나방의 성페로몬 감지

Kye Chung Park 한국응용곤충학회 2022 한국응용곤충학회지 Vol.61 No.1

Moths have a well-developed sex pheromone communication system. Male moths exhibit an extremely sensitive and selective sex pheromone detection system so that they can detect the sex pheromone produced by conspecific females and locate them for successful mating. Using the pheromone detection system, male moths display characteristic stereotypic behavioral responses, flying upwind to follow intermittent filamentous pheromone strands in pheromone plume. The chemical composition of female sex pheromone in moths, typically comprised of multiple compounds, is species-specific. Male moths contain specialized pheromone receptor neurons on the antennae to detect conspecific sex pheromone accurately, and distinguish it from the pheromones produced by other species. The signals from pheromone receptor neurons are integrated and induce relevant behavior from the male moths. Male moths also contain olfactory sensory neurons in pheromone sensilla, specialized for pheromone-related behavioral antagonist compounds, which can enhance discrimination between conspecific and heterospecific pheromones. Here we review reports on the sex pheromone detection system in male moths and their related responses, and suggest future research direction. 나방은 성페로몬에 대한 통신시스템이 잘 발달되어 있다. 동종의 암컷이 방출하는 성페로몬을 원거리에서 감지하여 암컷을 정확히 찾아가 교미할 수 있도록 하기 위해서, 수컷 나방은 고도로 발달된 성페로몬 감지 시스템을 갖고 있다. 이러한 시스템을 이용해서 수컷 나방은 페로몬 냄새 기둥(plume)을 따라 바람을 거슬러 비행하면서 간헐적으로 감지되는 페로몬 냄새가닥(odor filaments)을 추적하는 고정행동양식(stereotypic behavior)을 보인다. 일반적으로 여러 성분으로 구성되는 나방의 암컷 성페로몬은 그 조성이 종특이적(species-specific)이며, 비슷한 성분을 공유하는 유사종들이 방출하는 성페로몬과 동종의 암컷이 방출하는 성페로몬을 정확히 구분하기 위해서 수컷 나방은 촉각에 여러 종류의 고도로 특화된 페로몬 감각세포들을 갖고 있어서, 이들이 페로몬을 감지할 때 나오는 신경 신호들을 종합해서 동종의 페로몬을 인식하여 행동반응이 일어 나게 된다. 수컷 나방은 보통 동종의 페로몬 성분뿐만 아니라 유사종이 사용하는 페로몬 성분들을 특이적으로 감지하는 길항적(antagonistic) 냄새감각세포들도 갖고 있어서 페로몬 식별력을 강화한다. 본 종설에서는 지금까지 보고된 수컷 나방의 페로몬 감지 시스템과 이와 연관된 수컷의 감각기 및 행동반응에 대한 연구 결과들을 정리하고, 이를 종합하여 앞으로의 연구 방향을 제시하고자 한다.

Some Responses of Cryptic Mealybug, Pseudococcus cryptus, for a Synthetic Sex Pheromone in Citrus Orchards, Jeju

Jeong Heub Song,Kyung San Choi,Soon Yeong Hong,Sin Chan Lee 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Some responses of cryptic mealybug, Pseudococcus cryptus, for a sex pheromone, [(1R,3R)-3-isopropenyl-2,2-dimethylcyclobutyl]methyl 3-methyl-3-butenoate, were studied to develop the applying strategy of sex pheromone trap for efficient monitoring and control-time decision in citrus orchards grown in greenhouse for 2 years (2007-2008). The optimal concentration of a sex pheromone was 1.0㎎ per hexane 100㎕ in a using pheromone lure. There was no significant effect of the attraction of male adults among different colours (white, blue and yellow) of plate-type trap baited with sex pheromone. The more male adults were attracted, as the distance of a pheromone trap form a citrus tree infested with cryptic mealybug was closer. The male adults were caught to sex pheromone trap from middle April to late November, and its peak period was the middle of July. The thermal constant for the next attraction was about 640 degree-days (lower threshold temperature=12℃) after confirmed the first attraction of male adults. The optimum control time for cryptic mealybug after the first attraction of male adults were confirmed was estimated as 450 to 500 degree-days.

Olfactory Responses of Male and Female Red-spelled Newts to Sex Pheromones from the Opposite Sex

Park, DaeSik,Park, Shi-Ryong The Korean Society for Integrative Biology 2002 Korean journal of biological sciences Vol.6 No.4

Functional characterization of sex pheromones in olfactory responses is essential for the study of chemical communications in amphibians. Using Y-maze olfactory preference tests, we have investigated the olfactory res-ponses of male and female red-spotted newts, Notophthalmus viridescens, to the crude extracts of the opposite sex's genial and cloacal pheromones. Both male and female cloacal pheromone extracts caused the opposite sex to initiate olfactory responses by leaving the starting area in the Y-maze, but only subject males exposed to female cloacal pheromone extracts completed olfactory choice by entering the side arm of the Y-maze which received the pheromones. For genial pheromone extracts, only female genial pheromone extracts induced initial olfactory responses from test males. Neither male nor female genial pheromone extracts made the oppo-site sex complete olfactory choice. Pre-exposure of test females to male pheromone extracts increased the likelihood of initial olfactory responses. The latency for initial olfactory responses of test females that were previously exposed to male genial extracts was significantly shorter than that of control females.

Field test of the synthetic sex pheromone in the rice green caterpillar, Naranga aenescens and the rice leaf roller, Cnaphalocrocis medinalis

Ki Hong Yum,Jum Rae Cho,Minho Lee,Young Nam Youn 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

This study was carried out to investigate the effect of each single component of the synthetic sex pheromone in the rice green caterpillar, Naranga aenescens and rice leaf roller, Cnaphalocrocis medinalis. Trap catches with each single component or binary mixtures of the sex pheromone in N. aenescens were significantly lower than that with an optimum mixture (2: 1: 4) of Z9-14:Ac, Z9-16:Ac and Z11-16:Ac. But only few number of C. medinalis male was caught in trap baited with each component of the sex pheromone excepting Z13-18:Ald. In an optimum composition of the sex pheromone in C. medinalis, trap catches baited with an 11: 100: 11 mixture of Z11-18:Ald, Z13-18:Ald, and Z13-18:Ac without two alcohol components (Z11-18:OH and Z13-18:OH) was higher than that baited with an optimum mixture of Z11-18:Ald, Z13-18:Ald, Z11-18:OH, Z13-18:OH, and Z13-18:Ac. The efficacy of the sex pheromone lure of N. aenescens kept over 4 weeks. The occurrence of N. aenescens caught in trap baited with sex pheromone gave three peaks and C. medinalis showed two distinct peaks throughout the flight season. Therefore, it can be expected that pheromone-baited trap provides a simple tool for monitoring N. aenescens and C. medinalis in Korea.

Identification of the male produced aggregation-sex pheromone of Monochamus saltuarius, a major insect vector of the pine wood nematode in Korea

Hyo-Rim Lee,Sung-Chan Lee,Dong Ha Lee,Won-Sil Choi,Chan-Sik Jung,Jae-Ho Jeon,Jeong-Eun Kim,Il-Kwon Park 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.10

In this study, we isolated and identified an aggregation-sex pheromone from Monochamus saltuarius, the major insectvector of the pine wood nematode in Korea. Adult male of M. saltuarius produces 2-undecyloxy-1-ethanol, which is knownto be an aggregation-sex pheromone in other Monochamus species. We performed field experiments to determine the attractivenessof the pheromone and other synergists. More M. saltuarius adult beetles were attracted to traps baited with the pheromonethan to unbaited traps. Ethanol and (-)-α-pinene interacted synergistically with the pheromone. Traps baited with pheromone+(-)-α-pinene+ethanol were more attractive to M. saltuarius adults than traps baited with pheromone, (-)-α-pinene, or ethanol alone.Ipsenol, ipsdienol, and limonene were also identified as synergists of the aggregation-sex pheromone for M. saltuarius adults.

Two terpenoids activates close mating behavior and enhances trap efficiency of sex pheromone of Grapholita molesta

Hui-Ming Xiang,Zhen Chen,Xian-Wei Li,Yan-Qiong Guo,Xian-Chun li,Rui-Yan Ma 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.4

Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is a notorious pest of many Rosaceae crops worldwide. Enhancement of trap efficiency of its sex pheromone was devised by addition of E-β-ocimene and E-β-farnesene. The addition of E-β-ocimene or E-β-farnesene to sex pheromone increased electroantennogram response of male G. molesta compared to sex pheromone alone. Blend of pheromone and E-β-ocimene or E-β-farnesene in 1:0.1 increased the upwind flight and landing behaviors. Furthermore, field experiments showed that sex lures with Eβ-ocimene, or /and E-β-farnesene, enhanced trapping efficiency compared to sex pheromone alone. These results may provide the basis for the development of efficient pest management systems against G. molesta using plant volatiles and insect sex pheromones.

Development of Sex Pheromone Trap for Monitoring Matsumuraeses falcana (Walshingham) (Lepidoptera: Tortricidae)

Cho, Jum-Rae,Choi, Kyung-San,Jung, Jin-Kyo,Park, Jong-Ho,Seo, Bo-Yoon Korean Society of Applied Entomology 2007 Journal of Asia-Pacific Entomology Vol.10 No.4

This study was carried out to find out an effective formulation for monitoring the soybean podborer, Matsumuraeses falcana (Walshingham), using its sex pheromone in Korea. A 1 : 1 : 1 mixture of (E)-8-dodecenyl acetate (E8-12:Ac), (E,E)-8,10-dodecadienyl acetate (E8E10-12:Ac) and (E,Z)-7,9-dodecadienyl acetate (E7Z9-12:Ac) was an effective composition in monitoring M. falcana. A binary mixture of E8-12:Ac and E8E10-12:Ac was essential to attract M. falcana male moths. E7Z9-12:Ac showed a synergistic effect on the attractiveness of 1 : 1 mixture of E8-12:Ac and E8E10-12:Ac. These chemicals alone showed little or no attractiveness to M. falcana. The sticky-typed wing trap baited with sex pheromone captured significantly more males than the water-pan trap. Trap catches within a soybean field were much more than those in perimeter trap. The trap-baited with sex pheromone gave a distinct fluctuation pattern of male catches throughout the flight seasons. Therefore, it can be expected that pheromone-baited trap provides a simple tool for monitoring the soybean podborer, M. falcana in Korea.

Mating disruption of the carpenter moth, Cossus insularis (Staudinger) (Lepidoptera: Cossidae) with synthetic sex pheromone in Japanese pear orchards

Tomoaki Nakanishi,Kiyoshi Nakamuta,Fumiaki Mochizuki,Takehiko Fukumoto 한국응용곤충학회 2013 Journal of Asia-Pacific Entomology Vol.16 No.3

Mating disruption of the carpenter moth, Cossus insularis Staudinger (Lepidoptera: Cossidae), with a synthetic version of its sex pheromone, amixture of (E)-3-tetradecenyl acetate and (Z)-3-tetradecenyl acetate, was tested for three successive years in Japanese pear (Pyrus pyrifolia var. culta) orchards. Pheromone trap catches,percentage mating of tethered females, and tree damage were measured in both the pheromone-treated and untreated control orchards. The attraction of male adults to pheromone traps was completely disrupted, and the mating of the tethered females was completely inhibited by the treatment of synthetic pheromones. The percentage of damaged trees in the pheromone-treated orchard decreased over the course of the experiment,while the damage percentage did not decrease in the untreated orchard. These results show that mating disruption with the synthetic sex pheromone is promising for the reduction of damage caused by C. insularis in apple and Japanese pear orchards.

Sex Pheromone Composition of the Diamondback Moth, Plutella xylostella (L.) in Korea

Lee, Suk,Lee, Dae-Weon,Boo, Kyung-Saeng Korean Society of Applied Entomology 2005 Journal of Asia-Pacific Entomology Vol.8 No.3

The mating behavior of the diamondback moth (DBM), Plutella xylastella, was actively observed from 2 h post-scotophase and maintained up to 2 h post-photophase. The pheromone glands of DBM populations in Korea were extracted with hexane and gas chromatograph (GC) analysis showed that three major components of sex pheromone, (Z)-11-hexadecenal (Z11-16:Al), (Z)-11-hexadecenyl acetate (Z11-16:Ac) and (Z)-11-hexadecenyl alcohol (Z11-16:OH), were identified at the ratio of 8:3:2. To contrast, Z11-16:OH was not detected from Solid Phase Microextraction for the sex pheromone emitted during the scotophase. Both Z11-16:Al and Z11-16:Ac elicited the antennal response of male DBM in GC-electroantennographic detection and electroantennogram (EAG) assay. EAG assay revealed that the ratio of major components, Z11-16:Al and Z11-16:Ac, in the blend of 5:5 and 7:3 strongly attracted DBM male adults. The minor components, 0.5% of Z11-16:OH or 5 to 10% of (Z)-9-tetradecenyl acetate (Z9-14:Ac) also elicited the EAG response when mixed with major components blends at the ratio of 10:10 between Z11-16:Ac and Z11-16:Al, suggesting these components affect the activity of major components of sex pheromone.

성페로몬 감각생리와 해충관리기술

김용균 한국응용곤충학회 2021 한국응용곤충학회지 Vol.60 No.1

성페로몬은 곤충 종 특이적으로 교미신호를 전달하는 화학신호물질이다. 곤충의 촉각에는 이러한 성페로몬 화학물질을 받아들이는 특이적 수용체를 지닌다. 성페로몬이 이 수용체에 결합하면서 감각전위를 발생시키고 이는 대뇌로 전달되어 정보 인식을 통해 교미행동을 유발하게 한다. 성페로몬은 또한 해충의 발생을 모니터링하는 데 이용되어 온도발육모델과 더불어 향후 발생상황을 예측하는 데 널리 이용되고 있다. 더불어 성페로몬이 해충의 대량포획, 유살 또는 교미교란을 유발하여 직접적으로 방제에 응용된다. 본 종설은 성페로몬과 관련된 곤충 생리 및 이를 이용한 해충관리기술을 소개한다. Sex pheromone is used for chemical communication for mating in a species-specific manner in insects. Insect antennae possess sensory receptors specific to sex pheromone components and generate receptor potential to be perceived by the brain to evoke mating behavior. The sex pheromones have been used for monitoring specific species of insect pests to predict their subsequent occurrences based on a temperature-dependent growth model. Sex pheromones are also used for controlling pest insects using several different strategies such as mass capture, lure-and-kill, or mating disruption. This review explains the sensory physiology and insect pest management techniques related with sex pheromone.