해충관리용 유기농업자재(KEFAMA8)의 매미나방 유충에 대한 방제 효능 평가

이영돈,주진호,최선희,김혁수,안인 강원대학교 농업생명과학연구원 2023 강원 농업생명환경연구 Vol.35 No.4

The acceleration of global warming and the rise in average winter temperatures have resulted in the outbreak of pests such as gypsy moth (Lymantria dispar). In this study, we evaluated a natural plant extract for controlling the larvae of gypsy moth using immersion and foliar application methods. The eco-friendly insecticide (KEFAMA8) exhibited a 100% control efficacy when applied at ratios of 200:1 and 500:1 in the immersion method, demonstrating its insecticidal effect on gypsy moth larvae. Foliar application of KEFAMA8 also showed a 100% control rate, highlighting its excellent insecticidal efficacy in both immersion and foliar application methods. Therefore, this compound is promising as a natural insecticide for effectively managing the outbreak of pests like gypsy moths.

국내 매미나방(나비목: 태극나방과) 천적 및 매미나방 핵다각체병바이러스의 유전적 다양성 조사

황활수,이영수,이희아,최덕수,이경열 한국응용곤충학회 2021 한국응용곤충학회지 Vol.60 No.4

아시아계통의 매미나방(Lymantria dispar asiatica) (나비목: 태극나방과)은 국내 토착해충으로서 지역에 따라 돌발적으로 대발생한 사례 가 있으며, 다양한 수목 및 농작물에 피해를 끼치는 광식성 해충이다. 특히 2019년 이후로 경기도, 충청도, 경북 북부지역에서 대발생하여 산림 및 인근지역 도심에 발생하여 산림 및 도시민들의 정서적 피해를 끼치기도 했다. 본 연구에서는 2020-2021년 경북 예천지역에서 알집을 채집하여 사육한 결과, 매미나방 핵다각체병바이러스(LdMNPV) 감염에 의해 79.65% (321/403마리)는 사육중 사망하였다. 염기서열 분석은 2021년 국내 12 지역에서 매미나방 유충을 36마리를 조사한 결과, LdMNPV의 late expression factor-8 (lef-8), polyhedrin (polh) 유전자의 종내변이율이 0.80%, 0.86%로 확인됐다. NCBI database 자료와 비교 분석한 결과 일본의 LdMNPV와 가장 유사했으며, 터키의 LdMNPV와 가장 큰 차이를 나타냈다. 본 조사를 통하여 LdMNPV는 높은 감염율을 나타냈고 매미나방 중요한 개체군 조절인자중 한가지로 작용할 것으로 판단된다. Asian gypsy moth, Lymantria dispar asiatica (Lepidoptera: Erebidae), which is an indigenous pest in Korea, a large outbreak has been reported in Gyeonggi-do, Chungcheong-do, and northern Gyeongsangbuk-do from 2019 in forest and nearby downtown areas, causing emotional damage to forests and city dwellers. During the indoor culture of gypsy moth eggs collected in Yecheon, Gyeongbuk in 2021 we found that 79.65% (321/403 of first instar larvae) were died due to Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) infection. Sequence analyses of 36 gypsy moths collected from 12 regions in Korea using LdMNPV late expression factor-8 (lef-8) and polyhedrin (polh) genes showed a genetic variation of 0.80% and 0.86%, respectively. Comparison to GenBank data showed that the Korean samples were most similar to LdMNPV in Japan, whereas most different to those of Turkey. These results showed a high infection rate of LdMNPV in Korea and LdMNPV is one of the important population regulators of the gypsy moth.

Spatial Evaluation of Domestic Potential Distribution of Gypsy Moths (Lymantria Dispar) in Response to Climate Change

Jae-Woo SONG,Jae-Min JUNG,Youngwoo NAM,Sunghoon JUNG,Wang-Hee LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10

Recent outbreak of gypsy moths (Lymantria dispar) has caused damages on forests and landscapes in South Korea under the effects of climate change. For this reason, there is a demand on evaluating the potential distribution of gypsy moth to establish data for prevention of future damage. In this study, we evaluated the potential distribution of the gypsy moth according to climate change by using CLIMEX (version 4.0, Hearne software, Australia). Parameter values were determined based on previous studies, distribution records, and species biology; then, the model was simulated to define climatically suitable areas for establishing gypsy moth population under domestic meteorological data. The suitable areas were then projected on the map of South Korea by using ArcMap (version10. 4. 1; ESRI, Redlands, USA) so that areas at risk of gypsy moth distribution were visually identified. As a result, high climatic suitability was observed throughout the country under the current climate, whereas suitable areas were predicted to gradually decrease with climate change due to excessive summer temperature.

Parasitoid Complex of the Gypsy Moth (Lymantria dispar) in the Increase-phase Populations in Korea

이장훈,Robert W. PEMBERTON 한국생태학회 2009 Journal of Ecology and Environment Vol.32 No.2

The species composition of the parasitoid complex and the degree of parasitism by each species were analyzed for gypsy moth (Lymantria dispar) populations in the increasing phase. Total of 7,826 mid-late instar larvae and pupae were collected and reared from two collection sites in Gangwon vince, Korea. Two tachinid flies (Blepharipa schineri and Parasetigena silvestris), and the ichneumonid wasp (Coccygomimus disparis) were the most abundant parasitoids, in the order of decreasing importance. Other parasitoids occurring included Coteisa melanoscelus, Cotesia scheaferi, Glyptapanteles liparidis, a lasus, and Exorista spp. The low incidence of the NPVirus is hypothesized to be an important factor in determining degree of parasitism rate by P. silvestris and B. schineri, and their relative dominance in the parasitoid complex. The species composition of the parasitoid complex and the degree of parasitism by each species were analyzed for gypsy moth (Lymantria dispar) populations in the increasing phase. Total of 7,826 mid-late instar larvae and pupae were collected and reared from two collection sites in Gangwon vince, Korea. Two tachinid flies (Blepharipa schineri and Parasetigena silvestris), and the ichneumonid wasp (Coccygomimus disparis) were the most abundant parasitoids, in the order of decreasing importance. Other parasitoids occurring included Coteisa melanoscelus, Cotesia scheaferi, Glyptapanteles liparidis, a lasus, and Exorista spp. The low incidence of the NPVirus is hypothesized to be an important factor in determining degree of parasitism rate by P. silvestris and B. schineri, and their relative dominance in the parasitoid complex.

Discover of a male of Okinawa gypsy moth, Lymantria albescens trapped in sex-pheromone trap for AGM, Lymantria dispar

Heung-Sik Lee,Tae Hwa Kang,Gwangsu Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

A male specimen of Lymantria albescens (called as Okinawa gypsy moth) was captured in Busan, by sex-pheromone trap for Asian Gypsy Moth (AGM) (7R,8S)-cis-7,8-epoxy-2- methyloctadecane [(+)-disparlure]. Up to now, this species is distributed only in Ryukyu Islands of Japan including Ishigaki and Okinawa. The male of Okinawa gypsy moth might be attracted to AGM pheromone trap. If L. albescens is occurred in Korea, more many male individuals must be captured in pheromone trap. Therefore, we considered that the individual might be imported from Japan by inanimate pathway. Although it is high probability that L. albescens might be imported from Okinawa, it is important to a survey on an invasive pathway of the species in a view point of quarantine inspection. Through this presentation, we provided a detection method on Lymantria species using DNA barcoding. On the basis of this study, we will conduct on an invasive pathway and inhabitation possibility.

Laboratory evaluation of flight ability of the Asian Gypsy moth, Lymantria dispar (Lepidoptera: Lymantriidae)

Kwang-Su Lee,Chuleui Jung,Dongpyo Lyu 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Lymantria dispar (Linne), gypsy moth, is known as euryphagous insect and one of forest pests having wide range of host. Female of European Gypsy Moth (EGM) are flightless whereas those of the Asian Gypsy Moth (AGM) are strong fliers. So, we studied flight ability of female AGM by using flight mill device. The flight measurements of female AGM are recorded for an hour using 1- and 2-day-old mated and unmated individuals. As a results, mated females were observed more active the unmated. But, flight speed of unmated females are faster than mated. Flight frequency wasn't showed any significant difference between mated and unmated females.

Parasitism and survival rate of overwintering egg mass of Gypsy moth

Cha Young Lee,Youngwoo Nam,Jong-Kook Jung,Dong Soo Kim 한국응용곤충학회 2021 한국응용곤충학회 학술대회논문집 Vol.2021 No.04

Parasitoid Complex of the Gypsy Moth (Lymantria dispar) in the Increase-phase Populations in Korea

Lee, Jang-Hoon,Pemberton, Robert W. The Ecological Society of Korea 2009 Journal of Ecology and Environment Vol.32 No.2

The species composition of the parasitoid complex and the degree of parasitism by each species were analyzed for gypsy moth (Lymantria dispar) populations in the increasing phase. Total of 7,826 mid-late instar larvae and pupae were collected and reared from two collection sites in Gangwon Province, Korea. Two tachinid flies (Blepharipa schineri and Parasetigena silvestris), and the ichneumonid wasp (Coccygomimus disparis) were the most abundant parasitoids, in the order of decreasing importance. Other parasitoids occurring included Coteisa melanoscelus, Cotesia scheaferi, Glyptapanteles liparidis, Brachymeria lasus, and Exorista spp. The low incidence of the NPVirus is hypothesized to be an important factor in determining degree of parasitism rate by P. silvestris and B. schineri, and their relative dominance in the parasitoid complex.

매미나방 친환경 방제 소재선발에 관한 연구

안인 ( In Ahn ),이인애 ( In-ae Lee ),정호용 ( Ho-yong Jeong ),주진호 ( Jin-ho Joo ),이영돈 ( Young-don Lee ),최선희 ( Seon-hee Choi ),고득환 ( Deuk-hwan Ko ),엄재상 ( Jae-sang Eom ) 한국환경농학회 2023 한국환경농학회 학술대회집 Vol.2023 No.0

매미나방(Gypsy moth; Lymantria dispar)은 나비목 독나방과의 돌발 외래해충이다. 겨울철 이상 고온현상으로 매미나방 알집 월동 치사율이 낮아지면서 대발생으로 이어지다가 최근에는 발생이 줄어들고 있다. 2020년 19종의 과수 및 수목에 매미나방이 발생하여 피해면적은 2019년의 3배 수준인 10개 시도(89개 시군), 6,183ha에 매미나방이 대 발생하여 사과나무 등 과수 및 수목에 심한 피해를 줄 뿐만 아니라 애벌레가 사람에게 피부 가려움증을 유발하는 한편 등산객 등에게 혐오감을 유발하고 있다. 매미나방은 연 1회 발생하며 알로 월동한다. 유충은 4월경에 부화하며, 어릴 때에는 집단적으로 잎을 식해하나, 자라면서 분산한다. 5월 상순~6월 상순에 가지나 잎 사이에 실을 엮고 그 안에서 번데기가 된다. 6~7월에 성충이 되며, 수컷은 낮에 활동하나 암컷은 나무줄기에 숨어있다. 알은 나무줄기에 난괴로 약 300개를 산란한다. 밤나무, 사과나무, 배나무, 감나무, 귤나무 등을 중심으로 6월 중순부터 7월 상순까지 약충기에 방제하거나 이른 봄에 나무줄기에 있는 난괴와 집단적으로 있는 부화유충을 발견하는 대로 불태우는 것이 좋다. 방제농약으로는 프레바톤 등 10여개 농약품목과 곤충병원성 미생물인 Bt균(Bacillus thuringiensis)이 매미나방용으로 등록되어 있으나, 친환경방제제는 공시된 약제가 없어 친환경 방제제 또는 유인제 개발이 시급한 실정이다. 이에 본 연구는 정향(20% Eugenol), 데리스(5% Rotenone), 님오일(5000ppm Azadiractin) 등 식물추출물을 주제로 하고, 돼지감자, 백두옹추출물을 증량제로 사용하여 매미나방의 친환경 방제제를 개발하고자 매미나방 알집을 경북 경산에서 4월 초 매미나방 난괴를 채집 28±3℃에서 부화시킨 후 인공사료를 조제 사육하여 2~3령기에 침지법으로 실내 약효시험을 수행하는 한편 포장 약효시험은 고추밭에 망사를 씌워 접종시험을 수행하였다. 시제품 실내 약효시험결과 100, 250, 500, 750배 농도에서는 36시간 후 모두 100% 살충률을 나타내었으나 1000배 농도에서는 72시간 후 100% 사멸되는 우수한 약효를 나타냄에 따라 1000배로 시제품화하는 것이 가장 합당하다고 사료되었다. 이에 1000배 농도로 시제품화하여 포장 약효시험을 수행한 결과, 시제품(KEFAMA 8)은 고추에 접종한 매미나방에 대해 약제처리 7일 후 73.2%의 우수한 방제효과를 보였고 외관상 약해가 없어 원예작물 및 수목 등 매미나방 친환경방제제로 충분한 실용성이 있다고 판단되었다. 시제품(KEFAMA 8)이 매미나방 친환경방제제로 효과가 인정됨에 따라 향후 매미나방 유인제 개발과 함께 친환경방제제 제품화를 위한 급성 독성 확인시험과 합제형화 공정을 거쳐 상용화할 경우 매미나방 생물적 방제제로서 매미나방 확산방지에 본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 기술사업화지원 민간중심 R&D 사업의 지원을 받아 연구되었음(No. 821064-03).

Outbreak of forest insect pests in Korea: recent cases and future challenge for entomologist

Jong-Kook Jung 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.04