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        Trichodina hokkaidoensis (Ciliophora: Peritrichia) isolated from olive flounder (Paralichthys olivaceus) in Korea

        조한슬,남우화,김정호 한국어병학회 2022 한국어병학회지 Vol.35 No.2

        We described Trichodina hokkaidoensis Mizuno, Matsuda, Nishikawa and Ito, 2022 from olive flounder Paralichthys olivaceus by morphological observation and molecular analysis. Morphological parameters of our specimen from 4 different sampling sites (Gangneung, Jeju, Wando, Taean) mostly coincided with those of T. hokkaidoensis. Some morphometric parameters of this trichodinid showed some inconsistency, depending on the sampling locations, but all of their partial small subunit ribosomal DNA sequences (1,182 bp) showed 100% homology with T. hokkaidoensis, originally described from artificially reared juvenile barfin flounder Verasper moseri from Japan. T. hokkaidoensis is known to cause epidermal damages to the host fish. However, there was no considerable pathological lesions in the olive flounder harboring T. hokkaidoensis in this study. The pathogenicity of T. hokkaidoensis against olive flounder needs to be investigated.

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        Morphological and molecular characterization of Trichodina (Ciliophora: Peritrichia) species from cultured starry flounder (Platichthys stellatus)

        조한슬,이재영,김정호 한국어병학회 2023 한국어병학회지 Vol.36 No.1

        Trichodinid ciliates were found from the skin scrapings of culture starry flounder (Platichthys stellatus) obtained from a local fisheries market in Gangneung. Skin scrapings were prepared and stained by either Giemsa solution or silver nitrate impregnation, for morphological observation. PCR analysis was also conducted to make molecular identification and phylogenetic analysis. Morphological parameters obtained from our specimens were the most similar with Trichodina hokkaidoensis of barfin flounder from Japan and also olive flounder from Korea. The partial 18S rDNA sequences of our specimen (n=7) also showed 100.0% similarity with those of barfin flounder and olive flounder. Several trichodinids are known to have a broad host specificity and this is thought to be the case for T. hokkaidoensis. Starry flounder is a strong candidate for land-based aquaculture production, as a target species for prospective aquaculture diversification and therefore pathogenic potentials of this ciliate should be considered.

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