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( Xia Zhong ),( Shaomin Yang ),( Xinying Su ),( Xiaoxia Shen ),( Wenzhao ),( Zhi Chan ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5
Nitrilase is a valuable hydrolase that catalyzes nitriles into carboxylic acid and ammonia. Its applications, however, are severely restricted by the harsh conditions of industrial reaction processes. To solve this problem, a nitrilase from Acidovorax facilis 72W was inserted into an Escherichia coli-Bacillus subtilis shuttle vector for spore surface display. Western blot, enzyme activity measurements and flow cytometric analysis results all indicated a successful spore surface display of the CotB-nit fusion protein. In addition, the optimal catalytic pH value and temperature of the displayed nitrilase were determined to be 7.0 and 50°C, respectively. Moreover, results of reusability tests revealed that 64% of the initial activity of the displayed nitrilase was still retained at the 10th cycle. Furthermore, hydrolysis efficiency of upscale production of cyanocarboxylic acid was significantly higher in the displayed nitrilase-treated group than in the free group expressed by E. coli (pET-28a-nit). Generally, the display of A. facilis 72W nitrilase on the spore surface of Bacillus subtilis may be a useful method for immobilization of enzyme and consequent biocatalytic stabilization.
Shotgun analysis on the peritrophic membrane of the silkworm Bombyx mori
( Xiao Wu Zhong ),( Li Ping Zhang ),( Yong Zou ),( Qi Ying Yi ),( Ping Zhao ),( Qing You Xia ),( Zhong Huai Xiang ) 생화학분자생물학회 2012 BMB Reports Vol.45 No.11
The insect midgut epithelium is generally lined with a unique chitin and protein structure, the peritrophic membrane (PM), which facilitates food digestion and protects the gut epithelium. We used gel electrophoresis and mass spectrometry to identify the extracted proteins from the silkworm PM to obtain an in-depth understanding of the biological function of the silkworm PM components. A total of 305 proteins, with molecular weights ranging from 8.02 kDa to 788.52 kDa and the isoelectric points ranging from 3.39 to 12.91, were successfully identified. We also found several major classes of PM proteins, i.e. PM chitin-binding protein, invertebrate intestinal mucin, and chitin deacetylase. The protein profile provides a basis for further study of the physiological events in the PM of Bombyx mori.
Study on Anharmonic Effect of the Unimolecular Reaction of CH<sub>2</sub>(D<sub>2</sub>)FO
Zhong, Jingjun,Li, Qian,Luo, Ji,Xia, Wenwen,Yao, Li,Lin, S.H. Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.12
Study on the unimolecular reaction for $CH_2FO$ and $CD_2FO$ is carried out. The structures, energy barriers and zero point energy of the three channels in the title unimolecular reactions are computed with the MP2/6-311++G(3df, 3pd) method. RRKM theory is used to calculate the rate constants of canonical case at temperature range of 500-5000 K and microcanonical system at total energy of 19.05-71.68 kcal/mol. The results indicate that the anharmonic effect and isotope effect are very small for the three channels, and the anharmonic rate constants, around $10^9-10^{11}s^{-1}$, are close to the experimental prediction reasonably.
Xia-Wa Mao,Jia-Quan Xiao,Zhong-Yi Li,Yi-Chun Zheng,Nan Zhang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
This study investigated the effects of microRNA-135a (miR-135a) targeting of glycogen synthase kinase 3β (GSK3β) on the epithelial–mesenchymal transition (EMT), migration and invasion of bladder cancer (BC) cells by mediating the Wnt/β-catenin signaling pathway. BC and adjacent normal tissues were collected from 165 BC patients. Western blotting and quantitative realtime PCR were used to detect the expression of GSK3β, β-catenin, cyclinD1, E-cadherin, vimentin and miR-135a in BC tissues and cells. Cells were assigned to blank, negative control (NC), miR-135a mimics, miR-135a inhibitors, small interfering RNA (siRNA)-GSK3β or miR-135a inhibitors+siRNA-GSK3β groups. miR-135a, β-catenin, cyclinD1 and vimentin expression increased, while GSK3β and E-cadherin expression decreased in BC tissues compared with adjacent normal tissues. Compared with the blank and NC groups, the expression of miR-135a, β-catenin, cyclinD1 and vimentin was higher, and cell proliferation, migration, invasion and tumor growth were increased in the miR-135a mimics and siRNA-GSK3β groups. These groups showed an opposite trend in GSK3β and E-cadherin expression and cell apoptosis. The miR-135a inhibitors group was inversely correlated with the blank and NC groups. It was concluded that miR-135a accelerates the EMT, invasion and migration of BC cells by activating the Wnt/β-catenin signaling pathway through the downregulation of GSK3β expression.
COMPREHENSIVE ASSESSMENT MODEL OF ECOLOGICAL RIPARIAN ZONE
Xia, Ji-Hong,Wu, Wei,Yan, Zhong-Min Korea Water Resources Association 2005 Water engineering research Vol.6 No.4
Comprehensive assessment of ecological riparian zone is to analyze and evaluate the status of riparian zone ecosystem. The existing problem of the ecosystem can be found through the assessment. The AHP-FUZZY method used in the assessment is based on the hierarchy model of index, grade model of object, and attribution degree of index. Accordingly, the four models have been discussed and presented from the aspect of the stability, landscape, eco-health and eco-safety of riparian zone.
Xia, Yong-Hua,Li, Min,Fu, Dan-Dan,Xu, Su-Ling,Li, Zhan-Guo,Liu, Dong,Tian, Zhong-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11
Aims: To study effects of down-regulation of pituitary tumor-transforming gene (PTTG) on proliferation and metastasis ability of the SCL-1 cutaneous squamous cell carcinoma (CSCC) cell line and explore related mechanisms. Methods: SCL-1 cells were divided into 3 groups (untreated, siRNA control and PTTG siRNA). Cell proliferation assays were performed using a CCK-8 kit and proliferation and metastasis ability were analyzed using Boyden chambers. In addition, expression of MMP-2 and MMP-9 was detected by r-time qPCR and Western blotting. Results: Down-regulation of PTTG could markedly inhibit cell proliferation in SCL-1 cells, compared to untreated and control siRNA groups (P < 0.05). Real-time qPCR demonstrated that expression levels of PTTG, MMP-2 and MMP-9 in the PTTG siRNA group were 0.8%, 23.2% and 21.3% of untreated levels. Western blotting revealed that expression of PTTG, MMP-2 and MMP-9 proteins in the PTTG siRNA group was obviously down-regulated. The numbers of migrating cells ($51.38{\pm}4.71$) in the PTTG siRNA group was obviously lower than that in untreated group ($131.33{\pm}6.12$) and the control siRNA group ($127.72{\pm}5.20$) (P < 0.05), suggesting that decrease of proliferation and metastasis ability mediated by PTTG knock-down may be closely correlated with down-regulation of MMP-2 and MMP-9 expression. Conclusion: Inhibition of PTTG expression may be a new target for therapy of CSCC.
Xia, Wenwen,Liu, Yonglu,Zhong, Haiyang,Yao, Li Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
In this paper, the reactive dynamics properties of the reactions Ba + $C_6H_5Br$ and Ba + m-$C_6H_4CH_3Br$ were studied by means of the quasi-classical trajectory method based on the London-Eyring-Polanyi-Sato potential energy surfaces. The vibrational distributions, reaction cross sections, rotational alignments of the products BaBr all were obtained. The peak values of the vibrational distributions are located at $\nu$ = 0 for the reactions Ba + $C_6H_5Br$ and Ba + m-$C_6H_4CH_3Br$ when the collision energies are 1.09 and 1.10 eV, respectively. The reaction cross sections increase with the increasing collision energy, which changes from 0.6 to 1.5 eV. The product rotational alignments deviate from -0.5 and firstly increase and then decrease while the collision energy is increasing, just like that of Heavy+Light-Light system.
Zhong-Ying Miao,Qing-Xia Xu 한국지질과학협의회 2017 Geosciences Journal Vol.21 No.3
The Mohe Formation is one of the important source rocks for oil-gas and gas hydrates in the Mohe Basin. In this paper, mudstone from cores of the M-1 and M-3 wells and argillite from cores of the MK-2 well of the Mohe Basin were studied. The depositional environment, organic matter source, thermal maturity, kerogen type, and hydrocarbon generation potential of the core samples were determined using organic and inorganic geochemistry. The dual sources of organic matter were terrestrial plants and aquatic organisms. The organic matter was deposited in a reducing lacustrine environment. The lake water salinity was higher when argillite was deposited. The late stage of the sedimentary filling of the lacustrine basin was characterized by high sulfate contents. The source rocks primarily contained Type II2–III kerogen. The present-day thermal maturity parameters reveal that the organic matter of the Mohe Formation is mature to overmature, indicating that this unit is capable of generating gas condensate and dry gas. In addition, we compared the organic geochemistry characteristics of mudstone and argillite. The results indicate that TOC, S1 + S2, and HI are lower in argillite than in mudstone. Although the biomarker maturity parameters cannot reflect the thermal evolution of the source rocks, they reflect the source of the organic matter and the depositional environment. The results provide important constraints for the evaluation of the hydrocarbon potential of lacustrine source rocks of the Mohe Formation from the Upper Jurassic and for the location of petroleum resources in the Mohe Basin.