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Controlled drug release using ascorbate-responsive quercetin-conjugated alginate hydrogels
Nam, Jungchan,Yeo, Woon-Seok The Korean Society for Applied Biological Chemistr 2016 Applied Biological Chemistry (Appl Biol Chem) Vol.59 No.4
In this paper, we report a new strategy for controlled cargo release using quercetin-conjugated alginate (Alg) hydrogels that has enhanced hydrogel stability and can release cargo molecules through on-demand metal cation reduction by a biological reducing agent, ascorbate. By conjugating hydrophobic quercetin to the Alg backbone, hydrogel stability was increased. The encapsulated cargos were released on demand through reduction of cross-linking Fe(III) ions by ascorbate, harnessing the differences in cross-linking capability between Fe(II) and Fe(III). We found that encapsulated fluorescein in quercetin-conjugated Alg hydrogels was released much slower than that in hydrogels without quercetin conjugation. In addition, the stability of the hydrogels and the release of encapsulated fluorescein were controlled by modulating oxidation state of Fe using ascorbate. We validated our strategy by demonstrating the enhanced cancer cell killing of the doxorubicin-encapsulated hydrogels upon treatment with ascorbate. We believe that the strategy would be used as an effective tool for the anticancer drug delivery that can be controlled by ascorbate as overcoming the drawback of Alg hydrogels.
RF/DC 마그네트론 스퍼터로 제조한 NiInZnO/Ag/NiInZnO 다층박막의 Ag 금속 삽입층 두께 변화에 따른 특성 연구
김남호(Nam-Ho Kim),김은미(Eun-Mi Kim),허기석(Gi-Seok Heo),여인선(In-Seon Yeo) 대한전기학회 2016 전기학회논문지 Vol.65 No.12
Transparent, conductive electrode films, showing the particular characteristics of good conductivity and high transparency, are of considerable research interest because of their potential for use in opto-electronic applications, such as smart window, photovoltaic cells and flat panel displays. Multilayer transparent electrodes, having a much lower electrical resistance than widely-used transparent conducting oxide electrodes, were prepared by using RF/DC magnetron sputtering system. The multilayer structure consisted of three layers, [NiInZnO(NIZO)/Ag/NIZO]. The optical and electrical properties of the multilayered NIZO/Ag/NIZO structure were investigated in relation to the thickness of each layer. The optical and electrical characteristics of multilayer structures have been investigated as a function of the Ag and NIZO film thickness. High-quality transparent conductive films have been obtained, with sheet resistance of 9.8 Ω/sq for Ag film thickness of 8 ㎚. Also the multilayer films of inserted Ag 8 nm thickness showed a high optical transmittance above 93% in the visible range. The electrical and optical properties of the new multilayer films were mainly dependent on the thickness of Ag insertion layer.
Ko, Seok-Jae,Park, Jae-Woo,Lee, Jae-hyung,Lee, Jung-eun,Ha, Na-yeon,Nam, Seong-uk,Lee, Jae-hong,Jeon, Soo-Hyung,Kim, Jong-Won,Kang, Changwan,Yeo, Inkwon,Kim, Jinsung Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-
<P><B>Introduction</B></P><P> Functional dyspepsia (FD) is a functional gastrointestinal disorder characterized by persistent upper dyspeptic symptoms without organic lesions. There is no standard therapy for FD. Yukgunja-tang (YGJT) is an herbal medicine used for treating upper gastrointestinal symptoms in Asia. Studies on the effect of YGJT on FD have been conducted. However, the results were inconsistent. In<I> Hyungsang</I> medicine, traditional Korean medicine, FD patients are classified into bladder body (BB) or gallbladder body (GB) subtypes by the shape and angle of their faces. Each subtype may have different characteristics, physiology, and pathology of the same disease. YGJT is more effective for patients with BB subtype. The three-dimensional facial shape diagnostic system (3-FSDS) was shown to be effective in diagnosing BB or GB subtypes. This study aimed to investigate the effect of YGJT on FD patients classified using the 3-FSDS.</P><P><B> Materials and Methods</B></P><P> The current study was a placebo-controlled, double-blinded, randomized, two-center trial. Eligible patients were diagnosed with either BB or GB FD subtype using the 3-FSDS. Ninety-six participants (48 BB and 48 GB subtypes) were randomly allocated to treatment or control groups in a 2:1 ratio. YGJT or placebo was administered for eight weeks. The primary outcome was assessed using the total dyspepsia symptom scale (TDS), while the secondary outcomes were assessed using the single dyspepsia symptom scale (SDS), proportion of responders, visual analog scale, Nepean dyspepsia index, functional dyspepsia-related quality of life, and spleen qi deficiency questionnaire.</P><P><B> Results and Discussion</B></P><P> The result of TDS showed the superior effect of YGJT on BB over GB subtype. The subgroup analysis of TDS and SDS scores showed the superior effect of YGJT over placebo. Other outcome variables did not show any significant differences between groups.</P><P><B> Conclusion</B></P><P> YGJT may be considered for FD patients diagnosed with BB subtype using 3-FSDS.</P>
( Joon Seok ),( In Kwon Yeo ),( Kui Young Park ),( Seong Jun Seo ),( Myeung Nam Kim ),( Sung Eun Chang ),( Chong Hyun Won ),( Yang Won Lee ) 대한피부과학회 2014 대한피부과학회 학술발표대회집 Vol.66 No.2
Background: Hyaluronic acid(HA) fillers have become popular soft tissue filler augmentation agents over the past several years. Many new brands of HA fillers are being produced, but comparative research on the characteristics of similar products is limited. Objectives: The purpose of this study was to test the efficacy, tolerability, and safety of a new HA filler, PP-501-B (Cleviel contour®, Pacific pharm., Korea) which is used for correcting nasolabial folds (NLFs), and compare PP-501-B with Restylane Perlane® (Q-Med, Uppsala, Sweden). Methods: One hundred three subjects with visible NLFs were enrolled in a randomized, multi-center, patient & evaluator-blind, active-controlled, matched-pair clinical study. Each subject was injected with PP-501-B in one NLF and Restylane Perlane® in the other. All participants were then reassessed for cosmetic change at 8, 16, and 24 weeks. Wrinkle severity was rated using the 5-point wrinkle severity rating scale (WSRS) Results: At week 24, the mean improvement in the WSRS from baseline was 1.87 ± 0.73 for the PP-501-B side and 1.92 ± 0.71 for the Restylane Perlane®. Both fillers were well-tolerated and adverse reactions were mild and transient in most cases. Conclusion: The addition of a new HA filler, PP-501-B with suitable characteristics and ample safety profiles to the market will widen the selection for physicians and patients because the purpose, area, and depth of filler injections are variable by treatment.
이여주 ( Yeo Joo Lee ),초가기 ( Jia Qi Chu ),이서용 ( Seo Yong Lee ),김수미 ( Su Mi Kim ),이광녕 ( Kwang Nyeong Lee ),고영준 ( Young Joon Ko ),이향심 ( Hyang Sim Lee ),조인수 ( In Soo Cho ),남석현 ( Seok Hyun Nam ),박종현 ( Jong H 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.2
Asia1/Shamir that has been recommended by World Reference Laboratory for foot-and-mouth disease (FMD) is used as a vaccine strain, and is being prepared in many countries including Korea. Although it is assumed that vaccine strain Asia1/Shamir has a wide antigenicity, sufficient molecular biological analysis has not been accomplished yet. Complete genome sequence analysis showed that the region with the most severe variations was 1D region of structural protein-coding sequence; particularly amino acid 141~157 residues in 1D region RGD sites for binding to susceptible cells. In addition, five amino acids in 1D region were identified as characteristic sites that are different from other known Asia1 viruses. Asia1/Shamir strain was shown to be genetically similar to group VI that had occurred in the Middle East, but showed low level of genetic similarity to the group V viruses that had occurred in the Southeast Asia and China. It is considered that, if these viruses, group I and II including group V are introduced into Korea, care would be paid in case of inoculating the vaccine strain Shamir available in Korea.
Park, Yeo-Ul,Jung, Jong-Hyun,Seo, Dong-Ho,Jung, Dong-Hyun,Kim, Jae-Han,Seo, Ean-Jeong,Baek, Nam-In,Park, Cheon-Seok Elsevier 2018 Enzyme and microbial technology Vol.114 No.-
<P><B>Abstract</B></P> <P> <I>Desulfurococcus amylolyticus</I> is an anaerobic and hyperthermophilic crenarchaeon that can use various carbohydrates as energy sources. We found a gene encoding a glycoside hydrolase family 57 amylolytic enzymes (DApu) in a putative carbohydrate utilization gene cluster in the genome of <I>D. amylolyticus</I>. This gene has an open reading frame of 1,878 bp and consists of 626 amino acids with a molecular mass of 71 kDa. Recombinant DApu (rDApu) completely hydrolyzed pullulan to maltotriose by attacking α-1,6-glycosidic linkages, and was able to produce glucose and maltose from soluble starch and amylopectin. Although rDApu showed no activity toward α-cyclodextrin (CD) and β-CD, maltooctaose (G8) was detected from reaction with γ-CD. The highest activity of rDApu was measured at pH 5.0 and 95 °C. The half-life of rDApu was 12.7 h at 95 °C and 27 min at 98 °C. Interestingly, rDApu was able to transfer a maltose unit to 6-<I>O</I>-α-maltosyl-β-CD via transglycosylation. Structure analysis using MALDI-TOF/TOF MS and nuclear magnetic resonance revealed that the new transglycosylated products were 6<SUP>1</SUP>, 6<SUP>4</SUP>-di-<I>O</I>-maltosyl-β-CD and 6<SUP>1</SUP>, 6<SUP>3</SUP>, 6<SUP>5</SUP>-tri-<I>O</I>-maltosyl-β-CD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> GH57 amylopullulanase from <I>Desulfurococcus amylolyticus</I> (DApu) was characterized. </LI> <LI> DApu exhibits not only hydrolysis activity but also transglycosylation activity. </LI> <LI> Branched cyclodextrins were formed by transfer of a maltose unit by DApu. </LI> </UL> </P>