표고골목 해균인 주홍꼬리버섯을 방제하기 위한 저항성 표고균주 선발

이봉훈,박원철,가강현,윤갑희,박현,차병진 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: ① continuous incubation at 14℃, ② continuous incubation at 25℃, ③ changing of incubation temperature from 14℃ to 25 ℃ as soon as mycelia of both shiitake and D. stigma meet together, ④ changing of incubation temperature from 25℃ to 14 ℃ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: ① inoculation of shiitake 3 days ahead of D. stigma inoculation, ② inoculation of D. stigma 3 days ahead of shiitake inoculation, ③ simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from 14℃ to 25℃ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at 25℃ decreased when the temperature was changed into 14℃ after mycelia of both fungi. In these cases, the resistance reached to 7∼20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at 25℃.

표고골목 해균인 주홍꼬리버섯을 방제하기 위한 저항성 표고균주 선발

이봉훈 외 한국버섯학회 2005 한국버섯학회지 Vol.3 No.3

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: ① continuous incubation at 14℃, ② continuous incubation at 25℃, ③ changing of incubation temperature from 14℃ to 25 ℃ as soon as mycelia of both shiitake and D. stigma meet together, ④ changing of incubation temperature from 25℃ to 14 ℃ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: ① inoculation of shiitake 3 days ahead of D. stigma inoculation, ② inoculation of D. stigma 3 days ahead of shiitake inoculation, ③ simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from 14℃ to 25℃ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at 25℃ decreased when the temperature was changed into 14℃ after mycelia of both fungi. In these cases, the resistance reached to 7∼20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at 25℃. 표고골목 해균인 주홍꼬리버섯을 방제하기 위해 저항성 표고균주 선발을 시도하였다. 저항성 표고균주 선발 시험에서, 사용된 77개 균주 중 67개 균주가 저항력을 가지는 것으로 확인되었다. 이들 67개 균주 중 KFRI 5를 포함한 13개 균주가 주홍꼬리버섯균의 접근을 막는데 가장 큰 영향을 미쳤고 KFRI 180을 포함한 7개 균주가 주홍꼬리버섯의 영역으로 가장 많이 침범했다. 교잡을 통해 만든 31개 균주 중 KFRI 537을 포함한 8개 균주가 주홍꼬리버섯균의 접근을 막는데 가장 큰 영향을 미쳤고 KFRI 545를 포함한 4개 균주가 주홍꼬리버섯의 영역으로 가장 많이 침범했다. 온도와 접종순서의 변화에 따른 각 균주의 저항력 차이를 확인하기 위해 PDA 평판배지와 상수리나무톱밥을 넣은 시험관배지를 사용했다. PDA 평판배지 시험결과, 대부분의 처리구에서 KFRI 137은 주홍꼬리버섯에 대해 뛰어난 접근 억제력을 보였고 KFRI 180은 뛰어난 침범력을 보였다. 교잡균인 KFRI 545, 546, 547은 모균주들인 KFRI 488과 405에 비해 높은 저항력을 보였다. 시험관배지 시험결과, 표고균과 주홍꼬리버섯균이 대치를 하는 시점부터 25℃에 두었을 때 모든 균주가 높은 저항력을 나타냈다. KFRI 393은 25℃에서 지속 배양할 때 다른 균주에 비해 높은 저항력을 나타냈다. 또한 모든 균주들에서, 25℃ 배양 후 두 균이 대치를 할 시기에 14℃로 옮겨 배양한 처리구는 다른 처리구들에 비해 낮은 저항력을 보였다. 이 때의 저항력 정도는 각 균주 최고치의 7-20%에 불과했다. 또한 KFRI 259는 PDA 평판배지에서와 달리, 주홍꼬리버섯의 영역을 침범했다.

삼계용 신품종 토종닭의 육질특성 비교분석

이성윤(Sung-Yun Lee),박지영(Ji-Young Park),현정민(Jung-Min Hyun),정사무엘(Samooel Jung),조철훈(Cheorun Jo),남기창(Ki-Chang Nam) 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.3

기존의 삼계용 토종닭 품종과 GSP 육종을 통해 개발 중인 신품종 토종닭 후보라인의 도체 및 육질 특성을 비교하기 위해, 동일 조건에서 사육한 5주령의 한협3호(H), 백세미(W), 토종닭 후보라인 3계통(A, C, D)으로 구성된 250수를 도축하여 도체형질을 측정하고, 가슴육을 이용하여 육질성분과 특성을 분석하였다. 도체중은 W가 875.10 g으로 가장 높았고, 후보라인A가 537.54 g으로 가장 낮았다(P<0.05). W는 브로일러의 특성인 가슴살의 비율이 높고, 가슴살의 지방함량도 높았다. 후보라인 3계통은 H와 W보다 linoleic acid(C18:2)의 함량이 높았다. 특히 후보라인 중 A와 D는 토종닭에서 특징적으로 많이 함유되어 있는arachidonic acid(C20:4) 함량이 C에 비해 높았다. 전반적인 지방산 조성에서 후보라인 A와 D는 대조구 H와 유사하였으며, 후보라인 C는 W와 유사한 경향을 보여 흥미로웠다. 백세미(W) 보다는 시중 토종닭(H)과 유사한 지방산 조성을 보인 후보라인 A와 D의 관능평가 점수가 우수한 것으로 나타났다. 이 연구 결과를 바탕으로 기존의 삼계용 토종닭과 백세미를 대체하는 새로운 품질특성을 지닌 삼계용 토종닭 개발의 기초자료로 활용 가능할 것이다. The carcass and meat quality of new native chicken strains (A, C, and D) being developed in the Golden Seed Project for Samgyetang were compared with a commercial native chicken (Hanhyup No. 3, H) and Baeksemi (white semi broiler, W) strains. In total, 250 male chicks were raised under the same feeding and raising conditions for 5 weeks. After slaughter, the carcass weight and percentage yield of parts from each strain were measured and the physicochemical quality traits (general composition, color values, fatty acid composition, and sensory evaluation) of breast muscles were comparatively analyzed. The carcass weight (875.10 g) of the W strain was significantly heavier than that of the other native chicken strains, while that of A strain (537.54 g) was the lowest. The percentage yield of breast meat was also higher in the W strain than in the other strains. The fat content of W strain was higher than that of the other native chicken strains. The new native chicken strains (A, C, D) had higher contents of linoleic acid (C18:2) than the H strain. The A and D strains especially showed relatively high content of arachidonic acid (C20:4), a characteristic fatty acid in native chicken meats. On the other hand, the fatty acid composition of C strain was similar to that of the W strain. This study provides important information on specific quality characteristics than can be used to select new native chicken lines for breeding program.

Molecular identification of the strains of the domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), which are endemic to Korea, based on single nucleotide polymorphisms in mitochondrial genome sequences

Park Jeong Sun,Kim Min Jee,Kim Seong-Wan,Kim Kee-Young,Kim Seong-Ryul,김익수 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

The domestic silkworm, Bombyx mori (Lepidoptera: Bombycidae), has been diversified into various strains over a long period. However, methods to distinguish silkworm strains remain limited partially owing to the genetic similarity caused by the long history of domestication. In this study, we developed molecular identification methods to distinguish three domestic silkworm strains, which are endemic to Korea. By comparing publicly available complete mitochondrial genome (mitogenome) sequences of five endemic strains and 34 stock silk worm strains analyzed in a previous study, we detected 15 single nucleotide polymorphisms (SNPs; SNP1–SNP15), which distinguished the following three endemic strains: Sun7ho (SN7), Sandongsammyeon (SDS), and Sammyeonhonghoeback (SMH). We used two SNPs for each strain to identify the three endemic strains. To distinguish each SN7 and SDS from the remaining four endemic and 34 stock strains, the PCRrestriction fragment length polymorphism method was employed using Acu I and Hpa I restriction enzymes, which recognize SNP1 and SNP8, respectively. Additionally, the tetra-primer amplification refractory mutation system PCR method was used to determine the regions containing SNP3, SNP11, and both SNP14 and SNP15 to distinguish SN7, SDS, and SMH, respectively, from the remaining strains. A validation test with additional in dividuals showed that each target strain was clearly recognized, suggesting that mitogenome SNP-based methods can be used to identify three endemic silkworm strains during culture and breeding.

Comparative Studies on Behavioral, Cognitive and Biomolecular Profiling of ICR, C57BL/6 and Its Sub-Strains Suitable for Scopolamine-Induced Amnesic Models

Karthivashan, Govindarajan,Park, Shin-Young,Kim, Joon-Soo,Cho, Duk-Yeon,Ganesan, Palanivel,Choi, Dong-Kug MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.8

<P>Cognitive impairment and behavioral disparities are the distinctive baseline features to investigate in most animal models of neurodegenerative disease. However, neuronal complications are multifactorial and demand a suitable animal model to investigate their underlying basal mechanisms. By contrast, the numerous existing neurodegenerative studies have utilized various animal strains, leading to factual disparity. Choosing an optimal mouse strain for preliminary assessment of neuronal complications is therefore imperative. In this study, we systematically compared the behavioral, cognitive, cholinergic, and inflammatory impairments of outbred ICR and inbred C57BL/6 mice strains subject to scopolamine-induced amnesia. We then extended this study to the sub-strains C57BL/6N and C57BL/6J, where in addition to the above-mentioned parameters, their endogenous antioxidant levels and cAMP response-element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) protein expression were also evaluated. Compared with the ICR strain, the scopolamine-inflicted C57BL/6 strains exhibited a substantial reduction of spontaneous alternation and an approximately two-fold increase in inflammatory protein expression, compared to the control group. Among the sub-strains, scopolamine-treated C57BL/6N strains exhibited declined step-through latency, elevated acetylcholinesterase (AChE) activity and inflammatory protein expression, associated with reduced endogenous antioxidant levels and p-CREB/BDNF expression, compared to the control and tacrine-treated groups. This indicates that the C57BL/6N strains exhibit significantly enhanced scopolamine-induced neuronal impairment compared to the other evaluated strains.</P>

부가 탄소원이 Bacillus sp. 79-23의 변이주에서 셀룰라제의 생산성에 미치는 영향

윤기홍,신인경 우송대학교 부설 산업연구원 1999 산업연구 Vol.1 No.2

Bacillus sp. 79-23의 포자를 감마선으로 조사하여 얻은 7개 변이주의 CMCase 생산성에 미치는 탄소원의 영향을 모균주와 비교 분석하였다. LB 배지에 부가탄소원으로 glucose, xylose, maltose, lactose, CMC, starch, 볏짚, 밀기울을 첨가하여 배양한 후 배양상등액에 존재하는 CMCase의 활성을 측정한 결과 변이주의 효소생산성은 탄소원에 의해 모균주와 유사한 영향을 받는 것으로 나타났으나 변이주에 따라 약간의 차이는 존재하였다. 특히 xylose와 볏짚에 의한 변이주들의 효소생산량간에 차이가 나타났다. 대부분 변이주의 성장정도도 모 균주와 유사한 것으로 확인되었으며 효서는 성장과 연계되어 생산되었다. 그러나 변이주 48에 잇어서 탄소원에 의한 효소 생산성의 영향은 유사하지만 모균주나 다른 변이주에 비해 성장정도가 매우 낮으며 효소 생산량도 다른 균주에 비해 낮은 것으로 밝혀졌다. 이로보아 변이주에서의 유전자 변이는 서로 다른 지역에서 일어난 것으로 생각된다. 한편 CMCase를 코드하는 유전자를 포함한 부위가 모든 변이주와 모균주의 총 유전체 DNA로부터 동일한 primers로 증폭되었으며 그 크기가 전기영동상에서 동일한 것으로 확인되었다. 그러므로 감마선 조사에 의해 얻어진 변이주는 CMCase 유전자 부위에 삽입이나 결손등의 변이가 일어나지 않은 것으로 판단된다. In seven mutant strains which were derived from Bacillus sp. 79-23 by gamma-irradiation of the parental spores, effects of carbon sources were investigated on carboxymethylcellulase (CMCase) production. By measuring the CMCase activity in the supernatants of bacterial cultures which were grown in LB medium containing glucose, xylose, maltose, lactose, carboxymethylcellulose, strach, rice straw, and wheat bran as additional carbon sources, it was identified that seven mutants were comparable to the parent strain. Among the additional carbon sources, xylose and reice straw affected differently the CMCase production according to the mutant stains. It was found that the enzyme production was in association with the growth of parent and mutant strains. Growth of mutant strains reached the level of parent strains. Growth of mutant strains reached the level of parent strain except mutant 47. Mutant strain 48 was poorly grown and produced CMCase less than other mutant strains. As the results of them, it was suggested that mutagenesis of the mutant strains occurred in different positions of their chromosomal DNAs, respectively. DNA fragments with an identical molecular size of 1.6 kb corresponding to the gene encording a Bacillus sp. 79-23 CMCase were amplified from genomic DNAs of all mutants and parent strain using same primers by polymerase chain reaction, proposing that mutations did not be cuased by the insertion and deletion in the structural CMcase genes of the seven mutants.

국내 분포 보리호위축바이러스(Barley Yellow Mosaic Virus) strain에 대한 저항성 유전자 반응

박종철,노태환,박철수,강천식,강미형,이은숙,이준희,이정준,김태수 한국식물병리학회 2009 식물병연구 Vol.15 No.2

It was investigated the responses of BaYMV resistant genes to Korean BaYMV(Barley yellow mosaic virus) strains. BaYMV was distributed dominantly with about 51% detection ratio among the three investigated virus such as BaYMV, BaMMV(Barley mild mosaic virus) and SBWMV(Soil-borne wheat mosaic virus) in ELISA test. Double infection with BaYMV and BaMMV was detected also higher as 38.8%, however, BaMMV sole infection ratio was lower with only 1.4%. The 11 BaYMV resistant genes were tested their responses to four Korean BaYMV strains, BaYMV-N, H, I and M. Generally, rym 3 genes showed resistant to Korean BaYMV strains and rym 4m and 5a also was better. Three genes, rym 1+5(Mokusekko-3), rym 3(Ea 52, Baitori) and rym 5a(Solan) showed resistant responses to BaYMV-N type. In -H strain test, seven genes that rym 2(Mihori Hadaka 3), rym 3(Ea 52, Haganemugi, Baitori), rym 4m(Diana, Franka), rym 5a(Solan), rym 7(Hor 3365), rym 9(Bulgarian 347), rym 12(Jochiwon Covered 2) were considered as resistant. The three genes that rym 1+5, rym 3 and rym 5a was effective to -I strain, and rym 3, rym 4m and rym 5a showed resistant to -M strain. It was investigated the responses of BaYMV resistant genes to Korean BaYMV(Barley yellow mosaic virus) strains. BaYMV was distributed dominantly with about 51% detection ratio among the three investigated virus such as BaYMV, BaMMV(Barley mild mosaic virus) and SBWMV(Soil-borne wheat mosaic virus) in ELISA test. Double infection with BaYMV and BaMMV was detected also higher as 38.8%, however, BaMMV sole infection ratio was lower with only 1.4%. The 11 BaYMV resistant genes were tested their responses to four Korean BaYMV strains, BaYMV-N, H, I and M. Generally, rym 3 genes showed resistant to Korean BaYMV strains and rym 4m and 5a also was better. Three genes, rym 1+5(Mokusekko-3), rym 3(Ea 52, Baitori) and rym 5a(Solan) showed resistant responses to BaYMV-N type. In -H strain test, seven genes that rym 2(Mihori Hadaka 3), rym 3(Ea 52, Haganemugi, Baitori), rym 4m(Diana, Franka), rym 5a(Solan), rym 7(Hor 3365), rym 9(Bulgarian 347), rym 12(Jochiwon Covered 2) were considered as resistant. The three genes that rym 1+5, rym 3 and rym 5a was effective to -I strain, and rym 3, rym 4m and rym 5a showed resistant to -M strain.

동위효소 및 RAPD분석에 의한 한국재래종 누에계통의 계통학적 특성

이재만 ( Lee Jae Man ),노시갑 ( Nho Si Kab ) 한국잠사학회 2001 한국잠사곤충학회지 Vol.43 No.2

집누에는 지리적인 특징에 의해 중국종, 일본종, 유럽종, 열대종 및 한국종으로 분류된다. 한국종계통은 품종수도 적을 뿐만 아니라 관련 연구도 미미한 실정이다. 본 연구는 한국재래종으로 추측되는 품종들을 국내 ? 외로부터 수집하여 동위효소 및 체액단백지유전자와 RAPD 다형분석을 실시하여 한국재래종 계통의 품종적 유연관계와 계통특성을 밝히고자 하였다. 1. 동위효소유전자의 분석결과, 몇 개의 유전자군에서 한국재래종과 타 지역종간에 유전자형 및 유전자 빈도의 차이가 명확히 나타났다. 2. RAPD의 결과를 UPGMA법에 의해 분석한 결과, 집누에군과 멧누에군으로 크게 구분되었으며 유전적 유사계수 0.6930을 기준으로 한국재래종, 일본종 및 중국종으로 그룹화되었다. 3. 이상의 결과를 종합하면, 한국재래종 누에계통은 하나의 지역종 원종계통으로 분류될 수 있는 명확한 유전적 특성을 가지는 것은 물론 한국종의 계통특성도 뚜렷한 것으로 확인되었다. This study was conducted to elucidate phylogenetic relationships and genetic characterization of silkworms that might be recohnized as the Korean native strains. Genetic characterization in isozymes and the proteins of larval hemolymph of 17 silkworms were observed by acrylamide gel eletrophoresis, on 12 genes; Bph, Bes, Ies, Amy-hc, Ict-A, -B,-D, -E -H,Pfl, Pst, Lp. Gene frequencies in each locus were compared other geographic strains. Korean native strains were remarkably different from others considered as the genetic characterization of Korean native strains. Phylogenetic relationships in Korean native strains were analysed using RAPD-PCR markers. A total of 40 primers were used and 346 bands of amplified DNA were generated from geographic strains, Genetic similarity based on the RAPD bands was used to construct phylogenetic dendrogram based on analysis of band sharing data of amplified markers. Genetic similarity ranged from 0.595 to 0.860. In the genetic relationship based on dendrogram, they were classified into Bombyxmori group (including 16 domesticated silkworm strains) and B. mandarina group. The Bombyx mori group was separated into three sub-groups at the genetic similarity of 0.6930, including Korean, Japanese and Chinese groups. According to this result, the Korean native variety can be considered as a clearly different variety from other geographic strains. It may be concluded that the Korean native strains are also one of original geographic rariety such as Japanese, Chinese, etc.

Cultural characteristics (VIII) of hybrid strains of Lentinula edodes on sawdust media

Youngae Park,Sung-I Woo,Yeongseon Jang,Rhim Ryoo,Kang-Hyeon Ka 한국버섯학회 2018 버섯 Vol.22 No.1

The nine parental strains of Lentinula edodes including cultivar “Sanbaeghyang” were selected to develop new cultivar of Lentinula edodes with good cultural characteristics and high productivity. A total of 44 strains were obtained by mono-mono hybridization. We investigated their cultural characteristics and fruiting body productivity using sawdust media cultivation method. For the cultivation, rectangular shape polypropylene bags filled with 2 kg sawdust media containing 80% of Querqus spp. sawdust and 20% of wheat bran were used. The average weight loss of sawdust media of 44 strains was 9.7% after 100 days of incubation. Nineteen strains (43%) showed above-average rates of weight loss. NIFoS 3515 had the highest weight loss rate (12.7%) among the hybrid strains, followed by NIFoS 3530 (12.4%) and NIFoS 3519 (12.3%). Overall, fruiting body production was low. After the second flush of fruiting body production, the average weight of mushrooms was 40.8 g per 2 kg sawdust medium, because fruit bodies were formed by 17 strains (38.6%) out of 44 strains. Ten strains (22.7%) showed above-average productivity. The highest productivity was shown by NIFoS 3533 (417 g), followed by NIFoS 3512 (237 g), and NIFoS 3516(211 g).

보문 : 발색반응 분석법을 이용한 표고 교배균주의 세포외효소 분비 능력 평가

권혁우 ( Hyuk Woo Kwon ),김준영 ( Jun Young Kim ),고한규 ( Han Gyu Ko ),박흥수 ( Heung Soo Park ),김성환 ( Seong Hwan Kim ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.2

Shiitake breeding requires the procedures of mating of two different parental strains and selection of hybrid strains that have good traits for the mushroom production. In this study, we tested the possibility of the use of chromogenic plate-based assay for extracellular enzyme production in order to assess and find good biochemical properties-possessed hybrid strains that were generated from genetic cross of the monokaryotic strains derived from two different parental strains of Lentinula edodes Sanjo-101ho and Sanjo-108ho. We observed that there was difference in the ability of producing β-glucosidase, avicelase, CM-cellulase, amylase, pectinase, xylanase, and protease among the monokaryotic strains. We could also comparatively assess that the ability of the seven extracellular enzymes production in the hybrid strains depended on the mating combination of the monokaryotic strains. Our results demonstrate that the assessment method for extracellular enzyme production using chromogenic plate assay could be usefully applied to the assessment of the hybrid strains derived from the breeding procedure of L. edodes.