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경사식 및 소형 터널식 인삼 해가림 시설에서 생육, 병해, 고온 피해 비교
배영석(Yeoung Seuk Bae),임은성(Eun Sung Lim),서수정(Su Jeoung Suh),유진(Jin Yu),장인배(In Bae Jang),장인복(In Bok Jang),김동휘(Dong Hwi Kim),김영창(Young Chang Kim) 한국약용작물학회 2021 한국약용작물학회지 Vol.29 No.6
Background: Ginseng cultivation is conducted in sun-shade facilities, and farmers are demanding new cultivation facilities with high durability and reusability. Methods and Results: In this study, three different sun-shade facilities, slope-shading type (SST), small connecting-tunnel (SCTT), and gradient-tunnel (GTT) were compared in terms of temperature and ginseng growth. The highest temperatures recorded, occuring at 13:00 on August 9, were 42.9℃ for the SCTT and 40.9℃ for the GTT. The SST was 38.5℃ at this time point, which was the lowest of the three treatment. When temperatures above 33℃ were cumulatively calculated from June to August, the SCTT was higher by 2,731.7℃ and 124.5℃ than the GTT and SST respectively. Plant length, stem length, leaf length, and leaf width were significantly higher in SCTT and GTT than in SST. The root weight was 14.7 g, 14.5 g, and 15.3 g in the SST, SCTT, and GTT, respectively, with the highest root weight in GTT. Anthracnose incidence was marginally higher in SST; 1.7% for the SST, 0.3% for the SCTT, and 0.2% for the GTT. Damage caused by high temperature was the highest in SST (8.0%), and was lower in SCTT (5.7%) and GTT (7.3%). Conclusions: We found that SCTT and GTT facilities are safe for ginseng cultivation. Future studies should investigate ways to lower the temperature, such as by installing light-shield film.
Young-Seuk Bae,배영석 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11
Cellular senescence is an irreversible form of cell cycle arrest. Senescent cells have a unique gene expression profile that is frequently accompanied by senescence-associated heterochromatic foci (SAHFs). Protein kinase CK2 (CK2) downregulation can induce trimethylation of histone H3 Lys 9 (H3K9me3) and SAHFs formation by activating SUV39h1. Here, we present evidence that the PI3K-AKTmTOR-reactive oxygen species-p53 pathway is necessary for CK2 downregulation-mediated H3K9me3 and SAHFs formation. CK2 downregulation promotes SUV39h1 stability by inhibiting its proteasomal degradation in a p53dependent manner. Moreover, the dephosphorylation status of Ser 392 on p53, a possible CK2 target site, enhances the nuclear import and subsequent stabilization of SUV39h1 by inhibiting the interactions between p53, MDM2, and SUV39h1. Furthermore, p21Cip1/WAF1 is required for CK2 downregulation-mediated H3K9me3, and dephosphorylation of Ser 392 on p53 is important for efficient transcription of p21Cip1/WAF1. Taken together, these results suggest that CK2 downregulation induces dephosphorylation of Ser 392 on p53, which subsequently increases the stability of SUV39h1 and the expression of p21Cip1/WAF1, leading to H3K9me3 and SAHFs formation.
Casein Kinase Ⅱ에 의한 송아지 흉선 DNA Topoisomerase Ⅱ 의 활성조절
이경희,배영석 ( Kyung Hee Lee,Young Seuk Bae ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.2
In order to study the modulation mechanism of topoisomerase II (topo II) activity, casein kinase II was purified from bovine liver using DEAF-cellulose, phosphocellulose, hydroxyapatite, and heparin-agarose column chromatography. Analysis of the purified enzyme by sodium dodesyl sulfate-polyacrylamide gel electrophoresis revealed three bands of molecular mass of 44, 42, and 26 kDa. Casein kinase II phosphorylated calf thymus topo II, and the phosphorylation stimulated topo II activity by 2 fold over that of unmodified enzyme. The treatment of calf thymus topo II with alkaline phosphatase abolished the enzyme activity almost completely. These results suggest that some of topo II enzymes exist as phosphoproteins in calf thymus and calf thymus topo II activity can be regulated by casein kinase II.
β 단백질의 중합을 억제하는 살균제에 대한 인삼 잿빛곰팡이병균의 감수성 변화
김주형,민지영,백영순,배영석,김흥태,Kim, Joo-Hyung,Min, Ji-Young,Baek, Young-Soon,Bae, Yeoung-Seuk,Kim, Heung-Tae 한국식물병리학회 2007 식물병연구 Vol.13 No.3
2005년과 2006년에 인삼 잿빛곰팡이병균을 각각 89개와 147개씩을 분리하여 실험에 사용하였다. Carbendazim은 $EC_{50}$값이 $10.0{\mu}g/ml$ 이상을, carbendazim/diethofencarb 합제는 $0.2{\mu}g/ml$ 이상을 저항성 검정의 기준으로 하였다. 이 기준을 가지고서 두 종류의 살균제에 대한 저항성 균주의 분리 빈도를 조사하면 carbendazim의 경우, 2005년에 87.6%, 2006년에는 96.6%로 나타나, 포장에서 분리되는 대부분의 Botrytis cinerea가 저항성균인 것으로 밝혀졌다. Carbendazim/diethofencarb 합제의 경우에는 저항성 균주의 분리빈도는 2005년과 2006년에 각각 23.6%와 24.5%로 나타났다. Carbendazim/diethofencarb 합제는 지역에 따라서 저항성균의 분리 빈도가 크게 달랐다. 경기도 연천 지역의 경우 저항성 균주의 분리빈도가 2005년에는 4.3%였고, 2006년에는 저항성 균주가 전혀 분리되지 않았다. 이에 비하여 다른 지역의 경우 저항성 균주의 빈도가 크게 높았는데, 2005년에는 경북 봉화와 예천 그리고 경기도 파주의 저항성 균주의 빈도가 각각 35.5%, 70.0% 및 40.0%였고, 2006년에는 전북 정읍의 저항성 균주의 빈도가 47.1%였다. 따라서 carbendazim/diethofencarb 합제에 대한 저항성 검정이 포장에서 지속적으로 수행되어야 한다. In this experiment, 236 isolates of Botrytis cinerea isolated from the lesions of ginseng grey mold in 2005 and 2006 were examined for their sensitivity to fungicide inhibiting ${\beta}-tubulin$ assembly. The baselines of fungicide resistance were determined as 10.0 and $0.2{\mu}g/ml$ of $EC_{50}$ values for carbendazim and the mixture of carbendazim and diethofencarb, respectively. The ratios of isolates resistant to carbendazim in 2005 and 2006 was investigated to be 87.6 and 96.6%, respectively. In the case of the mixture of carbendazim and diethofencarb, the ratio of the resistant isolates was 23.6% for 2005 and 24.5% for 2006. The ratio of the resistant isolates to the mixed fungicide was fluctuated according to regions where isolates of B. cinerea were obtained. In Yeoncheon of Gyeonggi Province, 4.3% of the isolates used in the experiment was resistant in 2005 and no resistant isolate was obtained in 2006. Among 5 regions, the ratio of resistant isolates was the highest as 70.0% in Yecheon of Gyeongbuk Province.
Diyah Fatimah Oktaviani(디야 파티마 옥타비아니),Young-Seuk Bae(배영석),Maria Dyah Nur Meinita(마리아 디아 누르 메이니타),Il Soo Moon(문일수),Yong-Ki Hong(홍용기) 한국생명과학회 2019 생명과학회지 Vol.29 No.10
수명이 짧은 예쁜꼬마선충은 수명 연장 등 많은 연구의 모델생물으로서 사용되고있다. 해조류 추출물들이 포함된 선충배양용 한천배지에서 선충(N2 야생형)을 키우면서 그 수명을 측정하였다. 13종의 흔한 해조류 중에서 갈조류 톳의 에탄올추출물이 난 부화, 성장 및 생존율에서 가장 큰 효과를 보였다. 그 수명은 에탄올추출물(0.05mg/ml) 및 주 활성성분인 fucosterol (0.05 mg/ml) 첨가에 의하여 1.54배 및 1.23배 정도로 유의미하게 증가되었다. 또한 에탄올추출물에 의하여 chemotaxis는 1.13배 증가, 한 배에서의 새끼는 0.74배 감소, 첫 산란시기는 0.96배 단축되었다. 이와 같은 결과들로 보아서 양식 가능한 해조류 톳은 건강에 이로운 건강보조 식품으로서의 좋은 재료가 될 수 있을 것이다. The short-lived nematode Caenorhabditis elegans has been used as a model organism for many studies, including lifespan extension. To screen common seaweeds for natural anti-aging agents, the lifespan of C. elegans (N2 wild-type strain) was measured by its hatch rate, growth rate, survival rate, chemotaxis, brood size, and egg-laying time after exposure to nematode growth medium (NGM) containing seaweed extracts. Approximately 30 animals synchronized at the first larval stage were incubated until they reached their adult stages before laying their eggs and were transferred to fresh NGM every 3 days. We also identified the major active compound from the seaweed by gas chromatography-mass spectrometry and tested its optimal dose for longevity. Of 13 common seaweed species, an ethanol extract of the brown seaweed Hizikia fusiformis showed the greatest effect on hatching, growth, and survival rates. The lifespan of C. elegans was significantly expanded 1.54-fold and 1.23-fold in the presence of the ethanol extract (0.05 mg/ml) and the main active component, fucosterol (0.05 mg/ml), respectively. Exposure to the ethanol extract also increased chemotaxis 1.13-fold, decreased brood size 0.74-fold, and shortened egg-laying time 0.96-fold. These results suggest that the aquaculturable H. fusiformis may be a promising source of a diet supplement to support health care.