VP-16으로 성공적으로 치료된 위 Kaposi 육종 1예

양웅석,송근암,조몽,이재승,송철수,김형욱,김영민,이수봉,김태오,이성훈 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

The skin is an uncommon site of distant metastasis from any internal malignancy, and the incidence of metastatic skin lesions as the first symptom of disease is only 0.8% in patients with all systemic malignancies. Furthermore, cutaneous metastasis from adenocarcinoma of the stomach has been found to be extremely rare. A 35-year-old female patient was admitted due to multiple cutaneous nodules in her chest, abdomen, and back. A gastroendoscopic examination and biopsy was made according to the results of skin pathologic findings. Stomach and skin biopsy results revealed a signet ring cell type of adenocarcinoma. A case of gastric adenocarcinoma in which metastatic skin nodules appeared as the first sign of disease, is here in reported with a review of related literature.

Anthraquinone계 화합물의 변이원성 및 유전독성 발현에 관한 연구(I) : Mutagenicity of emodin activated with cytochrome P-450 Cytochrome P-450에 의해 활성화된 emodin의 변이원성

李雄洙 忠州大學校 1998 한국교통대학교 논문집 Vol.33 No.2

It has been reported that several anthraquinone compounds possess mutagenicity and carcinogenicity. Emodin is one of the anthraquinone mycotoxins, and a constituent of several herb medicine used as laxative such as Rhei Rhizoma(rhubarb), Cassiae Semen(cassia seed) and Aloe. Emodin was selected as a model compound to solve a carcinogenic mechanism of the anthraquinone mycotoxins and to evaluate the safety of herb medicine. In this research, the mutagenicity and metabolic reaction of emodin were investigated with the purified cytochrome P-450s. In Ames test with the reconstituted cytochrome P-450 system derived from the hepatic microsomes of PB-induced rats, the mutagenicity of emodin activated with the fractionⅡ(P-448 type) was significantly higher than with the fraction Ⅰ(P-450 type). TLC analysis of the metabolites revealed that among 8 isozymes of P-450 type(Ⅰ-a, b, c, d) and P-448 type(Ⅱ-a, b, c, d), only ω-OH-emodin was detected on the TLC plate when emodin was incubated with 4 isozymes of P-450 type(Ⅰ-a, b, c, d). Whereas, 2-, 4-, 7-, ω-OH-emodin were detected in the metabolites produced by P-448 type(Ⅱ-a, b, c, d). Of 8 isozymes, Ⅱ-a possessed the highest activity in the production of 2-OH-emodin, a direct-acting mutagen. This Ⅱ-a was also represented to catalyze the hydroxylation of aflatoxin B1 and ochratoxin A into aflatoxin M1 and 4(R)-4-hydroxy-ochratoxin A, respectively. It was suggested that P-448 types play an important role in the metabolic activation of emodin.

장내세균에 의한 Trichothecene Mycotoxins의 대사 : (I) T-2 toxin의 대사

이웅수 忠州大學校 2008 한국교통대학교 논문집 Vol.43 No.-

Trichothecene mycotoxins are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. Especially, T-2 toxin is a secondary metabolite produced by Fusarium spp. such as F. tricinctum, F. poae and F. sporotrichioides etc., and is often found in agricultural products and feeds including cereals, and is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA), and induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to elucidate the possible metabolism of T-2 toxin by rat and human intestinal bacteria, this research was carried out. T-2 toxin transformed into HT-2 toxin and unknown metabolite by cultured intestinal bacteria in the anaerobic condition. The amount of HT-2 toxin was decreased, but unknown metabolite was increased by subsequent incubation. This metabolite was elucidated to deepoxy HT-2 toxin by NMR and mass spectra data. It was suggested that T-2 toxin was deepoxidized after deacetylation to HT-2 toxin by the intestinal bacteria. This deepoxy HT-2 toxin was transformed into HT-2 toxin and 15-deacetyl-deepoxy HT-2 toxin by liver microsome of PCB-treated rat in the presence of NADPH and oxygen. These results suggest that the epoxide group of trichothecene mycotoxins is reduced by intestinal bacteria and re-oxidized by hepatic microsomal enzyme of rat.

중ㆍ장거리 선수의 최대산소섭취량, 무산소성 역치 및 혈액성분에 관한 연구

이영웅,박수연,박철빈 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The purpose of this paper was evaluate the specificity of maximal oxygen uptake and anaerobic threshold which is indicator of cardio- respiratory function during incremental load exercise using treadmill, also. Twenty of elite middle and long distance runners participated as subjects of this research. By measurement the specificity of cardio-fulmonary function and blood component in middle and long distance runners, the conclusion and results which is obtained is as follows: 1.Maximal oxygen uptake of absolute and relative value during maximal exercise load in middle and long distance runners was display 4.23±0.421/min & 71.2/㎖/kg/min, respectively, also, maximal ventilation and maximal heart rate was expressed 146± 18.861/min & 192.25 ± 7.61beats/min, respectively. 2.Oxygen uptake and relative percentage of maximal oxygen uptake in the anaerobic threshold levels of middle and long distance runners was shown 3.55± 0.491/min(46.54㎖/kg/min) 72.81±8.86%, respectively and ventilation, heart rate and relative percentage of maximal heart rate was expressed 95.09 ± 16.951/min, 176.54 ± 9.79beats/min and 91.62 ±3.90%, respectively. 3.Concentration of plasma lactate(t-value:-3.65, p<0.003), glucose (t-value:-4.52, p<0.02) In blood component of middle and long distance runners was display the significantly difference between pre and post value after maximal incremental exercise load.

人體重心과 體格 및 體力에 關한 硏究(Ⅰ) : 國民學生 5 ·6學年을 中心으로

李永雄,朴秀一 慶尙大學校 1980 論文集 Vol.19 No.1

1. The purpose of this study is to investigate the height of the center of gravity of a body according to physique, and is also to investigate the difference of physical fitness by the physique according to the height of the center of gravity of a body. 2. Physique was measured by the height and weight of the subjects and classified into five ranks of physical progressions, and then the subjects were tested in five items; 100m sprint, standing broad jump, flexed arm-hamging(female) and pull-ups(male), ball throwing and sit-up. Next, the position of the center of gravity of a body was measured by "the method fo Reynolds-Lovett". 3. In the height of the center of gravity of a body, the ranks of the height were as follows; A class was the lst, B class was the 2nd, C class was the 3rd, D class was the 4th, and E class was the 5th. 4. The average height of the center of gravity of a body by age and sex might be summarized as follows. The center of 10 year-old girls was located in 56.21% point of the standing height. That of 11 year-old girls was located in 57.51/%point. That of 10 year-old boys was located in 57.24% point. That of 11 year-old boys was located in 57.93% point. Therefore, the center of gravity of a body in case of girls was situated in almost the same height as in case of boys and was higher than in case of grown-up women. 5. In physical fitness, E class was a little inferior to the other classes, but any difference among the other classes was not found out.

수입 중국산 개나리 열매(연교)의 소염효과에 관한 연구

이응식,조재민,민윤식 충주대 산업과학기술연구소 1996 産業科學論文集 Vol.4 No.-

In order to determine whether methanol extract of Chinese Forsythiae fructus inhibite acute and chronic inflammatory responses induced with histamine and carrageenin in rat, this research was carried out. Inhibitory effect on the pigment leakage induced with histamine 50㎍ by methanol extract 0.15g per 150g body weight was shown positive result significantly. In the experiment by carrageenin paw edema method, maximum inhibitory effects were shown 1hr and 30min after orally administrated with 0.03g and 0.3g methanol extract per 150g body weight, respectively. In the inhibitory effect by methanol extract on the acute inflammation induced by granuloma pouch method, it was shown that inflammatory exudate of granuloma pouch and wet weight of granulation tissue reduced. In the chronic inflammation by granuloma pouch method, inhibitory effect was observed but significant inhibition was not observed statistically. The results of this research were shown that methanol extract of Chinese Forsythiae fructus has inhibitory effects on the acute and chronic inflammation, and were similar to those reported on Korean Forsythiae fructus.

Anthraquinone계 화합물의 변이원성 및 유전독성 발현에 관한 연구(Ⅱ) : On the strand scission ability of plasmid DNA chain by emodin Emodin의 plasmid DNA chain 절단능에 관한 연구

李雄洙 忠州大學校 1999 한국교통대학교 논문집 Vol.34 No.2

It has been reported that several anthraquinone compounds possess mutagenicity and carcinogenicity. Emodin(1, 3, 8-trihydroxy-6-methylanthraquinone)is one of the anthraquinone mycotoxins, and a constituent of several herb medince used as laxative such as Rhei Rhizoma(rhubarb), Cassiae Semen(cassia seed) and Aloe. Emodin was selected as a model compound to solve a carcinogenic mechanism of the anthraquinone mycotoxins and to evaluate the safety of herb medicine. This research was carried out on the genotoxic effects of emodin activated by cytochrome P-450 and on the strand scission ability of plasmid DNA chain by emodin. To investigate the correlation between mutagenicity and DNA strand scission, plasmid DNA pUC19 was treated with emodin and 2-OH-emodin under the various conditionas, and followed the analysis of strand breaks by agarose gel electrophoresis. When emodin and 2-OH-emodin incubated for 1hr at 37℃ , the form Ⅱ(nicked) DNA was slightly increased. In 30min at 65℃, that was remarkably increased. In the presence of reducing agents such as NaBH₄and dithiothreitol(DDT), the formation of the form Ⅱ was slightly accelerated by 2-OH-emodin, and was remakably increased by emodin. Form these results, it was suggested that active oxygens have been generated by the reduction-oxidation cycle such as guinone ?? hydroquinone. The formation of formⅡ induced with emodin decreased by catalase ae H₂O₂quencher, and DMSO and ethanol as hydroxyl radical scavenger in the presence of NaBH₄. To clarify the site of DNA strand sicssion, the nick translation method was introduced, and the radioactivity of[α-³²P] dCMP incorporated into 3'OH terminal of the double strand DNA was counted. The incorporation of [α-³²P] dCMP into emodin-and 2-OH-emodin-treated DNA decreased to 60% and 73% of the controls, respectively. In the presence of NaBH₄, the incorporations were almost same as the control. From these results, it was presumed that the nick site possesses not 3'OH terminal but 3'P. The incorporated radioactivity was increased by treatment of alkaline phospatase in order to dephosphorylate the terminal phosphate residue. It was suggested that active oxygens generated from emodin caused DNA strand scission, and the nick site was presumed to 3'P terminal, not 3'OH. In conclusion, DNA damages by emodin were caused by the fllowing two pathways, i.e emodin is biotransformed into 2-OH-emodin, a direct-acting mutagen by cytochrome P-450, and activated emodin and hydroxyl radical by the reductase. Such approaches are expected to solve the basic mechanism of emodin-induced genotoxicity.

곡물 및 사료중의 Fusarium mycotoxins에 관한 연구(Ⅴ) : - 충주시 지역의 곡물중 trichothecenes,zearalenone 및 fumonisins의 오염 실태 -

李雄洙 충주대 2000 한국교통대학교 논문집 Vol.35 No.2

In order to survey the contamination of Fusarium mycotoxins such as deoxynivalenol(DON), nivalenol (NIV), fusarenon-X(FX), 3-acetyl -DON, zeararenone (ZEN), fumonisin B1(FB1) and B2 (FB2) in cereals harvested in 1999, 61 samples (barley : 15, polished barley : 20, wheat : 10, corn : 16) were collected from the different farms and markets in Chungju city. The results of this survey showed that 87%, 93% and 93% of the barley samples were contaminates with DON, NIV and ZEN, respectively.Average concentrations(㎍/㎏) in barley were 83(DON), 229(NIV) and 55(ZEN).In polished barley samples, incidences of DON, NIV and ZEN were 85%, 90% and 55%, and average concentrations of those were 27ppb,55ppb and 9ppb, respectively.In wheat samples, incidences of those were 60%(DON), 90%(NIV) and 60%(ZEN), and average concentrations of those were 29ppb(DON), 106ppb(NIV) and 28ppb(ZEN).In corn Samples, incidences of DON, NIV, ZEN, FB1 and FB2 were 75%, 81%, 75%, 50% and 44%, and average concentrations of those were 114ppb, 107ppb, 136ppb, 265ppb and 312ppb, respectively.Neither FX nor 3-acetyl-DON was detected from all the samples.FB1 and FB2 were only detected from the corn samples.

여주 건조분말 함유식이가 고콜레스테롤 혈증 Rat의 혈청성분에 미치는 영향

이웅수 忠州大學校 2007 한국교통대학교 논문집 Vol.42 No.-

This research was carried out to investigate the hypocholesterolemic effects of diets containing bitter gourd(Momordica charantia, MC; Yeoju) powder in Sprague-Dawley male rats. After 2 weeks feeding with basal diet, 6 week old rats were fed with basal diet, diets containing 1% cholesterol, and 1% MC, 5% MC and 10% MC powder with 1% cholesterol for 2, 4 and 6 week. And then I measured the levels of glucose, triglyceride, total-, HDL-and LDL-cholesterol, and the activities of GOT, GPT in the serum of rats. The levels of triglyceride and total-cholesterol in the serum of rats were decreased remarkably in the groups fed with diet contained more 5% MC powder compared to the basal diet group for 2weeks. The levels of LDL-cholesterol were decreased in the all groups fed with diet contained MC power for 4weeks, especially groups fed with contained more 5% MC powder were more effective than the other groups, but the decrease of HDL-cholesterol level was not effective in all diet groups contained MC powder. The levels of blood sugar were decreased in all MC powder diet groups, and the decreased levels were more effective in the groups fed with diet contained more MC powder. The activities of GOT and GPT were also decreased in all MC powder diet groups. In conclusion, diets contained MC powder were effective in the decrease of triglyceride, total- and LDL-cholesterol, and glucose levels, and GOT and GPT activities in the serum of rats

토양에서 분리한 세균의 T-2 toxin 분해능

이웅수 충주대학교 2006 한국교통대학교 논문집 Vol.41 No.-

Trichothecenes are a chemically related group of toxic fungal metabolites produced by a number of species of the fungi such as Fusarium, Cephalosporium, Trichothecium, Myrothecium, Stachybotrys and Trichoderma, and are responsible for mycotoxicoses as causative agents in a wide variety of animal and human health problems. T-2 toxin is a potent cytotoxic and immunodepressive trichothecene mycotoxin, and a causative agent of moldy corn toxicosis and alimentary toxic aleukia(ATA). T-2 toxin induces acute toxicity such as leukocytosis, hemorrhage and dermal edema, followed by death in animals. In order to clarify the systems of metabolism, degradation and detoxification of T-2 toxin, one of the naturally occuring trichothecene mycotoxins, this research was carried out. 20 soil samples were examined for the degradation activity of T-2 toxin by incubation of 30℃ for 7 days with medium supplemented 0.2% T-2 toxin as a sole source of carbon and energy by enrichment culture technique. 10 strains that have T-2 toxin degradation activity were isolated from soil samples. Among 10 strains, NO. CJNU-10-1 strain has the most potent activity for the degradation of T-2 toxin. The metabolic pathway of T-2 toxin was investigated with this strain. This strain metabolized T-2 toxin to the following weak or non-toxic compounds ; T-2 toxin→HT-2 toxin→T-2 triol→unknown metabolite(s). The present study has revealed that bacteria, No. CJNU-10-1 strain, isolated from soil has ability of deacetylating, degradation and detoxification T-2 toxin, and a subsequent study for the taxonomical characterization and identification of No. CJNU-10-1 strain is currently under way.