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- 저자 : Yanhong Dai (검색결과 3 건)
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Wenshuai Zhu,Huaming Li,Yehai Xu,Bilian Dai,Hui Xu,Chao Wang,Yanhong Chao,Hui Liu 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.2
Three types of TiO2 were synthesized by a hydrolysis and calcination method. The catalysts were characterizedby X-ray powder diffraction (XRD), diffuse reflectance spectrum (DRS), Raman spectra, and X-ray photoelectronspectroscopy (XPS). The XRD and Raman spectra indicated that amorphous TiO2 was successfully obtained at100 oC. The results indicated that amorphous TiO2 achieved the highest efficiency of desulfurization. The photocatalyticoxidation of dibenzothiophene (DBT), benzothiophene (BT), 4,6-dimethyldibenzothiophene (4,6-DMDBT) anddodecanethiol (RSH) in model oil was studied at room temperature (30 oC) with three catalysts. The system containedamorphous TiO2, H2O2, and [Bmim]BF4 ionic liquid, ultraviolet (UV), which played vitally important roles in the photocatalyticoxidative desulfurization. Especially, the molar ratio of H2O2 and sulfur (O/S) was only 2 : 1, which correspondedto the stoichiometric reaction. The sulfur removal of DBT-containing model oil with amorphous TiO2 couldreach 96.6%, which was apparently superior to a system with anatase TiO2 (23.6%) or with anatase - rutile TiO2 (18.2%). The system could be recycled seven times without a signicant decrease in photocatalytic activity.
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Xiuling Zhang,Jinxiang Chen,Ziwen Gao,Hui Qi,Yanhong Dai,Wandong She 대한이비인후과학회 2019 Clinical and Experimental Otorhinolaryngology Vol.12 No.4
Objectives. To investigate glucocorticoid receptor (GR) and histone deacetylase 2 (HDAC2) gene expression and protein levels in peripheral blood mononuclear cells (PBMCs) of patients with severe or profound sudden sensorineural hearing loss (SSNHL) and to explore the roles of GRs and HDAC2 in glucocorticoid (GC) insensitivity. Methods. Fifty-five severe or profound SSNHL patients were enrolled in the study. According to hearing improvement after GC treatment, patients were assigned into two groups: GC-sensitive and GC-resistant. A normal reference group included 20 healthy volunteers without hearing loss. Quantitative real-time polymerase chain reaction and Western blot analyses were used to detect the relative expression of GRα, GRβ, and HDAC2 in PBMCs at the mRNA and protein levels. Results. The protein levels of GRs and HDAC2 in PBMCs of SSNHL patients were lower than the normal reference values before GC treatment. Compared with the GC-resistant group, both the mRNA and protein levels of GRα and HDAC2 were significantly increased in the GC-sensitive group after GC treatment. Conclusion. A lack of GRα and HDAC2 induction following steroid treatment in GC-resistant SSNHL patients may play a fundamental mechanistic role in GC insensitivity. Response of GRα and HDAC2 to steroid treatment may, thus, predict the prognosis of hearing improvement in SSNHL patients.
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Culture of a Whole Porcine Liver Ex Situ without Red Blood Cells
( Jing Dong ),( Lingling Xia ),( Hefang Shen ),( Congwen Bian ),( Sujin Bao ),( Ming Zhang ),( Yan Dai ),( Yanhong Xu ),( Qiru Xiong ),( Jianjian Xu ),( Lili Xu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1
Aims: Liver transplantation is an effective approach to end-stage liver disease. Shortage of donor liver and increased waiting time for liver transplantation necessitate the development of an organ culture system by which livers can be cultured and maintained ex situ for a prolonged period of time. The aim of this work is to test whether cell culture condition in vitro could be used to culture whole livers ex situ without the use of erythrocytes. Methods: Eight castrated male land race/farm young porcine livers were exposed to 30 min warm ischemia and 30 min cold perfusion. Livers were isolated and connected to an ex situ liver culture system using a standard culture medium RPMI 1640 supplied with 10% of fetal calf serum and sufficient dissolved oxygen under a normothermic condition for 6 hours. Metabolic biomarkers, bile and urea production, hepatic cell viability, and histology analysis of biopsies were performed and analyzed. Results: Dissociated porcine hepatic cells survived and grew in vitro under the standard RPMI 1640 culture medium. When the same RPMI 1640 medium supplemented with 10% of FCS and sufficient oxygen was used to culture livers ex situ, over 98% of liver cells were viable for at least 6 hours during ex situ whole organ culture based on the results from biochemical assays. Conclusions: Our data demonstrate that the liver culture sys tem established in this work can be used to culture whole livers ex situ in the absence of erythrocytes.
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