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        Fermentation characteristics and microbial community composition of wet brewer's grains and corn stover mixed silage prepared with cellulase and lactic acid bacteria supplementation

        Guoqiang Zhao,Hao Wu,Yangyuan Li,Li Li,Jiajun He,Xinjian Yang,Xiangxue Xie Asian Australasian Association of Animal Productio 2024 Animal Bioscience Vol.37 No.1

        Objective: The objective of this study was to investigate how cellulase or/and lactic acid bacteria (LAB) affected the fermentation characteristic and microbial community in wet brewer's grains (WBG) and corn stover (CS) mixed silage. Methods: The WBG was mixed thoroughly with the CS at 7:3 (w/w). Four treatment groups were studied: i) CON, no additives; ii) CEL, added cellulase (120 U/g fresh matter [FM]), iii) LAB, added LAB (2×10<sup>6</sup> cfu/g FM), and iv) CLA, added cellulase (120 U/g FM) and LAB (2×10<sup>6</sup> cfu/g FM). Results: All additive-treated groups showed higher fermentation quality over the 30 d ensiling period. As these groups exhibited higher (p<0.05) LAB counts and lactic acid (LA) content, along with lower pH value and ammonia-nitrogen (NH<sub>3</sub>-N) content than the control. Specifically, cellulase-treated groups (CEL and CLA) showed lower (p<0.05) neutral detergent fiber and acid detergent fiber contents than other groups. All additives increased the abundance of beneficial bacteria (Firmicutes, Lactiplantibacillus, and Limosilactobacillus) while they decreased abundance of Proteobacteria and microbial diversity as well. Conclusion: The combined application of cellulase and LAB could effectively improve the fermentation quality and microbial community of the WBG and CS mixed silage.

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        Mitogenome evidence for the existence of cryptic species in Coelomactra antiquata

        Yang Yuan,Lingfeng Kong,Qi Li 한국유전학회 2013 Genes & Genomics Vol.35 No.6

        Cryptic species which improve our understanding of species diversity and evolutionary histories within marine animals have been increasingly revealed in the marine realm. Coelomactra antiquate is an important commercial bivalve species, but has been suffering from severe population decline due to over-exploitation and deterioration of environmental conditions. To test the hypothesis that cryptic species might exist in C. antiquate presented in previous study, four complete mitogenomes of C. antiquate from northern and southern China were determined here. Comprehensive comparative analysis of the mitochondrial genomes of C. antiquate between northern and southern population reveals significant differences in genome composition,protein coding genes, tRNA genes, non-coding regions, genetic divergence and phylogenetic analysis. The results provide strong mitogenome evidence for the existence of cryptic species in C. antiquate. Besides, our results also support that comprehensive comparative analysis of mtDNA represents an accessible and powerful tool to identify cryptic species.

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        Isolation and characterization of polymorphic microsatellite loci in the grand jackknife clam Solen grandis (Bivalvia: Veneroida)

        Yang Yuan,Lingfeng Kong,Xiaodong Zheng,Qi Li 한국유전학회 2010 Genes & Genomics Vol.32 No.3

        The grand jackknife clam Solen grandis (Dunker, 1861) is an overexploited bivalve species. In order to promote a conservation program for this species, it is necessary to evaluate its genetic diversity and population genetics. For this purpose,we isolated the first set of 18 polymorphic microsatellite markers and examined their allelic variation using a total of 30wild individuals collected from one locality. The number of alleles per locus ranged from 5 to 24, and the observed and expected heterozygosity varied from 0.300 to 0.967 and from 0.721 to 0.952, respectively. The high variabilities at these loci indicated that they would be use

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