Study on Personalized Course Generation Based on Layered Recommendation Algorithm

Zhihong Wang 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2

The paper introduces the concept of a layered recommendation system (LRS) based on multi-dimensional feature vectors to implement personalized course generation model and algorithms. In this work, we present a personalized course generation algorithm based on the multi-dimensional feature vectors (PCG-LRS) and hybrid applications by content-based recommendations and collaborative filtering recommendation algorithm to generate personalized curriculums. Based on this algorithm, we introduce the teaching outline as the basis of the initial generated course and the final learning goals. The knowledge base of the courses can be constructed from the teaching outline. The initial personalized knowledge models of students are generated by pre-tests. These personalized knowledge models are the base of personalized course generation. This algorithm not only helps teachers to develop the overall curriculum teaching plan and to generate the curriculum automatically, but also meets the learning requirements of each individual student with different knowledge and abilities. Additionally, the layered recommendation algorithm recommends content within a large-scale knowledge base repository and resource base implement at different levels. The personalized recommendation algorithm is divided into a number of steps, which achieves an effective dimensionality reduction, reduces the amount of computation, and improves the courses generated algorithm.

Fixed-Time Coordination Control for Spacecraft Formation Flying via NFFTSM and Sliding Mode Disturbance Observer

Zhihong Wang,Weiduo Hu 제어로봇시스템학회 2023 제어로봇시스템학회 국제학술대회 논문집 Vol.2023 No.10

Network Threat Analysis based on Vulnerability Relation Model

Zhihong Wang 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.1

A vulnerability relative model based on object time Petri net is proposed for threat evaluation. Combined with Object Oriented technology, the definition of network node object and node class is given, which can help attack information modulation design. By defining the threat of an attack and each index’s quantization method, introduce complexity and harmfulness of network attack into the model, and then utilize and improve Dijkstra algorithm to propose non-target oriented and target oriented network threat analysis method.

Analysis of Cellular Tyrosine Phosphorylation via Chemical Rescue of Conditionally Active Abl Kinase

Wang, Zhihong,Kim, Min-Sik,Martinez-Ferrando, Isabel,Koleske, Anthony J.,Pandey, Akhilesh,Cole, Philip A. American Chemical Society 2018 Biochemistry Vol.57 No.8

<P>Identifying direct substrates targeted by protein kinases is important in understanding cellular physiology and intracellular signal transduction. Mass spectrometry-based quantitative proteomics provides a powerful tool for comprehensively characterizing the downstream substrates of protein kinases. This approach is efficiently applied to receptor kinases that can be precisely, directly, and rapidly activated by some agent, such as a growth factor. However, nonreceptor tyrosine kinase Abl lacks the experimental advantage of extracellular growth factors as immediate and direct stimuli. To circumvent this limitation, we combine a chemical rescue approach with quantitative phosphoproteomics to identify targets of Abl and their phosphorylation sites with enhanced temporal resolution. Both known and novel putative substrates are identified, presenting opportunities for studying unanticipated functions of Abl under physiological and pathological conditions.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/2018/bichaw.2018.57.issue-8/acs.biochem.7b01158/production/images/medium/bi-2017-01158c_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/bi7b01158'>ACS Electronic Supporting Info</A></P>

Expression and tissue distribution analysis of vimentin and transthyretin proteins associated with coat colors in sheep (Ovis aries)

Yin Zhihong,Ma Zhisheng,Wang Siting,Hao Shitong,Liu Xinyou,Pang Quanhai,Wang Xinzhuang 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.9

Objective: Pigment production and distribution are controlled through multiple proteins, resulting in different coat color phenotypes of sheep. Methods: The expression distribution of vimentin (VIM) and transthyretin (TTR) in white and black sheep skins was detected by liquid chromatography–electrospray ionization tandem MS (LC–ESI–MS/MS), gene ontology (GO) statistics, immunohistochemistry, Western blot, and quantitative real time polymerase chain reaction (qRT-PCR) to evaluate their role in the coat color formation of sheep. Results: LC–ESI–MS/MS results showed VIM and TTR proteins in white and black skin tissues of sheep. Meanwhile, GO functional annotation analysis suggested that VIM and TTR proteins were mainly concentrated in cellular components and biological process, respectively. Further research confirmed that VIM and TTR proteins were expressed at significantly higher levels in black sheep skins than in white sheep skins by Western blot, respectively. Immunohistochemistry notably detected VIM and TTR in hair follicle, dermal papilla, and outer root sheath of white and black sheep skins. qRT-PCR results also revealed that the expression of VIM and TTR mRNAs was higher in black sheep skins than in white sheep skins. Conclusion: The expression of VIM and TTR were higher in black sheep skins than in white sheep skins and the transcription and translation were unanimous in this study. VIM and TTR proteins were expressed in hair follicles of white and black sheep skins. These results suggested that VIM and TTR were involved in the coat color formation of sheep.

Self-adjusting strategy based on rotating injection for sensorless control of high-power PMSM drives

Xiaofan Wang,Xiaochun Fang,Zhi Wang,Zhihong Zhong,Yizhi Wang,Fei Lin,Zhongping Yang 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

As a sensorless control method suitable for permanent magnet synchronous motor (PMSM) at lowspeed, rotating high-frequency injection method will face some special problems in high power applications. On the one hand, the low switching frequency limits the injected signal frequency, which is very close to the fundamental frequency. On the other hand, under heavy load conditions, the fundamental current amplitude is several hundred times of the negative sequence current. This will result in difficulty in signal extraction. In this paper, current spectrum is analyzed quantitatively based on the parameters of PMSM drive for rail vehicles, which illustrates the limitations of conventional bandpass filters. A method based on self-adjusting filter is proposed. The negative sequence current is successfully extracted, and the precise rotor position is obtained under the condition of variable speed and variable load. Based on a fullscale test platform the effectiveness of the proposed method is proved.

Molecular cloning and expression analysis of the transcription factor CobHLH40 from Cornus officinalis on the basis of de novo transcriptome sequencing

Hou Dianyun,Wang Wenbin,Yang Mengmeng,Li Zhihong,Xu Jingya,Wang Long,Fan Zhenjie,Wang Yaoyao 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.4

Cornus officinalis is a traditional Chinese herbal medicine rich in active substances, such as iridoid glycosides, esters, polysaccharides, and tannins. C. officinalis replenishes the liver and kidney and retracts and removes phlegm. In this study, the candidate transcription factor bHLH40 from C. officinalis (CobHLH40) was predicted to be involved in iridoid glycoside biosynthesis. CobHLH40 was cloned from C. officinalis. Bioinformatics analysis was performed using the Protparatam, the ProtScale, and the SOPMA. CobHLH40 had a cDNA length of 2100 bp and a relative molecular mass of 63.32 kDa, and encoded 573 amino acids. The subcellular localization analysis indicated that CobHLH40 may be located in the nucleus. The comparison of multiple sequence alignments and phylogenetic relationships showed that the CobHLH40 was similar to the MYC2 of other plants, and that CobHLH40 had high homology with the MYC2 of Sesamum indicum. A prokaryotic expression vector was successfully constructed and transformed into the DE3 cells. The induction and expression of the fusion protein of CobHLH40 need further confirmation. The cloning and bioinformatics analysis results of CobHLH40, which may be related to iridoid glycoside synthesis, were screened and predicted for the first time on the basis of C. officinalis transcriptome sequencing. The temporal and spatial expression patterns of C. officinalis were also established. The CobHLH40 prokaryotic expression system provides a basis to further study its function.

Expression of fox-related genes in the skin follicles of Inner Mongolia cashmere goat

Wenjing Han,Xiaoyan Li,Lele Wang,Honghao Wang,Kun Yang,Zhixin Wang,Ruijun Wang,Rui Su,Zhihong Liu,Yanhong Zhao,Yanjun Zhang,Jinquan Li 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: This study investigated the expression of genes in cashmere goats at different periods of their fetal development. Methods: Bioinformatics analysis was used to evaluate data obtained by transcriptome sequencing of fetus skin samples collected from Inner Mongolia cashmere goats on days 45, 55, and 65 of fetal age. Results: We found that FoxN1, FoxE1, and FoxI3 genes of the Fox gene family were probably involved in the growth and development of the follicle and the formation of hair, which is consistent with previous findings. Real-time quantitative polymerase chain reaction detecting system and Western blot analysis were employed to study the relative differentially expressed genes FoxN1, FoxE1, and FoxI3 in the body skin of cashmere goat fetuses and adult individuals. Conclusion: This study provided new fundamental information for further investigation of the genes related to follicle development and exploration of their roles in hair follicle initiation, growth, and development.

A Hybrid Modular Multilevel Converter Topology with an Improved Nearest Level Modulation Method

Wang, Jun,Han, Xu,Ma, Hao,Bai, Zhihong The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1

In this paper, a hybrid modular multilevel converter (MMC) topology with an improved nearest level modulation method is proposed for medium-voltage high-power applications. The arm of the proposed topology contains N series connected half-bridge submodules (HBSMs), one full-bridge submodule (FBSM) and an inductor. By exploiting the FBSM, half-level voltages are obtained in the arm voltages. Therefore, an output voltage with a 2N+1 level number can be generated. Moreover, the total level number of the inserted submodules (SMs) is a constant. Thus, there is no pulse voltage across the arm inductors, and the SM capacitor voltage is rated. With the proposed voltage balancing method, the capacitor voltage of the HBSM is twice the voltage of the FBSM, and each IGBT of the FBSM has a relatively low switching frequency and an equalized conduction loss. The capacitor voltage balancing methods of the two kinds of SMs are implemented independently. As a result, the switching frequency of the HBSM is not increased compared to the conventional MMC. In addition, according to a theoretical calculation of the total harmonic distortion of the electromotive force (EMF), the voltage quality with the presented method can be significantly enhanced when the SM number is relatively small. Simulation and experimental results obtained with a MMC-based inverter verify the validity of the developed method.

The effects of a short sequence enhancer (50-GTGAAATAAATGCAAATAAAGT) and its derived sequences on green fluorescent protein expression

Zhihong Ma,Zhanjun Lv,Xiu-Fang Wang,Xiangyang Jing,Jianjun Cheng 한국유전학회 2014 Genes & Genomics Vol.36 No.4

Cis-regulatory elements are regions of DNAthat regulate the expression of genes located on that samemolecule of DNA. Though these elements are important forgene expression regulation, the functions of cis elementsremain largely unknown. To explore the mechanisms ofgene activation by short sequence enhancers, we examinedthe enhancer activity of short sequence DNA and itsderived sequences using a GFP expression system. Wefound that AA sequence (50-GTGAAATAAATGCAAATAAAGT) induced strong GFP gene expression, while7pieA (50-GTGAAAAAAATGCAAAAAAAGT) did not. We mutated the five T bases of the AA sequence to A, C orG. Our findings indicated that sequences retaining the 7thand/or 17th Ts possessed strong enhancer activity. RT-PCRand RNA synthesis inhibition analysis using actinomycin Drevealed that the enhanced GFP gene expression inducedby the AA sequence occurred at the transcriptional level. To determine whether the AA sequence formed a secondarystructure via atypical complementation, PAGE methodwas used, and the results showed that the AA sequenceformed a secondary structure. Our results support previousevidence that AATAAA is an important composition of ciselements (enhancer/promoter), and suggest that the formationof an unstable stem-loop structure via atypicalcomplementation may be a new mechanism of enhanceractivity.