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( Yoonjee Park ),( Darae Kim ),( Jeong Hoon Yang ),( Yang Hyun Cho ),( Jin-oh Choi ),( Eun-seok Jeon ) 대한내과학회 2022 The Korean Journal of Internal Medicine Vol.37 No.2
Background/Aims: The continuous flow left ventricular assist device (cf-LVAD) has improved the survival of chronic end-stage heart failure (HF) patients. Here we describe our clinical experience of the initial 50 LVAD patients from a single center. Methods: A total of 50 patients underwent LVAD implantation as bridge to transplantation (BTT; n = 28, 56%), bridge to candidacy (BTC; n = 2, 4%), or as destination therapy (DT; n = 20, 40%) from 2012 to 2019. Pre-implant characteristics and clinical outcomes were compared between BTT/BTC and DT. Results: The median age of patients was 67 years (range, 59 to 73). Men were more likely to receive LVAD (76% vs. 24%) than women. DT patients were older, had smaller body surface area, and worse laboratory profiles than BTT/BTC patients. There was no in-hospital mortality. During an average of 14 months (range, 8 to 23), the all-cause mortality was 22%. The first-year survival was 86 and 90% in BTT/BTC and DT groups, respectively. Hemorrhagic stroke was the most common cause (27%) of death. In the BTT/BTC group, 22 patients successfully underwent heart transplantation during median duration of 10 months (range, 7 to 14). The most common post-LVAD complication during the first year of LVAD implantation was major bleeding (44%). A significant proportion (76%) of patients experienced rehospitalization with gastrointestinal bleeding as the most common cause. Conclusions: We describe short-term clinical outcome of LVAD patients from a single center for the first time in Korea. With the newer generation LVAD and a dedicated team approach, improved clinical outcomes of LVAD for end-stage HF are expected.
Park, Jung Ha,Park, Kyung-Min,Chang, Yoonjee,Park, Jun-Young,Han, Jaejoon,Chang, Pahn-Shick Elsevier 2018 Enzyme and microbial technology Vol.119 No.-
<P><B>Abstract</B></P> <P>In this study, the gene of a novel lipase with <I>sn</I>-1(3) regioselectivity (<I>i</I>.<I>e</I>., <I>sn</I>-1 or <I>sn</I>-3 specific) from <I>Cordyceps militaris</I> was successfully expressed by a heterologous expression system. Total RNA was extracted from <I>C. militaris</I> and then single-stranded cDNA was synthesized. The resulting <I>C. militaris</I> lipase (<I>CML</I>) gene was inserted in <I>Escherichia coli</I> expression plasmids [pET-29b(+), pET-26b, and pColdIII] to construct plasmids encoding <I>CML</I>, which were then transformed to <I>E. coli</I> strains BL21 (DE3), C43 (DE), C41 (DE3), and Origami (DE3) for protein expression. Although the recombinant <I>CML</I> expression level was high, it was overproduced in the form of inclusion bodies. Under a specific condition, the soluble form of the recombinant <I>CML</I> was detected using Western blot analysis; however, no enzyme activity was observed. To overcome the lack of post-translational modifications in recombinant <I>CML</I>, a baculovirus-insect expression system was introduced for eukaryotic lipase expression. pDualBac was used as the transfer vector, and the <I>CML</I> gene was fused under the control of the polyhedrin promoter. After generating the recombinant baculovirus, the active form of <I>CML</I> was successfully produced and its kinetic parameters were determined.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The gene of <I>Cordyceps militaris</I> lipase (<I>CML</I>) was cloned in the microbial systems. </LI> <LI> Total RNA was extracted from <I>C. militaris</I>, and the cDNA of <I>CML</I> was synthesized. </LI> <LI> In baculovirus-insect expression system, the <I>CML</I> was expressed as active forms. </LI> <LI> Kinetic parameters were determined to characterize the recombinant <I>CML</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
S-239 Hs-CRP Predicts New-Onset HF in Patients Treated with Statin for Primary Prevention
( Yoonjee Park ),( Byoung-geol Choi ),( Seung-woon Rha ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Background: The inflammatory biomarker high-sensitivity C-reactive protein (hsCRP) is known to be associated with the progression and worsening of heart failure (HF). We evaluated the impact of hsCRP on the development of new onset HF in patients (pts) taking moderate-intensity statin for primary prevention.?Methods: Pts without HF(N-terminal pro-B-type natriuretic peptide (NT-proBNP) > 400 pg/ml) were enrolled and divided into 4 quartiles according to hsCRP levels. New-onset HF as primary endpoint and other cardiovascular events as secondary endpoints were evaluated up to 9 years.?Results: Compared to the lowest quartile (<0.46 mg/L, n=1224), the highest quartile (>1.86 mg/L, n=1194) of hsCRP was associated with a higher incidence of new-onset HF [OR= 2.28, (95% CI 1.63-3.20), p<0.001]. Even after proportional hazard cox-regression adjusted by age, gender, cardiovascular comorbidities including arrhythmia, hypertension, diabetes, chronic kidney disease, laboratory parameters and medications including types of statins, there was a weaker but still significant relationship between higher levels of hsCRP and the development of new-onset HF [HR= 1.74, (95% CI 1.23-2.46), p=0.002, figure]. Conclusions: In our study, hsCRP levels remaining high even after moderate-intensity statin treatment significantly predicted the development of new-onset HF during long-term follow-up. Risk stratification according to the level of hsCRP may help identify pts who need additional attention for future deterioration of cardiac function.
알루미늄과 마그네슘 첨가가 용융아연 도금강판 도금층의 미세조직과 경도에 미치는 영향
성윤제 ( Yoonje Sung ),김동규 ( Donggyu Kim ),서준기 ( Jungi Seo ),한경현 ( Kyunghyun Han ),홍범기 ( Beomki Hong ),김강민 ( Kangmin Kim ),허성욱 ( Seounguk Heo ),박성현 ( Seonghyun Park ),임재택 ( Jae-taek Im ),손승배 ( Seung Bae 한국열처리공학회 2023 熱處理工學會誌 Vol.36 No.4
We investigated the effects of Al and Mg on the microstructure and hardness of the coating layer of galvanized steel sheets, by thermodynamic calculations, X-ray diffraction, scanning electron microscopy, and Vickers hardness tests of Zn-0.2Al, Zn-6Al-2Mg, and Zn-10Al-5Mg coating layers. Regardless of the alloy composition of the galvanizing bath, a Fe-Al layer was observed between the coating layer and steel sheet. The Zn- 0.2Al coating layer consists of major h.c.p. Zn phase and minor f.c.c. Al phase. The fraction of f.c.c. Al phase (containing a significant amount of Zn) of the coating layer increases with increasing the chemical composition of Al of the galvanizing bath. The h.c.p. MgZn<sub>2</sub> phase was formed in the Al/Mg-containing Zn-6Al-2Mg and Zn-10Al- 5Mg coating layers, forming Zn-Al-MgZn<sub>2</sub> eutectic microstructure. The primary MgZn<sub>2</sub> phase was additionally formed in the Zn-10Al-5Mg coating layers containing high concentrations of Al and Mg. The Vickers hardness values of Zn-0.2Al, Zn-6Al-2Mg, and Zn-10Al-5Mg coating layers were 59.1 ± 1.2 HV, 161.2 ± 5.7 HV, and 215.5 ± 40.3 HV, respectively. The addition of Al and Mg increased the hardness of the coating layer by increasing the fraction of the Al phase (containing Zn) and MgZn<sub>2</sub> intermetallic compound, which were harder than the Zn phase. (Received June 7, 2023; Revised July 11, 2023; Accepted July 20, 2023)
Palmarosa oil-loaded nanoemulsion as a potential biocontrol agent to suppress soft rot
Ji-Hwa Park,Huihyun Shin,Sangbin Kim,Yoonjee Chang,Jee-Young Imm 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The potential of essential oils to suppress soft rot of agricultural products was evaluated and palmarosa oil (Cymbopogon martinii) was selected out of 7 edible essential oils based on minimum inhibitory concentration (MIC) against Pectobacterium carotovorum and sensory impact. Palmarosa oil was formulated as water-dispersible naonoemulsion (PNE) with 1:2 ratio (v/v) of oil and tween 80 using high-intensity ultrasound. PNE was stable with mean diameter of 46 nm and the zeta potential of –13 mV. The particle size of PNE was not significantly changed even after refrigerated storage for 1 month. MIC of PNE was 600 ppm and PNE killed almost all the bacteria in about 10 min in microbial deactivation kinetic assay. Antioxidant activity determined by TBHP-induced intracellular ROS accumulation in HepG2 hepatoma cells was significantly enhanced by conversion of palmarosa oil into a nanoemulsion. Based on the results, PNE can be developed as edible coating ingredient to control postharvest decay such as soft rot.