Voluntary running depreciates the requirement of Ca²⁺-stimulated cAMP signaling in synaptic potentiation and memory formation

Zheng, Fei,Zhang, Ming,Ding, Qi,Sethna, Ferzin,Yan, Lily,Moon, Changjong,Yang, Miyoung,Wang, Hongbing Cold Spring Harbor Laboratory Press 2016 Learning & Memory Vol.23 No.8

<P>Mental health and cognitive functions are influenced by both genetic and environmental factors. Although having active lifestyle with physical exercise improves learning and memory, how it interacts with the specific key molecular regulators of synaptic plasticity is largely unknown. Here, we examined the effects of voluntary running on long-term potentiation (LTP) and memory formation in mice lacking type 1 adenylyl cyclase (AC1), a neurospecific synaptic enzyme that contributes to Ca2+-stimulated cAMP production. Following 1 mo of voluntary running-wheel exercise, the impaired LTP and object recognition memory in AC1 knockout (KO) mice were significantly attenuated. Running up-regulated exon II mRNA level of BDNF (brain-derived neurotrophic factor), though it failed to increase exon I and IV mRNAs in the hippocampus of AC1 KO mice. Intrahippocampal infusion of recombinant BDNF was sufficient to rescue LTP and object recognition memory defects in AC1 KO mice. Therefore, voluntary running and exogenous BDNF application overcome the defective Ca2+-stimulated cAMP signaling. Our results also demonstrate that alteration in Ca2+-stimulated cAMP can affect the molecular outcome of physical exercise.</P>

Measurement of 3D Curve Structures By Using One Hand-held Digital Camera

Zheng Jian-dong,Zhang Li-yan,Wang Hong-tao,Ding Zhi-an (사)한국CDE학회 2007 한국CAD/CAM학회 국제학술발표 논문집 Vol.2007 No.8

Icariside II Promotes the Differentiation of Adipose Tissue-Derived Stem Cells to Schwann Cells to Preserve Erectile Function after Cavernous Nerve Injury

Zheng, Tao,Zhang, Tian-biao,Wang, Chao-liang,Zhang, Wei-xing,Jia, Dong-hui,Yang, Fan,Sun, Yang-yang,Ding, Xiao-ju,Wang, Rui Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6

Icariside II (ICA II) is used in erectile dysfunction treatment. Adipose tissue-derived stem cells (ADSCs) are efficient at improving erectile function. This study aimed to explore the action mechanism of ADSCs in improving erectile function. ADSCs were isolated from the adipose tissues of rats. Cell proliferation was determined using the Cell Counting Kit-8 (CCK-8) assay. The expressions of mRNA and protein were determined separately through qRT-PCR and western blot. The endogenous expressions of related genes were regulated using recombinant plasmids and cell transfection. A Dual-Luciferase Reporter Assay was performed to determine the interaction between miR-34a and STAT3. Rat models with bilateral cavernous nerve injuries (BCNIs) were used to assess erectile function through the detection of mean arterial pressure (MAP) and intracavernosal pressure (ICP). ICA II promoted ADSCs' proliferation and differentiation to Schwann cells (SCs) through the inhibition of miR-34a. Suppressed miR-34a promoted the differentiation of ADSCs to SCs by upregulating STAT3. ICA II promoted the differentiation of ADSCs to SCs through the miR-34a/STAT3 pathway. The combination of ICA II and ADSCs preserved the erectile function of the BCNI model rats. ADSCs treated with ICA II markedly preserved the erectile function of the BCNI model rats, which was reversed through miR-34a overexpression. ICA II promotes the differentiation of ADSCs to SCs through the miR34a/STAT3 pathway, contributing to erectile function preservation after the occurrence of a cavernous nerve injury.

β-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils

Ding Li,Changsen Bai,Qing Zhang,Zheng Li,Di Shao,Xichuan Li 한국미생물학회 2019 The journal of microbiology Vol.57 No.4

Since molecular genotyping has been established for the Candida species, studies have found that a single Candida strain (endemic strain) can persist over a long period of time and results in the spread of nosocomial invasive candidiasis without general characteristics of horizontal transmissions. Our previous study also found the existence of endemic strains in a cancer center in Tianjin, China. In the current study, we performed further investigation on endemic and non-endemic Candida albicans strains, with the aim of explaining the higher morbidity of endemic strains. In an in vivo experiment, mice infected with endemic strains showed significantly shorter survival time and higher kidney fungal burdens compared to mice infected with non-endemic strains. In an in vitro experiment, the killing percentage of neutrophils to endemic strains was significantly lower than that to non-endemic strains, which is positively linked to the ratio of LC3B-II/I in neutrophils. An immunofluorescence assay showed more β-1,3-glucan exposure on the cell walls of nonendemic strains compared to endemic strains. After blocking the β-glucan receptor (CR3) or inhibiting downstream kinase (SYK) in neutrophils, the killing percent to C. albicans (regardless of endemic and non-endemic strains) and the ratio of LC3B-II/I of neutrophils were significantly decreased. These data suggested that the killing capability of neutrophils to C. albicans was monitored by β-1,3-glucan via CR3/SYK pathway-dependent LC3B-II accumulation and provided an explanation for the variable killing capability of neutrophils to different strains of C. albicans, which would be beneficial in improving infection control and therapeutic strategies for invasive candidiasis.

Promoting N2 electroreduction to ammonia by fluorine-terminating Ti3C2Tx MXene

Ding Yu,Zhang Junbo,Guan Anxiang,Wang Qihao,Li Si,Al-Enizi Abdullah M.,Qian Linping,Zhang Lijuan,Zheng Gengfeng 나노기술연구협의회 2021 Nano Convergence Vol.8 No.14

Two-dimensional MXene-based materials are potential of presenting unique catalytic performances of electrocatalytic reactions. The surface functionalization of MXene-based catalysts is attractive for developing efficient electrocatalysts toward nitrogen reduction reaction. Herein, we reported a Ti 3 C 2 T x MXene with a medium density of surface functionalized fluorine terminal groups, as an excellent N 2 reduction reaction electrocatalyst with enhanced adsorption and activation of N 2 . The Ti 3 C 2 T x MXene catalyst showed a production rate of ammonia as 2.81 × 10 –5 μmol·s −1 ·cm −2 , corresponding to a partial current density of 18.3 μA·cm −2 and a Faradic efficiency of 7.4% at − 0.7 V versus reversible hydrogen electrode in aqueous solutions at ambient conditions, substantially exceeding similar Ti 3 C 2 T x MXene catalysts but with higher or lower densities of surface fluorine terminal groups. Our work suggests the capability of developing surface functionalization toolkit for enhancing electrochemical catalytic activities of two-dimensional MXene-based materials.

Rapid Detection of Trace 1,4-Dichlorobenzene Using Laser Mass Spectrometry

Ding, Lei,Ma, Jing,Zheng, Haiyang,Fang, Li,Zhang, Weijun,Kim, Duk-Hyeon,Cha, Hyung-Ki Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.9

The 1+1 two-photon Resonant Enhanced Multiphoton Ionization (REMPI) spectra of 1,4-dichlorobenzene was obtained from 240 nm through to 250 nm on a laser mass spectrometer. Special care was taken to build up a heatable sample inlet system suitable for detecting a trace semi-volatile organic compound and reducing the memory effort on the inner wall of the inlet system. The detection limits of 1,4-dichlorobenzene in ppbV/V concentration range at certain wavelengths are presented.

Surgical Outcomes of Symptomatic Intramedullary Spinal Cord Cavernous Malformations: Analysis of Consecutive Cases in a Single Center

Zheng Cai,Xinjie Hong,Wei Dai,Zhengwei Zhang,Qiang Liang,Xuehua Ding,Wei Sun 대한척추신경외과학회 2023 Neurospine Vol.20 No.3

Objective: Intramedullary spinal cavernous malformations (ISCMs) are rare vascular lesions of the spinal cord with unclear natural history and controversy over treatment. This study aimed to report a series of symptomatic ISCMs underwent microsurgical management to illustrate the natural history, clinical presentation, and surgical outcomes and to evaluate factors associated with hemorrhage events and neurological prognosis. Methods: This single-center retrospective study included 29 consecutive patients with whose demographic, symptomology, imaging, neurological, and surgical data were collected. The risk for hemorrhage events and factors affecting surgical outcomes were retrospectively analyzed. Results: There were 12 female (41.4%) and 17 male patients (58.6%), with an average age of 45.2 years (range, 17–69 years). The mean size of the lesion was 9.7 mm (range, 3–20 mm). Most patients had a bowel or/and bladder dysfunction symptom (n = 11, 37.9%), followed by sensory deficits (n = 5, 17.2%), gait disturbance (n = 5, 17.2%), pain (n = 4, 13.8%), and weakness (n = 4, 13.8%), most (n = 15, 51.7%) with a chronic onset. All patients received total resection without rehemorrhages after surgical resection in follow-up. Sixty-five point five percent patients (n = 19) improved, 13.8% (n = 4) remained stable, 20.7% (n = 6) got worsen. The overall annual hemorrhage risk was 2.1% per patient-year. A total of 27 hemorrhages occurred in the 18 patients, of which rehemorrhage rate increased to 50.0% (n = 9) with a previous history of hemorrhage. Patients with smaller lesion sizes were more likely to have hemorrhage or rehemorrhage events (p = 0.008). Recurrent hemorrhage of the lesions was a risk factor for neurological outcomes (p = 0.016). Conclusion: The risk of rehemorrhage was significantly increased in symptomatic ISCM patients with a previous history of hemorrhage. Rehemorrhage was a risk factor for neurological outcomes. Patients can benefit from microsurgical treatment to avoid rehemorrhage and further neurological deterioration.

Overexpression of CXCL2 inhibits cell proliferation and promotes apoptosis in hepatocellular carcinoma

( Jun Ding ),( Kangdi Xu ),( Jie Zhang ),( Bingyi Lin ),( Yubo Wang ),( Shengyong Yin ),( Haiyang Xie ),( Lin Zhou ),( Shusen Zheng ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.12

C-X-C motif chemokine ligand 2 (CXCL2) is a small secreted protein that exhibits a structure similar to the proangiogenic subgroup of the CXC chemokine family. Recently, accumulating evidence suggests that chemokines play a pivotal role in cancer progression and carcinogenesis. We examined the expression levels of 7 types of ELR+ CXCLs messenger RNA (mRNA) in 264 clinical samples. We found that CXCL2 expression was stably down-regulated in 94% of hepatocellular carcinoma (HCC) specimens compared with paired adjacent normal liver tissues and some HCC cell lines. Moreover, CXCL2 overexpression profoundly attenuated HCC cell proliferation and growth and induced apoptosis in vitro. In animal studies, we found that overexpressing CXCL2 by lentivirus also apparently inhibited the size and weight of subcutaneous tumours in nude mice. Furthermore, we demonstrated that CXCL2 induced HCC cell apoptosis via both nuclear and mitochondrial apoptosis pathways. Our results indicate that CXCL2 negatively regulates the cell cycle in HCC cells via the ERK1/2 signalling pathway. These results provide new insights into HCC and may ultimately lead to the discovery of innovative therapeutic approaches of HCC. [BMB Reports 2018; 51(12): 630-635]

Effects of dietary yeast beta-1,3-1,6-glucan on growth performance, intestinal morphology and chosen immunity parameters changes in Haidong chicks

Baoan Ding,Juanshan Zheng,Xiezhong Wang,Licheng Zhang,De Sun,Quanhu Xing,Andrea Pirone,Baldassare Fronte 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.10

Objective: This study investigated the effects of 1,3-1,6 beta-glucan added to the diet of Haidong chicks reared under hypoxic conditions, to ascertain the growth performances, immunity and intestinal morphology changes. Methods: A total of 750 chicks were divided into five groups and fed diets containing 0.5 g/kg, 1.0 g/kg, and 2.0 g/kg 1,3-1,6 beta-glucan from yeast (G1, G2, G3, respectively), 0.2 g/kg Taylor rhizomorph and a control feed. Results: The body weight and body weight gain were higher in chicks fed 1,3-1,6 beta-glucan and Taylor rhizomorph than in control group. Feed conversion ratio significantly differed for G2 and G3 groups in comparison to control group. The relative weight of bursa was higher in G1, G2, and G3 groups. The white blood cells and lymphocytes were significantly increased in groups fed 1,3-1,6 beta-glucan. The immunoglobulin G of serum peak appeared in the G3 group. The villous height of the duodenum was higher in 1,3-1,6 beta-glucan feed groups. In the jejunum, the villous height was higher in G2 and G3 groups and crypt depth for all the groups fed β-glucan. At ileum level the villous height and crypt depth was higher for groups G1, G2, and G3. Conclusion: The growth performance of Haidong chicks is improved when 10 and 20 g/kg 1,3-1,6 beta-glucan is included in the diet; hence, it is suggested that 1,3-1,6 beta-glucan be included in poultry diet to reduce and replace the use of antibiotics.

Pulmonary IFN-γ Causes Lymphocytic Inflammation and Cough Hypersensitivity by Increasing the Number of IFN-γ-Secreting T Lymphocytes

Deng Zheng,Ding Wenbin,Li Fengying,Shen Shuirong,Huang Chuqin,Lai Kefang 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.6

Purpose: Respiratory viral infection increases the number of lung-resident T lymphocytes, which enhance cough sensitivity by producing interferon-γ (IFN-γ). It is poorly understood why IFN-γ-secreting T lymphocytes persist for a long time when the respiratory viruses have been removed. Methods: Repeated pulmonary administration of IFN-γ and intraperitoneal injection with different inhibitors were used to study the effects of pulmonary IFN-γ in mice and guinea pigs. Results: IFN-γ administration caused the increasing of IFN-γ-secreting T lymphocytes in both lung and blood, followed by the elevated physiological level of IFN-γ in the lung, the airway inflammation and the airway epithelial damage. IFN-γ administration also enhanced the cough sensitivity of guinea pigs. IFN-γ activated the STAT1 and extracellular signal-regulated kinase (ERK) pathways in lung tissues, released IFN-γ-inducible protein 10 (IP-10), and resulted in F-actin accumulation in lung-resident lymphocytes. The CXC chemokine receptor 3 (CXCR3) inhibitor potently suppressed all the IFN-γ-induced inflammatory changes. The STAT1 inhibitor mitigated IFN-γ-secreting T lymphocytes infiltration by inhibiting T lymphocytes proliferation. F-actin accumulation and the ERK1/2 pathway contributed to pulmonary IFN-γ-induced augmentation of the airway inflammation and increasing of IFN-γ-secreting T lymphocytes in blood. Conclusions: High physiological levels of IFN-γ in the lung may cause pulmonary lymphocytic inflammation and cough hypersensitivity by increasing the number of IFN-γ-secreting T lymphocytes through the IP-10 and CXCR3 pathways.