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Xiaohui Shan,Xiaoyu Wang,Guang Yang,Ying-Wu Lan,Shengzhong Su,Shipeng Li,Hongkui Liu,Yaping Yuan 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
DNA methylation plays a vital role in tuning geneexpression in response to environmental stimuli. Here,methylation-sensitive amplified polymorphisms (MSAP)were used to assess the effect of cold stress on the extent andpatterns of DNA methylation in maize seedlings. Overall,cold-induced genome-wide DNA methylation polymorphismsaccounted for 32.6 to 34.8% of the total bands at the differenttreatment time-points. It was demonstrated that the extentand pattern of DNA methylation was induced by cold stressthrough the cold treatment process and that thedemethylation of fully methylated fragments was the maincontributor of the DNA methylation alterations. The sequencesof 28 differentially amplified fragments relevant to stresswere successfully obtained. Under the cold stress, demethylationwas detected in most fragments. BLAST results indicate thatthe homologues of these fragments are involved in manyprocesses, including hormone regulation, cold response,photosynthesis, and transposon activation. The expressionanalysis demonstrated an increase in the transcription of fivedemethylated genes. Despite the fact that DNA methylationchanges and cold acclimation are not directly associated, ourresults may indicate that the specific demethylation of genesis an active and rapid epigenetic response to cold in maizeduring the seedling stage, further elucidating the mechanismof maize adaptation to cold stress.
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Hussaini Abdullahi Umar,Xiaohui Zeng,Xuli Lan,Huasheng Zhu,Yirui Li,Haichuan Liu 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.12
Filling layer self-compacting concrete (FLSCC) is an essential material used in the construction of China Rail Track System type III (CRTS III) slab track of high-speed rail. The mechanical properties of the filling layer are of paramount significance to the performance and serviceability of the track system. In this study, asphalt emulsion (AE) was incorporated into the FLSCC, and various properties related to workability, strength, durability, and damping performance were experimentally evaluated. The effect of AE on the durability of FLSCC was investigated through water absorption, sorptivity, and rapid chloride ion penetration tests; while damping properties were evaluated using impact resonance method. Results show that addition of AE decreases the compressive and split tensile strength while flexural strength increases, especially at lower AE content. The durability properties were greatly enhanced with the addition of AE, which results from the decreased permeability of FLSCC specimens due to the pore-filling effect of asphalt film that flows and fills the pores and cracks of the matrix. Damping ratio of FLSCC increased by 22.8%, 23.7%, 25%, and 30.6% through the addition of 5%, 10%, 15%, and 20% AE, respectively. According to the findings of this study, AE content up to 10% is recommended for FLSCC production.
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Shuang Li,Bo Wang,Shun Jiang,Xiaohui Lan,Yongbo Qiao,Jiaojiao Nie,Yuhe Yin,Yuhua Shi,Wei Kong,Yaming Shan 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.1
Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vivo after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions: The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.
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