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TANNylated-AAV for Enhanced Gene Delivery
Zheng Rong LAU,Ginah HAN,Jae-Hyung JANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Adeno-associated virus (AAV) has received tremendous attention for developments in gene therapy applications, leading to several approvals of AAV-based gene therapy products such as Glybera, Zolgensma, and Luxturna. However, much can still be done in optimizing the efficacy of AAV as a delivery vehicle for gene therapy. Among the many drawbacks of AAV as a delivery vector, the problem of its relatively low genome packaging capacity of only about 4.7 kb is especially detrimental, resulting in cases of which transgene need to be fragmented and packaged separately prior to administration. Herein, we demonstrate that through rather simple tannic acid treatment onto AAV to form tannic acid-treated AAV (namely TANNylated-AAV), the transduction efficiency is enhanced. Tannic acid is found to have high affinity to proline-rich extracellular matrices (ECM) and collagens, which leads us to hypothesize that TANNylated-AAV can allow for enhanced targeted delivery. We utilized hypoxic ischemic (HIE) brain injury mouse model to observe enhanced AAV transduction towards ECM-rich reactive astrocytes populated glial scar region of infarct area.
Colorectal Cancer Incidence and Mortality in China, 2010
Zheng, Zhao-Xu,Zheng, Rong-Shou,Zhang, Si-Wei,Chen, Wan-Qing Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19
Background: The National Central Cancer Registry of China (NCCR) affiliated to the Bureau of Disease Control, National Health and Family Planning Commission of China is responsible for cancer surveillance in the entire country. Cancer registration data from each local registry located in each province are collected by NCCR annually to be analyzed and published to provide useful information for policy makers and cancer researchers. Materials and Methods: Until 1st June, 2013, 219 population-based cancer registries submitted data of 2010 to the National Central Cancer Registry of China covering about 207,229,403 population, and 145 cancer registries were selected after quality evaluation for this study. Colorectal cancer cases were selected from the database according to ICD-10 coded as "C18-C20". We calculated the crude incidence and mortality rates by sex, age groups and location (urban/rural). The China population in 2000 and Segi's population were used as standardized populations for the calculation of age-standardized rates. The 6th National Population Census data of China was used to combined with the cancer registries' data to estimate the colorectal cancer burden in China in 2010. Results: Colorectal cancer was the sixth most common cancer in China. It was estimated that there were 274,841 new cases diagnosed in 2010 (157,355 in males and 117,486 in females), with the crude incidence rate of 20.1/100,000, highest in males in urban areas. Age-standardized rates by China standard population of 2000 (ASRcn) and World standard population (Segi's population, ASRwld) for incidence were 16.1/100,000 and 15.9/100,000 respectively. There were 132,110 cases estimated to have died from colorectal cancer in China in 2010 (76,646 men and 55,464 women) with the crude mortality rate of 10.1/100,000. The ASRcn and ASRwld for mortality were 7.55/100,000 and 7.44/100,000 respectively, higher in males and urban areas than in females and rural areas. The incidence and mortality rates increased with age, reaching peaksin the 80-84 year old, and oldest age groups, respectively. Conclusions: Colorectal cancer is one of the most common incident cancers and cause of cancer death in China. Primary and secondary prevention, with attention to a health lifestyle, physical activity and screening should be enhanced in the general population.
Multitap coherence-free photonic RF filter employing semiconductor optical amplifier
Zheng, Rong,Alameh, Kamal,Lee, Yong Tak,Wang, Zhenglin Wiley Subscription Services, Inc., A Wiley Company 2007 MICROWAVE AND OPTICAL TECHNOLOGY LETTERS Vol.49 No.5
<P>A novel multitap coherence-free photonic bandpass RF filter utilizing the cross-gain modulation property of the semiconductor optical amplifier to convert a coherent RF-modulated laser carrier to a low-coherence light that is sliced and delayed to realize a stable transversal RF filter. An 8-tap photonic RF bandpass filter operating at 2.25 GHz with more than 15 dB rejection is experimentally demonstrated. © 2007 Wiley Periodicals, Inc. Microwave Opt Technol Lett 49: 1103–1106, 2007; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.22352</P>
Modeling of flow-induced crystallization of colored polypropylene in injection molding
Rong Zheng,R. I. Tanner,D. Lee Wo,Xi-jun Fan,C. Hadinata,F.S. Costa,P.K. Kennedy,P. Zhu,G. Edward 한국유변학회 2010 Korea-Australia rheology journal Vol.22 No.3
We present a study on flow-induced crystallization of an injection-molded isotactic polypropylene (iPP) mixed with colorant additives. Two types of blue colorants were used in the study: one is the ultramarine Blue composed of Sodium Alumino Sulpho Silicate (UB) and the other is the PV Fast Blue composed of Cu-Phthalocyanine (CuPc). The CuPc colorant exhibits increased nucleation of both quiescent and flow-induced crystallization, and results in more oriented microstructures, causing a high degree of anisotropy in material properties and shrinkage of the injection molded parts. In contrast, the nucleating effect of the UB colorant is negligible under the quiescent condition, but becomes notable in the case of short-term shear treatment at high shear rates. The UB colorant does not significantly increase the degree of anisotropy as compared with the virgin iPP. A flow-induced crystallization kinetic model is used in the numerical simulation for the injection molding process, and predicted the colorant effects as observed in experiments.
Zheng, Shu-Rong,Guo, Gui-Long,Zhai, Qi,Zou, Zhang-Yong,Zhang, Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.4
Diverse studies have shown that miR-155 is overexpressed in different tumor types. However, the precise molecular mechanism of the ectopic expression of miR-155 in breast cancer is still poorly understood. To further explore the role of miR-155 in breast tumorigenesis, we here assessed the influence of miR-155 antisense oligonucleotide (miR-155 ASO) on MDA-MB-157 cell viability and apoptosis in vitro. Furthermore, the effects of inhibitory effects of miR-155 on the growth of xenograft tumors in vivo were determined with performance of immunohistochemistry to detect expression of caspase-3, a pivotal apoptosis regulatory factor, in xenografts. Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155 expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared with that in cells transfected with a negative control. After being transfected with miR-155 ASO, the viability of MDA-MB-157 cells was reduced greatly (P<0.05) and the number of apoptotic cells was increased significantly. Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo and significantly increased the expression of caspase-3. Taken together, our study revealed that miR-155 ASO can induce cell apoptosis and inhibit cell proliferation in vitro. Moreover, miR-155 ASO could significantly repress tumor growth in vivo, presumably by inducing apoptosis via caspase-3 up-regulation. These findings provide experimental evidence for using miR-155 as a therapeutic target of breast carcinoma.
A multi-strategy enhanced African vultures optimization algorithm for global optimization problems
Zheng Rong,Hussien Abdelazim G,Qaddoura Raneem,Jia Heming,Abualigah Laith,Wang Shuang,Saber Abeer 한국CDE학회 2023 Journal of computational design and engineering Vol.10 No.1
The African vultures optimization algorithm (AVOA) is a recently proposed metaheuristic inspired by the African vultures’ behaviors. Though the basic AVOA performs very well for most optimization problems, it still suffers from the shortcomings of slow convergence rate and local optimal stagnation when solving complex optimization tasks. Therefore, this study introduces a modified version named enhanced AVOA (EAVOA). The proposed EAVOA uses three different techniques namely representative vulture selection strategy, rotating flight strategy, and selecting accumulation mechanism, respectively, which are developed based on the basic AVOA. The representative vulture selection strategy strikes a good balance between global and local searches. The rotating flight strategy and selecting accumulation mechanism are utilized to improve the quality of the solution. The performance of EAVOA is validated on 23 classical benchmark functions with various types and dimensions and compared to those of nine other state-of-the-art methods according to numerical results and convergence curves. In addition, three real-world engineering design optimization problems are adopted to evaluate the practical applicability of EAVOA. Furthermore, EAVOA has been applied to classify multi-layer perception using XOR and cancer datasets. The experimental results clearly show that the EAVOA has superiority over other methods.
Applying Artificial Neural Network to Fault Diagnosis of Analogue Circuit
Yang, ZhEng Rong,Musgrave, G. 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1
In this paper, we have applied artificial neural networks to fault diagnosis of analogue circuits. The experiments presented have proved that the artificial neural network can be used both for catastrophic faults and parametric faults. We have applied three artificial neural network, models to the fault diagnosis of DC, frequency, parametric, and steady state response. Two important techniques are introduced for the selection of artificial neural network models. These two techniques are: pattern distinction and quantity factor graph.