Comparative analysis of bovine maternal corpus luteum microRNAs with aberrant and normal developed cloned fetus at late gestation

Xiaohu Su,Shenyuan Wang,Guangqi Gao,Xinyu Zho,Lidong Han,Guanghua Su,Jiaqi Zhang,Wanfu Bai,Xiuying Wang,Guangpeng Li,Li Zhang 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background The development efficiency of cloned cattle is extremely low (< 5%), most of them were aborted at late gestation. Based on our previous studies, some recipient cows with a cloned fetus would present as engorged uterine vessels and enlarged umbilical vessels randomly. Abortion involves both maternal and fetal factors. Objective Our aim was to explore this phenomenon by microRNAs expression profile analysis of maternal corpus luteum (CL), which was related to pregnancy maintenance. Methods The present study provided the comparison of maternal CL miRNAs expression of abnormally and normally developed cloned bovine fetus at late gestation (~ 210 days) using RNA-Seq technology. Results We selected two abnormally pregnant cows (abnormal group, AG) and three normally pregnant cows (normal group, NG) and acquired valid reads of 9317,261–12,327,185 (~ 84.53–91.28%) from five libraries. In total, we identified 981 conserved miRNAs and 223 novel miRNAs. 1052 miRNAs were co-expressed, 124 miRNAs were uniquely expressed in AG, and 93 miRNAs were uniquely expressed in the NG. Compared with NG, 11 were significantly overexpressed, and 22 were downregulated (p < 0.05) at AG among 1052 co-expressed miRNAs. The differentially expressed miRNAs-targeted genes were further analyzed by Gene Ontology and KEGG pathway analysis. Notably, the steroid biosynthesis pathway was a significantly enriched term (p < 0.01), which may affect the secretion of progesterone. Conclusion Our research suggested that abnormal miRNAs expression of bovine maternal CL may affect the pregnant status at late gestation.

Study of generalized electric spring modeling based on Hamilton’s principle and its stability

Xiaohu Wang,Chaohui Zhao,Xinyuan Chen,Zhun Huang 전력전자학회 2024 JOURNAL OF POWER ELECTRONICS Vol.24 No.5

The proposed generalized electric spring (G-ES) topology effectively reduces the redundancy caused by the parallel structure of multiple electric springs in a microgrid system. A modeling method for the G-ES is urgently needed to accurately determine the G-ES parameters and to evaluate its transient operation characteristics. The correspondence between Hamilton’s principle in mechanics and electricity is introduced, and the feasibility of Hamilton’s principle under the smart load topology is verified. The modeling method of the G-ES under Hamilton’s principle is discussed, followed by the application of repetitive control strategies on the G-ES. The filter parameters are designed using the normalization method and a simplified parameter design using repetitive controllers was applied. This article introduces the impedance analysis method into the generalized electric spring to analyze its stability under weak network conditions. This work also derives the stability judgment criteria for generalized electric springs. Finally, the feasibility of modeling the G-ES based on the Hamilton’s principle was verified using MATLAB and a DSP hardware system. In addition, the consistency between the G-ES and a single intelligent load was verified under the Hamilton’s modeling principle. The G-ES stability and dynamic performance under this modeling method meet industry requirements.

A first principle study on the magnetic properties of Cu2O surfaces

Xiaohu Yu,Xuemei Zhang,Shengguang Wang,Gang Feng 한국물리학회 2015 Current Applied Physics Vol.15 No.11

Spin-polarized density functional theory calculations were performed to investigate the magnetism of bulk and Cu2O surfaces. It is found that bulk Cu2O, Cu/O-terminated Cu2O(111) and (110) surfaces have no magnetic moment, while, the O-terminated Cu2O(100) and polar O-terminated Cu2O(111) surfaces have magnetism. For low index surfaces with cation and anion vacancy, we only found that the Cu vacancy on the Cu2O(110) Cu/O-terminated surface can induce magnetism. For atomic and molecular oxygen adsorption on the low index surfaces, we found that atomic and molecular oxygen adsorption on the Cuterminated Cu2O(110) surface is much stronger than on the Cu/O-terminated Cu2O(111) and Cuterminated Cu2O(100) surfaces. More interesting, O and O2 adsorption on the surface of Cu/O terminated Cu2O(111) and O2 adsorption on the Cu-terminated Cu2O(110) surface can induce weak ferromagnetism. In addition, we analysis origin of Cu2O surfaces with magnetism from density of state, the surface ferromagnetism possibly due to the increased 2p-3d hybridization of surface Cu and O atoms. This is radically different from other systems previously known to exhibit point defect ferromagnetism, warranting a closer look at the phenomenon.

A positive feedback loop of heparanase/ syndecan1/nerve growth factor regulates cancer pain progression

Xiaohu Su,Bingwu Wang,Zhaoyun Zhou,Zixian Li,Song Tong,Simin Chen,Nan Zhang,Su Liu,Maoyin Zhang 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.1

Background: The purpose of this research was to assess the role of heparanase (HPSE)/syndecan1 (SDC1)/nerve growth factor (NGF) on cancer pain from melanoma. Methods: The influence of HPSE on the biological function of melanoma cells and cancer pain in a mouse model was evaluated. Immunohistochemical staining was used to analyze HPSE and SDC1. HPSE, NGF, and SDC1 were detected using western blot. Inflammatory factors were detected using ELISA assay. Results: HPSE promoted melanoma cell viability, proliferation, migration, invasion, and tumor growth, as well as cancer pain, while SST0001 treatment reversed the promoting effect of HPSE. HPSE up-regulated NGF, and NGF feedback promoted HPSE. High expression of NGF reversed the inhibitory effect of HPSE down-regulation on melanoma cell phenotype deterioration, including cell viability, proliferation, migration, and invasion. SST0001 down-regulated SDC1 expression. SDC1 reversed the inhibitory effect of SST0001 on cancer pain. Conclusions: The results showed that HPSE promoted melanoma development and cancer pain by interacting with NGF/SDC1. It provides new insights to better understand the role of HPSE in melanoma and also provides a new direction for cancer pain treatment.

AFSO: An Adaptative Frame Size Optimization Mechanism for 802.11 Networks

( Xiaohu Ge ),( Cheng-xiang Wang ),( Yang Yang ),( Lei Shu ),( Chuang Liu ),( Lin Xiang ) 한국인터넷정보학회 2010 KSII Transactions on Internet and Information Syst Vol.4 No.3

In this paper, we analyze the impact of different frame types on self-similarity and burstiness characteristics of the aggregated frame traffic from a real 802.11 wireless local area network. We find that characteristics of aggregated frame traffic are affected by both mean frame size and the proportion of specified frame types. Based on this new knowledge, an adaptative frame size optimization (AFSO) mechanism is proposed to improve the transmission efficiency by adaptively adjusting data frame size according to the proportions of different frame types. Simulation results show that our proposed mechanism can effectively regulate the burstiness of aggregated frame traffic and improve the successful delivery rate of data frames when a fixed throughput target is set for 802.11 wireless networks.

Research on Fixture Hole Drilling Quality of Printed Circuit Board

Xiaohu Zheng,Dapeng Dong,Lixin Huang,Qinglong An,Xibin Wang,Ming Chen 한국정밀공학회 2013 International Journal of Precision Engineering and Vol. No.

Printed circuit board (PCB) fixture hole is used for installing PCB board or fixing PCB board during drilling process. The drilling quality parameters involve burr size, hole wall roughness et al. In order to improve drilling quality, proper drilling parameters and tool geometries are important especially the tool geometries. In this study, the drilling machinability of PCB fixture hole is studied,effects of drilling parameters on thrust force, drilling temperature and quality are discussed. The experimental results show that resin will be melted during the drilling process. The increase in spindle speed will lead to drilling temperature rise. The fuzzing and spalling of glass fibers result in the generation of hole wall roughness. The exit burrs and enter burrs are generated from drilling copper foils which located in the upper and lower surfaces. The heights of exit burrs are increased with the elevated feed. Drilling experiments are carried out with 9 drill bits which have different point angles, helix angles and length of chisel edges. Analysis of variance (ANOVA) is carried out for hole quality parameters. Desirability function method is used in multiple response optimization to obtain the optimal tool geometry parameters.

Altered Activity and Functional Connectivity of Superior Temporal Gyri in Anxiety Disorders: A Functional Magnetic Resonance Imaging Study

Xiaohu Zhao,Qian Xi,Peijun Wang,Chunbo Li,Hongjian He 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.4

Objective: The prior functional MRI studies have demonstrated significantly abnormal activity in the bilateral superior temporal gyrus (STG) of anxiety patients. The purpose of the current investigation was to determine whether the abnormal activity in these regions was related to a loss of functional connectivity between these regions. Materials and Methods: Ten healthy controls and 10 anxiety patients underwent noninvasive fMRI while actively listening to emotionally neutral words alternated by silence (Task 1) or threat-related words (Task 2). The participants were instructed to silently make a judgment of each word’s valence (i.e., unpleasant, pleasant, or neutral). A coherence analysis was applied to the functional MRI data to examine the functional connectivity between the left and the right STG, which was selected as the primary region of interest on the basis of our prior results. Results: The data demonstrated that the anxiety patients exhibited significantly increased activation in the bilateral STG than the normal controls. The functional connectivity analysis indicated that the patient group showed significantly decreased degree of connectivity between the bilateral STG during processing Task 2 compared to Task 1 (t = 2.588, p = 0.029). In addition, a significantly decreased connectivity was also observed in the patient group compared to the control group during processing Task 2 (t = 2.810, p = 0.012). Conclusion: Anxiety patients may exhibit increased activity of the STG but decreased functional connectivity between the left and right STG, which may reflect the underlying neural abnormality of anxiety disorder, and this will provide new insights into this disease.

ALIGNMENTS OF GROUP GALAXIES WITH NEIGHBORING GROUPS

Wang, Yougang,Park, Changbom,Yang, Xiaohu,Choi, Yun-Young,Chen, Xuelei IOP Publishing 2009 The Astrophysical journal Vol.703 No.1

<P>Using a sample of galaxy groups found in the Sloan Digital Sky Survey Data Release 4, we measure the following four types of alignment signals: (1) the alignment between the distributions of the satellites of each group relative to the direction of the nearest neighbor group (NNG); (2) the alignment between the major axis direction of the central galaxy of the host group (HG) and the direction of the NNG; (3) the alignment between the major axes of the central galaxies of the HG and the NNG; and (4) the alignment between the major axes of the satellites of the HG and the direction of the NNG. We find strong signal of alignment between the satellite distribution and the orientation of central galaxy relative to the direction of the NNG, even when the NNG is located beyond 3r(vir) of the host group. The major axis of the central galaxy of the HG is aligned with the direction of the NNG. The alignment signals are more prominent for groups that are more massive and with early-type central galaxies. We also find that there is a preference for the two major axes of the central galaxies of the HG and NNG to be parallel for the system with both early central galaxies, however, not for the systems with both late-type central galaxies. For the orientation of satellite galaxies, we do not find any significant alignment signals relative to the direction of the NNG. From these four types of alignment measurements, we conclude that the large-scale environment traced by the nearby group affects primarily the shape of the host dark matter halo, and hence also affects the distribution of satellite galaxies and the orientation of central galaxies. In addition, the NNG directly affects the distribution of the satellite galaxies by inducing asymmetric alignment signals, and the NNG at very small separation may also contribute a second-order impact on the orientation of the central galaxy in the HG.</P>

On the role of surface diffusion in determining the shape or morphology of noble-metal nanocrystals

Xia, Xiaohu,Xie, Shuifen,Liu, Maochang,Peng, Hsin-Chieh,Lu, Ning,Wang, Jinguo,Kim, Moon J.,Xia, Younan National Academy of Sciences 2013 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.110 No.17

<P>Controlling the shape or morphology of metal nanocrystals is central to the realization of their many applications in catalysis, plasmonics, and electronics. In one of the approaches, the metal nanocrystals are grown from seeds of certain crystallinity through the addition of atomic species. In this case, manipulating the rates at which the atomic species are added onto different crystallographic planes of a seed has been actively explored to control the growth pattern of a seed and thereby the shape or morphology taken by the final product. Upon deposition, however, the adsorbed atoms (adatoms) may not stay at the same sites where the depositions occur. Instead, they can migrate to other sites on the seed owing to the involvement of surface diffusion, and this could lead to unexpected deviations from a desired growth pathway. Herein, we demonstrated that the growth pathway of a seed is indeed determined by the ratio between the rates for atom deposition and surface diffusion. Our result suggests that surface diffusion needs to be taken into account when controlling the shape or morphology of metal nanocrystals.</P>

Assessment of the relative tectonic activity of the Longxian–Baoji Fault Zone in the northeastern Tibetan Plateau based on geomorphic indices

Qi Huang,Xiaohu Zhou,Jiyuan You,Shuaishuai Xu,Lushan Liu,Yang Wang 한국지질과학협의회 2023 Geosciences Journal Vol.27 No.6

Since the Late Cenozoic, tectonic deformation has been intense in the Longxian–Baoji Fault Zone (LBFZ), which is at the intersection of the southwest margin of the Ordos Block, the northeast margin of the Tibet Plateau, and the Qinling Orogenic Belt. To evaluate the relative tectonic activity within the LBFZ and discuss the influence of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ, we extracted data of the Qianhe, Hengshuihe, and Jinlinghe River Basins from the ASTER GDEM and analyzed the geomorphic indices, including the hypsometric integral (HI), standardized stream length-gradient index (SL/K) and Hack profile, elongation ratio (Re), the drainage basin asymmetry factor (AF) and valley floor width-to-height ratio (VF). Through geomorphic indices and field investigations, we found that the LBFZ has experienced relatively high tectonic activity. Combined with the index of relative active tectonics (IAT), we compared the tectonic activity strengths of the four major faults in the study area. Among them, the tectonic activities of the Longxian-Qishan Fault (LQF) and the Taoyuan-Guichuansi Fault (TGF) are the highest. The morphology and AF values of the drainage basin of the southwest side of the study area indicate the influence of northwest-southeast compressive stress. The northeastward expansion of the Tibetan Plateau affected the LBFZ region, and the stress brought about by it controlled the tectonic deformation in the region and sculpted the modern landscape. This study is of great significance for understanding the impact of the northeastward expansion of the Tibetan Plateau on the geomorphological evolution of the LBFZ.