Total Heart Valve Replacement Surgeries and a New Hingeless Valve

Wada, Juro 최신의학사 1969 最新醫學 Vol.12 No.1

Degradation of Serotonin <i>N</i>-Acetyltransferase, a Circadian Regulator, by the N-end Rule Pathway

Wadas, Brandon,Borjigin, Jimo,Huang, Zheping,Oh, Jang-Hyun,Hwang, Cheol-Sang,Varshavsky, Alexander American Society for Biochemistry and Molecular Bi 2016 The Journal of biological chemistry Vol.291 No.33

<P>Serotonin N-acetyltransferase (AANAT) converts serotonin to N-acetylserotonin (NAS), a distinct biological regulator and the immediate precursor of melatonin, a circulating hormone that influences circadian processes, including sleep. N-terminal sequences of AANAT enzymes vary among vertebrates. Mechanisms that regulate the levels of AANAT are incompletely understood. Previous findings were consistent with the possibility that AANAT may be controlled through its degradation by the N-end rule pathway. By expressing the rat and human AANATs and their mutants not only in mammalian cells but also in the yeast Saccharomyces cerevisiae, and by taking advantage of yeast genetics, we show here that two complementary forms of rat AANAT are targeted for degradation by two complementary branches of the N-end rule pathway. Specifically, the N-terminally acetylated (Nt-acetylated) Ac-AANAT is destroyed through the recognition of its Nt-acetylated N-terminal Met residue by the Ac/N-end rule pathway, whereas the non-Nt-acetylated AANAT is targeted by the Arg/N-end rule pathway, which recognizes the unacetylated N-terminal Met-Leu sequence of rat AANAT. We also show, by constructing lysine-to-arginine mutants of rat AANAT, that its degradation is mediated by polyubiquitylation of its Lys residue(s). Human AANAT, whose N-terminal sequence differs from that of rodent AANATs, is longer-lived than its rat counterpart and appears to be refractory to degradation by the N-end rule pathway. Together, these and related results indicate both a major involvement of the N-end rule pathway in the control of rodent AANATs and substantial differences in the regulation of rodent and human AANATs that stem from differences in their N-terminal sequences.</P>

Clot Waveform Analysis for Hemostatic Abnormalities

Wada Hideo,Shiraki Katsuya,Matsumoto Takeshi,Shimpo Hideto,Shimaoka Motomu 대한진단검사의학회 2023 Annals of Laboratory Medicine Vol.43 No.6

Clot waveform analysis (CWA) observes changes in transparency in a plasma sample based on clotting tests such as activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). Evidence indicates that not only an abnormal waveform but also peak times and heights in derivative curves of CWA are useful for the evaluation of hemostatic abnormalities. Modified CWA, including the PT with APTT reagent, dilute PT (small amount of tissue factor [TF]-induced clotting factor IX [FIX] activation; sTF/FIXa), and dilute TT, has been proposed to evaluate physiological or pathological hemostasis. We review routine and modified CWA and their clinical applications. In CWA-sTF/FIXa, elevated peak heights indicate hypercoagulability in patients with cancer or thrombosis, whereas prolonged peak times indicate hypocoagulability in several conditions, including clotting factor deficiency and thrombocytopenia. CWA-dilute TT reflects the thrombin burst, whereas clot-fibrinolysis waveform analysis reflects both hemostasis and fibrinolysis. The relevance and usefulness of CWA-APTT and modified CWA should be further investigated in various diseases.

古墳群の把握と理解 -日本の事例を中心に-

( Wada Seigo ) 공주대학교 백제문화연구소 2013 백제문화 Vol.1 No.48

필자는 일본열도 고분시대(3세기 중엽∼6세기 말)의 정치사회사를 연구의 한 테마로 하고 있다. 그 과정에서 열도의 각지에 만들어진 20만기 전후의 고분(전방후원분, 전방후방분, 원분, 방분의 4가지가 기본형)을 어떤 단위, 또는 그룹으로 파악하고 축조상황(성립·지속·이동·소멸이나 동시기의 조합 등)을 어떻게 이해하느냐가 매우 큰 문제가 되고 있다. 여기에서는 이러한 과제에 필자가 임해 온 방법과 작업의 실제 개요를 보고하고자 한다. 고분시대의 시기구분, 계층구분, 지역구분을 명확히 하면서 일정한 나름대로의 해석을 추가하여 논의를 진행하지만, 작업이 아직 완료된 것이 아니기 때문에 마지막에 약간의 과제와 전망을 덧붙였다. 특히, 아직도 논의가 불충분한 고분의 구분과 지역 구분의 문제는 사람(또는 집단)의 구분과 밀접한 관계가 있기 때문에 신중한 검토가 요구된다. 목적의식에 따라 방법론을 연마하며, 고고자료를 열심히 분석하고, 가설을 세우고, 그것을 끊임없이 검증하는 작업이 중요하다고 생각한다. The writer of this article studies political and social history of Gohun Period(middle of the 3rd to late 6th century AD) in Japan. It is important how to categorize numerous tombs(keyhole-shaped tombs, double squareshaped tombs, circular tombs and square-shaped tombs) about two hundreds thousands by appropriate units and groups, and to understand their construction situation; development, maintain, transfer and extinction of the tombs. This study presents the methods and preliminary results of these issues. It is discussed to clarify the period, hierarchy and region, and new interpretation is added. Further issues and prospects are also included in conclusion as the research is still ongoing. It is necessary to consider the classification and regional issues carefully because they reflect the people or community relating to the tombs. In addition, it is very important to analyse archaeological data, frame a hypothesis and verify it challenging to methodologies according to own objectives.

Correlation between Fat Desposit in Eye Muscle and Concentration of Plasma Lipoprotein in Fattened Steer(日文)

Wada.H. 江原大學校 農科大學 農業科學硏究所 1984 강원 농업생명환경연구 Vol.1 No.-

The effect of daily calf stroking frequency during the postnatal period on the establishment of the human-calf relationship

Wada, Satoko,Fukasawa, Michiru,Chiba, Takashi,Shishido, Tetsuro,Tozawa, Akitsu,Ogura, Shin-ichiro Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.10

Objective: Stroking calves during the postnatal period could effectively improve human-calf relationships. The objective of this study is to examine how daily calf stroking frequency during the postnatal period affects the establishment of human-calf relationships. Methods: Six calves were stroked by a trainer for 6 minutes once daily for 5 days after birth (D1). Six calves were stroked by a trainer for 3 minutes twice daily for 5 days after birth (D2). A further four calves were stared at but not stroked as the control group. The overall stroking or staring duration was the same for all groups, at 6 min/d and 30 min over 5 days. The tests for reactions to the stationary trainer in an unfamiliar environment and avoidance distance measurements for an approaching trainer were conducted at 1 month and 3 months after the treatment. Results: Calves in both stroking groups approached significantly closer to the stationary trainer, vocalized less, and looked at the trainer shorter than the control group at 1 month. However, at 3 months, there was no significant difference between the D1 and the control group, whereas the D2 approached significantly closer to the trainer and vocalized less, and looked at the trainer for a shorter time than the control group. For the avoidance distance, the trainer could approach closer to both stroking groups than the control at 1 month, however, there was no difference among groups at 3 months. Conclusion: Our results suggested that the difference in the calf stroking procedure affected the established human-calf relationships, even though the total stroking duration was the same for all stroked calves. It is likely to be more effective to stroke more frequently than intensively when the aim is to establish better human-calf relationships within limited labor time.

Synthesis of Anatase Phase Titanium Dioxide Using High-Power Nd:YAG Laser Focused on Titanium Wire in Water

Wada, Naoyuki,Kim, Chang-Hwan,Yoh, Jack J.,Hamashima, Hideki,Hokamoto, Kazuyuki The Japan Institute of Metals 2012 Materials Transactions Vol.53 No.1

<P>An experimental method to synthesize titanium dioxide (TiO<SUB>2</SUB>) using high-power laser in water was performed. A high-power Nd:YAG pulsed laser was used for the synthesis, with the laser energy fixed at 1 J/pulse. This laser was focused on a titanium wire set in water. This investigation recovered nano-sized anatase phase titanium dioxide with well crystallized structure. Pulsed bubbles generated in the water were confirmed by optical measurement, and their collapse may have induced high pressures and temperatures. The bubbles generated was approximately spherical in shape, with an estimated maximum size of 3.7 mm generated about 200 µs later after focusing the laser. Recovered powders were confirmed as single anatase phase titanium dioxide by XRD analysis. Effects of bubbles on synthesis and crystallization are also suggested.</P>

도일 후 손창섭의 법적 지위—사망 전후 발행 공적 서류를 근거로

WADA Tomomi 한국현대문학회 2023 한국현대문학연구 Vol.- No.70

This article attempts to verify the legal status of Son Chang-seop at Japan. It is commonly believed that he was naturalized in Japan, but no legal documents have been found to prove his naturalization. The only evidence is the testimony of his wife. The purpose of this article was to reveal his nationality based on several official documents issued in Japan before and after his death in 2010. In addition to the official documents, with reviewing his serialized novel “Yumaeng”, the aspects of the life for him and his family at Japan are clarified. Their life is known to have been very frugal. This is because he and his wife lived in a small public housing. However, the Son’s family lived in the apartment in Tokyo 23 wards at first, and then moved to a public housing in their 50s. Considering the special significance of living in a public complex in the 1980s Japan, this cannot be attributed to economic difficulties. At that time, residents of public housing tended to live temporarily to save money on living expenses while aiming for desirable housing. Similarly, for Son’s family, living in a public housing was a passage to a desirable living environment in later life. However, Son himself did not accept his family’s suggestion and died in a public housing. As for the nationality issue, there is no legal evidence that the family’s wishes were fulfilled. In public documents issued before and after his death in 2010, Son was listed only as a foreign spouse of a Japanese. Japanese are removed from family register upon death. However, there is no record that Son was removed from family register. This indicates that he was never a Japanese citizen. Only the person himself can change his nationality, and it is not possible to change it after death. This brings us to the conclusion of our discussion of Son’s nationality.

Multivalent mannose-decorated NIR nanoprobes for targeting pan lymph nodes

Wada, Hideyuki,Hyun, Hoon,Bao, Kai,Lee, Jeong Heon,El Fakhri, Georges,Choi, Yongdoo,Choi, Hak Soo Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.340 No.-

<P><B>Abstract</B></P> <P>Lymphadenectomy is a prerequisite for most malignancies to define the precise staging of cancer, as well as resect the possible metastases completely. While it improves prognosis, lymphadenectomy often causes postoperative edema or bleeding because of unclear surgical margins. In this study, we synthesized near-infrared (NIR) fluorescent nanoprobes with conjugating various mannose moieties on the surface to target macrophages in the lymph node. Armed with these NIR nanoprobes, we demonstrated the feasibility of intraoperative pan lymph nodes (PLN) mapping and real-time optical imaging under the NIR fluorescence imaging system. We found that even single mannose-conjugated ZW800-1 showed specific uptake in lymph nodes within 4 h, and multiple mannose-employed polyrotaxanes highlighted PLN efficiently with low background signals in major organs. This technology can help surgeons perform lymphadenectomy with ease and safety by identifying all regional lymph nodes proficiently after a single intravenous injection of NIR nanoprobes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> NIR fluorescent nanoprobes to target macrophages in the lymph node. </LI> <LI> Renal clearable nanoprobes enhance the SBR of target tissue. </LI> <LI> Intraoperative pan lymph nodes mapping is demonstrated. </LI> <LI> Surgeons can perform lymphadenectomy with ease and safety using NIR. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Gas permeation properties of c-plane aligned hexagonal tungsten oxide membranes formed by multi-stage synthesis

Wada Shintaro,Hagio Takeshi,Kunishi Hiroto,Park Jae-Hyeok,Phouthavong Vanpaseuth,Yamada Yuta,Terao Toshihiro,Li Xinling,Nijpanich Supinya,Ichino Ryoichi 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.6

Crystalline microporous membranes are promising tools for gas separation because their molecular dimension pores allow us to accurately sieve molecules by size. Recently, microporous hexagonal WO3 was reported as a new potential membrane material and its membrane was found to enable separation of water from water/acetic acid mixtures. Pore size of hexagonal WO3 seemed to be also suitable for separation of small gasses; however, its gas permeation properties have not been reported. Additionally, densification of membranes by decreasing intercrystalline gaps are extremely important in gas separation. One effective method to densify polycrystalline membranes is to repeat membrane synthesis, namely multi-stage synthesis. Here, we attempt to prepare dense hexagonal WO3 membranes on porous tubular supports by multi-stage synthesis and examined their densification and gas permeation properties. Densification was confirmed by permeation of SF6 (gas molecule larger than pores of hexagonal WO3), and its potential for separating small gasses was considered from single gas permeation of He and He/SF6 permselectivity. The results indicated that the multi-stage synthesis is effective to densify the membranes and He/SF6 permselectivity reached 42.8 for three-stage synthesis under modified conditions, implying hexagonal WO3 is a potential membrane material for small gas separation.