Role of folP1 and folP2 Genes in the Action of Sulfamethoxazole and Trimethoprim Against Mycobacteria

( Tian Zhou Liu ),( Bang Xing Wang ),( Jin Tao Guo ),( Yang Zhou ),( Mugweru Julius ),( Moses Njire ),( Yuan Yuan Cao ),( Tian Wu ),( Zhi Yong Liu ),( Chang Wei Wang ),( Yong Xu ),( Tian Yu Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.9

The combination of trimethoprim (TMP) and sulfamethoxazole (SMX) has been shown to be active against Mycobacterium tuberculosis (Mtb) in clinical tuberculosis (TB) treatment. However, the mechanism of action of TMP-SMX against Mtb is still unknown. To unravel this, we have studied the effect of TMP and SMX by deleting the folP2 gene in Mycobacterium smegmatis (Msm), and overexpressing the Mtb and Msm folP1/2 genes in Msm. Knocking out of the folP2 gene in Msm reduced the minimum inhibitory concentration of SMX 8-fold compared with wild type. Overexpression of the folP1 genes from Mtb and Msm increased the MICs by 4- and 2-fold in Msm for SMX and TMP, respectively. We show a strong correlation between the expression of folP1 and folP2 genes and TMP-SMX resistance in mycobacteria. This suggests that a combination of FolP2 inhibitor and SMX could be used for TB treatment with a better outcome.

Experimental Investigation on Mechanical Properties of Hybrid Steel and Polyethylene Fiber-Reinforced No-Slump High-Strength Concrete

Yuan, Tian-Feng,Lee, Jin-Young,Min, Kyung-Hwan,Yoon, Young-Soo Hindawi Limited 2019 International journal of polymer science Vol.2019 No.-

<P>This paper presents experimental investigations on the mechanical properties of no-slump high-strength concrete (NSHSC), such as the compressive and flexural strength. First, to determine the proper NSHSC mixtures, the compressive and flexural strength of three different water-to-binder ratios (w/b) of specimens with and without polyethylene (PE) fiber was tested at test ages. Then, the effect of hybrid combinations of PE fiber and steel fiber (SF) on the compressive strength, flexural strength, flexural toughness, and flexural energy dissipation capacity was experimentally investigated. Furthermore, the various hybrid fiber-reinforced NSHSCs were evaluated, and their synergy was calculated, after deriving the benefits from each of the individual fibers to exhibit a synergetic response. The test results indicate that a w/b of 16.8% with or without fibers had lower strength and flexural strength (toughness) than those of other mixtures (w/b of 16.4% and 17.2%). Specimens with a hybrid of SF and short PE fibers exhibited a higher compressive and flexural strength, flexural toughness, energy dissipation capacity, and fiber synergy in all considered instances.</P>

A Privacy-aware Graph-based Access Control System for the Healthcare Domain

( Yuan Tian ),( Biao Song ),( M. Mehedi. Hassan ),( Eui-nam Huh ) 한국인터넷정보학회 2012 KSII Transactions on Internet and Information Syst Vol.6 No.10

The growing concern for the protection of personal information has made it critical to implement effective technologies for privacy and data management. By observing the limitations of existing approaches, we found that there is an urgent need for a flexible, privacy-aware system that is able to meet the privacy preservation needs at both the role levels and the personal levels. We proposed a conceptual system that considered these two requirements: a graph-based, access control model to safeguard patient privacy. We present a case study of the healthcare field in this paper. While our model was tested in the field of healthcare, it is generic and can be adapted to use in other fields. The proof-of-concept demos were also provided with the aim of valuating the efficacy of our system. In the end, based on the hospital scenarios, we present the experimental results to demonstrate the performance of our system, and we also compared those results to existing privacy-aware systems. As a result, we ensured a high quality of medical care service by preserving patient privacy.

Canonical transient receptor potential channels and their modulators: biology, pharmacology and therapeutic potentials

Yuan-Yuan Gao,Wen Tian,Hui-Nan Zhang,Yang Sun,Jing-Ru Meng,Wei Cao,Xiao-Qiang Li 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.4

Canonical transient receptor potential channels(TRPCs) are nonselective, high calcium permeability cationicchannels. The TRPCs family includes TRPC1, TRPC2,TRPC3, TRPC4, TRPC5, TRPC6, and TRPC7. These channelsare widely expressed in the cardiovascular and nervoussystems and exist in many other human tissues and celltypes, playing several crucial roles in the human physiologicaland pathological processes. Hence, the emergenceof TRPCs modulators can help investigate these channels’applications in health and disease. It is worth noting that theTRPCs subfamilies have structural and functional similarities,which presents a signifi cant diffi culty in screening anddiscovering of TRPCs modulators. In the past few years,only a limited number of selective modulators of TRPCswere detected; thus, additional research on more potent andmore selective TRPCs modulators is needed. The presentreview focuses on the striking desired therapeutic eff ectsof TRPCs modulators, which provides intel on the structuralmodifi cation of TRPCs modulators and further pharmacologicalresearch. Importantly, TRPCs modulators cansignifi cantly facilitate future studies of TRPCs and TRPCsrelated diseases.

OK-432 Suppresses Proliferation and Metastasis by Tumor Associated Macrophages in Bladder Cancer

Tian, Yuan-Feng,Tang, Kun,Guan, Wei,Yang, Tao,Xu, Hua,Zhuang, Qian-Yuan,Ye, Zhang-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

OK-432, a Streptococcus-derived anticancer immunotherapeutic agent, has been applied in clinic for many years and achieved great progress in various cancers. In the present study, we investigated its anticancer effect on bladder cancer through tumor associated macrophages (TAMs). MTS assay validated OK-432 could inhibit proliferation in both T24 and EJ bladder cell lines. OK-432 also induced apoptosis of bladder cancer cells in vitro. Consequently, we demonstrated that OK-432 could suppress the bladder cancer cells migration and invasion by altering the EMT-related factors. Furthermore, using SD rat model, we revealed that OK-432 inhibited tumor growth, suppressed PCNA expression and inhibited metastasis in vivo. Taken together, these findings strongly suggest that OK-432 inhibits cell proliferation and metastasis through inducing macrophages to secret cytokines in bladder cancer.

SF6 abatement in a packed bed plasma reactor: Role of zirconia size and optimization using RSM

Yuan Tian,Xiaoxing Zhang,Yufei Wang,Zhaolun Cui,Ju Tang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.94 No.-

This work describes plasma destruction of SF6 in a zirconia (ZrO2) packed bed plasma reactor (PBR). Electric signals, equivalent parameters, emission spectra and degradation results have been utilized toevaluate the influence of ZrO2 size on PBR discharge characteristics and SF6 degradation. The resultspresent that the size of ZrO2 has a significant impact on PBR discharge characteristics due to its influenceon physical parameters. Moreover, bigger ZrO2 beads packing shows a better performance in SF6degradation because of longer gas residence time. In addition, the key operating parameters includingflowrate, SF6 concentration, oxygen concentration, and water vapor concentration were optimized byresponse surface methodology (RSM) with Box-Behnken design (BBD). The proposed optimization modelshows satisfactory correlation between the predicted and actual results. For energy yield (EY), the mutualeffect offlowrate, SF6 concentration and water vapor concentration was significant. For SO2F2 selectivity,the mutual effect of SF6 concentration and water vapor concentration was significant. The optimum SF6abatement was predicted from RSM as 14.64 g/kWh and 14.42% for EY and SO2F2 selectivity atflowrate of200 mL/min, SF6 concentration of 3%, oxygen concentration of 0.83% and water vapor concentration of1.89%.

State-dependent Impulsive Control for Consensus of Multi-agent Systems

Yuan Tian,Chuandong Li 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.12

This paper aims to investigate the consensus of multi-agent systems, where each agent runs hopfied-type neural networks, by means of the state-dependent impulsive control. The challenge comes from the fact that the occurrence of impulse varies according to the state of the agent, which means it is hard to predict when an impulse occurs. To solve this problem, we try to transform the impulsive system from state-dependent to fixed-time. In order to do that, we first construct a global consensus error state-dependent with a multi-agent system according to the impulsive consensus protocol. Then we use the B-equivalence method to form a comparison impulsive system of fixed-time impulsive sequence. Using Lyapunov stability theory, we prove that these two systems have the same stability. So we establish sufficient consensus conditions of multi-agent system by analyzing the comparison system. We perform extensive evaluations to validate the correctness of theoretical results and the effectiveness of the statedependent impulsive consensus protocol.

Liposome-mediated Induction of Apoptosis of Human Hepatoma Cells by C-Myc Antisense Phosphorothioate Oligodeoxynucleotide and 5-Fluorouracil

Yuan, Yuan,Cai, Hui,Yang, Xiao-Jun,Li, Wei,He, Jin,Guo, Tian-Kang,Chen, Yi-Rong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: The aim of this study was to investigate the effect of a c-myc antisense oligodeoxynucleotide and 5-fluorouracil on the expression of c-myc, invasion and proliferation of HEPG-2 liver cancer cells. Materials and Methods: HEPG-2 cells were treated with lipiosome-mediated c-myc ADSON and 5-fluorouracil. The proliferation inhibition rate and invasion were measured by MTT and invasion assay, respectively. Cell apoptosis was detected by flow cytometry and expression of c-myc by RT-PCR and immunohistochemistry. Results: The proliferation inhibition rate was significantly higher in the antisense oligodeoxynucleotide added-5-fluorouracil group than single antisense oligodeoxynucleotide or 5-fluorouracil group (p<0.05). G0/G1 cells in the antisense oligodeoxynucleotide group and S cells in the 5-fluorouracil groups were significantly increased than that in the control group, respectively (P<0.01). The amplification strips of PCR products in 5-FU, ASODN and combination groups were significantly weaker than that in the control group (P<0.01). The percentage of c-myc-protein-positive cells were significantly lower in antisense oligodeoxynucleotide, 5-fluorouracil and combination groups than that in the control group (P<0.01). Conclusions: A liposome-mediated c-myc antisense oligodeoxynucleotide and 5-fluorouracil can inhibit the proliferation and invasion of liver cancer cells by reducing the expression of c-myc. A c-myc antisense oligodeoxynucleotide can increase the sensitivity of liver cancer cells to 5-fluorouracil and decrease the dosage of the agent necessary for efficacy, providing an experimental basis for the clinical therapy of liver cancer.

Performance Analysis of Detection Threshold on OFDM Time Synchronization Using PN Sequences

Yuan Tian,Xia Lei,Wanbin Tang,Shaoqian Li 보안공학연구지원센터 2008 International Journal of Software Engineering and Vol.2 No.3

Many synchronization algorithms for orthogonal frequency division multiplexing (OFDM) systems have been proposed. Among them, the time synchronization algorithm by correlating the training sequence with the local known sequence can obtain good performance with a sharp synchronization peak. Detection threshold is an important and necessary parameter in time synchronization. In this paper, based on this algorithm, we evaluate three different methods of setting threshold and point out one method which performs well when SNR is in large dynamic range. The simulation results are consonant with our theoretic analyses and show that this method is suitable for OFDM systems in multi-path fading channel.

Identification and characterization of CONSTANS-like genes from Curcuma alismatifolia

Yuan-Yuan Li,Xiao-Huang Chen,Hui-Wen Yu,Qi-Lin Tian,Luan-Mei Lu 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

The CONSTANS ( CO ) and CONSTANS - like ( COL ) genes have an important role in the regulation of fl owering in photoperiod-sensitive plants. However, information on the molecular characterization of COL genes in Curcuma alismatifoliais very limited. The objective of this study was to identify and evaluate 8 COL homologs ( CaCOLs ) in the C. alismatifoliatranscriptome. Their structures, phylogenetic relationships and expressing patterns under short-day (SD) and long-day (LD)contexts were investigated. Our results showed that CaCOLs were classifi ed into three groups: CaCOL3 and CaCOL6 in groupI were the most CO - like genes; CaCOL7 and CaCOL8 in group II contains only one B-box; CaCOL1 to 2 and CaCOL4 to 5formed the group III. Phylogenetic analysis of the CaCOLs in Arabidopsis, Rice, Barley, Malaccensis and C. alismatifoliademonstrated that both the B-box and CCT domains were conserved in dicot and monocot plants, but diff erent groups ofCOL genes evolved independently. Diff erent patterns of mRNA accumulation in C. alismatifolia leaves in response to SDand LD treatments were observed. CaCOL1 , CaCOL2 , CaCOL4 and CaCOL7 showed signifi cantly higher expressions inLD treatment than that of SD, indicating that they were potential fl owering inducers, and are candidate genes for research infl owering regulation and circadian controlling in C. alismatifolia plants. Altogether, our study facilitates successful regulationof fl owering in Curcuma species and provides insights for future molecular breeding programs.