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Assay of Trace Gold Ion in a Skin Cell Using a Stripping Voltammetry
( Suw Young Ly ),( Jin Hui Lee ),( Jae Hun Yi ) 한국유화학회 2011 한국응용과학기술학회지 Vol.28 No.1
Threelectrodes systems were used in stripping voltammetry (SW) and cyclic voltammetry (CV) instead of the expensive platinum and Ag/AgCl reference electrodes. Moreover, the electrolyte solution was used with deep seawater, which can reduce water pollution, is more eco-friendly, and has a lower cost. The analytical optimum parameters measured via CV and SW and with working ranges were obtained from 10 to 80 ug/L using fluorine immobilized on a graphite pencil electrode (FE). Under the optimum conditions, the analytical detection limit of 6.30 ug/LAu was obtained. The results of the study can be applied to diagnostic assay for natural minerals and human finger tissue.
Young Ly, Suw,Keun Kim, Jong Wiley Subscription Services, Inc., A Wiley Company 2009 Journal of biochemical and molecular toxicology Vol.23 No.4
<P>The electrical circuit of an infrared photodiode electrode (IPE) was used in the simultaneous assay of copper and cadmium ions. The electrode's cyclic voltammetry (CV), chronoamperometry and square-wave (SW) stripping voltammetric optimum conditions were examined. Results for 0–160 mg L<SUP>−1</SUP> and 50–400 μg L<SUP>−1</SUP> SW Cu(II) Cd(II), the relative standard deviation of 0.158 Cu(II), 0.077 Cd(II) (n = 15) using 20.0 mg L<SUP>−1</SUP> have been obtained at optimum conditions. The low detection limit (S/N) was attained to be at 14.71 μg L<SUP>−1</SUP>(2.31 × 10<SUP>−7</SUP> mol L<SUP>−1</SUP>) Cu(II) and 18.42 μg L<SUP>−1</SUP>(1.63 × 10<SUP>−7</SUP> mol L<SUP>−1</SUP>) Cd(II). The handmade electrode was implanted deep in the muscle of live fish and interfaced with an electrochemical workstation. Real-time analytical application was performed on the online assay of living tissue as the specimen was moving. The methods are deemed useful in interfaced assay for physiological control, nanodiode fabrication, and in the production of laboratory on a biochip. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:256–262, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20287</P>
Radioactive uranium measurement in vivo using a handheld interfaced analyzer.
Ly, Suw Young,Lee, Jin-Hui,Jung, Dong Ho Pergamon 2010 Environmental toxicology and chemistry Vol.29 No.5
<P>A trace uranium (U) detection method was developed with a handheld voltammetric analyzer that was the size of a mobile phone, with working sensors made of simple graphite pencil electrode (PE). The optimum stripping voltammetric conditions were sought, and the following results were obtained: 0.0 to 0.08 ng/L working ranges and a statistically relative standard deviation of 1.78% (RSD; n=15) at a 10.0 microg/L U spike. The experiment accumulation time used was only 150 s. Under this condition, the diagnostic detection limit approached 0.007 ng/L. The method was applied to soil of a natural rock in a radioactive mineralogy site. Earthworms that resided at this site were assayed. The method was found to be applicable in biological diagnosis or in real-time in vivo survey.</P>
Administering Pesticide Assays in In Vivo-Implanted Biosensors
Ly, Suw Young,Jung, Young Sam,Lee, Chang Hyun,Won Lee, Bang CSIRO Publishing 2008 Australian journal of chemistry Vol.61 No.10
<P> An analytical pesticide assay of O-ethyl-O-4-(nitrophenyl)phenyl phosphonothioate (EPN) was carried out using the following: a carbon nanotube paste electrode, a mercury-immobilized carbon nanotube paste electrode, a glassy carbon electrode, a metal-gold electrode, and a DNA-immobilized carbon nanotube paste electrode (DPE), which is two-fold more sensitive than other sensors. The DPE was optimized using cyclic and square wave stripping voltammetry. Linear working ranges approached 5-55 mg L-1 EPN and the nano-range of 10-210 ng L-1 in a 0.1 mol L-1 NH4H2PO4 electrolyte solution, with a speedy analytical time of 30-s stripping. The detection limit was 2.57 ng L-1 (7.94 × 10-12 mol L-1), and the precision was 0.102% relative standard deviation (n = 15) at the 10.0 mg L-1 EPN spike. This indicates that the method is more sensitive than common voltammetric methods. This method was applied to fruit samples using patch- and needle-type electrodes, specifically on the skin tissues of an orange and an apple. Moreover, the implanted electrode was interfaced with a fish brain cell at the electrochemical workstation. Results showed that the aforementioned method can be used to conduct a pesticide assay in neuro-treated and non-treated cell systems. </P>
Antibiotics Assay of Doxycycline in Food System using Stripping Voltammetry
Suw Young Ly,Chang Hyun Lee 한국유화학회 2016 한국응용과학기술학회지 Vol.33 No.4
A voltammetric analysis of doxycycline was developed using DNA immobilized onto a carbon nanotube paste electrode (PE). An anodic peak current was indicated at 0.2 V (versus Ag/AgCl) in a 0.1M NH4H2PO4 electrolyte solution. The linear working range of the cyclic and square wave stripping voltammetry was obtained to 1-27 ngL-1 with an accumulation time of 800s. Final analytical parameters were optimized to be as follows: amplitude, 0.35 V; frequency, 500 Hz; and pH, 5.43. Here detection limit was found to be 0.45 ngL-1, this result can be applied in foods systems and in the biological diagnostics
Analysis of Lead Ions in a Waste Solution Using Infrared Photo-Diode Electrode
Ly, Suw-Young,Lee, Hyun-Kuy,Kwak, Kyu-Ju,Ko, Jun-Seok,Lee, Jeong-Jae,Cho, Jin-Hee,Kim, Ki-Hong,Kim, Min-Seok,Lee, So-Jung Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol.25 No.4
To detect lead ions using electrochemical voltammetric analysis, Infrared Photo-Diode Electrode(IPDE) was applied via cyclic and square wave stripping voltammetry. Lead ions were deposited at 0.5 V(versus Ag/AgCl) accumulation potential. Instrumental measurements systems were made based on a simple and compact detection system. The stripping voltammetric and cyclic voltammetric optimal parameters were searched. The results yielded a cyclic range of $40{\sim}240mgl^{-1}$ Pb(II) and a square wave stripping working range of $0.5{\sim}5.00mgl^{-1}$ Pb(II). The relative standard deviation at 2 and 4 $mgl^{-1}$ Pb(II) was 0.04% and 0.02%(n=15), respectively, using the stripping voltammetric conditions. The detection limit was found to be 0.05 $mgl^{-1}$ with a 40 sec preconcentration time. Analytical interference ions were also evaluated. The proposed method was applied to determine lead ions in various samples.
Voltammetric Assay of Mercury Ion in Fish Kidneys
Ly, Suw-Young Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.
Voltammetric analysis of mercury ions was developed using paste electrodes (PEs) with DNA and carbon nanotube mixed electrodes. The optimized analytical results of the cyclic voltammetry (CV) of the $1{\sim}14ng\;L^{-1}Hg(II)$ concentration and the square wave (SW) stripping voltammetry of the $1{\sim}12ng\;L^{-1}Hg(II)$ working range within an accumulation time of 400 seconds were obtained in 0.1 M $NH_4H_2PO_4$ electrolyte solutions of pH 4.0. For the relative standard deviations of the $1ng\;L^{-1}Hg(II)$, which were observed at 0.078% (n = 15) at the optimum conditions, the low detection limit (S/N) was pegged at $0.2ng\;L^{-1}(7.37{\times}10^{-13}M)$ for Hg(II). The results can be applied to assays in biological fish kidneys and wastewater samples.
Simultaneous Diagnostic Assay of Catechol and Caffeine Using an in vivo Implanted Neuro Sensor
Ly, Suw-Young,Lee, Chang-Hyun,Jung, Young-Sam,Kwon, O-Min,Lee, Ji-Eun,Baek, Seung-Min,Kwak, Kyu-Ju Korean Chemical Society 2008 Bulletin of the Korean Chemical Society Vol.29 No.9
Catechol and caffeine were simultaneously analyzed with a bismuth-immobilized carbon nanotube paste electrode (BPE) using square wave (SW) stripping voltammetry. Optimum analytical conditions were determined. Simultaneous working ranges of 100-1,500 $mgL^{-1}$ for caffeine and 5-75 $mgL^{-1}$ for catechol were obtained. In the separated cell systems, a working range of 0.1-2.1 $mgL^{-1}$ catechol with a correlation coefficient of 0.9935, and a working range of 10-210 $mgL^{-1}$ caffeine with a correlation coefficient of 0.9921 were obtained. A detection limit (S/N) of 0.15 $mgL^{-1}$ (7.7 ${\times}$ $10^{-7}$ M) and a detection limit of 0.02 $mgL^{-1}$ (1.82 ${\times}$ $10^{-7}$ M), respectively, manifested for catechol and caffeine. It was found that three macro-type electrode systems could be implanted in fish and rat neuro cells. For both ions, the ion currents were observed. The physiological impulse conditions and the neuronal thinking current were also obtained.