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Aspergillus nidulans FGSC 159의 carboxymethylcellulases의 분리 순화 및 그 성질에 관한 연구
맹필재,홍순우,하영칠 한국미생물학회 1980 미생물학회지 Vol.18 No.3
Washed mycelia of Aspergillus nidulans FGSC159 were incubated in CMC minimal liquid medium and the culture filtrate which contained induced extracellular cellulase was fractionated by a three-step procedure including chromatography on Bio-Gel P-150, chromatography on DEAE-Sephadex A-50 and chromatography on Sephadex G-100. Three CMCase components ; F-I-Ia, F-I-Ib and F-II-Ia were prepared. No enzyme activity toward avicel could be detected in these components. Similarly, there was no ${\beta}-glucosidase$ activity. pH-optima of the three components were all 5.0 in acetate buffer. Temperature-optima for the activities of F-I-Ia, F-Ib and F-II-Ia were $45^{\circ}C,\;40^{\circ}C\;and\;50^{\circ}C$, respectively. F-II-Ia was shown to be more thermostable than the other two components. F-II-Ia was proved to have quite a different substrate specificity and action property and action property from those of F-I-Ia and F-I-Ib by product analysis on liquid chromatography.
맹필재,권태익 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.1
Two bacterial strains, BTF1 and BTF2, and one yeast strain, BTF3, were isolated from the starter of fermented black tea customarily called "Black tea fungus". The morphological, physiological and biochemical characteristics of the strains were analyzed. Consequently, the bacterial strains, BTF1 and BTF2, were identified as Acetobacter pasteurianus subsp. xylinum and Acetobacter pasteurianus, respectively, and the yeast strain, BTF3, were as Schizosaccharomyces pombe.
맹필재(Pil-Jae Maeng),장재현(Jae-Hyeon Jang),김근율(Kun-Yul Kim),유영문(Young-Moon Yu),김종수(Jong-Su Kim),김종태(Jong-Tae Kim) 한국조명·전기설비학회 2014 조명·전기설비학회논문지 Vol.28 No.8
This paper dealt with the LED optical illumination system design for the Masthead navigation light to replace halogen lamps. We made Fresnel lens satisfy luminous intensity distribution of “Convention on the International Regulation for Preventing Collisions at Sea(COLREG)”. The optical system is designed by classifying three parts: light source, lens, and cut off plate. The source of light has been made to have the uniform horizontal and vertical light distribution by placing 6 LEDs at intervals of 54°, and as the cylindrical Fresnel lens, the lens has been designed to achieve the uniform horizontal and vertical light distribution in the range of plain light. Finally, the cover has been designed to block the light from the outside of plain light and ultimately met the standards for light distribution of navigation lights. In addition, the validity of design has been verified with manufacturing a trial product.
Kang, Myoung Kyu,Maeng, Pil Jae,Rhee, Young Ha 충남대학교 생물공학연구소 1997 생물공학연구지 Vol.5 No.-
The weight average molecular weight of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesized by Alcaligenes sp. SH-69 was altered between 3.2 × 10 exp (5) and 1.1 × 10 exp (6) depending upon various culture conditions. It appeared that culture conditions favorable for the efficient production of copolyesters promoted the formation of higher molecular weight copolyesters. Polydispersity indices of isolated copolyesters were in the range of 1.5 to 2.5.
이정헌,맹필재 충남대학교 생물공학연구소 2003 생물공학연구지 Vol.9 No.1
On exposure to the harmful environmental stress, all living cells display a rapid molecular response which is commonly designated stress response. In this report, we are describe the mechanism of osmostress response mainly in fungi, including accumulation of compatible solutes, such as glycerol and trehalose, and the enzymes involved in the biosynthetic process of the osmolytes, such as glycerol-P dehydrogenase, dihydroxyacetone kinase, trehalose-6-P synthase, and trehalose-6-P phosphatase. We also describe the genes for the enzymes for osmolyte synthesis and transport, such as GPD1, GPD2, PTS1, PTS2, GUT1, HAL1, ENAI, ENA2; and the mechanism of the regulation of their expression including the signal transduction pathway, in which the Ras/cAMP/PKA pathway, Msn2/4p, and STRE are included.
김리라,맹필재 충남대학교 생물공학연구소 2007 생물공학연구지 Vol.13 No.-
Cell growth is an important phenomenon for self-renewal, organogenesis, and tumor expansion. This cell growth is tightly regulated on cell cycle by very complex and huge amount factors. Cell cycle is consisted by G (Gap) phase, M (mitosis) phase, and S (Synthesis), which contain specific regulation factors, cyclin and cyclin-dependent kinase (cdk). In other mechanism to regulate cell cycle, cell cycle-checkpoints indicate steps to regulate cell cycle on DNA damage checkpoint and spindle checkpoint. Fission yeast has been used as good model system for investigation of eukaryotic cell cycle. We have known the complete fission yeast genomic sequence and haven plentiful biochemical and proteomic information. On the basis of the information, we can make several mutants to analysis the roles of cell cycle regulatory factors and identify new factors. Here, we introduce how to use yeast model to discover the relation between mutants and cell cycle phenotypes and briefly explain the results from our functional study.
Purification and Characterization of Nonmitochondrial Citrate Synthase from Saccharomyces cerevisiae
Cho, Nam Seok,Kim, Kwang Soo,Maeng, Pil Jae 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Saccharomyces cerevisiae 세포로부터 citrate synthase 1 (mitochondrial) 및 citrate synthase 2 (nonmitochondrial)를 각각 분리·정제하고 citrate synthase 2의 물리학적 및 효소학적 특징을 citrate synthase 1에 대하여 비교·분석하였다. 정제된 두 동위효소는 모두 동일한 하부단위체로 구성된 dimer 단백질임이 확인되었으며, 각 하부단위체의 분자량은 citrate synthase 1의 경우 48.3 kDa, citrate synthase 2의 경우 47.0 kDa으로 각각 나타났다. 최적 반응 pH는 7.5로서 서로 유사하였으나, 최적 반응 온도는 citrate synthase 2의 경우 40℃로서 citrate synthase 1의 경우보다 25℃나 낮게 나타났다. 또한, citrate synthase 2는 열 및 pH에 대하여 citrate synthase 1보다 크게 떨어지는 안전성을 보였으며, Zn^(2+), Mn^(2+), Co^(2+) 등의 금속 이온에 의한 활성의 저해도 citrate synthase 1의 경우보다 더 크게 받는 것으로 밝혀졌다. 두 효소의 활성을 조절할 것으로 기대되는 몇몇 대사산물들의 영향을 조사한 결과, 그들 중 ATP는 두 효소 모두에 대하여 가장 강력한 저해효과를 보였으며, ADP와 NADH는 citrate synthase 1보다 citrate synthase 2에 대하여 더 강한 저해효과를 나타내었다. 두 동위 효소의 kinetics를 조사한 결과, citrate synthase 2의 acetyl CoA 및 oxaloacetate에 대한 친화력은 각각 citrate synthase 1의 1/7 및 1/3.5 정도에 불과한 것으로 확인되었다. Citrate synthase 1 (mitochondrial) and citrate synthase 2 (nonmitochondrial) were purified from Saccharomyces cerevisiae. The physical and enzymatic characteristics of citrate synthase 2 were analyzed in comparison with citrate synthase 1. Both isoenzymes were shown to be dimeric proteins of identical subunits, and the molecular weights of the subunits were estimated to be 48.3 kDa for citrate synthase 1 and 47.0 kDa for citrate synthase 2, respectively. The optimal pH value for enzyme activity was pH 7.5 for both isoenzymes. However, the optimal temperature for the activity was strikingly different; while the activity of citrate synthase 1 reached its peak at 65℃, that of citrate synthase 2 was maximal at 40℃. Citrate synthase 2 showed much lower thermal and pH stability than citrate synthase 1. In addition, citrate synthase 2 was affected much more by the metal ions such as Zn^(2+), Mn^(2+) and Co^(2+) than citrate synthase 1. Among the several possible regulatory metabolites tested, ATP showed the strongest inhibitory effect on both enzymes. ADP and NADH were found to have greater effect on citrate synthase 2 than on citrate synthase 1. Kinetic analysis revealed that citrate synthase 2 has approximately 7- and 3.5-fold lower affinity to acetyl CoA and to oxaloacetate, respectively, than citrate synthase 1.
Triton X-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향
박희문,민경렴,맹필재,하영칠 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-
Triton Ⅹ-100이 Trichoderma koningii의 성장 및 형태에 미치는 영향을 조사하기 위하여, Triton Ⅹ-100 첨가 여부에 따른 각 종 성장계수의 변화를 조사하여 보았다. 액체 배양시 Triton Ⅹ-100의 첨가에도 불구하고, specific growth rate과 doubling time은 변화가 없었으나, Triton Ⅹ-100을 첨가한 경우, 군체의 방사상 성장률이 감소하였다. 이러한 현상은 Triton Ⅹ-100에 의하여 균사의 분지 형성이 촉진되고, 격막형성이 촉진되어 격막 간의 간격이 짧아져 나타나는 것으로 확인되었다. We investigated the effect of Triton Ⅹ-100 on the growth and morphology of Trichoderma koningii by comparing various parameters representing the growth of mold in the presence or absence of Triton Ⅹ-100. The specific growth rate and doubling time of T. koningii were not affected by the addition of 0.05% Triton Ⅹ-100 into solid medium also resulted in decrease in the colony radial growth rate and this response was correlated with the formation of mycelia which showed increase in branching and septation in the presence of Triton Ⅹ-100.