생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

Misorientation Characteristics at the Growth Front of Abnormally-Growing Goss Grains in Fe–3%Si Steel

Tae‑Young Kim,Tae‑Wook Na,Hyung‑Seok Shim,Yong‑Keun Ahn,Yong‑Kwon Jeong,Heung Nam Han,Nong‑Moon Hwang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12

Penetrating morphologies at the growth front of abnormally-growing Goss grains in Fe–3%Si steel was examined in view ofthe possibility that they are the two-dimensional section of the solid-state wetting (SSW) along the triple junction line. Toobtain the statistically meaningful data, 102 penetrated grain and 204 penetrating abnormally-growing Goss grain morphologieswere examined. The misorientation angles of penetrating and penetrated grains were measured by electron backscattereddiffraction. Among the 102 examined penetrated grain boundaries, none has low misorientation angles less than 15°,whereas 17.2% of the 204 penetrating grain boundaries have low misorientation angles, and 23.5% of them have coincidencesite lattice boundaries. Besides, boundary energies of penetrating Goss grains, which were estimated from misorientationangles of the three grains in the penetrating morphology, satisfied the energetic condition for SSW along the triple junctionline. These results imply that the abnormal grain growth of Goss grains in Fe–3%Si steel occurs by the mechanism of subboundaryenhanced SSW.

昭陽湖 赤湖 底質土 微生物學的 物質 分解에 관하여

安泰奭 江原大學校 附設 環境硏究所 1991 環境硏究 Vol.8 No.-

For elucidating the ecological functions of sediment and parts of carbon and phosphorus cycle, the degradation rates of organic phosphate, cellulose and cellobiose were estimated in the sediment of red tide area of Lake Soyang. The breakdown rates of cellulose ranged between 11.0~99.0%/month, and the major factor for breakdown was temperature. The range of cellobiose degradation rates was between 0.8~4.7%/hr, high in spring and low in summer. The organic phosphate degradation rates, showing the range of 16.4~40.4%/hr, were high in winter, just after the Peridinium blooming and spring.

파로호 수중생태계에서의 미생물 분포 및 활성도

안태석,이동훈 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

가정자로법에 의한 촉각궤환장치의 최적 설계

안인석,임태균 위덕대학교 부설 전자기술연구소 1999 전자기술연구소 논문집 : 위덕대 Vol.3 No.1

원격조종로봇 시스템은 인간 활동 영역의~확대와 복지 증진에 의해 세계적으로 널리 사용되고 있다. 이 로봇을 인간이 보다 효율적으로 조종하기 위해서는 조종자가 마치 현장에 있는 것과 같은 가상 현실감을 느끼게 하는 것이 중요하다. 그러나 현재까지 조종자에게 촉각정보를 제공하는 촉각궤환장치는 효과적이지 못하다. 이에 본 논문에서는 더욱 정교하고 효과적인 작업을 할 수 있도록 하는 원격조종로봇용 촉각궤환장치를 최적화 기법을 이용하여 설계하고자 하였다. 먼저, 어레이 형태로의 제작이 가능하여 세밀한 촉각 정보를 궤환시켜 줄 수 있는 새로운 촉각궤환장치 기구(mechanism)를 제안하였다. 이 기구는 전자석의 흡인력을 이용한 것이라 손가락 피부에 부착할 만큼 충분히 소형, 경량이며, 연속적인 선형 제어가 용이하고, 단위 크기당 비교적 큰 힘을 낼 수 있으며, 사용시 부작용이 없는 특징을 갖고 있다. 최적설계에 있어서는 최적설계의 목적함수를 촉각인식기구의 고찰을 통해 구하였고, 외형의 기하하적 관계와 인가 전류에 의해 발생되어진 온도조건을 구속조건으로 삼아 가장 큰 촉각력을 발생시킬 수 있도록 최적 설계를 수행하였다. 촉각력 계산은 가정자로법과 FEM(finite element method)을 통해 구하였다. 최적화 결과는 이전 제작 샘플보다 24%의 향상이 있음을 알 수 있었다. In a teleoperation robot system, it is important for an operator to feel as if he really were in a distant place. To realize this objective, the various information from remote site must be presented to the operator. Even though tactile information is very important to execute the task efficiently, it is not yet sufficiently provided for the operator. In this paper, it is proposed the new mechanism which provides the more dexterous tactile information to an operator. This device utilizes the electromagnetic force, so that it is small and light enough to attach on the fingerpad. It can also be ontrolled continuously. The magnetic circuit is derived by the probable flux paths method, to solve the force at any given dimension. The object of optimization is to maximize the tactile force which human can perceive under same conditions. the objective function is formulated as maximizing indentation displacement on the fingerpad in consideration of the mechanism of human tactile perception. The optimization formulation is subjected to geometric constraint and the rising temperature constraint in the coil. As a result of optimization, 24 % increases in the tactile force are accomplished, compared with the initial design.

馬山灣에서의 燐과 窒素의 動態에 관하여

안태석,안태영,권오섭,조기성 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

1978년 부터 1982년 까지 마산만 4개 정점에서 인산에 인과 질소 상태에 대한 조사 결과, 인산태 인과 질소 상태에 대한 조사 결과, 인산태 인은 각 정점별 평균치가 0.86∼2.70 μg at - P/ℓ이였고, Ammonia + amino 산태 질소는 1.21 ∼4.36 μg at - L/ℓ이였고, 질산태 질소는 0.94 ∼ μg at - N/ℓ 로 비교적 높은 값을 보였다. 마산시에 인접한 정점 1, 2에서는 질소의 농도가 점차로 증가하고 있는 경향을 보였다. 또한 질소와 인의 비율은 정점 1과 2에서 40을 넘는 값을 보여주어, 질소의 유입이 큰 것을 뜻하므로 이에대한 방지대책이 시급히 요구된다. The concentrations of phosphate-P, ammonia + amino acid-N, nitrite-N, and nitrate-N were measured at 4 sites in the polluted Masan Bay from 1978 to 1982. The mean concentration of phosphate-P, ammonia + amino acid-N, nitrine-N and nitrate-N in each site varied 0.68-1.90 ug at P/1, 1.21-4.36 ug at-N/1, 0.36-3.23 ug at-N/1 and 2.17-29.45 ug at-N/1, respectively. At site 1 and 2, nearyby Masan City, the concentration of nitrogen was gradually increasing according to the increase of the population of the city and the N/P ratios at site 1 and 2 reached more than 40.

漢江上流 人工湖에서의 微生物 分布와 活性度에 관하여

안태석,이동훈,김범철,조규송 江原大學校 附設 環境硏究所 1989 環境硏究 Vol.6 No.-

The values of total hacterial numbers, heterotrophic bacterial numbers, the proportions of specific enzyme releasing bacteria and microbial activities were measured in the five artificial reservoirs in North Han river. The values of total bacterial number ranged from 0.62x10E5cells/ml, which is the common values in mesotrophic lakes. With the effect of civil construction at the upper region of the North Han river, the proportions of alpha-glucosidase-releasing bacteria were higher in Lake Paro and Lake Chuncheon. Beta-glucosidase proportions were high in Lake Paldang and showed high correlation with the concentration of chlorophyll a. The alkaline phophlatase proportion were higher in Lake Paro, Lake Chuncheon and Lake Paldang. The turnover rate of acetate showed no relationship with any other trophic state index intems, such as chlorophyll concentration, phosphate concentration and primary productivity. But the phosphatase activity showed high correlation coefficience, and it should be estimated as a trophic state index in these artificial reservoirs.

캡슐 발효유에 의한 변비 개선

안태석 江原大學校 附設 環境硏究所 1999 環境硏究 Vol.16 No.-

유산균 발효유 중 캡슐 제제를 사용한 경우 변비 증세가 개선되는가를 확인하였다. 변비의 자각증세가 있는 20명에게 하루 2회씩 캡슐 제제가 포함된 유산균 발효유를 14일간 복용하게 하고, 복용 중단 후 14일간 배변 상태 등을 설문하였다. 그 결과 음용기간 중 변비가 상당히 개선된 경우는 13명(65%), 조금 개선된 경우는 5명(25%)로 총18명(90%)가 변비가 조금 이상 개선되었다. 또 음용 중단 이후를 관찰한 결과, 상당히 개선된 경우가 16명 (80%)이며, 조금 개선된 경우는 3명(15%)로 총 19명(95%)이 개선되었다. 또 변의 상태가 음용 전후와 비교하여 부드러워진 경우는 15명(75%)으로 나타났다. 음용 후 복부의 긴장감과 불쾌감은 초기에는 많이 나타났으나, 음용 후반기에 들어서면서 대부분의 실험자가 없다고 대답하였다. 이 조사에서 캡슐제제의 유산균 발효유는 변비 개선에 상당한 효과가 있으며, 음용 중단 후에도 2주간지속되는 것으로 나타났다. Evaluation of capsulated yogurt for bowels was carried out. To the twenty volunteers who has selfconsciousness of constipation, the two bottles of capsulated yogurt were applied daily for two weeks. By the results, totally 90% of volunteers replied that their constipation was loosing during the application and 95% after the 2 weeks of stopping the application.

소양호에서 세균군집 크기에 미치는 폴랑크톤의 영향

안태석,정미정,이은주,조규송 江原大學校 附設 環境硏究所 1996 環境硏究 Vol.13 No.-

To scrutinize the biological effects of plankton communities to bacterial community, the total bacterial numbers, chlorophyll a concentration and phytoplankton, zooplankton communities were analyzed in Lake Soyang from May 13, to July 14, 1994. At 0m depth, total bacterial numbers positively coi-related with chlorophyll o concentration. On the other hand, at 2m depth, the two parameters had negative correlation. At the middle of the June, whe dominants of phytoplankton, zooplankton were changing, the bacterial numbers dropped down. These results suggest that as the Anabaena becoming dominant species, the some zooplankton species changed to bacterivore. The bacterial community was mainly effected by phytoplankton at 0m, and by zooplankton at 2m depth.

TiO₂ 졸-겔 코팅 막에 의한 Humic Acid의 광분해 : 화학적 산화법에 의한 부식산의 분해처리 기술에 관한 연구(Ⅱ);Characterization of Humic Acid in the Chemical Oxidation Treatment (Ⅱ)

석상일,안복엽,서태수,이동석 江原大學校 附設 環境硏究所 2000 環境硏究 Vol.17 No.-

The degradation of humic acid using TiO₂ coatings studied. TiO₂ coatings were prepared by dip-coating method. Sol solutions for coating were prepared by mixing the gel, which can be produced by the reaction of TiOCl₂ and NH₁OH solution, and hydrogen peroxide solution, and hydrolysis of titanium tetraisopropoxide (TTIP). It was shown from SRD that coatings from sol aged at 100℃ for 18h with titanium peroxo solution were crystallized to anatase in the range of temperatures of 25℃ to 500℃. In contrast, those coated from TTIP were crystallized to anatase at temperature above 400℃. So the sols originated from TiCl₄ can be applied for not only on the heat-resistance substrates but on the plastic substrates. Thickness and the quality of the films were dependent on the withdrawing speed, the concentration of sol, and the number of coating. The films showed various interference colors depending on the thickness of them. In the case that the films coated 2 times at withdrawing speed of 2.5cm per minute by 0.2M sol, the films had a transparent light blue color with thickness of around 50nm. It was known from the result of photo-degradation by TiO₂ coatings using humic acid that the removal efficiency of COD_(cr) was over 85% after illumination of UV/H₂O₂ for 40min, and that of UV/VIS absorbable materials was over 95%. TiO₂코팅매체를 이용한 humid acid의 광분해 특성을 조사하였다. TiO₂코팅은 TiOCl₂ 수용액을 암모니아수로 침전시킨 겔을 과산화수소로 용해한 용액 혹은 졸이나 titanium tetraisopropoxide (TTIP)의 가수분해로부터 제조한 졸을 이용하여 dip-coating법으로 제조하였다. Titanium peroxo 용액을 열처리하여 제조한 졸을 이용한 코팅층은 X-선 회절 분석으로부터 25℃~500℃ 온도 범위에서 모두 anatase형 결정구조를 가지고 있었다. 반면에 TTIP의 가수분해로 생성된 졸로부터 만든 코팅막은 400℃ 이상에서 anatase의 결정형이 나타났다. 이로부터 titanium peroxo 용액을 열처리하여 제조한 졸은 내열성 및 비내열성 기판에도 결정성 TiO₂ 코팅층을 만들 수 있는 장점이 있다. 코팅막의 두께 및 균일성은 인출속도, 코팅졸의 농도 및 코팅 횟수에 영향을 받았으며, 코팅막의 두께에 따라 다양한 간접색상을 나타냈다. 0.2M 졸을 이용하여 인출속도 2.2cm/min로 2회 코팅했을 경우, 약 50nm 두께의 투명하면서도 균일한 흐린 남색을 띠는 TiO₂코팅막을 얻을 수 있었다. 이상의 방법으로 직경 0.3cm의 유리구슬에 TiO₂ 코팅막을 제조한 후 580cm³의 반응조를 사용하여 UV/H₂O₂ 공정으로 humic acid를 40분 동안 광반응시킨 결과, 초기 시료의 COD_(cr)(40ppm)을 약 85% 이상, 흡광물질을 약 95% 이상 제거하였다.