Genome-wide identification and analysis of Subtilisin-like serine protease gene family in banana (Musa accuminta L.) and their expression under abiotic stresses

Purwar Shalini,Chugh Vishal,Singh Poornima,Srivastava Akhilesh Kumar,Singh Akhilesh Kumar,Mishra Akhilesh Chandra,Singh Ashutosh,Singh Chandra Mohan 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.1

Subtilisin-like serine proteases (SBT), a serine proteolytic enzymes play an important role in plant growth function and during different stresses responses. The systematic analysis of the SBT gene family in Musa acuminate (MaSBT) has been done and their responses to abiotic stresses in banana variety cv. G-9 were also analyzed. Total of 67 MaSBT genes were identified and based on phylogeny these were grouped into five districted subgroups. Cis-acting element analysis indicated that almost all MaSBT promoters contain regulatory elementary related to growth and development, hormonal regulation, and stress responses. The gene structure and domain analysis showed a maximum of seventeen exons and four functional domains in MaSBT. The 42 orthologous genes, 07 MaSBT paralogous genes were also identified through synteny analysis. The Ka /Ks study indicated that four MaSBT paralogous gene pairs were tandemly duplicated, while the other three were segmental duplications. Further, the expression pattern via RNA-seq data revealed that MaSBTs exhibited differential expres- sion specifically in response to the abiotic stress of low nitrogen. and also during the flowering time. The MaSBT-1.7 gene was found involved in the response to salt stress and flowering. These findings establish a cornerstone for future research on banana's salt stress mechanism. The study offers valuable insights into SBT encoding genes, shedding light on their roles in growth, development, and abiotic stress responses

Fault Immune Pico-Hydro Powered Base Station of Remote Telecommunication Tower

Vishal Verma,Peeyush Pant,Bhim Singh 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.4

This paper presents the dynamic excitation control of a siphon-turbine coupled pico-hydro powered cage rotor induction generator and load matching for off-grid electricity generation. Through the proposed dual-role of the current-controlled voltage source converter (VSC), acting as static synchronous compensator and load controller, real and reactive power are dynamically controlled in a decoupled manner with a self supported DC-bus. The proposed scheme entails minimal computation for ensuring the rated (set) capacity of real power. The scheme also exhibits fault immunity for protection, thus enabling the effective handling of constant power electrical loads presented by base telecom station towers in remote locations. The performance of the system is evaluated under MATLAB/Simulink and is experimented through a developed hardware prototype. Simulation and experimental results show close conformity and validate the effectiveness of the proposed scheme.

Degradation analysis of PCDTBT:PC71BM organic solar cells-an insight

Vishal Sharma,Vinamrita Singh,Manoj Arora,Swati Arora,R.P. Tandon 한국물리학회 2016 Current Applied Physics Vol.16 No.3

The current density-voltage (J-V) characteristics were measured for poly [N-900-heptadeca-nyl-2,7- carbazole-alt-5,5-(40,70-di-2-thienyl-20,10,30-benzo-thiadiazole)]:[6,6]-phenyl-C71-butyric acid methyl ester (PCDTBT:PC71BM) solar cells and studied for degradation analysis up to 150 h of fabrication. It was observed that the dark J-V characteristics in the degraded cell could be explained using both driftdiffusion (D-D) model and space charge limited current (SCLC) model. The D-D model could explain the J-V characteristics at initial voltage region, while SCLC model fitted well in the later part. By comparing the experimental data with theoretical curves, it was found that the transition from D-D model to SCLC model depends on the span of degradation. The results indicated an increase of traps and decrease of charge carrier mobility which may lead to degradation of organic solar cells. This work gave an insight into the degradation mechanism influencing the performance of organic solar cells.

Single Shot White Light Interference Microscopy for 3D Surface Profilometry Using Single Chip Color Camera

Vishal Srivastava,Mohammad Inam,Ranjeet Kumar,Dalip Singh Mehta 한국광학회 2016 Current Optics and Photonics Vol.20 No.6

We present a single shot low coherence white light Hilbert phase microscopy (WL-HPM) for quantitativephase imaging of Si opto-electronic devices, i.e., Si integrated circuits (Si-ICs) and Si solar cells. Whitelight interferograms were recorded by a color CCD camera and the interferogram is decomposed into thethree colors red, green and blue. Spatial carrier frequency of the WL interferogram was increasedsufficiently by means of introducing a tilt in the interferometer. Hilbert transform fringe analysis was usedto reconstruct the phase map for red, green and blue colors from the single interferogram. 3D step heightmap of Si-ICs and Si solar cells was reconstructed at multiple wavelengths from a single interferogram. Experimental results were compared with Atomic Force Microscopy and they were found to be close toeach other. The present technique is non-contact, full-field and fast for the determination of surfaceroughness variation and morphological features of the objects at multiple wavelengths.

Demographic Trends of Patients with Compressive Myelopathy in a Developing Asian Country

Vishal Kumar,Avinash Kumar,Sarvdeep Singh Dhatt,Raj Bahadur 대한척추외과학회 2016 Asian Spine Journal Vol.10 No.2

Study Design: Prospective case series. Purpose: To analyze the demographic picture of the patients suffering from compression myelopathy due to various spinal problems. Overview of Literature: There is a lack of literature depicting demographic picture of such patients with spinal injuries as most of the articles have shown the epidemiology of spinal cord injuries either managed conservatively or operatively. None have focused on the patients with compressive myelopathy requiring surgeries. Methods: Patients with spinal pathologies with a neurological deficit due to compressive myelopathy requiring surgical decompression of dorsal and thoracolumbar region were studied. The different kinds of etiologies, the demographic profiles involved, the involvement of various regions of spine in each of the etiologies, sex distribution of different etiologies, association of age and sex with the occurrence of paraplegia, and association of thoracolumbar junction (TLJ) involvement by age and sex were studied. This study addressed the dorsal and TLJ till L2 vertebrae surgically treated by anterior transthoracic transpleural approach. Results: With regard to gender, 75% of the females and 67.3% of the males were paraplegic but there was no relationship between gender and the occurrence of paraplegia (p >0.05). There was also no association between TLJ involvement and the age and sex of the patients (p >0.05). Seventy percent of the patients were paraplegic with a mean age of 38.90 years and 30% were paraparetic with a mean age of 43.43 years. Male to female ratio stood at 4.43:1. Conclusions: Traumatic spine in females is increasing. The occurrence of paraplegia and involvement of TLJ is not affected by the age and the sex of the patients. Deep epidemiological understanding of spinal pathologies can lead to a better appreciation of the potential impact of health care management strategies and health policies to prevent and minimize their consequences considering limited worldwide reports on the same.

Fault Immune Pico-Hydro Powered Base Station of Remote Telecommunication Tower

Verma, Vishal,Pant, Peeyush,Singh, Bhim The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.4

This paper presents the dynamic excitation control of a siphon-turbine coupled pico-hydro powered cage rotor induction generator and load matching for off-grid electricity generation. Through the proposed dual-role of the current-controlled voltage source converter (VSC), acting as static synchronous compensator and load controller, real and reactive power are dynamically controlled in a decoupled manner with a self supported DC-bus. The proposed scheme entails minimal computation for ensuring the rated (set) capacity of real power. The scheme also exhibits fault immunity for protection, thus enabling the effective handling of constant power electrical loads presented by base telecom station towers in remote locations. The performance of the system is evaluated under MATLAB/Simulink and is experimented through a developed hardware prototype. Simulation and experimental results show close conformity and validate the effectiveness of the proposed scheme.

Response of Upland Cotton (G.hirsutum L.) Genotypes to Drought Stress Using Drought Tolerance Indices

Chandrakant Singh,Vijay Kumar,Indivar Prasad,Vishal R. Patil,Rajkumar B.K. 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.1

Drought tolerance as such is often not considered to be an independent trait by plant breeders. The objective of this study was to evaluate eight drought tolerance indices, namely stress susceptibility index (SSI), yield stability index (YSI), yield reduction ratio (Yr), yield index (YI), tolerance index (TOL), mean productivity (MP), geometric mean productivity (GMP), and stress tolerance index (STI) in upland cotton (G. hirsutum L.) genotypes. For this purpose, 16 genotypes were sampled during the 2013-2014 growing seasons under both normal and drought-stress field conditions at the Main Cotton Research Station of Navsari Agricultural University, Surat, India. The drought tolerance indices were calculated based on seed cotton yield under drought stress and non-stress conditions. Mean comparison of drought tolerance indices and seed cotton yield validated the significant influences of drought stress on yield as well as significant differences among genotypes. Results of calculated correlation coefficients and multivariate analyses showed that GMP, MP and STI indices were able to discriminate drought-sensitive and tolerant genotypes. Cluster analysis using the drought-tolerance indices divided the 16 genotypes into tolerant and susceptible groups. Two genotypes, G.Cot.16 × H-1353/10 and H-1353/10 × G.Cot.16 gave good yield response under drought conditions leading to their stability during water stress conditions. Based on multivariate analyses using the indices individually or in combinations, it was possible to identify the most yield-stable genotypes across the environments. Overall, we concluded that GMP, MP and STI indices can be efficiently exploited not only for screening drought tolerance but also to identify superior genotypessuitable for both stress and non-stress field conditions.

Application of GRA for Optimal Machining Parameter Selection in EDM

BrijKishor Singh,Dinesh Kumar Kasdekar,Vishal Parashar 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.10

Electrical discharge machining (EDM) is one of the most extensively used nonconventional material removal process. The Taguchi method has been utilized to determine the optimal EDM conditions in several industrial fields. The method was design to optimize only a single performance characteristic. To remove this limitation, the Grey relational theory has been used to resolve the complicated interrelationship among the multiple performance characteristics. In the present study, we attempt to find the optimal machining conditions under which the Material removal rate(MRR) to be maximize and Tool wear rate(TWR) to be minimize. This paper summarizes the Grey relation theory and Taguchi optimization technique in order to optimize the cutting parameters in EDM for SS304. The Taguchi method was used to determine the relations between the machining and Response parameters. GRA was used to investigate the optimal machining parameters, among which the pulse on-time, pulse off-time are found to be the most desirable. Finally optimal machining conditions are pulse on time (50 μs), pulse off time (35 μs), discharge current (12A), and voltage (50V). Experimentation was planned as per Taguchi’s L9 (33) orthogonal array. Analysis of variance (ANOVA) is applied to identify the level of importance of the machining parameters on the multiple performance characteristics considered. Finally confirmation result was carried out to identify the effectiveness of this proposed method.

EXISTENCE OF SOLUTION TO BESSEL-TYPE BOUNDARY VALUE PROBLEM VIA G-l CYCLIC F-CONTRACTIVE MAPPING WITH GRAPHICAL VERIFICATION

Deepak Singh,Vishal Joshi,Jong Kyu Kim 경남대학교 수학교육과 2018 Nonlinear Functional Analysis and Applications Vol.23 No.2

The purpose of this paper is to establish the existence of solution to Bessel-type boundary value problem. In the sequel some fixed point theorems are proved. It is worth mentioning that our results cannot be concluded from the existing results in the milieu of associated metric spaces. Utilizing an innovative pictorial technique, an example is worked out substantiating the utility of hypothesis of our results. In an another paper, authors recently established some inventive applications of G-metric spaces in science and engineering.

Tool Wear, Chip formation and Workpiece surface issues in CBN Hard Turning: A Review

Manu Dogra,Vishal S Sharma,Anish Sachdeva,Narinder Mohan Suri,Jasminder Singh Dureja4 한국정밀공학회 2010 International Journal of Precision Engineering and Vol. No.

Steel parts that carry critical loads in everything from automotive drive trains and jet engines to industrial bearings and metal-forming machinery are normally produced by a series of processes, including time-consuming and costly grinding and polishing operations. Due to the advent of super-hard materials such as polycrystalline cubic boron nitride (PCBN) cutting tools and improved machine tool designs, hard turning has become an attractive alternative to grinding for steel parts. The potential of hard turning to eliminate the costs associated with additional finishing processes in conventional machining is appealing to industry. The objective of this paper,is to survey the recent research progress in hard turning with CBN tools in regard of tool wear, surface issues and chip formation. A significant pool of CBN turning studies has been surveyed in an attempt to achieve better understanding of tool wear, chip formation, surface finish, white layer formation, micro-hardness variation and residual stress on the basis of varying CBN content, binder, tool edge geometry, cooling methods and cutting parameters. Further important modeling techniques based on finite element, soft computing and other mathematical approaches used in CBN turning are reviewed. In conclusion, a summary of the CBN turning and modeling techniques is outlined and the scope of future work is presented.