중국 이족(彝族) 여성 두식(頭飾)의 형성요인과 특징에 관한 연구

왕혜원(Huiyuan Wang),소황옥(Hwangoak Soh) 한국복식학회 2017 服飾 Vol.67 No.3

A facile method to synthesize magnetic nanoparticles chelated with Copper(II) for selective adsorption of bovine hemoglobin

Huiyuan Guan,Jundong Wang,Siyuan Tan,Qiang Han,Qionglin Liang,Mingyu Ding 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.6

A novel and uncomplicated synthesis method of Cu2+-chelating with carboxyl groups that directly-modified NiFe2O4 magnetic microspheres (NiFe2O4-PAA-Cu2+) was fabricated for selective enrichment and separation of bovine hemoglobin (BHb). First, a carboxyl group directly-modified on NiFe2O4 magnetic microspheres was gained through a facile one-pot solvothermal method. Second, Cu2+ from CuSO4 was brought into use to react with carboxyl groups under mechanical stirring at room temperature. The resulting magnetic microspheres were characterized by distinct instruments that included transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM) to examine the size, morphology, composition and magnetization characterization. The results indicated that the NiFe2O4-PAA-Cu2+ microspheres exhibited good saturation magnetization (36.686 emu g1), which can facilitate magnetic separation under the help of an outside magnetic field. Also, good dispersion and high adsorption ability to BHb (783.53mg g1) can be applied to selective enrichment for bovine hemoglobin and used for selective sorption of BHb protein in bovine blood samples.

Transcriptome Profile Analysis of Cadmium Tolerance in Chinese Flowering Cabbage

Jiewei Wang,Huiyuan Li,Dandan Zou,Jinfeng Zhao,Lianxue Fan,Tao Wu 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.1

Chinese flowering cabbage (Brassica parachinensis L.) is an important leafy vegetable crop that can accumulatehigh levels of cadmium (Cd) and can thus be easily contaminated by this heavy metal. In this study, we performedgenome-wide transcriptional profiling of Cd tolerance in Cd-tolerant cultivar ‘LB70’ using Solexa sequencing to identifygenes and pathways involved in Cd tolerance in Chinese flowering cabbage. Profiling analysis revealed numerous changesin gene expression in response to Cd treatment, including 1669 genes that were downregulated and 1404 genes that wereupregulated. Gene ontology analysis showed that these differentially expressed genes were mainly involved in binding,catalytic processes, metabolism, cellular processes, single-organism processes, biological regulation, localization, andstimulus responses. Several known important Cd tolerance-related genes, such as HMA3, HMA4, and Nramp1, werealso identified. Pathway analysis suggested that two potential novel pathways, namely, the viral carcinogenesis andParkinson’s disease pathways, might be involved in Cd tolerance in Chinese flowering cabbage. The genes andpathways identified in this study provide an important basis for future studies on the molecular mechanism of Cdtolerance in Chinese flowering cabbage.

Dynamics of Bacterial Communities of Lamb Meat Packaged in Air and Vacuum Pouch during Chilled Storage

Taojun Wang,Huiyuan Guo,Hao Zhang,Fazheng Ren,Ming Zhang,Shaoyang Ge,Hailing Luo,Liang Zhao 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.2

In this study, the changes in microbial communities of lamb meat packaged in the air (plastic tray, PT) and in a vacuum pouch (VAC) were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) during the storage at 4°C. For the PT lamb, the total viable count (TVC) was 107 CFU/g on Day 5, and the dominated bacteria were Pseudomonas fragi, P. fluorescens, and Acinetobacter spp. For the VAC lamb, the TVC was 107 CFU/g on Day 9, and the dominated bacteria were lactic acid bacteria, including Carnobacterium divergens, C. maltaromaticum, and Lactococcus piscium. One strain of Pseudomonas spp. also appeared in VAC lamb. The relative abundance of Enterobacteriaceae in VAC lamb was higher than that PT lamb, indicating a more important role of Enterobacteriaceae in spoilage for VAC lamb than that of PT lamb. The microbial compositions changed faster in the lamb stored in a PT than that stored in a VAC, and microbial community compositions of the late storage period were largely different from those of the early storage period for both the conditions. The findings of this study may guide improve the lamb hygiene and prolong the shelf life of the lamb.

Genome-wide identification of microRNAs and phased siRNAs in soybean roots under long-term salt stress

Qian Wang,Yingxia Yang,Guoqing Lu,Xianjun Sun,Youren Feng,Shuangyong Yan,Huiyuan Zhang,Qiyan Jiang,Hui Zhang,Zheng Hu,Rui Chen 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Salinity stress, as the key limiting factor for agricultural productivity, can activate a series of molecular responses and alter gene expression in plants. Endogenous regulatory small RNAs, such as microRNAs (miRNAs) and phased siRNAs (phasiRNAs), play crucial roles during stress adaptation and prevent the injury from environmental circumstances. Objective To identify long-term salt stress responsive miRNAs and phasiRNAs as well as their associated genes and pathways in soybean roots. Methods Small RNA and degradome sequencing strategies were applied to genome widely investigate miRNAs and phasiRNAs in soybean roots under control and long-term salt stress conditions. Results In this study, stringent bioinformatic analysis led to the identifcation of 253 conserved and 38 novel miRNA candidates. Results of expression profling, target and endogenous target mimics predictions provided valuable clues to their functional roles. Furthermore, 156 genes were identifed to be capable of generating 21 nt and 24 nt phasiRNAs, in which 37 candidates were confrmed by degradome data for miRNA-directed cleavage. Approximately 90% of these phasiRNA loci were protein coding genes. And GO enrichment analysis pointed to “signal transduction” and “ADP binding” entries and refected the functional roles of identifed phasiRNA genes. Conclusion Taken together, our fndings extended the knowledge of salt responsive miRNAs and phasiRNAs in soybean roots, and provided valuable information for a better understanding of the regulatory events caused by small RNAs underlying plant adaptations to long-term salt stress.

THE EFFECT OF ACUTE HYPOXIA ON SOD IN THE HUMAN BODY

Yu Qifu,Wang Huiyuan,Li Minggao,Li Zhangqing,Yu Shonghai 한국항공우주의학협회 1991 항공우주의학회지 Vol.1 No.1

Cell-Penetrating Peptide-Modified PLGA Nanoparticles for Enhanced Nose-to-Brain Macromolecular Delivery

Lu Yan,Huiyuan Wang,Yifan Jiang,Jinhua Liu,Zhao Wang,Yongxin Yang,Shengwu Huang,Yongzhuo Huang 한국고분자학회 2013 Macromolecular Research Vol.21 No.4

Macromolecular drugs become an essential part in neuroprotective treatment. However, the nature of ineffective delivery crossing the blood brain barrier (BBB) renders those macromolecules undruggable for clinical practice. Recently, brain target via intranasal delivery have provided a promising solution to circumventing the BBB. Despite the direct route from nose to brain (i.e. olfactory pathway), there still are big challenges for large compounds like proteins to overcome the multiple delivery barriers such as nasal mucosa penetration, intracellular transport along the olfactory neuron, and diffusion across the heterogeneous brain compartments. Herein presented is an intranasal strategy mediated by cell-penetrating peptide modified poly(lactic-co-glycolic acid) (PLGA) nanoparticles for the delivery of insulin to the brain, a potent therapeutic against Alzheimer’s disease. The results revealed that the cell-penetrating peptide can potentially deliver insulin into brain via the nasal route, showing a total brain delivery efficiency of 6%. It could serve as a potential treatment for neurodegenerative diseases.

Safety Evaluation of Lactobacillus paracasei subsp. paracasei LC-01,a Probiotic Bacterium

Hao Zhang,Yu Wang,Jing Sun,Zirui Guo,Huiyuan Guo,Fazheng Ren 한국미생물학회 2013 The journal of microbiology Vol.51 No.5

The safety of Lactobacillus paracasei subsp. paracasei LC-01was evaluated for its use as a potential probiotic. In our in vitro study, the antibiotic resistance and the ability to produce biogenic amine were determined. The results showed that the strain was sensitive to all tested antibiotics and did not produce biogenic amine except for tyramine. The oral toxicity of this strain was evaluated in Balb/C mice. One hundred mice were divided into 10 groups. Four groups were administered 0, 108, 109, or 1010 CFU/mouse per day dissolved in saline solution respectively, for 28 days. Three groups were injected intraperitoneally with 109 CFU/mouse dissolved in saline solution, and were killed 2, 5, and 10 days after injection. The last 3 groups were injected with the vehicle as controls respectively. The results showed that oral administration of the strain had no adverse effects on mouse body weight and that there was no treatment-associated bacterial translocation. Intraperitoneal administration caused a significant translocation to liver, spleen and kidney. However,this translocation did not cause illness or death throughout the experiment. The results suggest that L. paracasei subsp. paracasei LC-01 is likely to be safe for human consumption.

PVA Hydrogel Functionalization via PET-RAFT Grafting with Glycidyl Methacrylate and Immobilization with 2-Hydroxypropyltrimethyl Ammonium Chloride Chitosan via Ring-Open Reaction

Jinsheng Zhou,Yanming Lin,Lin Ye,Ling Wang,Li Zhou,Huiyuan Hu,Qilong Zhang,Hui Yang,Zhongkuan Luo 한국고분자학회 2019 Macromolecular Research Vol.27 No.11

To solve the biofouling problem of polyvinyl alcohol (PVA) hydrogel as the artificial cornea, glycidyl methacrylate (GMA) and 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) were grafted on the surface of PVA hydrogel via a new method of photoinduced electron transfer–reversible addition fragmentation chain transfer (PET-RAFT) polymerization and ring-open reaction. Both attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), scanning electron microscope (SEM), and thermogravimetric analysis (TGA) confirmed that GMA and HACC were successfully grafted on the surface of PVA hydrogel. A series of experiments to test the hydrophilicity of PVA hydrogel showed that it became hydrophobic due to the introduction of hydrophobic groups after grafting with GMA and HACC. In addition, cytotoxicity in vitro of PVA-g-p(GMA-HACC) hydrogel could be considered as not cytotoxicity according to ISO 10993-5: 2009. The anti-fouling property of hydrogel decreased after grafting with GMA due to the hydrophobic surface, while increased after grafting with HACC due to the steric repulsion of p(GMA-HACC) polymer brush. It’s no doubt that PET-RAFT was a feasible and reliable surface modification method which could be used in many biomolecules due to the excellent advantages.