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Yoon Young Choi,Sun Wook Han,Sang Ho Bae,Sung Yong Kim,Kyung Yul Hur,Gil Ho Kang 대한외과학회 2012 Annals of Surgical Treatment and Research(ASRT) Vol.82 No.1
Purpose: To compare the outcomes between laparoscopic total extraperitoneal (TEP) repair and prolene hernia system (PHS) repair for inguinal hernia. Methods: A retrospective analysis of 237 patients scheduled for laparoscopic TEP or PHS repair of groin hernia from 2005 to 2009 was performed. Results: The mean age was 52.3 years in TEP group and 55.7 years in PHS group. Of 119 TEP cases, 98 were indirect inguinal hernia, 15 direct type, 5 femoral hernia and 1 complex hernia; Of 118 PHS cases, 100 indirect, 18 direct type. All in TEP group were performed under general anesthesia and 64% of PHS group were performed under spinal or epidural anesthesia. Preoperatively, 10 cases of recurrent inguinal hernia were involved in our study (4 in TEP, 6 in PHS group). The mean operative time was similar in both groups (74.8 in TEP, 71.2 in PHS group), however mean hospital stay (1.6 days in TEP, 3.2 days in PHS group, P = 0.018) and mean usage of analgesics (0.54 times in TEP, 2.03 times in PHS group, P < 0.01), complications (36 cases in TEP, 6 cases in PHS group, P < 0.01) showed statistical differences. There is only 1 case of postoperative recurrence inguinal hernia in PHS group but it has no statistical significance (P = 0.314). Conclusion: Compared to PHS repair, laparoscopic TEP repair has some advantages; shorter hospital stay, less frequent need of analgesics; as well as more postoperative complications such as hematoma, seroma, scrotal swelling.
Meiotic prophase roles of Rec8 in crossover recombination and chromosome structure
Yoon, Sang-Wook,Lee, Min-Su,Xaver, Martin,Zhang, Liangran,Hong, Soo-Gil,Kong, Yoon-Ju,Cho, Hong-Rae,Kleckner, Nancy,Kim, Keun P. Oxford University Press 2016 Nucleic acids research Vol.44 No.19
<P>Rec8 is a prominent component of the meiotic prophase chromosome axis that mediates sister chromatid cohesion, homologous recombination and chromosome synapsis. Here, we explore the prophase roles of Rec8. (i) During the meiotic divisions, Rec8 phosphorylation mediates its separase-mediated cleavage. We show here that such cleavage plays no detectable role for chromosomal events of prophase. (ii) We have analyzed in detail three <I>rec8</I> phospho-mutants, with 6, 24 or 29 alanine substitutions. A distinct ‘separation of function’ phenotype is revealed. In the mutants, axis formation and recombination initiation are normal, as is non-crossover recombination; in contrast, crossover (CO)-related events are defective. Moreover, the severities of these defects increase coordinately with the number of substitution mutations, consistent with the possibility that global phosphorylation of Rec8 is important for these effects. (iii) We have analyzed the roles of three kinases that phosphorylate Rec8 during prophase. Timed inhibition of Dbf4-dependent Cdc7 kinase confers defects concordant with <I>rec8</I> phospho-mutant phenotypes. Inhibition of Hrr25 or Cdc5/polo-like kinase does not. Our results suggest that Rec8's prophase function, independently of cohesin cleavage, contributes to CO-specific events in conjunction with the maintenance of homolog bias at the leptotene/zygotene transition of meiotic prophase.</P>
Yoon, Cheol-Hee,Jang, Dai-Ho,Kim, Kyung-Chang,Park, Soon Young,Kim, Hye-young,Kim, Sung Soon,Chi, Sung-Gil,Choi, Byeong-Sun S.Karger 2014 Intervirology Vol.57 No.2
<P>Abstract</P><P><B><I>Objectives:</I></B> Persistent HIV-1 infections are characterized by a long silent infection period in resting CD4+ T cells, which allows them to escape the host immune response. Several HIV-1 latency mechanisms have been reported, but the molecular mechanism underlying polycomb repressor complex (PRC)-mediated HIV-1 latency remains poorly understood. <B><I>Methods:</I></B> Expression of PRC proteins in latent cells was measured by Western blot. Knockdowns of PRC genes were conducted by the specific siRNA and methylations at H3K27 on the proviral LTR were investigated by ChIP assay. <B><I>Results:</I></B> PRC proteins (EED, BMI-1, and RNF2) were dramatically downregulated in latent cells after PMA treatment. The downregulation of PRC proteins was followed by a decrease in the methylation of H3K27 and ubiquitination of H2AK119 in the PMA-treated latent cells. siRNA knockdowns of EED and BMI-1 also enhanced HIV-1 reactivation significantly in latently infected cells. By contrast, proteasomal inhibitor MG132 successfully abrogated the PMA-induced downregulation of PRCs. In particular, di-/tri-methylations of histone-3 in the proviral LTR was absent from latent cells after PMA treatment. <B><I>Conclusions:</I></B> This study shows that PRC is strongly related to the control of HIV-1 latency and that PRC-breaking agents may be helpful for purging HIV-1 from latent reservoirs.</P><P>© 2014 S. Karger AG, Basel</P>
Development of 3D-based On-Machine Measurement Operating System
Yoon Gil-Sang,Heo Young-Moo,Kim Gun-Hee,Cho Myeong-Woo Korean Society for Precision Engineering 2005 International Journal of Precision Engineering and Vol.6 No.3
This paper proposed an efficient manufacturing system using the OMM (on-machine measurement) system. The OMM system is software-based 3D modeler for inspection on machine, and it is interfaced with machine tools via RS232C. The software is composed of two inspection modules; one is touch probe operating module, and the other is laser displacement sensor operating module. The module for touch probe needs the inspection feature extracted from CAD data. The touch probe moves to workpiece by three operating modes as follows: manual, general and automatic mode. The operating module of the laser displacement sensor is used to inspect profiles and very small holes. An advantage of this inspection method is the ability to execute on-line inspection during machining or afterward. The efficiency of proposed system which can predict and define the machining errors of each process was verified, so the developed system was applied to inspect a mold-base (cavity, core).
Sang-Rae Moon,Sun-Ran Cho,Jin-Won Jeong,Youn-Ho Shin,Jeong-Oh Yang,Ki-Su Ahn,Changmann Yoon,Gil-Hah Kim 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Attraction responses of plant essential oils were investigated, and the electrophysiological response to nymphs and adults of spot clothing wax cicada (Lycorma delicatula) was confirmed. Of the ten tested oils, only spearmint oil was found attractive. In dose responses of spearmint oil, second to fourth instar nymphs, as well as adults, were significantly attracted to a dose of 5 μL; for nymphs, fourth instar nymph showed greatest attraction response (90.9%), and second and third instar nymphs showed mild attraction. At a dose of 10 μL, fourth instar nymphs and adults were significantly attracted to spearmint oil. Only fourth instar nymphs were attracted to spearmint oil at 2.5 μL. After analyzing spearmint oil using gas chromatography (GC) and GC/mass spectrometry, carvone constituent was found as a significant attractant for both nymphs and adults, except for first instar nymphs. Limonene did not show any attraction response. All constituents mixed with each other appeared to have an additive effect. In electrophysiological response to spearmint oil, antennae of only fourth instar nymphs and female adults responded to carvone. Therefore, spearmint oil may be effective as an attractant for control of L. delicatula populations. In a field test, fourth nymphs and female adults were highly attracted to 20 μL of spearmint oil. This is the first report on attraction response of L. delicatula to spearmint oil in the laboratory and the field.
Sang-Hyun Kang,Shin Hwang,Tae Yong Ha,Gi Won Song,Dong-Hwan Jung,Chul-Soo Ahn,Deok-Bog Moon,Ki-Hun Kim,Gil-Chun Park,Young-In Yoon,Yo Han Park,Hui-Dong Cho,권재현,Yong-Kyu Chung,Jin-Uk Choi,Sung Gyu Lee 대한이식학회 2019 Korean Journal of Transplantation Vol.33 No.4
Background: The mammalian target of the rapamycin inhibitor has dual inhibitory effects on cell growth and angiogenesis. This study aimed to analyze the usage of everolimus on actual immunosuppression (IS) regimens through a cross-sectional study in a high-volume liver transplantation (LT) center. Methods: Our institutional LT database was searched for adult patients who underwent primary LT surgery between January 2010 and December 2016. We identified 2,093 LT recipients with observation periods of 1 to 8 years. Results: We divided the 2,093 recipients into three groups according to the posttransplant follow-up period as follows: group A (12–36 months; n=680), group B (37–60 months; n=560), and group C (>60 months; n=853). The individual IS agents were tacrolimus in 1,807 patients (86.3%), cyclosporine in 169 patients (8.1%), mycophenolate mofetil (MMF) in 1,310 patients (62.6%), and everolimus in 115 patients (5.5%). The most common IS regimens were tacrolimus-MMF combination and tacrolimus monotherapy, regardless of the posttransplant period. Patients with pretransplant malignancies were administered everolimus more frequently than those without pretransplant malignancies (P<0.001). In 102 patients with hepatocellular carcinoma recurrence or de novo malignancies, IS regimens included everolimus-tacrolimus in 41 patients (40.2%), tacrolimus-MMF in 27 patients (26.4%), tacrolimus in 20 patients (19.6%), MMF in 10 patients (9.8%), cyclosporine in three patients (2.9%), and cyclosporine-MMF in one patient (1.0%). Conclusions: Administration of everolimus after LT has been gradually increasing with the expansion of indications in our institutional practice. Currently, the role of everolimus is minimal and not comparable to that of tacrolimus, but it has a unique position in the field of IS after LT.
Yoon Ah Kim,Gil Jae Jung,Hae Ryung Choi,김종수,이상준,Phil Sang Chung,우승훈 대한의학레이저학회 2022 MEDICAL LASERS Vol.11 No.1
Background and ObjectivesPulsed Nd:YAG lasers have been used in various medical fields and show high second harmonic generation efficiency at 532 nm. The pulse duration at 532 nm varies from 10 ns to 1000 ns, depending on the excitation method. This study aimed to compare the effects of 100 ns and 600 ns, 532 nm flashlamp excited solid (FPSS) and diode excited solid (DPSS) lasers on guinea pig muscles and skin.Materials and MethodsExperiments were carried out on the guinea pig muscles and skin. First, the guinea pig muscles, and skin were irradiated with 532 nm FPSS and DPSS lasers at varying intensities and periods. A visual examination of the muscle lesions and an immediate histopathological examination of the skin were performed.ResultsWhen guinea pig muscles were irradiated with FPSS and DPSS lasers, the degree of light evaporation and photo necrosis was much higher with the FPSS laser. There was also higher guinea pig muscle and skin vaporization and necrosis with the FPSS laser than with the DPSS laser when both lasers were used to irradiate the guinea pig skin at power levels of 1 watt (63.7 J/cm2), 2 watts (127.3 J/cm2), 4 watts (254.6 J/cm2), and 8 watts (509.3 J/cm2).ConclusionThe DPSS laser system appears to be more effective and even safer than the FPSS laser system; it delivers more satisfactory surgical results and causes less serious damage to the surrounding tissue.