Expression of the Transgene is Consistently Inherited to High Numbers of Generations and Independent on Its Source

Zhen-Yu Zheng,Keon Bong Oh,Deog-Bon Koo,Yong-Mahn Han,Kyung-Kwang Lee 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.1

Most studies on transgenic bioreactors have focused on expression levels of interest genes. In this study we examined whether transgenic bioreactors would inherit expression level of the Oansgene to long-term generations independently of transgene sources. We employed three transgenic mice, which were separately reported, carrying different transgenes and copy numbers, 27 kb of hLF and 22 kb of hIL-10 genomic sequences, and 1.3 kb of hTPO cDNA, respectively. Three females of the transgenic lineages crossbred with a wild-type male up to 20 generations to test transgenic frequencies of their progenies and to determine expression levels of the transgenes. Ultimately, transmission rates of kLF, hIL-10, and hTPO were 64.3+-7.0, 59.3+-9.8, and 56.1+-9.7, respectively, appeared following Mendelian pattern of inheritance. Notably, we found that levels of expressions of hLF, hIL-10, and hTPO in milk were sustained to high numbers of generations. No transgene silencing of expression was observed in every generations of all transgenic mice. In conclusion, we suggest that once established animal bioreactors could consistently transmit the transgene to continual generations, without loss of expressional activity, independently of transgene sources.

Anti-Inflammatory Effects of the 70 kDa Heat Shock Protein in Experimental Stroke

Zheng, Zhen,Kim, Jong Youl,Ma, Hualong,Lee, Jong Eun,Yenari, Midori A SAGE Publications 2008 Journal of cerebral blood flow and metabolism Vol.28 No.1

<P> The 70-kDa heat shock protein (Hsp70) is involved in protecting the brain from a variety of insults including stroke. Although the mechanism has been largely considered to be because of its chaperone functions, recent work indicates that Hsp70 also modulates inflammatory responses. To explore how and whether Hsp70 regulate immune responses in brain ischemia, mice overexpressing Hsp70 (Hsp Tg) were subjected to 2 h middle cerebral artery occlusion, followed by 24 h reperfusion. Parallel experiments were performed using a brain inflammation model. Hsp Tg microglia cocultured with astrocytes were used to evaluate the direct effects of Hsp70 on cytotoxicity of mcrigolia. Compared with wild-type (Wt) littermates, Hsp Tg mice showed decreased infarct size and improved neurological deficits. The number of activated microglia/macrophages were also reduced in ischemic brains of Hsp Tg mice. Similar observations were made in a model of brain inflammation that does not result in brain cell death. Overexpression of Hsp70 in microglia completely prevented microglia-induced cytotoxicity to astrocytes. Activation of the inflammatory transcription factor, nuclear factor-κB (NF-κB) was inhibited significantly in Hsp Tg mice and microglia. This was associated with decreased phosphorylation of NF-κB inhibitor protein, IκBα, and decreased expression of several NFκB-regulated genes. Co-immunoprecipitation studies revealed an interaction of Hsp70 with NF-κB and IκBα, but not with IkB kinase, IKKγ, suggesting that Hsp70 binds to the NF-κB:IκB complex preventing IκB phosphorylation by IKK. The findings of the present work establish an anti-inflammatory role for Hsp70 in the context of brain ischemia as a novel mechanism of protection. </P>

Expression of the Transgene is Consistently Inherited to High Numbers of Generations and Independent on Its Source

Zheng, Zhen-Yu,Oh, Keon-Bong,Koo, Deog-Bon,Han, Yong-Mahn,Lee, Kyung-Kwang The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.1

Most studies on transgenic bioreactors have focused on expression levels of interest genes. In this study we examined whether transgenic bioreactors would inherit expression level of the Oansgene to long-term generations independently of transgene sources. We employed three transgenic mice, which were separately reported, carrying different transgenes and copy numbers, 27 kb of hLF and 22 kb of hIL-10 genomic sequences, and 1.3 kb of hTPO cDNA, respectively. Three females of the transgenic lineages crossbred with a wild-type male up to 20 generations to test transgenic frequencies of their progenies and to determine expression levels of the transgenes. Ultimately, transmission rates of kLF, hIL-10, and hTPO were $64.3{\pm}7.0$, $59.3{\pm}9.8$, and $56.1{\pm}9.7$, respectively, appeared following Mendelian pattern of inheritance. Notably, we found that levels of expressions of hLF, hIL-10, and hTPO in milk were sustained to high numbers of generations. No transgene silencing of expression was observed in every generations of all transgenic mice. In conclusion, we suggest that once established animal bioreactors could consistently transmit the transgene to continual generations, without loss of expressional activity, independently of transgene sources.

Case Report : Pancreatic Panniculitis Associated with Acinar Cell Carcinoma of the Pancreas: A Case Report

( Zhen Jiang Zheng ),( Jun Gong ),( Guang Ming Xiang ),( Gang Mai ),( Xu Bao Liu ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.2

Pancreatic panniculitis is a rare type of disorder associated with pancreatic diseases. We describe here a case of 54-year-old man who was admitted to the Department of Dermatology with the diagnosis of erythema nodosum. The patient presented with a 9-month history of painful erythematous nodules on the extremities, joint pain and swelling, and weight loss. A highly elevated level of pancreatic lipase was found on the laboratory examinations. The biopsy specimens from the skin lesions showed subcutaneous fat necrosis. Abdominal computed tomography (CT) revealed a large mass with central necrosis in the body and tail of the pancreas. Distal pancreatectomy, splenectomy and partial transverse colectomy were successfully performed on day 17 of the hospitalization. The histopathologic findings supported the diagnosis of acinar cell carcinoma of the pancreas (ACCP). Postoperatively, the level of serum lipase returned to normal, and the skin lesions and joint manifestations gradually regressed. However, the swelling did not significantly resolve in the left knee. In view of the non-specific clinical presentation of this disease, clinicians should be alert and have a high index of suspicion for pancreatic panniculitis. (Ann Dermatol 23(2) 225∼228, 2011)

Adsorption of Lead(II) from Aqueous Solution by Two Modified Porous Chelating Resins Based on (3-Mercaptopropyl) Trimethoxysilane

Zhen-Zheng Wang,Hong-Dong Duan,Xia Meng,Yi-Fan Zhang,Hui-Min Qi 한국고분자학회 2016 Macromolecular Research Vol.24 No.9

Two novel types of thiol-based chelating resins were fabricated by modifying poly(2-hydroxyethyl methacrylate- co-glycerol dimethacrylate) (P(HEMA-GDMA)) and poly(2-hydroxyethyl methacrylate-co-ethylene glycol dimethacrylate) (P(HEMA-EGDMA)) with (3-mercaptopropyl) trimethoxysilane(MPS), and used to adsorb lead(II) from aqueous solution. The effects of initial lead(II) concentrations, solution pH, coexisting ions on adsorption of lead(II) were investigated. After modifying, the product P(HEMA-GDMA)-SH has excellent adsorption capacity (118.56 mg/g) for lead(II) at initial lead(II) concentration of 200 mg/L. The resins could easily be regenerated and were reused 6 times without significant loss of adsorption capacity. Besides, the presence of coexisting ions did not markedly influence the adsorption capacities of the resins. The results indicate that the functional thiol-based resins are efficient adsorbents for the removal of toxic lead(II) ions in water treatment.

Soil Conditioning Tests on Sandy and Cobbly Soil for Shield Tunneling

Zheng Zhen,Xinsheng Ge,Jun Zhang 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.4

Soil conditioning is an important stage in shield tunnel construction that can effectively improve muck flow plasticity, reduce cutter head wear and improve tunneling efficiency,especially in sandy and cobbly soils. The aim of this paper is to obtain the optimal ratio of residue modifiers in sandy and cobbly soil and to improve the equipment used in residue-related shield machines. Residual soil in a sandy pebble layer was selected, and particles with sizes over 50 mm were removed to form soil samples. Through laboratory tests, the optimal ratio of the modifier was obtained, and the shield tunneling machine equipment was optimized. The research results for the laboratory tests and equipment optimization were verified and adjusted appropriately through field tunneling tests. The following results were obtained. 1) A 3% foaming agent C was selected for the soil conditioning tests. The final mass ratio of the bentonite mixed solution of water, bentonite, soda ash (Na2CO3), and carboxymethylcellulose (CMC) was 6:1:0.028:0.035. 2) Improving earth pressure balance (EPB) shield machine equipment can improve the effects of soil conditioning. 3) Compared with the volume of residual soil, the optimal ratio of soil conditioning in sandy and cobbly layer was 13% water, 4 – 5% bentonite mixed solution and 12 – 15% foam. In addition, the maximum ratio shall be taken for the strata with poor grain composition. These results can be used to provide excavation guidelines for using an EPB machine under these soil conditions.

Acupuncture in Australia: regulation, education, practice, and research

Zhen Zheng 한국한의학연구원 2014 Integrative Medicine Research Vol.3 No.3

Acupuncture was introduced to Australia as early as in the 1880s, and is a form of complementary and alternative medicine in this country. In the past 2 decades since the 1990s, acupuncture has experienced a rapid growth. Today, nearly 4000 acupuncturists are registered with the Chinese Medicine Board of Australia. “Acupuncturist,” “Oriental medicine practitioner,” and “Chinese medicine practitioners” are protected titles for registered acupuncturists. A bachelor's degree of 4 years in related fields is the minimal requirement for registration in Australia. Three public universities and three major private colleges offer nine undergraduate and three postgraduate programs that are approved by the Chinese Medicine Board of Australia. Those three universities also offer Master-degree and Doctor of Philosophy programs. Acupuncture is well accepted by the Australians, with 10% having received this treatment and 80% general medical practitioners referring their patients to acupuncture service. All private health insurance schemes provide rebates to patients receiving acupuncture treatment, and third-party payment is also available in six of eight Australian states and territories. Research output in acupuncture has increased greatly since 2000. A majority of research focuses on acupuncture and Tai Chi as treatment modalities, and mainly investigates their mechanism of action, associated pain, and gynecological and respiratory conditions. The future direction of acupuncture in Australia is to introduce this medicine in hospitals and gain access to the medical benefit scheme so that acupuncture can be accessed by a wider community, in particular those who come from a disadvantaged background. In conclusion, improved education, regulation, and research of acupuncture in Australia put this country in a leading position among Western countries with respect to acupuncture services.

Being Adaptive to Pain Enhances Sham Acupuncture Analgesia: A Crossover Healthy Human Study

Zhen Zheng,Dawn Wong Lit Wan,Lars Arendt-Nielsen,Dong Y. Yao,Genevieve Iversen,Charlie C. Xue,Kelun Wang 사단법인약침학회 2017 Journal of Acupuncture & Meridian Studies Vol.10 No.6

We have reported a model that distinguishes pain adaptive individuals (PA) from those who are pain non-adaptive (PNA). The present randomised, cross-over, participant-assessor blinded study aimed to determine the impact of pain adaptability on individuals’ response to real and sham acupuncture. Healthy volunteers (nine PA and 13 PNA) were randomly allocated to receive real and sham acupuncture on the left hand and forearm in two separate acupuncture sessions. Pressure pain thresholds (PPTs) were measured at bilateral forearms and right leg before, immediately after and 20 minutes after the end of acupuncture. Ratings to pinprick and suprathreshold PPT were also recorded. The two groups were comparable in their demographic and baseline data. Analgesia induced by real or sham acupuncture did not differ on any outcome measures. PA responded to acupuncture needling better than PNA, and to sham needling (20% increase in PPT) better than to real acupuncture (7.9%). Those differences were at 20 min after end of acupuncture in the areas distant to the needling sites. PNA reported little changes in PPT. Being adaptive to pain was associated with enhanced distant analgesia in response to sham acupuncture. Our finding might partly explain varied acupuncture analgesia in clinical practice and trials.

Structural Isomers of a Potential Linking Ligand Containing a Pyridyl and a Carboxylate Terminals: (n-py)-CH=N-C₆H₄-CH₂-COOH} (n = 3, 4)

Zheng, Zhen Nu,Lee, Soon W. Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

Microwave-assisted Preparation, Structures, and Photoluminescent Properties of [Ln(NO₃)₂(H₂O)₃(L)₂](NO₃)(H₂O) {Ln=Tb, Eu;L=2-(4-pyridylium)ethanesulfonate, (4-pyH)⁺-C

Zheng, Zhen Nu,Lee, Soon-W. Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.6

Two lanthanide complexes, $[Ln(NO_3)_2(H_2O)_3(L)_2](NO_3)(H_2O)$ {Ln = Eu (1), Tb (2); L = 2-(4-pyridylium)-ethanesulfonate, $(4-pyH)^+-CH_2CH_2-SO_3^-)$}, were prepared from lanthanide nitrate and 4-pyridineethanesulfonic acid in $H_2O$ under microwave-heating conditions. Complexes 1 and 2 are isostructural, and the lanthanide metal in both complexes is coordinated to nine oxygen atoms. The pyridyl nitrogen in the ligand is protonated to give a zwitter ion that possesses an $NH^+$ (pyridyl) positive end and an $SO_3^-$ negative end. All O-H and N-H hydrogen atoms participate in hydrogen bonds to generate a two-dimensional (complex 1) or a three-dimensional network (complex 2). Complex 1 exhibits an intense red emission, whereas complex 2 exhibits an intense green emission in the solid state at room temperature.