The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression

Yan Bin,Li Xurui,Peng Mou,Zuo Yali,Wang Yinhuai,Liu Pian,Ren Weigang,Jinxin Liu 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Aberrant glucose metabolism is a characteristic of bladder cancer. Hyperglycemia contributes to the development and progression of bladder cancer. However, the underlying mechanism by which hyperglycemia promotes the aggressiveness of cancers, especially bladder cancer, is still incompletely understood. N6-methyladenosine (m6A) modification is a kind of methylation modification occurring at the N6 position of adenosine that is important for the pathogenesis of urological tumors. Recently, it was found that the m6A reader YTHDC1 is regulated by high-glucose conditions. In our study, we revealed that YTHDC1 is not only regulated by high-glucose conditions but is also downregulated in bladder cancer tissue and associated with the prognosis of cancer. We also showed that YTHDC1 suppresses the malignant progression of and the glycolytic process in bladder cancer cells in an m6A-dependent manner and determined that this effect is partially mediated by GLUT3. Moreover, GLUT3 was found to destabilize YTHDC1 by upregulating RNF183 expression. In summary, we identified a novel YTHDC1/GLUT3/RNF183 feedback loop that regulates disease progression and glucose metabolism in bladder cancer. Collectively, this study provides new insight regarding the pathogenesis of bladder cancer under hyperglycemic conditions and might reveal ideal candidates for the development of drugs for bladder cancer.

Finite Element Simulation of the Tube Preforming and Hydroforming Process for an Automobile Subframe

Bing Yang,Shuhui. Li,Peng Hu,Zhongqin. Lin,Weigang Zhang 한국소성가공학회 2005 한국소성가공학회 컨퍼런스 Vol.2005 No.11

Optimization Design of Stainless Steel Stamping Multistage Pump Based on Orthogonal Test

Weidong, Shi,Chuan, Wang,Weigang, Lu,Ling, Zhou,Li, Zhang Korean Society for Fluid machinery 2010 International journal of fluid machinery and syste Vol.3 No.4

Stainless steel stamping multistage pump has become the mainstream of civil multi-stage pump. Combined with the technological features of stamping and welding pump, the studies of design for hydraulic parts of pump were come out. An $L_{18}$($3^7$)orthogonal experiment was designed with seven factors and three values including blade inlet angle, impeller outer diameter, guide vane blade number, etc. 18 plans were designed. The two stage of whole flow field on stainless steel stamping multistage pump at design point for design was simulated by CFD. According to the test result and optimization design with experimental research, the trends of main parameters which affect hydraulic performance were got. After being manufactured and tested, the efficiency of the optimal model pump reaches 61.36% and the single head is more than 4.8 m. Compared with the standard efficiency of 53%, the design of the stainless steel stamping pump is successful. The result would be instructive to the design of Stainless steel stamping multistage pump designed by the impeller head maximum approach.

In-situ hydrodeoxygenation of furfural to furans over supported Ni catalysts in aqueous solution

Ze Wang,Zhaolin Fu,Weigang Lin,Songgeng Li,Wenli Song 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.8

In-situ hydrodeoxygenation of furfural as a representative component in bio-oil was investigated in aqueous solution over supported Ni catalysts, for preparing furans as an antiknock additive. The addition of methanol, ethanol, or isopropanol was found inhibitive to coke formation at 220 oC. When using methanol as the hydrogen donor and coke inhibitor, the support in mesoporous structure with moderate acidity was more favorable to the conversion of furfural and to the formation of furans. An increased loading amount of Ni facilitated the generation of deep hydrogenated products. The conversion of furfural could hardly be changed under different methanol to water ratios, while the product distribution varied remarkably. Under optimized conditions, the summary yield of furan and 2-methylfuran reached to above 85%. On the basis of optimized reaction conditions, the in-situ hydrodeoxygenation of an eight-component synthetic bio-oil was tested, and the results verified the adaptability of the method for conversion of bio-oil.

Design and performance research of a mixed-flow submersible deep well pump

Qihua Zhang,Yuanhui Xu,Li Cao,Weidong Shi,Weigang Lu 한국유체기계학회 2016 International journal of fluid machinery and syste Vol.9 No.3

To meet the demand of higher handling capacity, a mixed-flow submersible deep well pump was designed and tested. The main hydraulic components are made of plastics, which is free of erosion, light-weight, and environment-friendly. To simplify plastic molding process, and to improve productivity, an axial-radial guide vane was proposed. To clarify its effect on the performance, a radial guide vane and a space guide vane are developed as well. By comparison, the efficiency of the pump equipped with the axial-radial guide vane is higher than the radial guide vane and is lower than the space guide vane, and its high efficiency range is wide. The static pressure recovery of the axial guide vane is a bit lower than the space guide vane, but it is much larger than the radial guide vane. Taking the cost and molding complexity into consideration, the axial-radial guide vane is much economic, promoting its popularity for the moderate and high specific speed submersible deep well pumps.

Preparation of stable gelatin/sodium carboxymethylcellulose/sodium lauryl sulfonate microcapsules with ultra-thin capsule wall for electrophoretic displays

Gang Wu,Run-Ying Dai,Weigang Li,Pei-Pei Yin,Hongzheng Chen,Mang Wang 한국물리학회 2011 Current Applied Physics Vol.11 No.3

Elastic and optical transparent microcapsules with ultra-thin and compact capsule wall for electrophoretic display containing tetrachloroethylene as core material were prepared from gelatin (GE), sodium lauryl sulfonate (SLS) and sodium carboxymethylcellulose (NaCMC) by complex coacervation method. Microcapsules were characterized by scanning electron microscopy, optical microscopy, and thermogravimetric analysis. The pH value influenced formation process was monitored by recording the microstructure of the fragment of the capsules formed at different pH value. Products with branch-like structure formed with the decrease of pH value, by which the capsule wall was knitted up to form compact structure. SLS was found to play an important role in the course of capsule formation, i.e. SLS helped to increase the interaction between gelatin and NaCMC and facilitate the complex coacervation reaction. In the case of 1.0 mM SLS addition, GE/NaCMC/SLS microcapsules with ultra-thin capsule wall,200 nm in thickness, can be prepared. The capsule owns high thermal stability and good barrier property,both of which prevent core materials from penetrating outside the capsules and are favor of the practical application for the capsules in areas of electrophoretic displays.

Design and performance research of a mixed-flow submersible deep well pump

Zhang, Qihua,Xu, Yuanhui,Cao, Li,Shi, Weidong,Lu, Weigang Korean Society for Fluid machinery 2016 International journal of fluid machinery and syste Vol.9 No.3

To meet the demand of higher handling capacity, a mixed-flow submersible deep well pump was designed and tested. The main hydraulic components are made of plastics, which is free of erosion, light-weight, and environment-friendly. To simplify plastic molding process, and to improve productivity, an axial-radial guide vane was proposed. To clarify its effect on the performance, a radial guide vane and a space guide vane are developed as well. By comparison, the efficiency of the pump equipped with the axial-radial guide vane is higher than the radial guide vane and is lower than the space guide vane, and its high efficiency range is wide. The static pressure recovery of the axial guide vane is a bit lower than the space guide vane, but it is much larger than the radial guide vane. Taking the cost and molding complexity into consideration, the axial-radial guide vane is much economic, promoting its popularity for the moderate and high specific speed submersible deep well pumps.

Preparation and characterization of low density polystyrene/TiO2 core–shell particles for electronic paper application

Xujia Fang,Hong Yang,Gang Wu,Weigang Li,Hongzheng Chen,Mang Wang 한국물리학회 2009 Current Applied Physics Vol.9 No.4

In order to reduce the density mismatch between TiO2 and the low dielectric medium and improve the dispersion stability of the electrophoretic particles in the low dielectric medium for electrophoretic display application, polystyrene/titanium dioxide (PS/TiO2) core–shell particles were prepared via in-situ sol–gel method by depositing TiO2 on the PS particle which was positively charged with 2-(methacryloyloxy) ehyl trimethylammonium chloride (DMC). The morphology and average particle size of PS/TiO2 core–shell particles were observed by transmission electron microscopy (TEM), scanning electron microscope (SEM) and particle size analyzer. It was found that density of PS/TiO2 core–shell particles were reduced obviously and the particles can suspend in the low dielectric medium of low density. The PS/ TiO2 core–shell particles can endure ultrasonic treatment because of the interaction between TiO2 and PS. Zeta potential and electrophoretic mobility of the fabricated core–shell particles in a low dielectric medium with charge control agent was measured to be -44.3 mV and -6.07 × 10-6 ㎠/Vs, respectively, which presents potential in electronic paper application. In order to reduce the density mismatch between TiO2 and the low dielectric medium and improve the dispersion stability of the electrophoretic particles in the low dielectric medium for electrophoretic display application, polystyrene/titanium dioxide (PS/TiO2) core–shell particles were prepared via in-situ sol–gel method by depositing TiO2 on the PS particle which was positively charged with 2-(methacryloyloxy) ehyl trimethylammonium chloride (DMC). The morphology and average particle size of PS/TiO2 core–shell particles were observed by transmission electron microscopy (TEM), scanning electron microscope (SEM) and particle size analyzer. It was found that density of PS/TiO2 core–shell particles were reduced obviously and the particles can suspend in the low dielectric medium of low density. The PS/ TiO2 core–shell particles can endure ultrasonic treatment because of the interaction between TiO2 and PS. Zeta potential and electrophoretic mobility of the fabricated core–shell particles in a low dielectric medium with charge control agent was measured to be -44.3 mV and -6.07 × 10-6 ㎠/Vs, respectively, which presents potential in electronic paper application.