Protective Effects of Verapamil against H2O2-Induced Apoptosis in Human Lens Epithelial Cells

( Zhuo Wang ),( Dan Wang ),( Yan Li ),( Xiuli Zhang ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Verapamil is used in the treatment of hypertension, angina pectoris, and atrial fibrillation. Recently, several studies have demonstrated that verapamil increased the optic nerve head blood flow and improved the retrobulbar circulation. All these show that verapamil is potentially useful for ophthalmic treatment. Thus, the aim of this study is to investigate whether verapamil could protect human lens epithelial cell (HLEC) from oxidative stress induced by H2O2 and the cellular mechanism underlying this protective function. The viability of HLEC was determined by the MTT assay and apoptotic cell death was analyzed by Hoechst 33258 staining. Moreover, Caspase-3 expression was detected by immunocytochemistry and flow cytometry analysis. We also detected Caspase-3 mRNA expression by reverse-transcription-polymerase chain reaction and the GSH content in cell culture. The results showed that oxidative stress produced significant cell apoptotic death and it was reduced by previous treatment with the verapamil. Verapamil was effective in reducing HLEC death mainly through reducing the expression level of apoptosis-related proteins, caspase-3, and increasing glutathione content. Therefore, it was suggested that verapamil was effective in reducing HLEC apoptosis induced by H2O2.

Identifying Differentially Expressed Genes and Screening Small Molecule Drugs for Lapatinib-resistance of Breast Cancer by a Bioinformatics Strategy

Zhuo, Wen-Lei,Zhang, Liang,Xie, Qi-Chao,Zhu, Bo,Chen, Zheng-Tang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: Lapatinib, a dual tyrosine kinase inhibitor that interrupts the epidermal growth factor receptor (EGFR) and HER2/neu pathways, has been indicated to have significant efficacy in treating HER2-positive breast cancer. However, acquired drug resistance has become a very serious clinical problem that hampers the use of this agent. In this study, we aimed to screen small molecule drugs that might reverse lapatinib-resistance of breast cancer by exploring differentially expressed genes (DEGs) via a bioinformatics method. Materials and Methods: We downloaded the gene expression profile of BT474-J4 (acquired lapatinib-resistant) and BT474 (lapatinib-sensitive) cell lines from the Gene Expression Omnibus (GEO) database and selected differentially expressed genes (DEGs) using dChip software. Then, gene ontology and pathway enrichment analyses were performed with the DAVID database. Finally, a connectivity map was utilized for predicting potential chemicals that reverse lapatinib-resistance. Results: A total of 1, 657 DEGs were obtained. These DEGs were enriched in 10 pathways, including cell cycling, regulation of actin cytoskeleton and focal adhesion associate examples. In addition, several small molecules were screened as the potential therapeutic agents capable of overcoming lapatinib-resistance. Conclusions: The results of our analysis provided a novel strategy for investigating the mechanism of lapatinib-resistance and identifying potential small molecule drugs for breast cancer treatment.

The Laser Swing Detector

Zhuo Zhou Hong 대한전자공학회 1991 International Conference on Electronics, Informati Vol.1 No.1

Antidumping case in the China's textile industry: A model building approach

Zhuo, Jun,Park, Yong H. Kyung Hee University Management Research Institute 2019 Asia pacific journal of business review Vol.3 No.2

Anti-dumping instruments among trading partners have been the subject of research by both academicians and practitioners. This study attempts to establish an early-warning model of anti-dumping against Chinese textile exporting companies, which have suffered from anti-dumping regulations and got arbitration awards. After reviewing theories of anti-dumping arbitration, early-warning and relationship marketing, the measuring items and relationship marketing model of Chinese textiles exporters are investigated. Empirical methods are selected based on early-warning theories of companies. Eighty percent of 156 valid questionnaires by surveys and interviews are used as training data via Binary-Logistic regression while the other twenty percent are validated in the model. As a result, a proper early-warning model has been established.

The laser swing detector

Zhuo zhou Hong 대한전자공학회 1991 ICEIC:International Conference on Electronics, Inf Vol.1991 No.8

Size-dependent fracture properties of cracked silicon nanofilms

Zhuo, X.R.,Beom, H.G. Elsevier 2015 Materials science & engineering. properties, micro Vol.636 No.-

<P><B>Abstract</B></P> <P>We evaluate the applicability of the critical energy release rate as a crack propagation criterion with a focus on the effect of the material size. Cracked silicon nanofilms of various thicknesses under uniaxial tensile loading are simulated to examine the influence of film thickness on the critical energy release rate. Tensile stress–strain curves in addition to the atomic configurations corresponding to different strain levels are presented to elucidate the deformation mechanism. The cracked silicon nanofilms subjected to mode I loading deform nonlinearly to some extent. The critical energy release rates are calculated by three approaches: a molecular statics simulation, the Griffith criterion, and linear elastic fracture mechanics. Results obtained from these three methods are compared with each other and the differences between them are discussed. The critical energy release rate may be capable of predicting crack propagation when the silicon nanofilms are thicker than approximately 18nm.</P>

Cluster analysis of city-level carbon mitigation in South Korea

Zhuo Li(이탁) 한국컴퓨터정보학회 2023 韓國컴퓨터情報學會論文誌 Vol.28 No.7

최근 지구온난화로 인한 폭염, 태풍, 폭설 등 기후변화를 급증하고 있다. 미국 뉴욕에서 개최된 제 25차 기후변화 당사국총회(COP25)에 따른 세계 각국은 탄소중립 달성하기 위한 협상을 진행했다. 도시는 경제발전뿐만 아니라 탄소중립 과정에서도 중요한 역할을 수행한다. 본 연구는 이산화탄소와 관계되는 경제요인 및 환경요인을 고려하여 엘보우 규칙 (Elbow method) 과 K-means 군집 알고리즘을 활용하여 한국 63개 도시의 탄소배출 현황을 분석하였다. 연구결과에 따른 한국 도시는 기술집약 도시, 경공업 도시, 미래 혁신도시, 중공업 도시, 서비스 집약도시 및 농촌, 가정생산집약도시로 구분될 수 있고 향후 시도별 탄소중립 목표를 실천하기 위해 구체적인 제안을 제시하였다. The phenomenon of climate change is deteriorating which increased heatwaves, typhoons and heavy snowfalls in recent years. Followed by the 25th United nations framework convention on climate change(COP25), the world countries have achieved a consensus on achieving carbon neutrality. City plays a crucial role in achieving carbon mitigation as well as economic development. Considering economic and environmental factors, we selected 63 cities in South Korea to analyze carbon emission situation by Elbow method and K-means clustering algorithm. The results reflected that cities in South Korea can be categorized into 6 clusters, which are technology-intensive cities, light-manufacturing intensive cities, central-innovation intensive cities, heavy-manufacturing intensive cities, service-intensive cities, rural and household-intensive cities. Specific suggestions are provided to improve city-level carbon mitigation development.

Size-dependent torsional deformation of silicon nanowires

Zhuo, Xiao Ru,Beom, Hyeon Gyu Elsevier 2018 Materials letters Vol.213 No.-

<P><B>Abstract</B></P> <P>The effect of size on the torsional deformation of [1 1 0]-oriented silicon nanowires is examined via molecular dynamics simulations. The plastic deformation mechanism of silicon nanowires depends on the nanowire diameter. Sub-5.7-nm-diameter silicon nanowires yield by means of a crystal-to-amorphous transition, which nucleates at the outer surface and propagates along the cross-section of the wires. On the other hand, perfect dislocations nucleate at the outer surface of wires whose diameter equals or exceeds 7.7 nm, initiating plastic deformation. These dislocations have a Burgers vector of 1/2 ⟨1 1 0⟩ and slip along the {1 1 1} planes. These findings may enhance our understanding of plasticity of silicon nanowires.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Torsional deformation of [1 1 0]-oriented silicon nanowires is size-dependent. </LI> <LI> Sub-5.7-nm-diameter silicon nanowires yield by a crystal-to-amorphous transition. </LI> <LI> Coaxial dislocations form in nanowires whose diameter equals or exceeds 7.7 nm. </LI> </UL> </P>

An Unconventional Outer-to-Inner Synthesis Strategy for Core (Au)-Shell Nanostructures with Photoelectrochemical Enhancement

Zhuo Zhang,Chen Bin,용기중 한국진공학회 2018 한국진공학회 학술발표회초록집 Vol.2018 No.2

Effects on Range of motion of Neck joint through Five-Animals Micro-Exercises

ZHUO YAN,XU Jiang,HE LI,ZHU WANLING 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.1

This research was aiming to provide new-styles sport exercises for white and blue-collar workers and prevented people from suffering occupational neck joint illnesses, released the pains from joints of neck and improving the ranges of motions of neck joints throughout the observations that was investigated among staffs who were from a research institute. This research carried out the suggested intervention called Five-Animals Micro-Exercises and then observe its effects that indexes which reflected the flexion, extension, lateral flexion, rotation and pain degree of joint neck would change. There were 320 staffs were chosen from all working staffs who employed by the institute as individual subjects. These 320 staffs did the Five-Animal Micro-Exercise and lasted for 20 weeks during the intervention period. In the progress of intervention, all subjects were divided into groups according to their working locations and organized to do the exercise which lasted for 10 to 15 minutes then exercise frequency was required at least 2 times a day and no more less than 5 days a week. Groups leaders would be elected among the groups and needed to record sport situations of group members. All group members could decide to join the grouped exercise or did the sport alone when they are convenient. The results of intervention would be collected when beginning and ending of this experiment, which is in order to determine the indexes flexion, extension, lateroflexion, rotation, pain degree of joint neck and body attitude assessment. Moreover, 177 subjects who showed higher compliance and reached the required standard of exercising were selected from weekly sport situations subjects’ feedback. The neck joint range of motion had significant changes after intervention, include flexion, extension, lateroflexion, and rotation(p<.05).Besides,, the pain degree and points of body attitude assessment of subjects stated dramatically variation (p<.05).The Five-Animal Micro-Exercise contributes on enlarging the degrees of flexion, extension, lateroflexion, rotation, and pain degree of joint neck are is helpful on improving joint neck degree. It can also relief joint pain, relax muscle, upraises endurance of muscles and increases the coordination of body, which are good for preventing cervical spondylopathy, and help people to maintain their body cures.