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Yan, Zhen,Ma, Yu Zhen,Liu, Dong jun,Cang, Ming,Wang, Rui,Bao, Shorgan Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4
RNA interference (RNAi) is an acknowledged useful and effective tool to study gene function in various cells. Here, we suppressed the Connexin 43 (Cx 43) gene expression during in vitro development of ovine pre-implantation embryos using the RNAi method. The 353 bp Cx 43 double-stranded RNA was microinjected into in vitro fertilized ovine zygotes, and the levels of target mRNA and protein were investigated. Control groups included uninjected zygotes or those injected with RNase-free water. The dsRNA injection resulted in the specific reduction of Cx 43 transcripts as analyzed by quantitative real-time RT-PCR and decreased protein levels as shown by Western blot analysis at the blastocyst stage. Microinjection of Cx 43 dsRNA led to 20.3%, 21.7% and 34.5% blastocyst rates and 19.2%, 37.5% and 41.3% hatched blastocyst rates in Cx 43 dsRNA-injected, water-injected and uninjected groups, respectively. Then the RNAi could not significantly affect cell number and cell death rates of blastocysts. Therefore, suppression of Cx 43 dsRNA and proteins did not apparently affect the development potential of ovine pre-implantation embryos but may play a role in embryo quality. RNAi technology is a promising approach to study gene function in early ovine embryogenesis.
Online Integrated Development Environment for MapReduce Programming
Zhiqiang Ma,Shuangtao Yang,Zhida Shi,Rui Yan 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.6
Though MapReduce programming model simplifies the development of parallel program, ordinary users have difficulties in setting up the development environment for MapReduce. The online integrated development environment for MapReduce programming can solve this problem, thus users need not build the environment themselves, only need to focus on the logical design of the parallel program. During the software construction, the problem of independent space setting and naming conflict of the file in the multi-user environment, and the problem of online compiling, execution and instant feedback message to client are solved. The software has been deployed and tested in Hadoop cluster, and can meet users’ basic requirements for the development of MapReduce.
HOCl Oxidation-modified CT26 Cell Vaccine Inhibits Colon Tumor Growth in a Mouse Model
Zhou, Rui,Huang, Wen-Jun,Ma, Cong,Zhou, Yan,Yao, Yu-Qin,Wang, Yu-Xi,Gou, Lan-Tu,Yi, Chen,Yang, Jin-Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Despite progress in elucidating mechanisms associated with colorectal cancer and improvement of treatment methods, it remains a frequent cause of death worldwide. New and more effective therapies are therefore urgently needed. Recent studies have shown that immunogenicity of whole ovarian tumor cells and subsequent T cell response were potentiated by oxidation modification with hypochlorous acid (HOCl) in vitro and ex vivo. These results prompted us to investigate the protective antitumor response with an HOCl treated CT26 colorectal cancer cell vaccine in an in vivo mouse model. Administration of HOCl modified vaccine triggered robust antitumor immunity to autologous tumor cells in mice and prolonged survival period significantly. In addition, increased necrosis and apoptosis were found in tumor tissue from the oxidation group. Interestingly, ELISPOT assays showed that specific T cell responses were not elicited in response to the immunizing cellular antigen, in contrast to raising sera antibody titer and antibody binding activity shown by ELISA assay and flow cytometry. Further evaluation of the mechanisms underlying HOCl modified vaccine mediated humoral immunity highlighted the role of antibody-dependent cell-mediated cytotoxicity. These results combined with previous studies suggest that HOCl oxidation modified whole cell vaccine has wide applicability as a cancer vaccine because it can target both T cell- and B cell-specific responses. It may thus represent a promising approach for the immunotherapy of colorectal cancer.
( Yunlei Han ),( Rui Wang ),( Zhirong Yang ),( Yuhua Zhan ),( Yao Ma ),( Shuzhen Ping ),( Liwen Zhang ),( Min Lin ),( Yongliang Yan ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.7
1-Aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate the growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encodingACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared with the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing the salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plants.
ELECTRIC-FIELD DEPENDENCE OF MOLECULAR CONFORMATIONS OBSERVED BY USING SCANNING TUNNELING MICROSCOPY
XIAO JING MA,RUI ZHANG,YONG TAO SHEN,XIAO HUI QIU,YAN LIAN YANG,CHEN WANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.2
We review the progress in observation of electrically induced conformational changes of a range of single molecules and molecular assemblies using scanning tunneling microscopy (STM). Recent results using species with optical active functional groups and supramolecular structures confirmed the previously observed effects that the cholesterol molecules with soft linkers have the conformational bistability when switching the bias polarity, while no discernable changes were observed for the mesogen molecules, containing rigid linking units. In addition, it was also observed that the linker units could have appreciable impacts on the assembling characteristics.
Zhang, Jun,Wang, Rui,Ma, Yan-Yun,Chen, Lin-Qi,Jin, Bo-Han,Yu, Hua,Wang, Jiu-Cun,Gao, Chun-Fang,Liu, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers in the world and deeply threatens people's health, especially in China. Techniques of early diagnosis, prevention and prediction are still being discovered, among which the approaches based on single nucleotide polymorphisms in microRNA genes (miRNA SNPs) are newly proposed and show prospective potential. In particular, the association between SNPs in miRNA196a-2 (rs11614913) and miRNA146a (rs2910164) and HCC has been investigated. However, the conclusions made were conflicting, possibly due to insufficient sample size or population stratification. Further confirmations in well-designed large samples are still required. In this study, we verified the association between these two SNPs and the susceptibility to HCC by MassARRAY assay in a 2,000 large Chinese case-control sample. Significant association between rs11614913 and HCC was confirmed. Subjects with the genotype of CT+TT or T allele in rs11614913 were more resistant to HCC (CT+TT: OR (95% CI)=0.73 (0.57-0.92), P=0.01; T allele: OR (95% CI)=0.85 (0.75-0.97), P=0.02) and HBV-related HCC (CT+TT: OR (95% CI)=0.69 (0.53-0.90), P=0.01; T allele: OR (95% CI)=0.82 (0.71-0.95), P=0.01). The affected carriers of CT or TT also tended to have lower levels of serum AFP (P=0.01). This study demonstrated a role of rs11614913 in the etiology of HCC. Further research should focus on the clinical use of this miRNA SNP, so as to facilitate conquering HCC.
Hui-Ming Xiang,Rui-Yan Ma,Hong-Liang Diao,Xian-Wei Li,Xiao-Juan He,Yong-Fu Guo 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
The oriental fruit moth (OFM), Grapholita molesta (Lepidoptera: Tortricidae), is a serious pest of stone and pome fruit trees worldwide. G. molesta can switch hosts and produce multiple generations per year. Both the primary and secondary host plants of G. molesta emit aldehydes. Here, we tested whether these aldehydes are helpful for host switching of G. molesta. SPME (Solid Phase Microextraction)-GC–MS analysis identified four aldehydes (octanal, nonanal, decanal, and benzaldehyde) from volatiles of late-season yellow peach fruits. Of the four aldehydes, eletroantennogram analysis showed that nonanal produced the strongest response from the antennae of virgin and mated females. In Y-tube bioassays, only nonanal was attractive to mated G. molesta females amongst the four aldehydes, suggesting that nonanal is important for host recognition in G. molesta. In a field trial, each of the four aldehydes was tested as an attractant for both sexes of G. molesta. The traps baited with nonanal captured more female G. molesta, as compared to traps with other aldehydes and control; the traps baited with benzaldehyde captured most male G. molesta. Our research investigations provide theoretical foundation for biological control of G. molesta females. Future G. molesta lure designs should take into account sex-specific differences in the response to individual aldehydes.