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      • Study on Forage Oat in Qinghai Alpine Region

        Yajun Ji,Qingping Zhou,Hongbo Yan,Wenhui Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

        The cultivation of oat in China could be traced back to 2500 B.C. while the Qinghai province, which located in the North-East of the Qinghai-Tibetan Plateau, has been the major region for oat production due to its high quality caused by the unique climate say long sun-shine hours and large temperature discrepancy between days and nights that is beneficial to the accumulation of nutrients in oat plant. Oat has been grown in the Qinghai alpine region on the altitude ranging from 1600m to 3600m and regarded as an ideal forage plant due to its resistance to environment. The role of oat drew more attention in 1980s along with the implementing of so called four-matched policy, which compounds establishment of fenced rangeland, animal bars, fodder production base and settled living place for local herdsmen who used to be nomadic around the region. To make a full use of the oat resource in Qinghai province, a great deal research and extension with respect of oat had been engaged in the region. The author overviewed researches accomplished in Qinghai province on oat germplasm collection, breeding of fine oat variety, cultivate techniques, oat base establishment and production and marketing system establishment as well in terms of projects completed.

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        Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine

        Fang Yan,Zhong Li,Yongting Hu,Jianyang Qiu,Wenxin Lei,Wenhui Ji,Xuying Li,Qian Wu,Xiumin shi,Yujun Zhao 대한수의학회 2013 Journal of Veterinary Science Vol.14 No.1

        The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody titers of the chickens immunized with pVAX1-16S1/M/N were much higher than those of the monovalent groups (p < 0.01). A protective rate up to 90% was observed in the pVAX1-16S1/M/N group. The serum antibody titers in the prime-boost birds were significantly higher than those of the multivalent DNA vaccine group (p < 0.01) but not significantly different compared to the inactivated vaccine group at 49 days of age. Additionally, the prime-boost group also showed the highest level of IBV-specific cellular proliferation compared to the monovalent groups (p < 0.01)but no significant difference was found compared to the multivalent DNA vaccine group, and the prime-boost group completely protected from followed viral challenge.

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