CPI (community periodontal index) and plaque control record by O' Leary. The obtained results were as follows ; 1. The mean number of permanent teeth with active dental caries was 0.9, the lowest in 3rd grade of department of dental hygiene(p<0.01). 2. The total score of CPI in 3rd grade of department of dental hygiene was lower twice than other groups(p<0.001). 3. The plaque control records in 3rd grade of department of dental hygiene was 54%, lower from 13 to 20% than other groups(p<0.05). 4. The plaque control records in proximal surface and buccal and lingual surface of molar teeth were over 60% and 50%, respectively in 3rd grade of department of dental hygiene. We concluded that oral health and oral hygiene performance was improved by the professional education in dentistry.