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Yingzhong, Yang,Droma, Yunden,Guoen, Jin,Zhenzhong, Bai,Lan, Ma,Haixia, Yun,Yue, Cao,Kubo, Keishi,Rili, Ge Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.3
To investigate the possible mechanisms of high-altitude native animals in adapting to high altitude, we cloned hemoglobin alpha-chain (alpha-chain Hb) gene from Pantholops hodgsonii, an animal species that indigenously lives at elevations of 3700-5500 m on the Qinghai-Tibetan plateau. Using reverse transcription polymerase chain reaction (RT-PCR) technique, the alpha-chain Hb gene was amplified from total RNA in the liver of the Pantholops hodgsonii. TA cloning technique was used and the PCR product was cloned into pGEM-T vector. The DNA sequence of the gene was highly homologous with sheep (99.1%), goat (98.6%), cattle (95.6%) and human (86.5%). The alpha-chain Hb gene encoded a 142-amino acid protein that could be identified with the homology of alpha-chain Hb protein in sheep (98%), goat (96%), cattle (91%) and human (87%). However, 18 alternations were detected when compared with the alpha-chain Hb gene in human, and 2 in sheep. Moreover, the alterations of a117 GluAsp and $\alpha$132 AsnSer in important regions were noted in human and sheep, respectively. Phylogenetic analysis suggested that the structure of alpha-chain Hb was highly similar to that in sheep. This study provided essential information for elucidating the possible roles of hemoglobin in adapting to extremely high altitude in Pantholops hodgsonii.
Novel flexible HVDC transmission converter station topology with DC fault blocking capability
Huo, Qunhai,Wang, Peng,Cao, Guoen,Zhu, Jin,Yin, Jingyuan,Guo, Xinming,Wei, Tongzhen The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.4
The conventional half-bridge submodule (HBSM)-based multilevel modular converter (MMC) cannot block DC faults. To solve this problem, a novel flexible overhead-line high-voltage direct current (HVDC) transmission converter station topology is proposed in this study, which provides DC fault blocking capability. By adding blocking submodules (SMs) onto the positive and negative DC buses of a conventional HBSM converter station, a rapid fault current blocking can be achieved in the case of a DC bus short circuit. The DC fault blocking principle of the proposed topology and the rapid fault blocking capability of the blocking SM are analyzed. The quantity of required major components is also calculated. In addition, the IGBT, the system loss and the control complexity quantities are comprehensively compared with those of existing topologies. The validity of the proposed topology is demonstrated based on simulation and experimental studies.