http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Xiaoxu Yang,Yinshan Guo,Junchi Zhu,Guangli Shi,Zaozhu Niu,Zhendong Liu,Kun Li,Xiuwu Guo 한국유전학회 2017 Genes & Genomics Vol.39 No.10
Linalool, α-terpineol, citronellol, nerol, geraniol, and geraniol acid are the main monoterpenes in the grapevine (Vitis vinifera L.) with the concentrations of these substances differing in different varieties. This research performed association analyses using a core collection of 92 grape accessions to identify causal SNPs in the gene 1-deoxy-d-xylulose-5-phosphate synthase (VvDXS), a regulator of monoterpene metabolism. We evaluated the free linalool, α-terpineol, citronellol, nerol, geraniol, and geraniol acid content of 92 grape varieties in two consecutive years (2014 and 2015). Twenty-two SNPs were found in the VvDXS coding region and were used in an association analysis. We found that the linalool, α-terpineol, nerol, and geraniol acid levels were higher in varieties with a G/T genotype at P852 than in varieties with other genotypes at this site. Additionally, we found a novel polymorphism site P1678 (A/G) with a functional effect on terpenoid content that was related to the regulation of gene transcription. This study revealed the relationship between VvDXS and aroma, with the identified polymorphism site potentially assisting in the screening of aroma compounds at early stages in fruit development in grapevines.
HCBP6 upregulates human SREBP1c expression by binding to C/EBPβ-binding site in the SREBP1c promoter
( Xueliang Yang ),( Ming Han ),( Shunai Liu ),( Xiaoxue Yuan ),( Xiaojing Liu ),( Shenghu Feng ),( Li Zhou ),( Yaru Li ),( Hongping Lu ),( Jun Cheng ),( Shumei Lin ) 생화학분자생물학회(구 한국생화학분자생물학회) 2018 BMB Reports Vol.51 No.1
Sterol regulatory element-binding protein-1c (SREBP1c) plays an important role in triglyceride (TG) homeostasis. Although our previous study showed that hepatitis C virus core-binding protein 6 (HCBP6) regulates SREBP1c expression to maintain intracellular TG homeostasis, the mechanism underlying this regulation is unclear. In the present study, we found that HCBP6 increased intracellular TG levels by upregulating SREBP1c expression. HCBP6 increased SREBP1c transcription by directly binding to the SREBP1c promoter (at the -139- to +359-bp region). Moreover, we observed that HCBP6 interacted with C/EBPβ-binding site in the SREBP1c promoter both in vitro and in vivo. These results indicate that HCBP6 upregulates human SREBP1c expression by binding to the C/EBPβ-binding site in the SREBP1c promoter. [BMB Reports 2018; 51(1): 33-38]
Chang Liu,Xiaoxu Yang,Zhishan Yan,Youjun Fan,Guojun Feng,Dajun Liu 한국유전학회 2019 Genes & Genomics Vol.41 No.12
and quality. At present, little is known about the genes and molecular regulation mechanism in cold response in snap bean exposed to low temperature. Objectives Our objectives were to identify the low temperature response genes in snap bean and to examine differences in the gene response between cold-tolerant and cold-sensitive genotypes. Methods We used two highly inbred snap bean lines in this study, the cold-tolerant line ‘120’, and the cold-sensitive line ‘093’. The plants were grown to the three leaf and one heart stage and exposed to 4 °C low temperature. We used RNA sequencing (RNA-seq) to analyze the differences of gene expression. Results 988 and 874 cold-responsive genes were identified in ‘T120 vs CK120’ and ‘T093 vs CK093’ (‘T’ stands for low temperature treatment, and ‘CK’ stands for control at room temperature), respectively. Of these, 555 and 442 genes were unique to cold-stressed lines ‘120’ and ‘093’, respectively compared to the control. Our analysis of these differentially expressed genes indicates that Ca2+, ROS, and hormones act as signaling molecules that play important roles in low temperature response in P. vulgaris. Altering the expression of genes in these signaling pathways activates expression of downstream response genes which can interact with other signaling regulatory networks. This may maintained the balance of ROS and hormones, making line ‘120’ more cold-tolerant than line ‘093’. Conclusion Our results provide a preliminarily understanding of the molecular basis of low temperature response in snap bean, and also establish a foundation for the future genetic improvement of cold sensitivity in snap bean by incorporating genes for cold tolerance.
Wenwen Ding,Xiaoxue Yang,Kaiyi Lai,Yu Jiang,Ying Liu 대한약학회 2024 Archives of Pharmacal Research Vol.47 No.3
Type 2 diabetes mellitus (T2DM) is a persistent metabolic disorder marked by defi ciencies in insulin secretion and/orfunction, aff ecting various tissues and organs and leading to numerous complications. Mitochondrial biogenesis, the processby which cells generate new mitochondria utilizing existing ones plays a crucial role in energy homeostasis, glucosemetabolism, and lipid handling. Recent evidence suggests that promoting mitochondrial biogenesis can alleviate insulinresistance in the liver, adipose tissue, and skeletal muscle while improving pancreatic β -cell function. Moreover, enhancedmitochondrial biogenesis has been shown to ameliorate T2DM symptoms and may contribute to therapeutic eff ects for thetreatment of diabetic nephropathy, cardiomyopathy, retinopathy, and neuropathy. This review summarizes the intricate connectionbetween mitochondrial biogenesis and T2DM, highlighting the potential of novel therapeutic strategies targetingmitochondrial biogenesis for T2DM treatment and its associated complications. It also discusses several natural productsthat exhibit benefi cial eff ects on T2DM by promoting mitochondrial biogenesis.
Fu, Deyi,Zhao, Xiaoxu,Zhang, Yu-Yang,Li, Linjun,Xu, Hai,Jang, A-Rang,Yoon, Seong In,Song, Peng,Poh, Sock Mui,Ren, Tianhua,Ding, Zijing,Fu, Wei,Shin, Tae Joo,Shin, Hyeon Suk,Pantelides, Sokrates T.,Zho American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.27
<P>Atomically thin molybdenum disulfide (MoS2), a direct-band-gap semiconductor, is promising for applications in electronics and optoelectronics, but the scalable synthesis of highly crystalline film remains challenging. Here we report the successful epitaxial growth of a continuous, uniform, highly crystalline monolayer MoS2 film on hexagonal boron nitride (h-BN) by molecular beam epitaxy. Atomic force microscopy and electron microscopy studies reveal that MoS2 grown on h-BN primarily consists of two types of nucleation grains (0 aligned and 60 degrees antialigned domains). By adopting a high growth temperature and ultralow precursor flux, the formation of 60 degrees antialigned grains is largely suppressed. The resulting perfectly aligned grains merge seamlessly into a highly crystalline film. Large-scale monolayer MoS2 film can be grown on a 2 in. h-BN/sapphire wafer, for which surface morphology and Raman mapping confirm good spatial uniformity. Our study represents a significant step in the scalable synthesis of highly crystalline MoS2 films on atomically flat surfaces and paves the way to large-scale applications.</P>
Preparation and Drug Release Behavior of Tussah Silk Fibroin Composite Membrane
Zhuang Hu,Kaixia An,Jia Wang,Xiaoxu Xu,Zhangpei Chen,Jianshe Hu,Liqun Yang 한국섬유공학회 2020 Fibers and polymers Vol.21 No.2
Silk fibroin (SF) aqueous solution was obtained by the degumming and dissolution of tussah silk, and then the obtained solution was chemically cross-linked with sodium alginate (SA) to form composite membrane, which could be used as a carrier for sustained drug release. The mechanical properties and dissolution rate of such composite membrane togetherwith the drug release behavior under different conditions were investigated. A series of structure-tunable composite membranes were prepared through altering the ingredients proportion, the pH value during fabrication, the concentration of Ca2+, and cross-linking time. The drug release behavior of the corresponding drug-loaded composite membrane was elaborate studied using artemisinin as the model drug. It was found that the drug release rate exhibited a decreasing trend with increasing the SF content, the Ca2+ concentration and the cross-linking time of the corresponding composite membrane. Moreover, the drug release behavior exhibited pH sensitivity, and the drug release rate could be promoted under weak alkaline conditions.
Inhibition of glutathione metabolism attenuates esophageal cancer progression
Liang Peng,Ruixia Linghu,Demeng Chen,Jing Yang,Xiaoxue Kou,Xiang-Zhen Wang,Yi Hu,Yi-Zhou Jiang,Junlan Yang 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-
Esophageal squamous cell carcinoma (ESCC) is a deadly malignancy with regard to mortality and prognosis, and the 5-year survival rate for all patients diagnosed with ESCC remains poor. A better understanding of the biological mechanisms of ESCC tumorigenesis and progression is of great importance to improve treatment of this disease. In this study, we demonstrated that the glutathione metabolism pathway is highly enriched in ESCC cells compared with normal esophageal epithelial cells in an in vivo mouse model. In addition, treatment with L-buthionine-sulfoximine (BSO) to deplete glutathione decreased the ESCC tumor burden in mice, thus demonstrating the critical role of glutathione metabolism in ESCC progression. BSO treatment also led to decreased cell proliferation and activation of cell apoptosis in ESCC. Finally, BSO treatment blocked NF-kB pathway activation in ESCC. Our study reveals a new pathway that regulates ESCC progression and suggests that inhibition of glutathione metabolism may be a potential strategy for ESCC treatment
Effects of Fengycin from Bacillus subtilis fmbJ on Apoptosis and Necrosis in Rhizopus stolonifer
Qunyong Tang,Xiaomei Bie,Zhaoxin Lu,Fengxia Lv,Yang Tao,Xiaoxu Qu 한국미생물학회 2014 The journal of microbiology Vol.52 No.8
The lipopeptide antibiotic fengycin, produced by Bacillussubtilis, strongly inhibits growth of filamentous fungi. Inthis study, we evaluated the effects of fengycin treatment onapoptosis and necrosis in Rhizopus stolonifer by means ofcell staining and epifluorescence microscopy. At fengycinconcentrations less than 50 μg/ml, treated fungal cells demonstrateda dose-dependent increase in apoptosis-associatedmarkers compared with the untreated control. These markersincluded chromatin condensation, reactive oxygen speciesaccumulation, mitochondrial membrane potential depolarization,phosphatidylserine externalization, and the occurrenceof DNA strand breaks. These results showed that fungalcells were impaired in a number of important functionsand entered apoptosis upon treatment with low concentrationsof fengycin. In contrast, high concentrations (>50 μg/ml)induced necrosis, indicating that the fungicidal action offengycin operates via two modes: apoptosis at low concentrationsand necrosis at high concentrations. Additionally,the apoptotic effect that we have shown suggests that lowerconcentrations of fengycin than previously thought may beeffective for food preservation.