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      • Dynamics in Biomass of Poa pratensis ver. anceps Gaud. Cv. in the Qinghai-Tibetan Plateau

        Qingping Zhou,Wenhui Liu,Hongbo Yan,Guoling Liang 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

        Dynamics of aboveground and underground biomass of Qinghai flat-stem bluegrass (Poa pratensis vayr. anceps Gaud. Cv in ">" shape, and vertical distributions of biomass of roots, rhizomes and underground were all in "T" shape and fit equation of Y= l-βD. There was direct correlation between biomass of above ground and biomass of underground. Ration of underground to aboveground was 0.4l.

      • Study on Forage Oat in Qinghai Alpine Region

        Yajun Ji,Qingping Zhou,Hongbo Yan,Wenhui Liu 한국초지조사료학회 2009 한국초지조사료학회 학술대회논문집 Vol.2009 No.08

        The cultivation of oat in China could be traced back to 2500 B.C. while the Qinghai province, which located in the North-East of the Qinghai-Tibetan Plateau, has been the major region for oat production due to its high quality caused by the unique climate say long sun-shine hours and large temperature discrepancy between days and nights that is beneficial to the accumulation of nutrients in oat plant. Oat has been grown in the Qinghai alpine region on the altitude ranging from 1600m to 3600m and regarded as an ideal forage plant due to its resistance to environment. The role of oat drew more attention in 1980s along with the implementing of so called four-matched policy, which compounds establishment of fenced rangeland, animal bars, fodder production base and settled living place for local herdsmen who used to be nomadic around the region. To make a full use of the oat resource in Qinghai province, a great deal research and extension with respect of oat had been engaged in the region. The author overviewed researches accomplished in Qinghai province on oat germplasm collection, breeding of fine oat variety, cultivate techniques, oat base establishment and production and marketing system establishment as well in terms of projects completed.

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        3D Printing of Bioinspired Structural Materials with Fibers Induced by Doctor Blading Process

        Luquan Ren,Bingqian Li,Zhengyi Song,Qingping Liu,Lei Ren,Xueli Zhou 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.6 No.1

        Fiber is a crucial element in biological micro-structural materials. Replication of fiber-reinforced composites with analogous architectures of their natural counterparts has caused widespread academic concern. Recent researches indicate 3D printing technology has the potential to produce biomimetic structural materials. The aim of this study is to develop a process to fabricate fiber-reinforced composites with ordered yet spatially tunable fiber arrangement. Specifically, we present a method to align fibers during the 3D printing of fiber-reinforced composites. A modified slurry-based stereolithography process was developed, and the fibers in the fiber–resin mixture were aligned by Shear force produced during the spreading of slurry. We investigated the influence of relative factors on fiber orientation, and two models were used to uncover the internal mechanism. By controlling the speed and the direction of the moving blade, the patterns that fibers were arranged can be freely programmed. Therefore, we have extracted bioinspired sinusoidal and zigzag design motifs to analyze their mechanical properties compared with non-bioinspired motifs. The proposed method is relatively material agnostic, more efficient and more facile. It thus provides a promising route to fabricate fiber-reinforced composites, and has potential to be adopted in biological structures researches and industrial applications.

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        Norepinephrine/β2-Adrenergic Receptor Pathway Promotes the Cell Proliferation and Nerve Growth Factor Production in Triple-Negative Breast Cancer

        Meihua Jin,Yan Wang,Tingting Zhou,Wenzhe Li,Qingping Wen 한국유방암학회 2023 Journal of breast cancer Vol.26 No.3

        Purpose: Invasive ductal carcinoma (IDC) accounts for 90% of triple-negative breast cancer (TNBC). IDC is mainly derived from the breast ductal epithelium which is innervated by the 4th to 6th thoracic sympathetic nerves. However, little is known about the contribution of the interactions between sympathetic nerves and breast cancer cells to the malignant progression of TNBC. Methods: The expression levels of the β2-adrenergic receptor (β2-AR, encoded by ADRB2 gene), nerve growth factor (NGF), and tropomyosin receptor kinase A (TrkA) were determined using immunohistochemistry (IHC). NGF expression levels in the serum were compared by enzyme-linked immunosorbent assay (ELISA). Cell proliferation was assessed using the Cell Counting Kit-8 assay. The β2-AR, NGF, p-ERK, and p-CERB expression levels were determined using western blotting. TNBC cells and neuronal cells of the dorsal root ganglion (DRG) in 2-day-old Sprague Dawley rats were co-cultured. Using norepinephrine (NE), NGF, and β2-AR, NGF/TrkA blocker pretreatments, the axon growth of each group of DRG neuron cells was detected by immunofluorescence analysis. Results: The sympathetic adrenergic neurotransmitter NE activated the ERK signaling pathway in TNBC cells. NE/β2-AR signaling promotes NGF secretion. NGF further facilitates the malignant progression of TNBC by increasing sympathetic neurogenesis. In the co-culture assay, the sympathetic adrenergic NE/β2-AR signal pathway also enhanced NGF secretion. NGF binds TrkA in DRG neurons and promotes axonal growth. Conclusion: These results suggest that NE/β2-AR pathway promotes cell proliferation and NGF production in triple-negative breast cancer.

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