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Haksoon Choi,Andreas Ebert,Wen Shi Tsai,Eun Young Yang,Woo Moon Lee,Yun- Chan Huh,Hee Ju Lee,Dong Kum Park,Myeong Cheoul Cho,Bong Nam Jung,Seung Ryong Cheong 한국국제농업개발학회 2012 韓國國際農業開發學會誌 Vol.24 No.5
토마토 TYLCV는 병이 발생되면 약제나 다른 방법으로 방제가 어려우므로 내병성 품종의 개발이 반드시 필요하다. 특히, TYLCV 저항성 유전자 Ty-1,2,3(부분우성)을 한 개체 내로 집적을 시켜서 저항성이 증대된 품종의 개발이 필요하다. 토마토 TYLCV 저항성 11-TY1-AV 등 7계통을 대상으로 보독인 담배가루이를 이용하여 표현형을 조사하였다. 이병성 대비(11-TY5-AV; 슈퍼썬로드)와 F3 세대 3계통 및 F2 세대 4계통을 TYLCTHV 균주를 이용하여 접종하였다. 접종 13일 후에 이병성 대비가 100% 이병증상을 나타내었으며, 이때 11-TY4-AV는 48개체 중에서 23개체가 이병되었으며, 11-TY6-AV는 42개체 중에서 14개체, 11-TY7-AV는 48개체 중에서 14개체, 11-TY8-AV는 48개체 중에서 15개체가 이병 되었다. F2 세대 4 집단 모두 p>0.05 수준에서 TYLCV 접종 후 표현형이 정상적인 유전분리비(3:1)를 보이는 것을 확인 하였다. 또, TYLCV에 저항성인 동일한 자원을 부계로 사용하더라도 모계로 어떤 것을 사용 하느냐에 따라서 저항성 정도가 다르게 나타나는 것을 확인할 수 있었다. Tomato TYLCV is difficult to prevent by chemicals or other methods Thus, developmentof resistant cultivar is certainly needed. Especially, a resistant cultivar is required that show increased resistance through the accumulation of individual TYLCV resistance gene, Ty-1,2 and 3 (partial dominance). The phenotype of resistance was surveyed by the infection of whiteflies against 7 lines such as TYLCV resistant line (11-TY1-AV), susceptibility-control cultivar (11-TY5-AV), 3 lines of F3-generation and 4 lines of F2-generation. These cultivar and lines were artificially inoculated by TYLCTHV strain. The susceptible symptom could be confirmed at one week after the inoculation. After 10 days of inoculation, 83% of susceptible control showed the infected symptom. After 13 days, 100% of the control showed the TYLCV symptom. However, 16 individuals was infected out of 44 individuals in 11-TY4-AV and 12 individuals was infected out of 38 individuals in 11-TY6-AV. Eight individuals was infected out of 43 individuals in 1-TY7-AV, and 11 individuals was infected out of 42 individuals in 11-TY8-AV. Regarding these results, the genetic segregation ratio (3 : 1) in phenotype of 4 lines of F2 generation is following the Mendelism in all lines. Also, it could be confirmed that the resistance level is depending on maternal lines even with same paternal line that is resistant to TYLCV.
Epidemiology of tomato begomoviruses in Taiwan
Chi-Wei Tsai,Wen-Shi Tsai 한국응용곤충학회 2018 한국응용곤충학회 학술대회논문집 Vol.2018 No.10
According to field surveys between 1998 and 2009, four tomatoinfecting begomovirus species detected in Taiwan were Ageratum yellow vein Hualien virus (AYVHuV), Tomato leaf curl Taiwan virus (ToLCTWV), Tomato yellow leaf curl Thailand virus (TYLCTHV), and Tomato leaf curl Hsinchu virus (ToLCHsV). After TYLCTHV first detected in 2005, it quickly replaced ToLCTWV in the tomato fields in Taiwan. In 2009, 8% of the positive samples were infected with ToLCTWV, 51% with TYLCTHV and 41% with mixed infection of both viruses. We performed field surveys again in 2015, 2016, and 2018, and we discovered that all symptomatic tomato plants were infected with ToLCTWV and/or TYLCTHV. Altogether, 59% of the positive samples were infected with ToLCTWV, 33% with TYLCTHV and 8% with mixed infection of both viruses. Surprisingly, the positive samples were more infected with ToLCTWV and less infected with TYLCTHV. To confirm the “revenge” of ToLCTWV in tomato fields, we plan to carry out a more extensive field survey in the upcoming year.
The seasonal detection of AcSBV (Apis cerana sacbrood virus) prevalence in Taiwan
Yu-Shin Nai,Chong-Yu Ko,Pei-Shou Hsu,Wen-Shi Tsai,Yue-Wen Chen,Meng-Hao Hsu,I-Hsin Sung 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1
The epizootic disease caused by Apis cerana sacbrood virus (AcSBV) occurred in Eastern hive bee, A. cerana, since2015 in Taiwan. A large-scale survey of this disease from September and December 2016 in Taiwan was performedincluding symptom check and molecular identification in honey bees of A. cerana hives and several A. mellifera hives, which were co-cultured with A. cerana. Based on the nucleotide sequences of partial VP1, thephylogenetic analysis with those of the known AcSBV isolates revealed that most of AcSBV isolates from Taiwanwere closely relative to SBV-FZ and -JL isolates from China, whereas only one sample (N15-5-1) was in a distinctcluster, which was closely relative to SBV-LN from China too. The AcSBV prevalence was occurring in A. ceranahives in most areas of Taiwan except for those in Hualien and Pingtung Counties in Taiwan. Notably, the AcSBVprevalence rate showed the temporal increase from 47.1% to 69.6% within 4 months. In addition, 37.5% ofAcSBV prevalence rate was found in A. mellifera hives. It showed that A. mellifera was also susceptible to AcSBVinfection. The present results would provide the information on the epidemiology and for prospective research.