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Lim, Jong Chul,Lee, Jeong Ral,Lim, Jal Yun,Min, Kyung Rak,Kim, Chi Kyung,Kim, Young Soo 한국미생물 · 생명공학회 2000 Journal of microbiology and biotechnology Vol.10 No.2
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (HOPDA) hydrolase catalyzes the hydrolytic cleavage of HOPDA to benzoate and 2-hydroxypenta-2,4-dienoate (HPD) during microbial catabolism of biphenyl and polychlorinated biphenyls. A HOPDA hydrolase gene (pcbD) was isolated from the genomic library of Pseudomonas sp. P20 and designated as pCNU1201; a 7.5-kb XbaI DNA fragment from Pseudomonas sp. P20 was inserted into the pBluescript SK(+) XbaI site. E. coli HB101 harboring pCNU1201 exhibited HOPDA hydrolase activity. The open reading frame (ORF) corresponding to the pcbD gene consisted of 855 base pairs with an ATG initiation codon and a TGA termination codon. The ORF was preceded by a ribosome-binding sequence of 5'-TGGAGC-3' and its G+C content was 55mol%. The pcbD gene of Pseudomonas sp. P20 was located immediately downstream of the pcbC gene encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase, and approximately 4-kb upstream of the pcbE gene encoding HPD hydratase. The pcbD gene was able to encode a polypeptide with a molecular weight of 31,732 containing 284 amino acid residues. The deduced amino acid sequence of the HOPDA hydrolase of Pseudomonas sp. P20 exhibited high identity (62%) with those of the HOPDA hydrolases of P putida KF715, P pseudoalcaligenes KF707, and Burkholderia cepacia LB400, and also significant homology with those of other hydrolytic enzymes including esterase, transferase, and peptidase.
Identification of Proteomic Components Associated with Resistance to Fusarium Head Blight in Rye
Dawid Perlikowski,Halina Wiś,niewska,Tomasz Gó,ral,Piotr Ochodzki,Maciej Majka,Izabela Pawłowicz,Jolanta Belter,Arkadiusz Kosmala 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4
Rye was used here to dissect molecular mechanisms of resistance to Fusarium head blight (FHB) and to go deeper with our understanding of that process in cereals. F. culmorum-damaged kernels of two lines different in their potential of resistance to FHB were analyzed using two-dimensional gel electrophoresis and mass spectrometry to identify resistance markers. The proteome profiling was accompanied by measurements of a- and b-amylase activities and mycotoxin content. The proteomic studies indicated a total of 18 spots with clear differences in protein abundance between the more resistant and more susceptible rye lines after infection. Eight proteins were involved in carbohydrate metabolism of which six proteins showed a significantly higher abundance in the resistant line. The other proteins recognized here were involved in stress response and redox homeostasis. Three remaining proteins were associated with protease inhibition/resistance and lignin biosynthesis, revealing higher accumulation levels in the susceptible rye line. After inoculation, the activities of a- and b-amylases, higher in the susceptible line, were probably responsible for a higher level of starch decomposition after infection and a higher susceptibility to FHB. The presented results could be a good reference for further research to improve crop resistance to FHB.
Relationships between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars
Tomasz Góral,Kinga Stuper-Szablewska,Maciej Buśko,Maja Boczkowska,Dorota Walentyn-Góral,Halina Wiśniewska,Juliusz Perkowski 한국식물병리학회 2015 Plant Pathology Journal Vol.31 No.3
Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars – for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.
Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442
Małgorzata Góral,Urszula Pankiewi,Monika Sujka,Radosław Kowalski,Dariusz Góral,Katarzyna Kozłowicz 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.1
Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 μg/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.
Özay Önöral,Sevcan Kurtulmus-Yilmaz,Dilem Toksoy,Oguz Ozan 대한치과보철학회 2023 The Journal of Advanced Prosthodontics Vol.15 No.6
Purpose. The study aimed to determine the influence of implant angulation on the trueness of multi-unit implant impressions taken through different techniques and strategies. Materials and Methods. As reference models, three partially edentulous mandibular models (Model 1: No angulation; Model 2: No angulation for #33, 15-degree distal angulation for #35 and #37; Model 3: No angulation for #33, 25-degree distal angulation for #35 and #37) were created by modifying the angulations of implant analogues. Using a lab scanner, these reference models were scanned. The obtained data were preserved and utilized as virtual references. Three intraoral scanning (IOS) strategies: IOS-Omnicam, ISO-Quadrant, and IOS-Consecutive, as well as two traaditional techniques: splinted open tray (OT) and closed tray (CT), were used to create impressions from each reference model. The best-fit alignment approach was used to sequentially superimpose the reference and test scan data. Computations and statistical analysis of angular (AD), linear (LD), and 3D deviations (RMS) were performed. Results. Model type, impression technique, as well as interaction factor, all demonstrated a significant influence on AD and LD values for all implant locations (P < .05). The Model 1 and SOT techniques displayed the lowest mean AD and LD values across all implant locations. When considering interaction factors, CT-Model 3 and SOT-Model 1 exhibited the highest and lowest mean AD and LD values, respectively. Model type, impression technique, and interaction factor all revealed significant effects on RMS values (P ≤ .001). CT-Model 3 and SOT-Model 1 presented the highest and lowest mean RMS values, respectively. Conclusion. Splinted-OT and IOS-Omnicam are recommended for multi-unit implant impressions to enhance trueness, potentially benefiting subsequent manufacturing stages.
Burcu Günal-Abduljalil,Özay Önöral,Salim Ongun 대한치과보철학회 2021 The Journal of Advanced Prosthodontics Vol.13 No.3
Purpose. This study aimed to assess the influence of various micromechanical surface conditioning (MSC) strategies with or without coupling agent (silane) application on the micro-shear bond strength (μSBS) of resin- matrix ceramics (RMCs). Materials and Methods. GC Cerasmart (GC), Lava Ultimate (LU), Vita Enamic (VE), Voco Grandio (VG), and Brilliant Crios (BC) were cut into 1.0-mmthick slices (n = 32 per RMC) and separated into four groups according to the MSC strategy applied: control-no conditioning (C), air-borne particle abrasion with aluminum oxide particles (APA), 2W- and 3W-Er,Cr:YSGG group coding is missing. The specimens in each group were further separated into silaneapplied and silane-free subgroups. Each specimen received two resin cement microtubules (n = 8 per subgroup). A shear force was applied to the adhesive interface through a universal test machine and μSBS values were measured. Data were statistically analyzed by using 3-way ANOVA and Tukey HSD test. Failure patterns were scrutinized under stereomicroscope. Results. RMC material type, MSC strategy, and silanization influenced the μSBS values (P <.05). In comparison to the control group, μSBS values increased after all other MSC strategies (P <.05) while the differences among these strategies were insignificant (P >.05). For control and APA, there were insignificant differences between RMCs (P >.05). The silanization decreased μSBS values of RMCs except for VE. Considerable declines were observed in GC and BC (P <.05). Conclusion. MSC strategies can enhance bond strength values at the RMC - cement interface. However, the choice of MSC strategy is dependent on RMC material type and each RMC can require a dedicated way of conditioning.
Fikret Sönmez,Hüdayim Başak,Ahmet Güral,Şehmus Baday 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.10
In this study, firstly all of Al-7075-T6 alloys were joined by the friction stir welding (FSW) method then the samples were divided into four groups. First group of samples were used directly. The second group of samples was burnished. Third group of samples were aged and the last group of samples was subjected to both re-aging and burnishing process. It was compared the tensile and bending strengths of all prepared specimens. After the FSW process, only the second aging process increased the tensile strength. There is no effect on the tensile strength of the burnishing process. The bending strength has improved only the re-aging process without burnishing operation or only burnishing without the second aging process. In the friction stir welded Al-7075-T6 materials, it was concluded not to have a significant effect on the mechanical properties of the burnishing process. However, the hardness values of the materials were increased by both the re-aging and the burnishing process.