Induction of Apoptosis in Anoikis-Resistant Breast Cancer Stem Cells by Supercritical CO2 Extracts from Citrus hassaku Hort ex Tanaka

Linh Thi Thao Nguyen,송연우,Thao Anh Tran,김기석,김소미 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

Anoikis (or cell-detachment-induced apoptosis) is aspecial form of apoptosis in which cells loose contact with othercells or the extracellular matrix. In the present study, we havefound that the breast cancer stem cell line MCF-7-SC, isolatedfrom MCF-7 breast cancer cells, shows characteristics of anoikisresistance. Treatment of supercritical CO2 extract from Citrushassaku Hort ex Tanaka, locally known as Phalsak, reduced theexpression of ATP-binding cassette sub-family G member 2mRNA and induced apoptosis in MCF-7-SCs, as evidenced by anincrease of apoptotic body formation, increased cell population inthe sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolyticactivation of caspase-9 and caspase-3, and the degradation ofpoly(ADP-ribose) polymerase (PARP) protein. The effects ofsupercritical CO2 extract from Phalsak on anoikis-resistant breastcancer stem cells suggest the use of this extract as a potentialtherapeutic agent for breast cancer stem cell treatment.

Induction of Apoptosis in Anoikis-Resistant Breast Cancer Stem Cells by Supercritical $CO_2$ Extracts from Citrus hassaku Hort ex Tanaka

Nguyen, Linh Thi Thao,Song, Yeon Woo,Tran, Thao Anh,Kim, Ki-Seok,Cho, Somi Kim The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4

Anoikis (or cell-detachment-induced apoptosis) is a special form of apoptosis in which cells loose contact with other cells or the extracellular matrix. In the present study, we have found that the breast cancer stem cell line MCF-7-SC, isolated from MCF-7 breast cancer cells, shows characteristics of anoikis resistance. Treatment of supercritical $CO_2$ extract from Citrus hassaku Hort ex Tanaka, locally known as Phalsak, reduced the expression of ATP-binding cassette sub-family G member 2 mRNA and induced apoptosis in MCF-7-SCs, as evidenced by an increase of apoptotic body formation, increased cell population in the sub-G1 phase, increase in the Bax/Bcl-2 ratio, proteolytic activation of caspase-9 and caspase-3, and the degradation of poly(ADP-ribose) polymerase (PARP) protein. The effects of supercritical $CO_2$ extract from Phalsak on anoikis-resistant breast cancer stem cells suggest the use of this extract as a potential therapeutic agent for breast cancer stem cell treatment.

Erratum Anomalous Left Coronary Artery from the Pulmonary Artery Syndrome: A Case Report in a 49-Year-Old Woman

Thao Tram Le Nguyen,Xuan Loc Vu Doan,Vy Thuc Nguyen 아시아심장혈관영상의학회 2021 Cardiovascular Imaging Asia Vol.5 No.3

In the published article, “Anomalous Left Coronary Artery from the Pulmonary Artery Syndrome: A Case Report in a 49-Year-Old Woman”, the affiliation was published incorrectly. 1Diagnostic Imaging Department, Vinmec Nha Trang International Hospital, Nha Trang, Vietnam The affiliation should be corrected as follows. Changes are marked by underlines: 1Diagnostic Imaging Department, Vinmec Nha Trang International Hospital, Vinmec Healthcare System, Nha Trang, Vietnam

Anti-inflammatory components of the Vietnamese starfish <i>Protoreaster nodosus</i>

Thao, Nguyen Phuong,Luyen, Bui Thi Thuy,Koo, Jung Eun,Kim, Sohyun,Koh, Young Sang,Cuong, Nguyen Xuan,Nam, Nguyen Hoai,Van Kiem, Phan,Kim, Young Ho,Van Minh, Chau BioMed Central 2015 Biological research Vol.48 No.1

<P><B>Background</B></P><P>In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols <B>(1–4)</B> isolated from the Vietnamese starfish <I>Protoreaster nodosus</I> on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA).</P><P><B>Results</B></P><P>The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC<SUB>50</SUB> values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 μg/mL. Four highly pure steroid derivatives <B>(1–4)</B> were isolated from the dichloromethane fraction and water layer of <I>P. nodosus</I>. Potent inhibitory activities were also observed for (25<I>S</I>) 5α-cholestane-3β,4β,6α,7α,8β,15α,16β,26-octol <B>(3)</B> on the production of IL-12 p40 and IL-6 (IC<SUB>50s</SUB> = 3.11 ± 0.08 and 1.35 ± 0.03 μM), and for (25<I>S</I>) 5α-cholestane-3β,6α,8β,15α,16β,26-hexol <B>(1)</B> and (25<I>S</I>) 5α-cholestane-3β,6α,7α,8β,15α,16β,26-heptol <B>(2)</B> on the production of IL-12 p40 (IC<SUB>50s</SUB> = 0.01 ± 0.00 and 1.02 ± 0.01 μM). Moreover, nodososide <B>(4)</B> exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production.</P><P><B>Conclusion</B></P><P>This is the first report of the anti-inflammatory activity from the starfish <I>P. nodosus</I>. The main finding of this study is the identification oxygenated steroid derivatives from <I>P. nodosus</I> with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.</P>

Doxorubicin and paclitaxel co-bound lactosylated albumin nanoparticles having targetability to hepatocellular carcinoma

Thao, Le Quang,Lee, Changkyu,Kim, Bomi,Lee, Sungin,Kim, Tae Hwan,Kim, Jong Oh,Lee, Eun Seong,Oh, Kyung Taek,Choi, Han-Gon,Yoo, Sun Dong,Youn, Yu Seok Elsevier 2017 Colloids and surfaces Biointerfaces Vol.152 No.-

<P><B>Abstract</B></P> <P>Anticancer drug targeting to liver asialoglycoprotein receptors (ASGPR) is viewed as a good approach for hepatocellular carcinoma (HCC) treatment. Lactose residue is a promising ASGPR ligand due to its high receptor affinity. Herein, we introduce doxorubicin and paclitaxel co-bound lactosylated albumin (Lac-BSA) nanoparticles (Dox/Pac Lac-BSA NPs) with good liver targetability. Lac-BSA was synthesized by conjugating lactobionic acid to naïve BSA then characterized by mass spectrometry. Dox/Pac Lac-BSA NPs were fabricated utilizing high-pressure homogenization and evaporation with Nab<SUP>®</SUP> (nanoparticle albumin bound) technology. Dox/Pac Lac-BSA NPs were spherical and well-dispersed, with a 148.7±13.8nm particle size and −54.1±0.7mV zeta potential at a 100% Lac-BSA feed ratio. Combined Dox and Pac synergistic cytotoxicity was confirmed in Hep G2 cells. Specifically, the inhibitory concentration (IC<SUB>50</SUB>; 0.21±0.02μg/ml) for Dox/Pac Lac-BSA NPs was 3.2 time lower than plain Dox/Pac BSA NPs (IC<SUB>50</SUB>; 0.68±0.04μg/ml). Also, Dox/Pac Lac-BSA NPs exhibited better internalizing in Hep G2 cells (61.8% vs. 14.4% for Dox) and spheroids compared to Dox/Pac BSA NPs. Finally, Dox/Pac Lac-BSA NPs displayed much greater localization into ICR mice livers compared to Dox/Pac BSA NPs. This was indicated by the presence of NP lactose residues revealed by a galactose inhibition study. Based on these results, we suggest that lactose-modified albumin-based nanoparticles fabricated with the Nab<SUP>®</SUP> technique can be a potential therapeutic vector for treating HCC via hepatocyte targeting.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

My Life in Korea

Thao Nguyen 대한토목학회 2016 대한토목학회지 Vol.64 No.1

A Survey on Face-based Cryptographic Key Generation

Thao Dang,Deokjai Choi (사)한국스마트미디어학회 2020 스마트미디어저널 Vol.9 No.2

Derivation cryptographic keys from human biometrics opens a new promising research area when it can be used efficiently for not only verification or recognition tasks, but also symmetric-key based applications. Among existing biometric traits, face is considered as the most popular biometrics since facial features are informative and discriminative. In this paper, we present a comprehensive survey of Face-based key generation (FKGS). First, we summarize the trend of FKGS researches and sum up the methods which play important roles in the proposed key generation systems. Then we present the evaluation and the general performance analysis; from that, we give a discussion about the advantages and disadvantages of surveyed studies to clarify the fundamental requirements and the main challenges when implementing FKGS in practice. Finally, an outlook on future prospects is given

Effects of Supplementation of Eucalyptus (E. Camaldulensis) Leaf Meal on Feed Intake and Rumen Fermentation Efficiency in Swamp Buffaloes

Thao, N.T.,Wanapat, M.,Kang, S.,Cherdthong, A. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.7

Four rumen fistulated swamp buffaloes were randomly assigned according to a $4{\times}4$ Latin square design to investigate the effects of Eucalyptus (E. Camaldulensis) leaf meal (ELM) supplementation as a rumen enhancer on feed intake and rumen fermentation characteristics. The dietary treatments were as follows: T1 = 0 g ELM/hd/d; T2 = 40 g ELM/hd/d; T3 = 80 g ELM/hd/d; T4 = 120 g ELM/hd/d, respectively. Experimental animals were kept in individual pens and concentrate was offered at 0.3% BW while rice straw was fed ad libitum. The results revealed that voluntary feed intake and digestion coefficients of nutrients were similar among treatments. Ruminal pH, temperature and blood urea nitrogen concentrations were not affected by ELM supplementation; however, ELM supplementation resulted in lower concentration of ruminal ammonia nitrogen. Total volatile fatty acids, propionate concentration increased with the increasing level of EML (p<0.05) while the proportion of acetate was decreased (p<0.05). Methane production was linearly decreased (p<0.05) with the increasing level of ELM supplementation. Protozoa count and proteolytic bacteria population were reduced (p<0.05) while fungal zoospores and total viable bacteria, amylolytic, cellulolytic bacteria were unchanged. In addition, nitrogen utilization and microbial protein synthesis tended to increase by the dietary treatments. Based on the present findings, it is suggested that ELM could modify the rumen fermentation and is potentially used as a rumen enhancer in methane mitigation and rumen fermentation efficiency.

Comparative and bioinformatics analyses of pathogenic bacterial secretomes identified by mass spectrometry in Burkholderia species

Thao Thi Nguyen,전태수,김재한,서영수,허무영 한국미생물학회 2017 The journal of microbiology Vol.55 No.7

Secreted proteins (secretomes) play crucial roles during bacterial pathogenesis in both plant and human hosts. The identification and characterization of secretomes in the two plant pathogens Burkholderia glumae BGR1 and B. gladioli BSR3, which cause diseases in rice such as seedling blight, panicle blight, and grain rot, are important steps to not only understand the disease-causing mechanisms but also find remedies for the diseases. Here, we identified two datasets of secretomes in B. glumae BGR1 and B. gladioli BSR3, which consist of 118 and 111 proteins, respectively, using mass spectrometry approach and literature curation. Next, we characterized the functional properties, potential secretion pathways and sequence information properties of secretomes of two plant pathogens in a comparative analysis by various computational approaches. The ratio of potential non-classically secreted proteins (NCSPs) to classically secreted proteins (CSPs) in B. glumae BGR1 was greater than that in B. gladioli BSR3. For CSPs, the putative hydrophobic regions (PHRs) which are essential for secretion process of CSPs were screened in detail at their N-terminal sequences using hidden Markov model (HMM) – based method. Total 31 pairs of homologous proteins in two bacterial secretomes were indicated based on the global alignment (identity ≥ 70%). Our results may facilitate the understanding of the species-specific features of secretomes in two plant pathogenic Burkholderia species.

Effects of Eucalyptus Crude Oils Supplementation on Rumen Fermentation, Microorganism and Nutrient Digestibility in Swamp Buffaloes

Thao, N.T.,Wanapat, M.,Cherdthong, A.,Kang, S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.1

This study was conducted to investigate the effects of eucalyptus (E. Camaldulensis) crude oils (EuO) supplementation on voluntary feed intake and rumen fermentation characteristics in swamp buffaloes. Four rumen fistulated swamp buffaloes, body weight (BW) of $420{\pm}15.0$ kg, were randomly assigned according to a $2{\times}2$ factorial arrangement in a $4{\times}4$ Latin square design. The dietary treatments were untreated rice straw (RS) without EuO (T1) and with EuO (T2) supplementation, and 3% urea-treated rice straw (UTRS) without EuO (T3) and with EuO (T4) supplementation. The EuO was supplemented at 2 mL/h/d in respective treatment. Experimental animals were kept in individual pens and concentrate mixture was offered at 3 g/kg BW while roughage was fed ad libitum. Total dry matter and roughage intake, and apparent digestibilites of organic matter and neutral detergent fiber were improved (p<0.01) by UTRS. There was no effect of EuO supplementation on feed intake and nutrient digestibility. Ruminal pH and temperature were not (p>0.05) affected by either roughage sources or EuO supplementation. However, buffaloes fed UTRS had higher ruminal ammonia nitrogen and blood urea nitrogen as compared with RS. Total volatile fatty acid and butyrate proportion were similar among treatments, whereas acetate was decreased and propionate molar proportion was increased by EuO supplementation. Feeding UTRS resulted in lower acetate and higher propionate concentration compared to RS. Moreover, supplementation of EuO reduced methane production especially in UTRS treatment. Protozoa populations were reduced by EuO supplementation while fungi zoospores remained the same. Total, amylolytic and cellulolytic bacterial populations were increased (p<0.01) by UTRS; However, EuO supplementation did not affect viable bacteria. Nitrogen intake and in feces were found higher in buffaloes fed UTRS. A positive nitrogen balance (absorption and retention) was in buffaloes fed UTRS. Supplementation of EuO did not affect nitrogen utilization. Both allantoin excretion and absorption and microbial nitrogen supply were increased by UTRS whereas efficiency of microbial protein synthesis was similar in all treatments. Findings of present study suggested that EuO could be used as a feed additive to modify the rumen fermentation in reducing methane production both in RS and UTRS. Feeding UTRS could improve feed intake and efficiency of rumen fermentation in swamp buffaloes. However, more research is warranted to determine the effect of EuO supplementation in production animals.