Electrochemical H₂O₂ biosensor composed of myoglobin on MoS₂ nanoparticle-graphene oxide hybrid structure

Yoon, Jinho,Lee, Taek,Bapurao G., Bharate,Jo, Jinhee,Oh, Byung-Keun,Choi, Jeong-Woo Elsevier 2017 Biosensors & bioelectronics Vol.93 No.-

<P><B>Abstract</B></P> <P>In this research, the electrochemical biosensor composed of myoglobin (Mb) on molybdenum disulfide nanoparticles (MoS<SUB>2</SUB> NP) encapsulated with graphene oxide (GO) was fabricated for the detection of hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>). Hybrid structure composed of MoS<SUB>2</SUB> NP and GO (GO@MoS<SUB>2</SUB>) was fabricated for the first time to enhance the electrochemical signal of the biosensor. As a sensing material, Mb was introduced to fabricate the biosensor for H<SUB>2</SUB>O<SUB>2</SUB> detection. Formation and immobilization of GO@MoS<SUB>2</SUB> was confirmed by transmission electron microscopy, ultraviolet-visible spectroscopy, scanning electron microscopy, and scanning tunneling microscopy. Immobilization of Mb, and electrochemical property of biosensor were investigated by cyclic voltammetry and amperometric i-t measurements. Fabricated biosensor showed the electrochemical signal enhanced redox current as −1.86μA at an oxidation potential and 1.95μA at a reduction potential that were enhanced relative to those of electrode prepared without GO@MoS<SUB>2</SUB>. Also, this biosensor showed the reproducibility of electrochemical signal, and retained the property until 9 days from fabrication. Upon addition of H<SUB>2</SUB>O<SUB>2</SUB>, the biosensor showed enhanced amperometric response current with selectivity relative to that of the biosensor prepared without GO@MoS<SUB>2</SUB>. This novel hybrid material-based biosensor can suggest a milestone in the development of a highly sensitive detecting platform for biosensor fabrication with highly sensitive detection of target molecules other than H<SUB>2</SUB>O<SUB>2</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The electrochemical biosensor composed of the myoglobin on the graphene oxide (GO)-encapsulated molybdenum disulfide nanoparticles (MoS<SUB>2</SUB> NP) was developed for hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>) detection. </LI> <LI> MoS<SUB>2</SUB> NP were encapsulated by GO (GO@MoS<SUB>2</SUB>) for the first time to induce the electrochemical signal enhancement. </LI> <LI> Fabricated electrochemical biosensor with GO@MoS<SUB>2</SUB> showed the stability and reproducibility. </LI> <LI> Fabricated electrochemical biosensor also showed the enhanced amperometric response current by detection of H<SUB>2</SUB>O<SUB>2</SUB>. </LI> </UL> </P>

A new coccolith modified electrode-based biosensor using a cognate pair of aptamers with sandwich-type binding

Kim, Sang Hoon,Nam, Onyou,Jin, EonSeon,Gu, Man Bock Elsevier 2019 Biosensors & bioelectronics Vol.123 No.-

<P><B>Abstract</B></P> <P>In this study, we report a cognate pair of the aptamer-based sandwich-type electrochemical biosensor for type 2 diabetes biomarker (Vaspin) using coccolith modified electrodeposited on the screen-printed gold electrode (CME-SPGE). The coccolith derived from E. huxleyi used in this study were known to be highly-structured microparticles with many nano-sized pores. The CME-SPGE was successfully fabricated by drop-casting coccoliths, followed by Au sputtering and electrodeposition of Au. On this CME-SPGE electrode, the sandwich-type electrochemical aptasensor was fabricated by using a cognate pair of aptamers. The morphological, electrochemical characteristics and the performances of both the CME-SPGE and the completely fabricated sandwich-type aptasensor were investigated by SEM, EDAX, cyclic voltammetry, and chronoamperometry. Due to the synergic effect of a cognate pair of aptamers on CME-SPGE, this newly developed sandwich-type electrochemical biosensor for Vaspin showed high specificity, and good sensitivity with a limit of detection (LOD) of 298 pM, along with more widen the linear range. To the best of our knowledge, this is the first report about the use of a coccolith modified electrode with a cognate pair aptamer resulting in sandwich-type binding in an electrochemical biosensor. With the advantages of using highly-structured biomineral microparticles and a cognate pair of aptamers, this new study may pave the innovative way to design a novel sandwich-type electrochemical aptasensor platform.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The original SPGE surface was successfully modified uniformly with the highly-structured coccolith microparticles. </LI> <LI> A cognate pair of aptamers was applied to fabricate sandwich-type electrochemical aptasensor successfully. </LI> <LI> This newly developed sandwich-type electrochemical biosensor was highly sensitive to type 2 diabetes biomarker, Vaspin. </LI> </UL> </P>

Biosensors for rapid and sensitive detection of <i>Staphylococcus aureus</i> in food

Rubab, Momna,Shahbaz, Hafiz Muhammad,Olaimat, Amin N.,Oh, Deog-Hwan Elsevier 2018 Biosensors & bioelectronics Vol.105 No.-

<P><B>Abstract</B></P> <P>Foodborne illness outbreaks caused by the consumption of food contaminated with harmful bacteria has drastically increased in the past decades. Therefore, detection of harmful bacteria in the food has become an important factor for the recognition and prevention of problems associated with food safety and public health. <I>Staphylococcus aureus</I> is one of the most commonly isolated foodborne pathogen and it is considered as a major cause of foodborne illnesses worldwide. A number of different methods have been developed for the detection and identification of <I>S. aureus</I> in food samples. However, some of these methods are laborious and time-consuming and are not suitable for on-site applications. Therefore, it is highly important to develop rapid and more approachable detection methods. In the last decade, biosensors have gained popularity as an attractive alternative method and now considered as one of most rapid and on-site applicable methods. An overview of the biosensor based methods used for the detection of <I>S. aureus</I> is presented herein. This review focuses on the state-of-the-art biosensor methods towards the detection and quantification of <I>S. aureus,</I> and discusses the most commonly used biosensor methods based on the transducing mode, such as electrochemical, optical, and mass-based biosensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recent advances in development of biosensors for detection of <I>S. aureus</I> are discussed. </LI> <LI> An overview of biosensors based on transducing mode is presented. </LI> <LI> Electrochemical, optical, and mass-based biosensors are mainly discussed. </LI> </UL> </P>

Effect of nanostructured MoS2 morphology on the glucose sensing of electrochemical biosensors

Dinh Van Tuan,Dang Thi Thuy Ngan,Nguyen Thi Thuy,Hoang Lan,Nguyen Thi Nguyet,Vu Van Thu,Vuong-Pham Hung,Phuong Dinh Tam 한국물리학회 2020 Current Applied Physics Vol.20 No.9

In this study, the effects of the morphological characteristics of MoS2 nanomaterials on the glucose sensing of electrochemical biosensors were explored. Nanostructured MoS2 materials, including nanoparticles (NPs), nanoflowers (NFs), and nanoplatelets (NPLs), were prepared via a simple hydrothermal method. The structure and morphological characteristics of MoS2 nanomaterials were examined through X-ray diffraction, field emission scanning electron microscopy, and Raman spectroscopy. Electrochemical properties were analyzed through cyclic voltammetry. Results showed that the obtained sensitivity was 64, 68.7, and 77.6 μAmM 1 cm 2 for MoS2 NP-, MoS2 NF-, and MoS2 NPL-based biosensors, respectively. The limit of detection (LOD) of all MoS2-based glucose biosensors was 0.081 mM. In addition, the pH, temperature, glucose oxidase (GOx) concentration, reproducibility, specificity, and stability of glucose biosensors with different MoS2 morphologies were also investigated and indicated the oxidation current response of the MoS2 NPL-based glucose biosensor was higher than that of MoS2 NF- and NP-based biosensors.

Comparison of the sensitivity of thiolated aptamer based biosensor according to the condition of electrode substrates

정세훈,Changsung Sean Kim,양정승 한국바이오칩학회 2010 BioChip Journal Vol.4 No.2

Recently, aptamers have been considered as a great alternative to antibodies to develop biosensors because aptamers can strongly bind a specific target molecule. And the aptamer shows the possibility for the commodity industry in developing biosensor because of artificial receptors. Using these aptamers, therefore, many aptamer based electrochemical biosensors have been studied by applying new materials and surface treatment to substrates. However, comparing degree of the biosensor sensitivity in the same aptamers according to the each condition of electrode substrates was still not reported. In this study, we have studied on the sensitivities by changing the experimental conditions for area, material and surface treatment of electrode substrates to develop aptamer based biosensors using thrombin DNA aptamers. The results indicate that the larger exposed area of electrodes is more efficient than the smaller. Moreover, the sensitivity in the screen-printed carbon electrode and GNP/swCNT/mwCNT treated carbon electrode showed the remarkable efficiency than in gold or platinum electrodes. We believe this study can be employed as reference data for developing the commercial aptamer based biosensor.

Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus

Kwon Nayeon,Lee Siyun,Jang Moonbong,Lee Jin-Ho,Park Chulhwan,Lee Taek 한국바이오칩학회 2024 BioChip Journal Vol.18 No.1

Yellow fever virus (YFV) is an acute infectious virus with high morbidity and mortality risks during the toxic phase. Early diagnosis and suppression are essential because YFV has no precise treatment. With the aim of detecting YFV, we fabricated a highly sensitive electrochemical biosensor comprised with a truncated DNA aptamer/MXene heterolayer. The synthesized DNA aptamer was prepared by systematic evolution of ligands using the exponential enrichment (SELEX) technique, which can specifically detect the YFV NS1 protein. MXenes increase the electrical sensitivity and the possibility of attachment of aptamers by widening the surface area. The aptamer-cutting process which called a truncation process can reduce the production cost of biosensors. The biosensor performance was evaluated using electrochemical methods, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The limit of detection (LOD) was 2.757 pM for YFV diluted in phosphate-buffered saline (PBS) and 2.366 pM for YFV diluted in 10% human serum, proving that the biosensor specifically binds to YFV through selectivity evaluation. This biosensor can be a valuable tool for the early diagnosis of YFV, enabling timely intervention as well as facilitating the control and prevention of yellow fever outbreaks.

Recent advances in noninvasive flexible and wearable wireless biosensors

Salim, Ahmed,Lim, Sungjoon Elsevier 2019 Biosensors & bioelectronics Vol.141 No.-

<P><B>Abstract</B></P> <P>Flexible and wearable biosensors have great potential to interface with skin and noninvasively extract biofluids for real time and continuous monitoring of physiological status. Soft electronics is the prime factor in these wearables, regardless of sensing mechanism and fabrication technology. Wireless connectivity would be a valuable addition to enhance wearable biosensor's scope for remote and resource limited settings. These skin-adaptable, user-friendly, battery-free (although not all), and noninvasive devices continuously and simultaneously monitor wearer well-being and transfer data wirelessly. Thus, they have a great potential to improve quality of life with timely diagnostics and hence early treatments. However, they remain in the early stages, with relatively conventional sensing modalities, battery requirements, soft electronics fabrication limitations, and practicable size restrictions to retain skin compatibility. We classify wearable biosensors by sensing functionality, such as skin temperature, pH, heart rate, sweat glucose, uric acid, sweat electrolyte, cerebrospinal shunt flow, and toxic chemicals, and discuss challenges and prospects for these biosensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> State-of-the-art flexible and wearable wireless biosensors (WWBs) are discussed in this comprehensive review. </LI> <LI> Significance of wearable biosensors in addition with wireless connectivity are established. </LI> <LI> Commonly used stretchable materials and fabrication techniques for wearable biosensors are studied. </LI> <LI> Design strategies of WWBs are studied. </LI> <LI> Multivariate sensing platforms utilizing sweat and tear fluids are emphasized. </LI> <LI> Single analyte monitoring for metabolite levels, vital signs, body motion and cerebrospinal shunt are discussed. </LI> <LI> Finally, challenges and prospects for WWBs are discussed. </LI> </UL> </P>

A highly stretchable and conductive 3D porous graphene metal nanocomposite based electrochemical-physiological hybrid biosensor

Xuan, Xing,Kim, Ji Y.,Hui, Xue,Das, Partha S.,Yoon, Hyo S.,Park, Jae-Yeong Elsevier 2018 Biosensors & bioelectronics Vol.120 No.-

<P><B>Abstract</B></P> <P>Recently, highly stretchable and flexible electrodes essential for wearable electronic devices has been reported. However, their electrical resistances are high, the fabrication processes are complicated and involve a high cost, and deformations such as stretching can lead to the degradation on electrical performance. To address these issues, a novel fabrication process (both inexpensive and simple) for the highly stretchable and conductive electrodes using well patterned 3D porous laser-induced graphene silver nanocomposite was developed. The fabricated electrode exhibited a high, uniform electrical conductivity even under mechanical deformations. Addition of platinum and gold nanoparticles (PtAuNP) on the 3D porous LIG greatly improved the electrochemical performance for wearable glucose sensor applications. The fabricated glucose sensor exhibited low detection limit (5 µM), and acceptable detection range from 0 to 1.1 mM (covers the glucose range in sweat), and high linearity (0.99). In addition, the fabricated pH sensor also exhibited a linear response (66 mV/pH) at the range from 4 to 7. This work successfully demonstrates the potential of this novel fabrication technique and stretchable LIG metal nanocomposite for wearable electrochemical-physiological hybrid biosensors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel process for the highly stretchable and conductive electrodes was developed using well patterned 3D porous LIG silver nanocomposite. </LI> <LI> Platinum and gold nanoparticles (PtAuNP) were deposited on the 3D porous LIG and it greatly improves the electrochemical performance. </LI> <LI> Human sweat samples were used and acceptable results were obtained using electrochemical-physiological hybrid biosensors. </LI> </UL> </P>

Electrochemical Immunosensor Using a Gas Diffusion Layer as an Immobilization Matrix

Kim, Yong-Tae,Oh, Kyu-Ha,Kim, Joo-Ho,Kang, Hee-Gyoo,Choi, Jin-Sub Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.6

The modification of a gas diffusion layer (GDL), a vital component in polymer electrolyte fuel cells, is described here for use in the electrochemical detection of antibody-antigen biosensors. Compared to other substrates (gold foil and graphite), mouse anti-rHBsAg monoclonal antibody immobilized on gold-coated GDL (G-GDL) detected analytes of goat anti-mouse IgG antibody-ALP using a relatively low potential (-0.0021 V vs. Ag/AgCl 3 M NaCl), indicating that undesired by-reactions during electrochemical sensing should be avoided with G-GDL. The dependency of the signal against the concentration of analytes was observed, demonstrating the possibility of quantitative electrochemical biosensors based on G-GDL substrates. When a sandwich method was employed, target antigens of rHBsAg with a concentration as low as 500 ng/mL were clearly measured. The detection limit of rHBsAg was significantly improved to 10 ng/mL when higher concentrations of the 4-aminophenylphosphate monosodium salt (APP) acting on substrates were used for generating a redox-active product. Additionally, it was shown that a BSA blocking layer was essential in improving the detection limit in the G-GDL biosensor.

Recent Advances in Biosensors for Nucleic Acid and Exosome Detection

Zirui Fu,Yicheng Lu,James J. Lai 전남대학교 의과학연구소 2019 전남의대학술지 Vol.55 No.2

Biosensors are analytical devices for biomolecule detection that compromise three essential components: recognition moiety, transducer, and signal processor. The sensor converts biomolecule recognition to detectable signals, which has been applied in diverse fields such as clinical monitoring, in vitro diagnostics, food industry etc. Based on signal transduction mechanisms, biosensors can be categorized into three major types: optical biosensors, electrochemical biosensors, and mass-based biosensors. Recently, the need for faster, more sensitive detection of biomolecules has compeled researchers to develop various sensing techniques. In this review, the basic structure and sensing principles of biosensors are introduced. Additionally, the review discusses multiple recent works about nucleic acid and exosome sensing.