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( Lixiaoxia ),노경호 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
Four kinds of new type of green betaine-based deep eutectic solvents (DESs) have been prepared in this study. And four betaine-based mesoporous materials and conventional mesoporous materials (no DESs) have been synthesized by hydrothermal synthesis. These mesoporous materials were applied to HPSEC column packing. In addition, the six different kinds of dextran (MW: 5, 25, 50, 270, 670 and 1100 KDa) with the different molecular weight have been applied in the three times injection testing as the target compounds. DESs based mesoporous materials shown the better separation effect than conventional mesoporous materials. However, betaine-urea based column was selected as the best one. It had a good separate effect to molecule lower than 270K. All of these results indicated that betaine-based DESs were involved in the synthesis of materials, and they had good effect on the separation effect. Their separation efficiencies were examined according to their mesoporous structures.
( Lixiaoxia ),( Xiaoqin Wang ),노경호 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.0
Mesoporous materials were prepared in choline chloride deep eutectic solvents by triblock copolymer-templated hydrothermal synthesis. The mesoporous material-based choline chloride-ethylene glycol (1:2 mole ratio) DES had a flower-type shape, but the material-based choline chloride-urea (1:2) and based choline chloride-acetic acid (1:2) had mesoporous spherical shapes. These mesoporous materials were applied to HPSEC column packing. The separation effect of the two dextrans was better from the choline chloride-acetic acid deep eutectic solvent-based mesoporous spheres packed in a high-performance SEC column. In addition, the three polysaccharides, dextran-1 (molecular weight: 670 KD), dextran-2 (molecular weight: 50 KD) and dextran-3 (molecular weight: 5 KD), were separated successfully using the choline chloride-acetic acid deep eutectic solvent-based mesoporous spheres packed in a high-performance size exclusion chromatography column.
( Lixiaoxia ),( Tangweiyang ),노경호 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
New types of two-dimensional boron nitride were developed as a 2D scaffold material. After modification by deep eutectic solvents, the BN was applied to the preparation of MIP. The proposed two dimension material had been first applied in preparation of molecularly imprinted polymers as a novel monomer. The MIP for the preconcentration flavonoids from Ginkgo Biloba leaves extractive. The elution conditions of solid-phase extraction method had optimized by computational models and methodology. Two dimension MIP was synthesized and characterized using fourier transform infrared, thermal gravimetric analysis, scanning electron microscopy, and nitrogen sorption analysis techniques. The proposed materials had a two-dimensional structure and large surface area. HPLC-UV analysis results revealed the applicability of molecularly imprinted polymers in solid phase extraction and determination of melamine from food samples.
Application of Intracytoplasmic Sperm Injection in Mammals
Li, Xiao Xia,Lee, Kyung Bon,Lee, Ji Hye,Kim, Keun Jung,Park, Kang Sun,Kim, Min Kyu The Korean Society of Animal Reproduction 2013 Reproductive & developmental biology Vol.37 No.2
For more than two decades, the intracytoplasmic sperm injection (ICSI) technique has been used as a valuable tool to provide opportunities for studying fertilization, treating human infertility, and producing transgenic animals. Not only in facilitating fertilization but also in propagating mammalian species, ICSI has enhanced the potential of assisted reproductive technologies in human. Polyspermic fertilization has been one of major problems in pig reproduction, but the ICSI helped to solve the problem, and used widely to generate transgenic piglets. Although the ICSI technique is considered to be a very useful tool in assisted reproductive technologies, including generation of transgenic animals, there are some disadvantages using the technique. In this review, we describe the ICSI technique and its application in animal production and human infertility, and discuss advantage and disadvantage of the technique in mammals.
Li, Xiao-Xia,Zhang, Yong-Gang,Wang, Dong,Chen, Yun-Fang,Shan, Yan-Hua Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12
Objective: To explore the preventive effect of aspirin on the cardiovascular complications in prostate cancer after endocrinotherapy. Materials and Methods: A total of 92 patients with prostate cancer were divided into observation group (n=44) and control group (n=48). The control group was treated with medical castration plus anti-androgenic drugs. Based on the above treatment, the observation group was added aspirin. The follow-up duration was 2 years. The changes of partial prothrombin time (PT), activated partial thromboplastin time (APTT), platelet aggregation rate (PAG), prostate-specific antigen (PSA) and serum testosterone (T) before and after treatment as well as incidence of cardiovascular disease were observed. Results: The 2-year survival rates of patients without cardiovascular disease in observation group and control group were 95.45% (42/44) and 72.92% (35/48), respectively, and significant difference was presented between two groups by comparison to the survival rates ($x^2=8.5453$, p=0.0035). There was no statistical significance between two groups as well as before and after treatment regarding PT (p>0.05). After treatment, APTT went down and PAG was gradually on the rise in control group, while PAG down and APTT on the rise increasingly in observation group. Significant differences were presented between two groups as well as before and after treatment (p<0.01). Both PSA and T levels were decreased significantly in two groups after treatment (p<0.01), but there was no statistical significant between two groups (p>0.05). Conclusions: Application of endocrinotherapy in prostate cancer can easily lead to occurrence of cardiovascular disease, but cardiovascular complications can be prevented by aspirin, without affecting the effect of endocrinotherapy.
Li, Xiaoxia,Row, Kyung Ho Elsevier 2017 Journal of chromatography. B, Analytical technolog Vol.1068 No.-
<P><B>Abstract</B></P> <P>A series of ecofriendly ternary deep eutectic solvents (DESs) with different molar ratios were prepared as candidate functional monomers. Three of the optimal ternary DESs as functional monomers were applied to the preparation of molecularly imprinted polymers (MIPs). After synthesis, the proposed polymers were characterized by elemental analysis (EA), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Brunauer-Emmett-Teller surface area measurements (BET) and Fourier transform infrared spectroscopy (FT-IR). These MIPs based on ternary DESs with different molar ratios exhibited different absorption capacities of levofloxacin. A sample of levofloxacin (500ng) was dissolved in a millet extractive (10mL). All MIPs were used as SPE adsorbents to purify the extracts. According to characterization result, the ternary DES-3 (1:3:1.5) was joined in the synthetic process of MIP-1. The green ternary DES-3-based MIPs had the best selectivity recovery for levofloxacin (91.4%) from the millet extractive. The best selectivity of MIP-1 was attributed to the novel monomer (ternary DES) in the preparation of the materials. Overall, ternary DES-based MIPs have potential applications as media in many research areas.</P>
Li, Xiaoxia,Dai, Yunliang,Row, Kyung Ho The Royal Society of Chemistry 2019 The Analyst Vol.144 No.5
<P>New types of two-dimensional (2D) boron nitride (BN) were developed as a 2D scaffold material. After modification with ternary deep eutectic solvents (DES, ChCl-caffeic acid-ethylene glycol), the processed BN was applied to the preparation of magnetic molecularly imprinted polymers (MMIPs). In the polymerization of MMIPs, DESs and hydrophobic Fe3O4 magnetite were applied as the functional monomer and magnetically susceptible component, respectively. A 2D ellipsoid material was formed successfully by polymerization on the surface of the processed BN. The proposed 2D-MMIPs were characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy, and nitrogen sorption analysis techniques. The surface area of the 2D-MMIPs was increased using eco-friendly chemicals. The proposed 2D-MMIPs had a 2D oblate structure and a large surface area. The 2D-MMIPs were used for the preconcentration of flavonoids from <I>Ginkgo biloba</I> leaf extracts. High performance liquid chromatography-ultraviolet analysis revealed that the 2D-MMIPs have higher recoveries for the flavonoids (quercetin 96.8%, isorhamnetin 93.6% and kaempferol 94.8%) in <I>Ginkgo biloba</I> leaves than common MMIPs.</P>