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DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins
Woo, Je Wook,Kim, Jungeun,Kwon, Soon Il,Corvalá,n, Claudia,Cho, Seung Woo,Kim, Hyeran,Kim, Sang-Gyu,Kim, Sang-Tae,Choe, Sunghwa,Kim, Jin-Soo Nature Publishing Group, a division of Macmillan P 2015 Nature biotechnology Vol.33 No.11
Editing plant genomes without introducing foreign DNA into cells may alleviate regulatory concerns related to genetically modified plants. We transfected preassembled complexes of purified Cas9 protein and guide RNA into plant protoplasts of Arabidopsis thaliana, tobacco, lettuce and rice and achieved targeted mutagenesis in regenerated plants at frequencies of up to 46%. The targeted sites contained germline-transmissible small insertions or deletions that are indistinguishable from naturally occurring genetic variation.
Son, Jeong-Yu,Kim, Sunghwa,Jeon, Woo Hyung,Lee, Phil Ho American Chemical Society 2015 ORGANIC LETTERS Vol.17 No.10
<P>A synthetic method of a wide range of cinnolin-3(2<I>H</I>)-one derivatives is developed from the reaction of symmetrical as well as unsymmetrical azobenzenes with diazotized Meldrum’s acid <I>via</I> Rh-catalyzed C–H alkylation followed by cyclization.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2015/orlef7.2015.17.issue-10/acs.orglett.5b01052/production/images/medium/ol-2015-01052h_0012.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol5b01052'>ACS Electronic Supporting Info</A></P>