가시오갈피 열매 추출물의 항돌연변이원성 및 세포독성 효과

전윤영,최승필,이효진,문선영,이득식,함승시 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3

가시오갈피(Eleutherococcus senticous Maxim) 열매의 에탄올 추출물 및 분획물의 항돌연변이성 및 항암활성을 규명하였다. 항돌연별이원성 실험결과에서는 직접변이원인 MNNG, 4NQO 그리고 간접변이원인 B(α)P, Trp=P-1에 대해서 농도의존적인 돌연변이 억제활성을 나타내었다. MNNG (0.4㎍/plate)의 경우 S. typhimurium TA100 균주에서는 시료농도 200㎍/plate에서 클로로포름 분획물을 제외한 모든 분획물들에서 90%이상의 높은 억제효과를 나타내었다. 4NQO(0.15㎍/plate)에 대한 S. typhimurium TA98 균주의 경우 물 분획물의 경우 88.3%, TA100 균주에서는 에틸 아세테이트 분획물이 84.4%의 억제효과를 보였다. 간접변이원의 경우 B(α)P(10㎍/plate)에선느 에탄올 추출물과 핵산 분획물은 시료농도 200㎍/plate에서 각각 96.1%와 97.5%로 가장 높은 억제효과를 나타내었다. 그리고 Trp-P-1(0.15㎍/plate)에서는 TA100 균주의 경우 200㎍/plate 농도에서 에탄올 추출물과 물 분획물이 각각 95.5%와 90%로 높은 억제율을 보여주었고, TA98 균주에 대해서는 에틸아세티에트 분획물이 88.3%로 비교적 높은 억제효과를 나타내었다. 인간의 암세포인 폐암세포(A549), 위암세포(AGS), 간암세포(Hep3B), 유방암세포(MCF-7)에 대한 세포독성 억제 효과를 검토한 결과 모든 암세포에서 각분획물들은 1㎎/mL 농도에서 60% 이상의 비교적 높은 암세포 성장 효과를 나타내었다. 특히 MCF-7에서 높은 암세포 성장억제 효과를 나타내었는데 핵산 분획물(1㎎/mL)에서 92.7%의 높은 암세포 성장 억제 효과를 나타내었고 Hep3B에서는 부탄을 분획물(1㎎/mL)에서 82%의 비교적 높은 성장 억제효과를 나타내었다. This study was carried out to investigate the antimutagenic and cytotoxic effects of Eleutherococcus senticosus Maxim fruits ethanol extract on Salmonella typhimurium TA98, TA 100 and cancer cell lines using Ames test and SRB assay, respectively. They were extracted with ethanol and then fractionated with hexane, chloroform, ethyl acetate, butanol and water to get active fractions. In the Ames test, most of the extract had strong antimutagenic effects against the mutagenesis induced by MNNG, 4NQO, B(α)P Trp-P-1. The ethanol extract (200 ㎍/plate) of Eleutherococcus senticosus fruits showed 87.2% inhibitory effect on the mutagenesis induced by MNNG against TA100. And also, The suppression ratio against B(α)P and AGS, MCF-7, Hep3B), the value value of inhibition were mostly above 60% for each fraction (1 ㎎/mL). Hexane fraction (1 ㎎/mL) against showed the strongest cytotoxic effects of 92.7% compared to those of other fraction and butano fraetion against Hep3B was relatively high growth inhibitory effect of 82%.

다시마 분말을 첨가한 막장 에탄올 추출물의 항돌연변이원성 및 세포독성 효과

최승필,조미애,전윤영,이득식,함승시 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.1

This study was carried out to investigate antimutagenic and cytotoxic effects of Korean traditional Mackjang added with sea tangle powder. The content percentage of most of minerals among general composition increased by adding sea tangle powder. By the Ames test, the antimutagenic effect of ethanol extract of Korean traditional Mackjang added with 5% sea tangle powder was strongest, exceeding the control, 10% and 15% sea tangle additions. The ethanol extract (400 ㎍/plate) of Mackjang added with 5% sea tangle powder in the S. typhimurium TA100 strain showed inhibition rate of 95.0% against the mutagencsis induced by MNNG. The inhibition rate of ethanol extract (400 ㎍/plate) of Mackjang added with 5% sea tangle powder in the S. typhimurium TA98 and TA100 strains was 81.4% and 88,8%, respectively, against the mutagencsis induced by 4NQO. Cinder the same conditions, the suppression against B(a)P and Trp-P-1 in the TA98 and TA100 strains were 85.3% and 91.0%, and 96.5% and 92.0%. respectively. For the anticancer effects, an investigation was done on the cytotoxicity of Mackjang added with 5% sea tangle powder on the cell lines with human lung carcinoma (A549), human hepatoeellular carcinoma (HepG2), and human gastric carcinoma (KATOIII). The cytotoxicities were inhibited with increasing the extract concentration. The treatment of 1.0 ㎎/mL Mackjang added with 5% sea tangle powder showed relatively strong cytotoxicity of 61.2%, 61.8% and 66.8% against A549, KATOIII, and HepG2, respectively.

Zearalenone exposure affects epigenetic modifications of mouse eggs

Zhu, Cheng-Cheng,Hou, Yan-Jun,Han, Jun,Cui, Xiang-Shun,Kim, Nam-Hyung,Sun, Shao-Chen Oxford University Press 2014 Mutagenesis Vol.29 No.6

<P>Zearalenone (ZEA) is a mycotoxin produced by various <I>Fusarium</I> fungi, which has been shown to cause several cases of mycotoxicosis in farm animals and humans. However, there is no evidence regarding the effect of ZEA on mouse egg developmental competence. In this study, we found that the activation rate of maturated oocytes was affected in mice by ZEA treatment, indicating that ZEA affects egg developmental competence. And we explored possible mechanisms of low mouse maturated oocyte developmental competence after ZEA treatment from an epigenetic modification perspective. The fluorescence intensity analysis showed that 5-methyl cytosine level increased after ZEA treatment, indicating that the general DNA methylation level increased in the treated eggs. Moreover, histone methylations were also altered: H3K4me2 as well as H3K9me3 and H4K20me1, me2, me3 levels decreased in eggs that were cultured in high-dose ZEA medium. Thus, our results indicated that ZEA decreased egg developmental competence by affecting the epigenetic modifications.</P>

A new approach for predicting wear overlap geometry in ball end finish milling

Cheng, De-Jun,Oh, Young-Jin,Kim, Su-Jin Springer-Verlag 2018 INTERNATIONAL JOURNAL OF ADVANCED MANUFACTURING TE Vol.98 No.9

Prevent disease of COVID-19

Jun Cheng Yin 한국간호과학회 2023 한국간호과학회 학술대회 Vol.2023 No.11

An Asynchronous Operation Approach to Event-Triggered Control for Fuzzy Markovian Jump Systems With General Switching Policies

Cheng, Jun,Park, Ju H.,Zhang, Lixian,Zhu, Yanzheng Institute of Electrical and Electronics Engineers, 2018 IEEE transactions on fuzzy systems Vol.26 No.1

<P>This paper investigates the problem of event-triggered control for a class of fuzzy Markov jump systems with general switching policies. A novel event-triggered scheme is proposed to improve the transmission efficiency at each sampling instance. Each transition rate allows to be unknown, known, or only its uncertain domains value is known. With the help of a tailored technique to bind the uncertain terms and an asynchronous operation approach to tackle the fuzzy system and fuzzy controller, sufficient conditions for the resulting fuzzy Markovian jump systems are established in terms of coupled linear matrix inequalities. Finally, an example is given to illustrate the validity of the developed technique.</P>

Finite-time Boundness of H∞ Filtering for Switching Discrete-Time Systems

Jun Cheng,Hong Zhu,Shouming Zhong,Yu-Ping Zhang 제어·로봇·시스템학회 2012 International Journal of Control, Automation, and Vol.10 No.6

For switched discrete-time systems, switching behavior always affect the finite-time stability property, which was neglected by most previous research. This paper investigated the problem of finite-time boundness of H∞ filtering for switched discrete-time systems. Sufficient conditions which can ensure finite-time bounded and H∞ filtering finite-time boundness under arbitrary switching are derived. Based on the results of finite-time boundness and stochastic character, the closed-loop system trajectory stays within a prescribed bound. An example is given to illustrate the efficiency of the proposed method.

(m,N)-delay-partitioning Approach to stability Analysis for T-S Fuzzy systems with Interval Time-varying Delay

Jun Yang,Wenpin Luo,Yong-Hu Wang,Jun Cheng 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.2

This paper is concerned with improved stability criteria for uncertain T-S fuzzy systems with intervaltime-varying delay by means of a new (m;N)-delay-partitioning approach. Based on an appropriate augmented LKFestablished in the framework of state vector augmentation, some tighter bounding inequalities (Seuret-Wirtinger’sintegral inequality, Peng-Park’s integral inequality and the reciprocally convex approach) have been employed todeal with (time-varying) delay-dependent integral items of the derivative of LKF, therefore, less conservative delaydependentstability criteria can be obtained on account of none of any useful time-varying items are arbitrarilyignored. It’s worth mentioning that, when the delay-partitioning number m is fixed, less conservatism can beachieved by increase of another delay-partitioning number N, but without increasing any computing burden. Finally,one numerical example is provided to show that the proposed conditions are less conservative than existing ones.

Effect of hanging-type sand fence on characteristics of wind-sand flow fields

Cheng, Jian-jun,Lei, Jia-qiang,Li, Sheng-yu,Wang, Hai-feng Techno-Press 2016 Wind and Structures, An International Journal (WAS Vol.22 No.5

A hanging-type sand-retaining wall is a very common sand-blocking fence structure used to prevent sand movement. This type of wall is widely used along the Qinghai-Tibet and Gobi desert railways in Xinjiang, Western China. To analyze the characteristics of wind-sand flow fields under the effect of such a sand fence structure, a wind tunnel test and a field test were carried out. The wind tunnel test showed the zoning characteristics of the flow fields under the effect of the hanging-type sand-retaining wall, and the field test provided the sediment transport data for effective wind-proof interval and the sand resistance data in the front and behind the sand-retaining wall. The consistency of the wind-sand flow fields with the spatial distribution characteristic of wind-carried sand motion was verified by the correspondences of the acceleration zone in the flow field and the negative elevation points of the percentage variations of the sand collection rate. The spatial distribution characteristic of the field sand collection data further showed the spatial structural characteristic of the sandy air currents under the action of the hanging-type sand-retaining wall and the sand resistance characteristic of the sand-retaining wall. This systematic study on the wind-sand flow fields under the control of the hanging-type sand-retaining wall provides a theoretical basis for the rational layout of sand control engineering systems and the efficient utilization of a hanging-type sand-retaining wall.

Improving the CO2 fixation rate by increasing flow rate of the flue gas from microalgae in a raceway pond

Jun Cheng,Zongbo Yang,Junhu Zhou,Kefa Cen 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.2

Residence time of flue gas bubbles with different solution velocities and the influence of NOX and SO2 from flue gas on pH values of culture solutions were analyzed based on large-scale raceway reactors. Microalgal growth and CO2 fixation rates were also investigated with different gas flow rates. Bubble residence time was ~1.1 s when the solution velocity was 20 cm/s. The NOX and SO2 effects on microalgal growth were negligible, although 66% NOX and 95% SO2 were captured by the microalgal solution. Microalgal biomass productivity increased from 10.3 to 14.1 g/m2/d when flue gas flow rate increased from 20 to 50m3/h. CO2 fixation and microalgae biomass productivity increased further from 26.3 to 31.9 g/m2/d and from 14.1 to 17.1 g/m2/d, respectively, upon increase of flue gas flow rate from 50 to 150m3/h.