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Choi, Dukhyun,Choi, Min-Yeol,Choi, Won Mook,Shin, Hyeon-Jin,Park, Hyun-Kyu,Seo, Ju-Seok,Park, Jongbong,Yoon, Seon-Mi,Chae, Seung Jin,Lee, Young Hee,Kim, Sang-Woo,Choi, Jae-Young,Lee, Sang Yoon,Kim, Jo WILEY-VCH Verlag 2010 ADVANCED MATERIALS Vol.22 No.19
<B>Graphic Abstract</B> <P>The cover shows an image of fully rollable transparent nanogenerators synthesized using chemical vapor deposition grown large-scale graphene sheets as transparent electrodes and piezoelectric ZnO nanorod arrays. Sang-Woo Kim, Jae-Young Choi, and co-workers report on p. 2187 the electrical and structural stability of the nanogenerators, with excellent charge scavenging performance under external mechanical loads such as bending and rolling. This study shows that graphene-based nanogenerators are very promising for self-powered rollable transparent device applications. <img src='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content.gif' alt='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content'> </P>
Sang-yun Yang,Yonggun Park,Hyunwoo Chung,Hyunbin Kim,Se-yeong Park,In-gyu Choi,Ohkyung Kwon,Kyu-chae Cho,Hwanmyeong Yeo 한국목재공학회 2017 목재공학 Vol.45 No.4
Research on the rapid and accurate prediction of physical properties of wood using near-infrared (NIR) spectroscopy has attracted recent attention. In this study, partial least squares analysis was performed between NIR spectra and air-dried specific gravity of five domestic conifer species including larch (Larix kaempferi), Korean pine (Pinus koraiensis), red pine (Pinus densiflora), cedar (Cryptomeria japonica), and cypress (Chamaecyparis obtusa). Fifty different lumbers per species were purchased from the five National Forestry Cooperative Federations of Korea. The air-dried specific gravity of 100 knot- and defect-free specimens of each species was determined by NIR spectroscopy in the range of 680-2500 nm. Spectral data preprocessing including standard normal variate, detrend and forward first derivative (gap size = 8, smoothing = 8) were applied to all the NIR spectra of the specimens. Partial least squares analysis including cross-validation (five groups) was performed with the air-dried specific gravity and NIR spectra. When the performance of the regression model was expressed as R<sup>2</sup> (coefficient of determination) and root mean square error of calibration (RMSEC), R<sup>2</sup> and RMSEC were 0.63 and 0.027 for larch, 0.68 and 0.033 for Korean pine, 0.62 and 0.033 for red pine, 0.76 and 0.022 for cedar, and 0.79 and 0.027 for cypress, respectively. For the calibration model, which contained all species in this study, the R<sup>2</sup> was 0.75 and the RMSEC was 0.37.
( Chae June Lim ),( Chung-hwan Jun ),( Sung-bum Cho ),( Sung-kyu Choi ),( Ji-yun Hong ) 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1
Aims: Pulmonary thromboembolism may be the first manifestation of an occult malignancy and pulmonary infarction can occur when peripheral branches of pulmonary artery are blocked by thrombosis. We have experienced a rare case of hepatocellular carcinoma that first appeared as pulmonary embolism and infarction. Results: A 74-year-old man was admitted for cough, small amount of hemoptysis and sudden onset dyspnea. He had history of gout and was a chronic alcohol drinker. On admission, his vital signs were 37.3 ℃-140/90 mmHg-108 beats/min, 22 breaths/min and the oxygen saturation 91% while he was breathing in room air. Laboratory data showed CBC 12,700 /㎣-15.0 g/㎗-247,000/㎣, CRP of 7.21 ㎎/㎗, normal serum bilirubin, INR, troponin and D-dimer level. Chest CT angiography was performed and found thrombosis in left main, lobar and segmental pulmonary arteries and wedge-shaped consolidations of the lobes responsible for these arteries. Thus, pulmonary embolism and associated pulmonary infarction were diagnosed. And abdominal CT dynamic study revealed 9.9x5.2㎝ sized low attenuated mass in S7 of the liver with invasion of right portal vein, hepatic vein and IVC. Echocardiography showed no intracardiac thrombus without sign of right ventiricular dysfunction. So the final diagnosis was hepatocellular carcinoma with angioinvasion, thromboembolism and subsequent pulmonary infarction. Anticoagulation therapy with low-molecular weight heparin was administered, and transarterial chemoembolization was performed as a fundamental treatment. Until now, there has been no sign of worsening of disease and the patient showed improvement in symptoms. Conclusions: We report a rare case of hepatocellular carcinoma that presented as pulmonary thromboembolism and subsequent pulmonary infarction as the first manifestation.
Chae, Tong Un,Ko, Yoo-Sung,Hwang, Kyu-Sang,Lee, Sang Yup Elsevier 2017 Metabolic engineering Vol.41 No.-
<P><B>Abstract</B></P> <P>Microbial production of chemicals and materials from renewable sources is becoming increasingly important for sustainable chemical industry. Here, we report construction of a new and efficient platform metabolic pathway for the production of four-carbon (butyrolactam), five-carbon (valerolactam) and six-carbon (caprolactam) lactams. This pathway uses ω-amino acids as precursors and comprises two steps. Activation of ω-amino acids catalyzed by the <I>Clostridium propionicum</I> β-alanine CoA transferase (Act) followed by spontaneous cyclization. The pathway operation was validated both <I>in vitro</I> and <I>in vivo</I>. Three metabolically engineered <I>Escherichia coli</I> strains were developed by introducing the newly constructed metabolic pathway followed by systems-level optimization, which resulted in the production of butyrolactam, valerolactam and caprolactam from renewable carbon source. In particular, fed-batch fermentation of the final engineered <I>E. coli</I> strain produced 54.14g/L of butyrolactam in a glucose minimal medium. These results demonstrate the high efficiency of the novel lactam pathway developed in this study.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lactams are important chemicals having a wide range of industrial applications. </LI> <LI> Novel metabolic pathways for four-, five- and six-carbon lactams are constructed. </LI> <LI> β-Alanine CoA transferase is a key enzyme for the novel pathway. </LI> <LI> Production of C4, C5 and C6 lactams from renewable carbon sources are demonstrated. </LI> <LI> Fed-batch fermentation allowed production of 54g/L butyrolactam. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Sung-Kyu Chae,Hyo-Jin Park,Jin-Woo Kim,Jae-Hyun Ahn,Soo-Yong Park,Jae-Young Park,Seul-Gi Yang,Deog-Bon Koo 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.4
Gangliosides exist in glycosphingolipid-enriched domains on the cell membrane and regulate various functions such as adhesion, differentiation, and receptor signaling. Ganglioside GM3 by ST3GAL5 enzyme provides an essential function in the biosynthesis of more complex ganglio-series gangliosides. However, the role of gangliosides GM3 in porcine oocytes during in vitro maturation and early embryo development stage has not yet understood clear. Therefore, we examined ganglioside GM3 expression patterns under apoptosis stress during maturation and preimplantation development of porcine oocytes and embryos. First, porcine oocytes cultured in the NCSU-23 medium for 44 h after H2O2 treated groups (0.01, 0.1, 1 mM). After completion of meiotic maturation, the proportion MII (44 h) was significantly different among control and the H2O2 treated groups (76.8±0.3 vs 69.1±0.4; 0.01 mM, 55.7±1.0; 0.1 mM, 38.2±1.6%; 1 mM, P<0.05). The expressions of ST3GAL5 in H2O2 treated groups were gradually decreased compared with control group. Next, changes of ST3GAL5 expression patterns were detected by using immunofluorescene (IF) staining during preimplantation development until blastocyst. As a result, we confirmed that the expressions of ST3GAL5 in cleaving embryos were gradually decreased (P<0.05) according to the early embryo development progress. Based on these results, we suggest that the ganglioside GM3 was used to the marker as pro-apoptotic factor in porcine oocyte of maturation and early embryo production in vitro, respectively. Furthermore, our findings will be helpful for better understanding the basic mechanism of gangliosides GM3 regulating in oocyte maturation and early embryonic development of porcine in vitro.
Sung-Kyu Chae,박효진,Jin-Woo Kim,Jae-Hyun Ahn,Soo-Yong Park,Jae-Young Park,Seul-Gi Yang,구덕본 사단법인 한국동물생명공학회 2015 한국동물생명공학회지 Vol.30 No.4
Gangliosides exist in glycosphingolipid-enriched domains on the cell membrane and regulate various functions such as adhesion, differentiation, and receptor signaling. Ganglioside GM3 by ST3GAL5 enzyme provides an essential function in the biosynthesis of more complex ganglio-series gangliosides. However, the role of gangliosides GM3 in porcine oocytes during in vitro maturation and early embryo development stage has not yet understood clear. Therefore, we examined ganglioside GM3 expression patterns under apoptosis stress during maturation and preimplantation development of porcine oocytes and embryos. First, porcine oocytes cultured in the NCSU-23 medium for 44 h after H2O2 treated groups (0.01, 0.1, 1 mM). After completion of meiotic maturation, the proportion MII (44 h) was significantly different among control and the H2O2 treated groups (76.8±0.3 vs 69.1±0.4; 0.01 mM, 55.7±1.0; 0.1 mM, 38.2±1.6%; 1 mM, P<0.05). The expressions of ST3GAL5 in H2O2 treated groups were gradually decreased compared with control group. Next, changes of ST3GAL5 expression patterns were detected by using immunofluorescene (IF) staining during preimplantation development until blastocyst. As a result, we confirmed that the expressions of ST3GAL5 in cleaving embryos were gradually decreased (P<0.05) according to the early embryo development progress. Based on these results, we suggest that the ganglioside GM3 was used to the marker as pro-apoptotic factor in porcine oocyte of maturation and early embryo production in vitro, respectively. Furthermore, our findings will be helpful for better understanding the basic mechanism of gangliosides GM3 regulating in oocyte maturation and early embryonic development of porcine in vitro.
Practical Application of Sn-3.0Ag-0.5Cu Lead Free Solder in Electronic Production
Chae Kyu-Sang,Min Jae-Sang,Kim Ik-Joo,Cho Il-Je The Korean Microelectronics and Packaging Society 2005 마이크로전자 및 패키징학회지 Vol.12 No.1
At present, Electronic industries push ahead to eliminate the Pb(Lead) -a hazardous material-from all products. Especially, we have performed to select the optimum standard composition of lead free alloy for the application to products for about 3 years from 2000. These days, we have the chance for applying to the mass-production. This project constructed the system for applying the lead free solders on consumer electronic products, which is one of the major products of the LG Electronics. To select the lead free solders with corresponding to the product features, we have passed through the test and applied with Sn-3.0Ag-0.5Cu alloy system to our products, and for the application to the high melting temperature composition, we secured the thermal resistance of the many parts and substrate and optimized the processing conditions. We have operated the temperature cycling test and the high temperature storage test under the standards to confirm the reliability of the products. On these samples, we considered the consequence of our decision by the operating test. For the long life time of the product, we have operated the temperature cycling test at $-45^{\circ}C\;-\;+125^{\circ}C$, 1 cycle/hour, 1000 cycles. Also we have tested the tin whisker growth about lead free plating on lead finish. We have analyzed with the SEM, EDS and any other equipment for confirming the failure mode at the joint and the tin whisker growth on lead free finish.