유아에서 발생한 재발성 외음부 콘딜로마 1례의 치료

홍정희,문성택,김윤숙,전섭,최승도,선우재근,배동한 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

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針葉樹 幼苗의 모잘록병 發生에 미치는 環境要因의 影響

韓茂錫,尹貞求 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.2

The pathogens causing damping-off of coniferous seedlings were isolated from diseased seedlings of the pine, larch and soil of seedlings bed. After isolating and identifying the causal fungi, ecological factors such as temperature affecting mycelial growth, pathogenicity and conidia germination were investigated. In addition, outbreak of damping-off by different growing stages were also investigated. The results obtained were as follows. 1. The causal pathogens identified were Fusarium moniliforme, F. acuminayum, F. graminearum, the unidentified Fusarium spp. and confirmed their pathogenicity on the seedlings of Pinus densiflora and Pinnus thunbergii. 2. Fusarium moniliforme showed the highest frequency among pathogens isolated from diseased seedings, whereas in the soil, F. acuminatum and F. sp. (8706) showed higher frequency than the others. 3. The optimum temperature for mycelial growth the Fusaria was 25℃~30℃, mycelial growth of every species was discontinued at 5℃, the optimum PH for mycelial growth was at 5.5 to 7.5. 4. Optimum temperature for the conidial germination was at 25℃. At 100% of relative humidity the conidial germination was above 94%, but decreasing trend was shown at less than 90%. 5. The percentage of the diseased seedlings in Pinus densiflora and Larx kaempferi by the fertilizer application were not remarkably different, but N fertilizer showed a positive correlation. 6. The outbreak percentage of diseased seedlings on Pinus densiflora and Larix kaempferi was a negative trend according to their age.

Cheek Flap을 이용한 안면부 재건

한예식,홍인식,이정윤,안희창 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.4

There are several technique for facial reconstruction using local, regional and distant flaps. Most of these techniques require multiple stages, leave poor color match, and create serious complications at donor site. Since 1959, a wide variety of defects in the eyelid and cheek have been reconstructed with Mustarde's cheek flap. This can mobilize a large area to be rotated into the defect, and effectively cover a relatively large defect with minimal distortion and with good color match. Advantage of the cheek flap include easy accessibility and operative technique. In the old age group that present most commonly with eyelid malignancies, there is sufficient laxity of the skin, and therefore, abundant tissue is available to reconstruct the lower lid. Problems which can develop with this flap include hematoma, infection, or flap necrosis early in the postoperative course. Late complications include problems resulting mainly from skin tension and gravity, causing ectropion or lateral canthal distortion. The author experienced excellent functional and esthetic result form 15 cases of facial reconstruction with Mustsarde's cheek flap. We reviewed history and surgical techniques of this flap referring the literature.

초등실과 전기기구 다루기 단원의 CD-ROM 개발과 수업에의 적용 효과

윤정한,김동연,김진수 韓國實科敎育硏究學會 2001 實科敎育硏究 Vol.7 No.1

The purposes of this study was to develop CD-ROM on manipulating the electric appliances unit for the fourth grade students in elementary practical arts curriculum, and to verify the effect of it for helping students acquire the basic knowledge of manipulating the electric appliances and teachers improve teaching method. The developed CD-ROM title had the following features. 1.The learning content of this CD-ROM title was consisted around manipulating the electric appliances unit for the fourth grade students in elementary practical arts curriculum. 2.This CD-ROM title was developed using various graphics, photos, movable image and animation to motivate elementary school students and to raise the effect of learning. 3.The learning content of this CD-ROM title was consisted around the fourth grad practical arts text in elementary school to utilize this CD-ROM title in class effectively. 4.This CD-ROM title was developed with authoring tool, Tool Book 5.0 of Asymetrix. 5.The basic learning topic of this CD-ROM title was composed of five specific topics for consideration of learner's interest. The results of application of this CD-ROM title were as follows: 1.There was a meaningful difference(p<0.05) between experimental group and control group on the effect of achievement. 2.There was a meaningful difference in girl students, but not a meaningful difference in boy students on the effect of achievement.

초·중·고등학교 학생·학부모·일반교사의 보건교육 영역별 요구도와 보건교육

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2005 韓國學校保健學會誌 Vol.18 No.1

Purpose: The purpose of this study was to identify the needs of health education in students, their parents and teachers in the elementary, middle and high schools and the current situation of health education class. Method: The subjects of this study were a total of 9450 persons including students, their parents and teachers from 279 schools throughout the country. They were selected through convenient sampling. Data were analyzed through x^(2)­test and ANOVA. Result: Students, their parents and teachers replied that 18 dimensions of health education class (DHEC) are necessary. The four DHEC - healthier life style, sex education, mental health and safety education - showed high educational needs in students, their parents, and teacher. High school students had higher educational need of 'symptom management for daily living' than elementary and middle school students. Students, their parents and teachers in elementary school had higher educational needs of 17 DHEC that those in middle and high school. The percentages of schools with health education class taught by health teachers were 99.2%, 75.5% and 66.0% respectively in elementary, middle and high schools. Health education was given mainly using physical education classes at elementary schools, and creative class hours at middle and high schools. In general, health education took 1-3 hours per week at elementary schools, and less than an hour at middle and high schools. Conclusion: Therefore, based on the results, systematic health education class should begin from elementary school to meet the need of health education in students, their parents and teachers, and further study should be made on the number of hours required and the amount of contents of 18 DHEC.

Purification and Characterization of Peroxidase from Streptomyces sp. ND002

한윤전,정병철 명지대학교 자연과학연구소 2002 자연과학논문집 Vol.21 No.-

본 실험은 아조계 염료인 congo-red에 대한 분해능이 밝혀져 있는 Streptomyces sp. ND002균주에서 peroxidase를 분리·정제하여 그 역할을 규명하는데 목적을 두고 수행되었다. Streptomyces sp. ND002 균주를 pH 7.0의 조건에서 DJMM배지를 사용하여 배양을 실시한 결과 최고 5Units/ml의 세포 외 peroxidase 활성을 나타내었다. 균주 배양액을 이용하여 Q sepharose FF anion exchange column, Superdex 200 pg gel filtration column, Phenyl sepharose HP hydrophobic interaction column, Mono Q HIR anion exchange column chromatography를 통하여 최종적으로 speecific activity가 68.63 U/mg protein인 peroxidase를 부분 정제하였다. 정제과정 중 perosidase 활성이 분리되어 나타나는 것으로 보아 streptomyces sp. ND002 균주는 최소 3종류 이상의 2,4-DCP 분해능을 지니는 peroxidase를 세포 외로 분비하는 것으로 보이며, 부분 정제된 peroxidase에 대한 특성 파악 결과 pH7.5와 30℃의 온도에서 활성이 높게 나타남을 확인할 수 있었고, 중성이상의 pH와 4℃에서 보존성이 높다는 결과를 얻었다. 또한 분리과정 중 peroxidase 활성을 나타내는 fraction에서 색을 뛴다는 사실을 통해 wave scan한 결과 400nm의 파장에서 높은 흡광도를 나타내며, heme protein inhilbitor인 NaN_3, KCN를 넣어주고 활성을 측정한 결과 NaN3의 경우 98%, KCN의 경우 54%의 활성의 감소가 확인되었다. 이상의 결과로 미루어 보아 현재 부분 정제된 peroxidase 는 기존의 Streptomyces viridosporus T7A에서 분리된 ALiP-P3와 같은 특징을 지니는 heme-peroxidase로 사료된다. Streptomyces sp. ND002 is a gram-positive soil actinomycetes secreting an uncharacterized 2,4-dichlorophenol (DCP) oxidizing enzyme, whose activity is similar to the previously know Actinomycetes Lignin Peroxidase (ALiP). This extracellular peroxidase was purified from Streptomyces sp. ND002 by Q-sepharose, Superdex 200pg, Phenyl sepharose HP, Mono Q HIR column chromatography. The highest level of peroxidase activity was observed at pH7.5 and 30℃. The purified peroxidase was inhibited by 1 mM sodium azide and potassium cyanide treatment. Additionally, purified peroxidase exhibited and absorption spectrum at 400 nm. Thus, these results and observations suggest that this perxidase is heme protein, like ALiP-P3.

국내산 무화과를 이용한 와인 제조 및 품질 개선 효과

정미란,차정단,윤순일,한종현,이영은 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.1

Fig(Ficus carica L.) which belongs to Moraceae family has been used as digestion promoter, cure for ulcerative inflammation and eruption in Korea. Figs have very short preservation period and can be kept only 1 week even in the refrigeration system. The possibility of wine-making from Korean figs was tested by analyzing the fermentation characteristics, proximate analysis and free sugar contents of wine made of slices with 20% sugar addition. Ethanol fermentation of the figs by Saccharomyces cerevisiae was done rapidly. The ethanol contents, pH, total acidity and sugar content were 12.1%, 3.91, 0.42% and 9.9 °Brix, respectively. The results from the sensory evaluation were as follows. The wine made from the sliced figs were better than that from fig juices in their sensory scores. The wine made with frozen figs was not different from that made with fresh ones. To improve the quality of wine, the addition of dried leaf powder in the concentration of 100 ppm were tried and found it was superior to the one made without leaf.

철근콘크리트 연속보에서 부분프리스트레스 도입에 의한 휨보강 효과에 관한 연구

한병찬,윤현도,박완신,황선경,임재형,문정호 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

A strengthening technique for reinforced concrete beams using external unbonded reinforcement offers advantages in speed and simplicity of installation over other, established, strengthening techniques. The purpose of this paper is to investigate the capabilities of a new retrofitting technique, namely external prestressing(Bow), for flexural strengthening of beams. The paper provides a general description of structural behavior of beams strengthened using the technique. Results of 2 physical tests on strengthened reinforced concrete continuous beams are reported and compared. It is shown that the technique can provide greater strength enhancement to lightly reinforced sections and that provision of deflectors enhances efficiency.

초·중·고등학교 학생, 학부모, 일반교사의 보건교육 요구

윤순녕,김영임,최정명,조희순,김영희,박영남,오경순,이분옥,조선녀,조소영,한선희,하영미 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.2

Purpose : The purpose of this study was to identify needs of health education of the students, parents, general teachers, and health teacher of the elementary, middle, and high schools. Method : 279 schools of elementary, middle, and high school were sampled nonproportionally during the period from June 28 to July 26, 2004 . The data were collected by the questionnaire from 1,888 students, 1,876 parents, 1,695 general teachers, 279 health teachers. A frequency, % and χ2-test were used to analysis by SAS program. Result : 1. It showed that 98.1% of elementary school students, 92.3% of middle school students, 89.6% of high school students answered that they need health education. The most of students, parents, and general teachers had high interests in health education. 2. In the case of students, main causes of needs of health education was 'to prevent diseases or accidents''. But parents answered that it was 'to build up a healthier behavior'. General teachers answered that it was 'more effective systematic and continuous health education'. 3. Over 80% of students, parents, and general teachers about the question of who qualified person is to teach health education as a regular class responded that health teacher is available. Especially 93% of elementary school students answered like that. 4. The most of students, parents, and general teachers answered that health education in the classroom is favorable 1 to 2 hours per week. Conclusion : As results of the study , the majority of students, parents, general teachers agreed needs of health education. Therefore, on the basis of this study, systematic and continuous health education is necessary. Additionally the establishment of independent health subject is required certainly.

과도 절삭에서의 절삭 공정 시뮬레이션

고정훈,조동우,윤원수,김주한 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

In most of the existing mechanistic models, the cutting process simulation is often restricted to a single path machining operation under a fixed cutting condition. Complex cutting processes such as die or mold manufacturing, however, are performed under two- or three-dimensional multiple tool paths. Since the tool paths in CNC machining are composed of line and arc segments, transient cuts are frequently occured due to the multiple paths. Even in steady cuts, the width of cut is varied with each segment. In this regard, this paper deals with the development of process simulation system for transient cuts, where continuously changing cutting configuration is computed, and then the cutting forces are predicted.