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Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12
Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.
Hydrogels with diffusion-facilitated porous network for improved adsorption performance
Yan-yan Pei,Dong-mei Guo,Qing-da An,Zuo-yi Xiao,Shangru Zhai,Bin Zhai 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.12
Porous alginate-based hydrogel beads (porous ABH) have been prepared through a facile and sustainable template-assisted method using nano-calcium carbonate and nano-CaCO3 as pore-directing agent for the efficient capture of methylene blue (MB). The materials were characterized by various techniques. The sorption capacities of ABH towards MB were compared with pure sodium alginate (ABH-1:0) in batch and fixed-bed column adsorption studies. The obtained adsorbent (ABH-1:3) has a higher BET surface area and a smaller average pore diameter. The maximum adsorption capacity of ABH-1:3 obtained from Langmuir model was as high as 1,426.0mg g1. The kinetics strictly followed pseudo-second order rate equation and the adsorption reaction was effectively facilitated, approximately 50minutes to achieve adsorption equilibrium, which was significantly shorter than that of ABH-1:0. The thermodynamic parameters revealed that the adsorption was spontaneous and exothermic. Thomas model fitted well with the breakthrough curves and could describe the dynamic behavior of the column. More significantly, the uptake capacity of ABH-1:3 was still higher than 75% of the maximum adsorption capacity even after ten cycles, indicating that this novel adsorbent can be a promising adsorptive material for removal of MB from aqueous solution under batch and continuous systems.
Eccentric strength and design of RC columns strengthened with SCC filled steel tubes
Yi-Yan Lu,Hong-Jun Liang,Shan Li,Na Li 국제구조공학회 2015 Steel and Composite Structures, An International J Vol.18 No.4
Self-compacting Concrete Filled steel Tubes (SCFT), which combines the advantages of steel and concrete materials, can be applied to strengthen the RC columns. In order to investigate the eccentric loading behavior of the strengthened columns, this paper presents an experimental and numerical investigation on them. The experimental results showed that the use of SCFT is interesting since the ductility and the bearing capacity of the RC columns are greatly improved. And the performance of strengthened columns is significantly affected by four parameters: column section type (circular and square), wall thickness of the steel tube, designed strength grade of strengthening concrete and initial eccentricity. In the numerical program, a generic fiber element model which takes in account the effect of confinement is developed to predict the behavior of the strengthened columns subjected to eccentric loading. After the fiber element analysis was verified against experimental results, a simple design formula based on the model is proposed to calculate the ultimate eccentric strength. Calibration of the calculated results against the test results shows that the design formula closely estimates the ultimate capacities of the eccentrically compressed strengthened columns by 5%.
Yan-Li Yi,Jin-Bao Wu 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.5
An organic-inorganic hybrid sorbent with high adsorption capacity was prepared by surface imprinting technique combined with sol-gel processing with 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane as a functional precursor and silica as the support for the removal of Cd(II) ion from aqueous solution, and was characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, nitrogen gas sorption and thermogravimetric analysis. The influences of different adsorption parameters, such as pH value of solution, contact time and the initial concentrations of Cd(II) ions on the adsorption amount of Cd(II), were examined. The optimum pH for adsorption was found to be in the range of 4-8. The adsorption rate of Cd(II) on the imprinted hybrid sorbent was rapid. The relative selectivity coefficients of the imprinted hybrid sorbent were higher than those of the non-imprinted sorbent. Ho’s pseudosecond-order model best described the kinetics of the adsorption reaction. The adsorption process of metals followed Redlich-Peterson and Langmuir isotherms models, and the experimental value of maximum adsorption capacity for Cd(II) was 77.2mg·g−1. The positive values of ΔHo suggested endothermic nature of Cd(II) adsorption on the imprinted hybrid sorbent. Increase in entropy of adsorption reaction was shown by the positive values of ΔSo and the negative values of ΔGo indicating that the adsorption was spontaneous.
Yi Li,Yuanjie Ni,Wei Liu,Wenxing Yan 보안공학연구지원센터(IJUNESST) 2015 International Journal of u- and e- Service, Scienc Vol.8 No.1
In the network innovation environment, the knowledge trading between enterprises could be divided into traditional pattern (TP) and the E-commerce pattern (ECP). The two both have their advantages and disadvantages, so enterprises would face the problem. This paper generalizes the operation characteristics and the influence factors of two patterns. The mathematical model is used to analyze the selection mechanism of choosing two patterns, and the result illustrated that when the agency fee, the risk cost of ECP and the trading potential get bigger, the possibility that enterprises choose TP would get bigger, too; and when the explicitness of knowledge, cost of searching, trading incentive, the risk cost of TP and the reserve cost of ECP increase, the possibility that enterprises choose ECP would increase. When the reserve cost of ECP becomes big enough, it would make enterprises be more willing to choose hybrid pattern.
Yan, Yi-Dong,Kim, Jung Ae,Kwak, Mi Kyung,Yoo, Bong Kyu,Yong, Chul Soon,Choi, Han-Gon Pharmaceutical Society of Japan 2011 Biological & pharmaceutical bulletin Vol.34 No.8
<P>In this study, a novel liquid self-emulsifying drug delivery system (SEDDS) containing curcumin was formulated and further developed into a solid form by a spray drying method using Aerosil 200 as the solid carrier. The optimum liquid SEDDS consisted of Lauroglycol Fcc, Labrasol and Transcutol HP as the oil phase, the surfactant and the co-surfactant at a weight ratio of 15.0 : 70.8 : 14.2 (w/w/w), respectively. There was no difference in droplet size between the emulsions obtained from the liquid and solid forms of SEDDS. Solid state characterization of the solid SEDDS was performed by scanning electron micrograph (SEM), differential scanning calorimetry (DSC), and X-ray powder diffraction (XRPD). The drug formulated in the solid SEDDS was quickly and completely dissolved within 5 min, both in 0.1 <SMALL>N</SMALL> HCl and phosphate buffer pH 6.8 dissolution media, whereas crude curcumin powder was significantly less dissoluble. The solid SEDDS formulation was stable for at least 3 months at 40°C with 75% relative humidity. After oral administration to rats, curcumin in the solid SEDDS resulted in significant improvement in <I>in vivo</I> absorption compared with that of curcumin powder. As the dose of curcumin formulated in solid SEDDS increased from 25 to 100 mg/kg, the <I>C</I><SUB>max</SUB> and area under the drug concentration time curve (<I>AUC</I>) of curcumin were increased by 4.6 and 7.6 times, respectively. However, the over-proportional increase in the <I>AUC</I> in the higher dose group might be due to underestimation of <I>AUC</I> in the lower dose group. In conclusion, this solid SEDDS is a promising solid dosage form for poorly water-soluble curcumin.</P>