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Chlorella Protein의 營養評價에 關한 硏究〔Ⅱ〕 : 人工消化率과의 比較 Comparative of Artificial Digestion Ratio
朴忠雄,黃鎬觀 全北大學校 1974 論文集 Vol.16 No.-
The excellency of nutritional value in Chlorella Protein had already been proved, in the previous report of mine, by animal metabolic test. This time, by artificial digestive test, the following results have been obtained : 1) The artificial digestive ratio is increased by 70%, when 1(one) mg of Pepsin is acted on 1(one) gram of sample. 2) The digestive ratio is increased by 10% when the sample is homogenized. From this, we could see that the digestive ratio for Chlorella Protein is dependent upon the previous processes. 3) The artificial digestive test after the process of homogenation has shown us that Chlorella, when cultured by the synchronous culture method, is good in its quality, though its harvest per unit area is small in quantity.
박충웅 한국미생물학회 1990 微生物과 産業 Vol.16 No.1
이글은 전북대학교 유전공학연구소의 연구소설립 취지및 목표, 기구및 임원, 연구시설, 연구소 활동상황, 연구소 운영방향및 중장기 발전계획에 대해 소개하는 글이다.
朴忠雄 全北大學校 文理科大學 1977 論文集 Vol.5 No.-
This experiment was discussed in term of varing the absorption of Atomic Absorption Spectrophotometry due to the analysis of initial sample, the effect of pH, and interference of other elements. It was reported that the absorbance of potassium was increased and the absorbance of calcium, magnesium and zinc was decreased because of interference of Cu^++ and Fe^++, that the absorbance of potassium, calcium, magnesium and zinc was varied with effect of pH. The results obtained in this experiment are summarized as follows : 1. Optimal condition of pH in paddy soils was neutral state. 2. The absorbance of potassium was increased and the absorbance of calcium, magnesium and zinc was decreased by adding of Fe^++ and Cu^++ in the initial sample solution.
Saccharomyces sp. 균주들을 이용한 감즙으로부터 알콜 생성에 대한 연구
朴忠雄,李康民,金景淑 全北大學校 1993 論文集 Vol.36 No.-
By using the Korea persimmon juice as raw material, persimmon wine fermentation was carried out with 5 strain of Saccharomyces (S. cerevisiae, S. formosensis, S. sake, S. calsberg, S.ellipsoideus). S.cerevisiae and S. formosensis are very suitable for conversion of persimmon juice to persimmon alcohol but not S. sake, S. calsberg, S. ellipsoideus. In the process of fermentation, the compositions of amino acids were changed. Aromatic amino acids such as phenylalanine and tyrosine were decreased but arginine and methionine were increased. Amounts of sugars(glucose, fructose) and Saccharomyces affect on persimmon alcohol concentration produced. Persimmon alcohol concentration was double with adding of 10% glucose in S. formosensis fermentation. In S. cerevisiae fermentation, glucose could not augment the alcohol concentration. Finally we got 11.5% persimmon alcohol by S. formosensis. We designed a alcohol bioreactor by immobilization of S. formosensis on porous supporter and packing it in column.
박충웅,김종면,조인호 한국수산학회 1983 한국수산과학회지 Vol.16 No.1
The concentrations of dissolved vitamin B_12,thiamine and biotin in the water of Gyokpo coast, were determined by microbiological assay methods. Also the relations between the distribution of B group vitamin and other environmental factors were studied. Vitamin B_12 was assayed with Euglena gracilis strain Z, thiamine with Cryptococcus albidus and biotin with Achromobacter sp. yH-51. It was found that the concentration of B group vitamin in the water of Gyokpo coast were normal level : vitaminB_12; 1.36-3.95 ng/ℓ, thiamine ; u-0.4 ng/ℓ and biotin ; 1.40-14.60 ng/ℓ. The concentration of B group vitamin was high in summer than in winter. In the water of Gyokpo coast during summer, B group vitamin occurred slightly lower level than normal, the concentration suficiently neccessary for phytoplankton development. The concentration of biotin was positively correlated with abundance of phytoplankton. but no aerobic heterotrophic bacteria. It was suggested that the concentration of biotin in water might be much more influenced with the growth of phytoplankton and any environmental factors than bacteria and the other vitamin, especially.
Vitreoscilla Hemoglobin 유전자가 재조합 대장균의 성장조건에 미치는 영향
박충웅,박기인 한국유전학회 1994 Genes & Genomics Vol.16 No.4
In this rearch plasmid pMK79 was engineered by pMK57 which contain Bacillus stearothermophilus α-amylase gene into pUC8 and pUC8: 15 which has Vitreoscilla hemoglobin gene (VtHb). Both pMK57 and pMK79 were transformed into Escherichia coli. The presence of Vitreoscilla hemoglobin expressed VtHb improves the growth of MK79 (pMK79 transformant) in an oxygen limited environment compared to strain MK57 (pMK57 trasformant) as using efficiently oxygen. Expression of the amylase gene in both systems was also measured. The result showed that amylase production in MK79 was also significantly higher in mid log and late log phases than in MK57. It was also found that α-amylase gene transcription can occur from the B. stearothermophilus promoter as well, at induced levels, from the lac promoter in pUC8.
Chlorella Protein의 營養價에 관한 硏究 (Ⅱ)
박충웅(Chung-Ung Park),황호관(Ho-Kwan Hwang) 한국식품영양과학회 1974 한국식품영양과학회지 Vol.3 No.1
It was reported that the digestion ratio of chlorella was low because it had a low metabolic rate in body.<br/> Generally, the thickness of a cell membrane of it is 200-250 Å, the weight of it is approximatly 13% of the total weight of a dry cell. And it is composed of protein, lipid, hemicellulose and ash etc.<br/> So, in order to elevate the digestion ratio of chlorella in body, we experimented the crude treatment methods of chlorella.<br/> The results obtained in this experiment are summarized as follows :<br/> 1. The digestion ratios calculated from ordinary N- balance method were 83.05% for 10% chl. (b) plus diastase group ; 81.25% for 10% chl. (b) plus amylase group, and 79.23% for 10% chl. (b), 58.55% for 10% chl. (a).<br/> 2. Biological values from this method were 80.25% for 10% chl. (b) plus diastase group, and 60% for 10% for chlorella(a).
뱀 筋肉 Adenosine Triphosphate - Creatine Phosphotransferase에 關한 硏究
박충웅(Chung-ung Park) 한국식품영양과학회 1980 한국식품영양과학회지 Vol.9 No.1
A detailed procedure was described for the isolation of cratine kinase (ATP-Creatine phosphotransferase, E. C. 2. 7. 3. 2.) from the muscle of the snake Bungarus fasciatus. The original isolation procedure of Kuby et al. for the rabbit muscle enzyme has been modified and extended to include a chromatographic step.<br/> The properties of the enzyme have been investigated and kinetic constants for the reverse reactions determined as the followings:<br/> 1) A molecular weight of the enzyme was determined by gel filteration on Sephadex G-100 and by electrophoresis on SDS-polyacrylamide was 86,000.<br/> 2) Two reactive sulphydryl groups were detected with dithiobis nitrobenzoic acid (DTNB).<br/> 3) The nucleotide substrate specificity in the reverse reaction was determined as ADP*2'-dADP>GDP>XDP>UDP with magnesium as the activating metal ion.<br/> 4) The order of the metal specificity in the reverse reaction Mg>Mn>Ca~Co was determined with ADP as substrate.<br/> 5) A detailed kinetic analysis was carried out in the reverse direction with MgADP ̄ as the nucleotide substrate. Initial velocity and product inhibition studies(MgATP^(2-) competitive with respect to MgADP- and noncompetitive with respect to N-phosphorycreatine^(2-) ; Creatine competitive with respect to N-phosphorylcreatine^(2-) and noncompetitive with respect to Mg ADP ̄) indicated that the reaction obeyed a sequential mechanism of the rapid equilibrium random type.