Research of Product Design Optimization Based on Supporting Vector and Artificial Fish Swarm Algorithm

Yongqiang Li 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.7

Exploration Analysis Based on Improved BP Neural Network in Human Body Movement

Yongqiang Li 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.7

Research on Human Face Difference Imaging Based on Sparse Representation

Yongqiang Li 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.7

To make sure human face can be more accurately identified in various poses, an identification method based on the characteristics human face image have been proposed in the thesis. First, it is a design for the optimal sampling matrix to acquire compressed measurement data; then it adopts iterative method based on the close loops of l2 and l1 in normal form to get an estimate on human face images, since the method based on norm l2 can measure the relevancy of human face images in the space and time of the continuous time point, and the method based on norm l1 mainly uses the modified total variation method and basis pursuit noise-reduction method. The simulation design has suggested that the method adopted in the thesis can achieve a higher human face identification rate and a remarkable promotion effect.

Iris Recognition Algorithm based on MMC-SPP

Yongqiang Li 보안공학연구지원센터 2015 International Journal of Signal Processing, Image Vol.8 No.2

The WSN Coverage Optimization of the Diversified AFSABased on ChaosLearning Strategy

Yongqiang Li,PanJin 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.8

Neutrophil-inspired photothermo-responsive drug delivery system for targeted treatment of bacterial infection and endotoxins neutralization

Chengnan Li,Yingying Gan,Zongshao Li,Mengjing Fu,Yuzhen Li,Xinran Peng,Yongqiang Yang,Guo‑bao Tian,Yi Yan Yang,Peiyan Yuan,Xin Ding 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background P. aeruginosa, a highly virulent Gram-negative bacterium, can cause severe nosocomial infections, and it has developed resistance against most antibiotics. New therapeutic strategies are urgently needed to treat such bacterial infection and reduce its toxicity caused by endotoxin (lipopolysaccharide, LPS). Neutrophils have been proven to be able to target inflammation site and neutrophil membrane receptors such as Toll-like receptor-4 (TLR4) and CD14, and exhibit specific affinity to LPS. However, antibacterial delivery system based on the unique properties of neutrophils has not been reported. Methods A neutrophil-inspired antibacterial delivery system for targeted photothermal treatment, stimuli-responsive antibiotic release and endotoxin neutralization is reported in this study. Specifically, the photothermal reagent indocyanine green (ICG) and antibiotic rifampicin (RIF) are co-loaded into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NP-ICG/RIF), followed by coating with neutrophil membrane to obtain antibacterial delivery system (NM-NP-ICG/RIF). The inflammation targeting properties, synergistic antibacterial activity of photothermal therapy and antibiotic treatment, and endotoxin neutralization have been studied in vitro. A P. aeruginosa-induced murine skin abscess infection model has been used to evaluate the therapeutic efficacy of the NM-NP-ICG/RIF. Results Once irradiated by near-infrared lasers, the heat generated by NP-ICG/RIF triggers the release of RIF and ICG, resulting in a synergistic chemo-photothermal antibacterial effect against P. aeruginosa (~ 99.99% killing efficiency in 5 min). After coating with neutrophil-like cell membrane vesicles (NMVs), the nanoparticles (NM-NP-ICG/RIF) specifically bind to inflammatory vascular endothelial cells in infectious site, endowing the nanoparticles with an infection microenvironment targeting function to enhance retention time. Importantly, it is discovered for the first time that NMVs-coated nanoparticles are able to neutralize endotoxins. The P. aeruginosa murine skin abscess infection model further demonstrates the in vivo therapeutic efficacy of NM-NP-ICG/RIF. Conclusion The neutrophil-inspired antibacterial delivery system (NM-NP-ICG/RIF) is capable of targeting infection microenvironment, neutralizing endotoxin, and eradicating bacteria through a synergistic effect of photothermal therapy and antibiotic treatment. This drug delivery system made from FDA-approved compounds provides a promising approach to fighting against hard-to-treat bacterial infections.

Histone demethylase KDM4A plays an oncogenic role in nasopharyngeal carcinoma by promoting cell migration and invasion

Zhao Jingyi,Li Bingyan,Ren Yongxia,Liang Tiansong,Wang Juan,Zhai Suna,Zhang Xiqian,Zhou Pengcheng,Zhang Xiangxian,Pan Yuanyuan,Gao Fangfang,Zhang Sulan,Li Liming,Yang Yongqiang,Deng Xiaoyu,Li Xiaole,C 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Compelling evidence has indicated the vital role of lysine-specific demethylase 4 A (KDM4A), hypoxia-inducible factor-1α (HIF1α) and the mechanistic target of rapamycin (mTOR) signaling pathway in nasopharyngeal carcinoma (NPC). Therefore, we aimed to investigate whether KDM4A affects NPC progression by regulating the HIF1α/DDIT4/mTOR signaling pathway. First, NPC and adjacent tissue samples were collected, and KDM4A protein expression was examined by immunohistochemistry. Then, the interactions among KDM4A, HIF1α and DDIT4 were assessed. Gain- and loss-of-function approaches were used to alter KDM4A, HIF1α and DDIT4 expression in NPC cells. The mechanism of KDM4A in NPC was evaluated both in vivo and in vitro via RT-qPCR, Western blot analysis, MTT assay, Transwell assay, flow cytometry and tumor formation experiments. KDM4A, HIF1α, and DDIT4 were highly expressed in NPC tissues and cells. Mechanistically, KDM4A inhibited the enrichment of histone H3 lysine 9 trimethylation (H3K9me3) in the HIF1α promoter region and thus inhibited the methylation of HIF1α to promote HIF1α expression, thus upregulating DDIT4 and activating the mTOR signaling pathway. Overexpression of KDM4A, HIF1α, or DDIT4 or activation of the mTOR signaling pathway promoted SUNE1 cell proliferation, migration, and invasion but inhibited apoptosis. KDM4A silencing blocked the mTOR signaling pathway by inhibiting the HIF1α/DDIT4 axis to inhibit the growth of SUNE1 cells in vivo. Collectively, KDM4A silencing could inhibit NPC progression by blocking the activation of the HIF1α/DDIT4/mTOR signaling pathway by increasing H3K9me3, highlighting a promising therapeutic target for NPC.

Structural evolution and kinetic study of high isotacticity poly(acrylonitrile) during isothermal pre-oxidation

Li Zhang,Yongqiang Dai,Yi Kai,Ri-Guang Jin 한국탄소학회 2011 Carbon Letters Vol.12 No.4

Isotactic polyacrylonitrile (PAN) with triad isotacticity of 0.53, which was determined by 13C NMR, using dialkylmagnesium as an initiator, was successfully synthesized. Isothermal treatment of iso-PAN was conducted in air at 200, 220, 250 and 280℃. Structural evolutions and chemical changes were studied with Fourier transformation infrared and wide-angle X-ray diffraction during stabilization. A new parameter CNF=I2240cm-1/ (I1595cm-1+f*I1595cm-1) was defined to evaluate residual nitrile groups. Crystallinity and crystal size were calculated with X-ray diffraction dates. The results indicated that the nitrile groups had partly converted into a ladder structure as stabilization proceeded. The rate of reaction increased with treatment temperature; crystallinity and crystal size decreased proportionally to pyrolysis temperature. The iso-conversional method coupled with the Kissinger and Flynn-Wall-Ozawa methods were used to determine kinetic parameters via differential scanning calorimetry analysis with different heating rates. The active energy of the reaction was 171.1 and 169.1 kJ/mol, calculated with the two methods respectively and implied the sensitivity of the reaction with temperature.

Inter-ramet Physiological Integration Detected in Buffalograss (Buchloe dactyloides (Nutt.) Engelm.) under Water Stress

Yongqiang Qian(뀐용퀴앙),Deying Li(이다이엥),Lei Han(한레이),Guansheng Ju(주관쉥),Junxiang Liu(류준샹),Juying Wu(위주잉),Zhenyuan Sun(선젠얀) 한국잡초학회·한국잔디학회 2009 Weed & Turfgrass Science Vol.23 No.2

Buffalograss는 내한, 내서, 그리고 내한발에 우수한 주요 잔디 종 중 하나이다. 다양한 환경에서 buffalograss의 생리학적 조정(integration)을 이해하는 것은 균일한 잔디의 질을 도모하고 경종적 재배방법의 개발에 도움이 된다. 본 연구의 목적은 물 부족의 스트레스 처리에서의 buffalograss의 생리학적 조정과정에서 lipid peroxidation과 산화방지제의 연관성을 평가하였다. 한 실험에서 buffalograss는 네 개의 구분된 칸막이 성장 유닛의 중심에서 재배되었고, 일주일에 한번(+), 일주일에 두 번(-) 관수처리와 모래(S) 또는 피트(P)와를 혼합한 다섯 가지 토양 조합으로 처리하였다(P+S-P-S+, P+P+P+P+, S-S-S-S-, P-P-P-P-, and S+S+S+S+). 그 결과, 균일하게 혼합된 상토에서 생장한 줄기의 수가 네개의 단일 상토에 정착한 것보다 더 많았다. 두 번째 실험에서는 Hoagland 용액(S?), 또는 20% PEG-6000이 함유된 Hoagland용액(Ss)안에 하나의 라미트(무성생식체) 혹은 연결된 라마트를 다음과 같은 여러 가지 처리와 비교 실험하였다. 연결된 라미트들의 처리는 Hoagland 용액안의 어린 라미트(Yos)와 20%PEG-6000가 함유된 Hoagland 용액안의 성숙한 라미트(Oos), Hoagland 용액 단독에 성숙한 라미트(Oys), 20%PEG-6000 함유된 Hoagland 용액안의 어린 라미트(Yys)였다. Lipid peroxidation, antioxidants, proline은 각기 다른 수분 stress 정도에서 라미트들 간의 생리학적 활성을 보여주었다. Superoxide dismutase (SOD), Guaiacol peroxidase (G-POD), malondi aldehyde (MDA), free proline의 활성도 처리 후 시간에 따라 상대적인 생리학적 활성을 보였다. Buffalograss is an important turfgrass species with excellent cold, heat, and drought tolerance. Understanding the physiological integration of buffalograss under heterogeneous conditions helps to develop cultural practices that better use limited resources for uniform turf quality. The objective of this study was to evaluate physiological integration of buffalograss under water deficit stress and the involvement of lipid peroxidation and antioxidants in the process. In one experiment, buffalograss was planted in the center of a four-compartment growth unit. Watering frequencies, once a week(+) and once in two weeks(-), were combined with the sand(S) or peat(P) in each unit to generate five total treatments (P+S-P-S+, P+P+P+P+, S-S-S-S-, P-P-P-P-, and S+S+S+S+). The average number of shoot established from the heterogeneous root-zone medium was higher than the average of four possible homogeneous media. In second experiment, single ramet in Hoagland solution(S?) or single ramet in Hoagland solution with 20% PEG-6000(Ss) were compared with two connectedramets under different treatments. Treatments for connected ramets were young ramet in Hoagland solution(Yos) and old ramet in Hoagland solution with 20% PEG-6000(Oos), and old ramet in Hoagland solution(Oys) and young ramet in Hoagland solution with 20% PEG-6000(Yys). Lipid peroxidation, antioxidants, and proline showedphysiological integration between ramets subjected to different levels of water stress. Superoxide dismutase(SOD), Guaiacol peroxidase(G-POD), malondialdehyde(MDA), and free proline also showed different time courses and relative activities during the physiological integration.

In vivo and in vitro evaluation of stability and antioxidant activity of lycopene-nanostructured lipid carriers

Yongqiang Ma,Chenchen Li,Weiye Xiu,Xin Wang 한국식품과학회 2023 Food Science and Biotechnology Vol.32 No.6

This study evaluates the stability of lycopene in the presence of the prepared nanostructured lipid carriers (NLCs) under different environments and food systems and the in vitro and in vivo antioxidant activity of the lycopene nanostructured lipid carriers (Lyco-NLCs) was studied. As observed in the stability experiment, Lyco-NLCs have good storage stability within 30 days. Food additives have little effect on its stability except for metal ions. Compared with free lycopene, Lyco-NLCs showed an improved antioxidant property. In in-vitro experiments, the DPPH radical scavenging rate, hydroxyl radical scavenging capacity, and ferric reducing capacity of Lyco-NLCs increased by 90.47%, 47.43%, and 45.12%, respectively. The animal experiments showed that the activities of catalase in the kidney, superoxide dismutase in the heart, and glutathione peroxidase in the liver increased by 31.48%, 42.50%, and 21.47%, respectively. The content of malondialdehyde in serum decreased by 14.13%. The results have some significance for the practical application of lycopene.